Cucurbita maxima (Pumpkin) (Winter squash)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J1JRM9|A0A6J1JRM9_CUCMA probable metal-nicotianamine transporter YSL7 OS=Cucurbita maxima OX=3661 GN=LOC111486945 PE=4 SV=1
MM1 pKa = 7.18SAVNITNVAVLDD13 pKa = 4.0NPSPFSTPLQFEE25 pKa = 4.21ISYY28 pKa = 10.67EE29 pKa = 4.03CLSPLKK35 pKa = 10.62YY36 pKa = 10.45DD37 pKa = 4.72LEE39 pKa = 4.28WKK41 pKa = 10.29LIYY44 pKa = 10.44VGSAEE49 pKa = 4.41VEE51 pKa = 4.39TYY53 pKa = 10.65DD54 pKa = 4.88QLLEE58 pKa = 4.21CVLVGPVNVGNYY70 pKa = 9.78RR71 pKa = 11.84FVLQADD77 pKa = 3.94PPDD80 pKa = 4.0PSKK83 pKa = 10.94IPEE86 pKa = 3.81EE87 pKa = 4.66DD88 pKa = 3.34IIGVTVLLLTCSYY101 pKa = 9.66MGQEE105 pKa = 5.13FIRR108 pKa = 11.84VGYY111 pKa = 8.86YY112 pKa = 10.66VNNDD116 pKa = 3.14YY117 pKa = 11.42DD118 pKa = 4.51DD119 pKa = 3.85EE120 pKa = 4.33QLRR123 pKa = 11.84EE124 pKa = 4.02EE125 pKa = 4.8PPAKK129 pKa = 10.47VLIDD133 pKa = 3.56RR134 pKa = 11.84VQRR137 pKa = 11.84NILSDD142 pKa = 3.5KK143 pKa = 10.53PRR145 pKa = 11.84VTKK148 pKa = 10.26FPINFYY154 pKa = 10.74PEE156 pKa = 4.25TNDD159 pKa = 3.78SGEE162 pKa = 4.24EE163 pKa = 4.07PPPNDD168 pKa = 3.35PVLAEE173 pKa = 4.24TDD175 pKa = 3.85GDD177 pKa = 4.12DD178 pKa = 4.08QLPASPEE185 pKa = 4.1HH186 pKa = 6.83PSDD189 pKa = 3.54EE190 pKa = 4.36QIPP193 pKa = 3.69

Molecular weight: 21.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J1K8P7|A0A6J1K8P7_CUCMA Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493284 PE=3 SV=1
MM1 pKa = 7.38IASTTLPPWQPTLRR15 pKa = 11.84APLRR19 pKa = 11.84LTRR22 pKa = 11.84TRR24 pKa = 11.84PLVIPLRR31 pKa = 11.84RR32 pKa = 11.84SVGFVQAYY40 pKa = 9.39RR41 pKa = 11.84RR42 pKa = 11.84GGGNNDD48 pKa = 3.29GFGEE52 pKa = 4.28TWDD55 pKa = 3.95KK56 pKa = 10.59VWRR59 pKa = 11.84GANDD63 pKa = 3.49GFEE66 pKa = 4.19KK67 pKa = 10.61FVFEE71 pKa = 4.33ARR73 pKa = 11.84KK74 pKa = 7.19TAEE77 pKa = 3.97RR78 pKa = 11.84LDD80 pKa = 3.44RR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 10.32SVSRR87 pKa = 11.84RR88 pKa = 11.84VSSVAQSAADD98 pKa = 3.78RR99 pKa = 11.84ARR101 pKa = 11.84EE102 pKa = 3.59IDD104 pKa = 3.49RR105 pKa = 11.84EE106 pKa = 4.05FGIGLRR112 pKa = 11.84WRR114 pKa = 11.84NFTLDD119 pKa = 4.23FSRR122 pKa = 11.84NWPRR126 pKa = 11.84YY127 pKa = 8.78RR128 pKa = 11.84RR129 pKa = 11.84QLNQFMDD136 pKa = 3.78TPLGKK141 pKa = 10.7GFVTIFFLWFALSGWLFRR159 pKa = 11.84VLIFATWILPFAGPLLIGTFANSLIIKK186 pKa = 7.28GTCPACNRR194 pKa = 11.84EE195 pKa = 3.66FAGYY199 pKa = 9.49KK200 pKa = 9.31NQIISCTGCGNIVWQPKK217 pKa = 9.06GQGEE221 pKa = 4.12NRR223 pKa = 11.84KK224 pKa = 9.67GGSGSKK230 pKa = 9.53SQPNVIDD237 pKa = 3.56VEE239 pKa = 4.5FEE241 pKa = 3.96EE242 pKa = 5.11KK243 pKa = 10.77

Molecular weight: 27.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26917

7590

34507

16166903

15

5424

468.5

52.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.762 ± 0.011

1.812 ± 0.006

5.36 ± 0.009

6.582 ± 0.014

4.17 ± 0.01

6.62 ± 0.019

2.407 ± 0.005

5.238 ± 0.01

6.028 ± 0.01

9.511 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.005

4.503 ± 0.007

4.987 ± 0.014

3.689 ± 0.009

5.429 ± 0.01

9.411 ± 0.016

4.78 ± 0.007

6.412 ± 0.009

1.218 ± 0.004

2.698 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski