Amino acid dipepetide frequency for Cucurbita maxima (Pumpkin) (Winter squash)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.679AlaAla: 6.679 ± 0.033	1.211AlaCys: 1.211 ± 0.009	3.167AlaAsp: 3.167 ± 0.014	4.267AlaGlu: 4.267 ± 0.02	2.751AlaPhe: 2.751 ± 0.015	4.071AlaGly: 4.071 ± 0.02	1.33AlaHis: 1.33 ± 0.009	3.844AlaIle: 3.844 ± 0.019	3.799AlaLys: 3.799 ± 0.016	6.575AlaLeu: 6.575 ± 0.026	1.799AlaMet: 1.799 ± 0.013	2.632AlaAsn: 2.632 ± 0.014	2.889AlaPro: 2.889 ± 0.017	2.081AlaGln: 2.081 ± 0.014	3.348AlaArg: 3.348 ± 0.016	6.244AlaSer: 6.244 ± 0.023	3.596AlaThr: 3.596 ± 0.017	4.87AlaVal: 4.87 ± 0.021	0.72AlaTrp: 0.72 ± 0.006	1.747AlaTyr: 1.747 ± 0.013	0.001AlaXaa: 0.001 ± 0.0
Cys	0.967CysAla: 0.967 ± 0.01	0.502CysCys: 0.502 ± 0.007	0.85CysAsp: 0.85 ± 0.008	0.911CysGlu: 0.911 ± 0.007	0.86CysPhe: 0.86 ± 0.008	1.357CysGly: 1.357 ± 0.011	0.457CysHis: 0.457 ± 0.006	0.99CysIle: 0.99 ± 0.008	1.101CysLys: 1.101 ± 0.01	1.81CysLeu: 1.81 ± 0.013	0.405CysMet: 0.405 ± 0.006	0.843CysAsn: 0.843 ± 0.008	0.887CysPro: 0.887 ± 0.008	0.576CysGln: 0.576 ± 0.006	1.08CysArg: 1.08 ± 0.009	1.962CysSer: 1.962 ± 0.013	0.815CysThr: 0.815 ± 0.007	0.997CysVal: 0.997 ± 0.008	0.23CysTrp: 0.23 ± 0.004	0.518CysTyr: 0.518 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	3.508AspAla: 3.508 ± 0.015	0.966AspCys: 0.966 ± 0.007	3.708AspAsp: 3.708 ± 0.021	4.127AspGlu: 4.127 ± 0.019	2.32AspPhe: 2.32 ± 0.012	3.982AspGly: 3.982 ± 0.021	1.252AspHis: 1.252 ± 0.01	3.017AspIle: 3.017 ± 0.014	2.723AspLys: 2.723 ± 0.017	5.042AspLeu: 5.042 ± 0.021	1.293AspMet: 1.293 ± 0.01	2.108AspAsn: 2.108 ± 0.013	2.579AspPro: 2.579 ± 0.016	1.78AspGln: 1.78 ± 0.013	2.576AspArg: 2.576 ± 0.017	4.614AspSer: 4.614 ± 0.021	2.192AspThr: 2.192 ± 0.012	3.613AspVal: 3.613 ± 0.015	0.689AspTrp: 0.689 ± 0.007	1.515AspTyr: 1.515 ± 0.012	0.001AspXaa: 0.001 ± 0.0
Glu	4.881GluAla: 4.881 ± 0.02	0.909GluCys: 0.909 ± 0.008	4.044GluAsp: 4.044 ± 0.019	6.397GluGlu: 6.397 ± 0.036	2.422GluPhe: 2.422 ± 0.013	3.711GluGly: 3.711 ± 0.018	1.276GluHis: 1.276 ± 0.01	3.752GluIle: 3.752 ± 0.017	4.832GluLys: 4.832 ± 0.025	6.053GluLeu: 6.053 ± 0.026	1.802GluMet: 1.802 ± 0.013	3.304GluAsn: 3.304 ± 0.019	2.174GluPro: 2.174 ± 0.015	2.23GluGln: 2.23 ± 0.016	3.542GluArg: 3.542 ± 0.022	4.808GluSer: 4.808 ± 0.021	3.151GluThr: 3.151 ± 0.015	4.192GluVal: 4.192 ± 0.022	0.699GluTrp: 0.699 ± 0.007	1.641GluTyr: 1.641 ± 0.015	0.001GluXaa: 0.001 ± 0.0
Phe	2.397PheAla: 2.397 ± 0.015	0.908PheCys: 0.908 ± 0.009	2.376PheAsp: 2.376 ± 0.012	2.345PheGlu: 2.345 ± 0.012	1.965PhePhe: 1.965 ± 0.013	3.065PheGly: 3.065 ± 0.02	1.169PheHis: 1.169 ± 0.01	2.05PheIle: 2.05 ± 0.013	2.064PheLys: 2.064 ± 0.013	4.234PheLeu: 4.234 ± 0.021	0.93PheMet: 0.93 ± 0.008	1.752PheAsn: 1.752 ± 0.011	2.143PhePro: 2.143 ± 0.014	1.581PheGln: 1.581 ± 0.01	2.127PheArg: 2.127 ± 0.013	4.226PheSer: 4.226 ± 0.018	1.925PheThr: 1.925 ± 0.011	2.695PheVal: 2.695 ± 0.013	0.548PheTrp: 0.548 ± 0.006	1.197PheTyr: 1.197 ± 0.01	0.001PheXaa: 0.001 ± 0.0
Gly	3.926GlyAla: 3.926 ± 0.026	1.279GlyCys: 1.279 ± 0.01	3.435GlyAsp: 3.435 ± 0.015	3.685GlyGlu: 3.685 ± 0.019	3.103GlyPhe: 3.103 ± 0.016	6.315GlyGly: 6.315 ± 0.096	1.535GlyHis: 1.535 ± 0.012	3.567GlyIle: 3.567 ± 0.017	4.107GlyLys: 4.107 ± 0.02	5.755GlyLeu: 5.755 ± 0.023	1.508GlyMet: 1.508 ± 0.011	3.238GlyAsn: 3.238 ± 0.018	2.317GlyPro: 2.317 ± 0.015	2.021GlyGln: 2.021 ± 0.012	3.787GlyArg: 3.787 ± 0.018	6.334GlySer: 6.334 ± 0.025	3.129GlyThr: 3.129 ± 0.014	4.11GlyVal: 4.11 ± 0.02	0.865GlyTrp: 0.865 ± 0.008	2.184GlyTyr: 2.184 ± 0.032	0.001GlyXaa: 0.001 ± 0.0
His	1.373HisAla: 1.373 ± 0.01	0.505HisCys: 0.505 ± 0.007	1.184HisAsp: 1.184 ± 0.01	1.461HisGlu: 1.461 ± 0.019	1.057HisPhe: 1.057 ± 0.009	1.766HisGly: 1.766 ± 0.014	0.983HisHis: 0.983 ± 0.013	1.181HisIle: 1.181 ± 0.009	1.169HisLys: 1.169 ± 0.009	2.4HisLeu: 2.4 ± 0.013	0.535HisMet: 0.535 ± 0.006	0.983HisAsn: 0.983 ± 0.008	1.421HisPro: 1.421 ± 0.01	1.058HisGln: 1.058 ± 0.009	1.42HisArg: 1.42 ± 0.01	2.139HisSer: 2.139 ± 0.013	0.972HisThr: 0.972 ± 0.009	1.488HisVal: 1.488 ± 0.01	0.293HisTrp: 0.293 ± 0.004	0.679HisTyr: 0.679 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	3.568IleAla: 3.568 ± 0.017	1.098IleCys: 1.098 ± 0.009	2.919IleAsp: 2.919 ± 0.015	3.273IleGlu: 3.273 ± 0.015	2.265IlePhe: 2.265 ± 0.013	3.367IleGly: 3.367 ± 0.016	1.303IleHis: 1.303 ± 0.01	2.664IleIle: 2.664 ± 0.015	2.839IleLys: 2.839 ± 0.014	5.121IleLeu: 5.121 ± 0.025	1.129IleMet: 1.129 ± 0.008	2.165IleAsn: 2.165 ± 0.012	2.914IlePro: 2.914 ± 0.016	2.009IleGln: 2.009 ± 0.012	2.678IleArg: 2.678 ± 0.014	5.098IleSer: 5.098 ± 0.02	2.472IleThr: 2.472 ± 0.012	3.409IleVal: 3.409 ± 0.017	0.665IleTrp: 0.665 ± 0.007	1.417IleTyr: 1.417 ± 0.011	0.001IleXaa: 0.001 ± 0.0
Lys	4.001LysAla: 4.001 ± 0.016	0.943LysCys: 0.943 ± 0.008	3.244LysAsp: 3.244 ± 0.015	4.569LysGlu: 4.569 ± 0.025	2.213LysPhe: 2.213 ± 0.013	3.426LysGly: 3.426 ± 0.016	1.43LysHis: 1.43 ± 0.017	3.166LysIle: 3.166 ± 0.015	4.601LysLys: 4.601 ± 0.027	5.886LysLeu: 5.886 ± 0.025	1.483LysMet: 1.483 ± 0.011	2.775LysAsn: 2.775 ± 0.013	2.774LysPro: 2.774 ± 0.017	2.286LysGln: 2.286 ± 0.012	3.664LysArg: 3.664 ± 0.018	4.825LysSer: 4.825 ± 0.024	2.852LysThr: 2.852 ± 0.016	3.749LysVal: 3.749 ± 0.017	0.78LysTrp: 0.78 ± 0.007	1.61LysTyr: 1.61 ± 0.011	0.001LysXaa: 0.001 ± 0.0
Leu	6.366LeuAla: 6.366 ± 0.024	1.801LeuCys: 1.801 ± 0.012	5.023LeuAsp: 5.023 ± 0.023	6.35LeuGlu: 6.35 ± 0.027	3.778LeuPhe: 3.778 ± 0.021	5.634LeuGly: 5.634 ± 0.021	2.593LeuHis: 2.593 ± 0.015	4.528LeuIle: 4.528 ± 0.018	6.127LeuLys: 6.127 ± 0.024	9.718LeuLeu: 9.718 ± 0.041	2.067LeuMet: 2.067 ± 0.011	4.042LeuAsn: 4.042 ± 0.017	5.146LeuPro: 5.146 ± 0.019	4.382LeuGln: 4.382 ± 0.019	5.333LeuArg: 5.333 ± 0.022	8.585LeuSer: 8.585 ± 0.031	4.229LeuThr: 4.229 ± 0.019	5.986LeuVal: 5.986 ± 0.023	1.074LeuTrp: 1.074 ± 0.008	2.349LeuTyr: 2.349 ± 0.014	0.002LeuXaa: 0.002 ± 0.0
Met	2.204MetAla: 2.204 ± 0.011	0.292MetCys: 0.292 ± 0.004	1.437MetAsp: 1.437 ± 0.01	2.045MetGlu: 2.045 ± 0.013	0.82MetPhe: 0.82 ± 0.007	1.613MetGly: 1.613 ± 0.012	0.512MetHis: 0.512 ± 0.006	1.173MetIle: 1.173 ± 0.01	1.636MetLys: 1.636 ± 0.012	2.065MetLeu: 2.065 ± 0.012	0.632MetMet: 0.632 ± 0.007	1.091MetAsn: 1.091 ± 0.008	1.034MetPro: 1.034 ± 0.008	0.916MetGln: 0.916 ± 0.009	1.164MetArg: 1.164 ± 0.008	1.77MetSer: 1.77 ± 0.011	1.011MetThr: 1.011 ± 0.007	1.601MetVal: 1.601 ± 0.01	0.245MetTrp: 0.245 ± 0.004	0.55MetTyr: 0.55 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.739AsnAla: 2.739 ± 0.013	0.853AsnCys: 0.853 ± 0.008	2.246AsnAsp: 2.246 ± 0.013	2.698AsnGlu: 2.698 ± 0.015	2.018AsnPhe: 2.018 ± 0.011	3.457AsnGly: 3.457 ± 0.017	1.162AsnHis: 1.162 ± 0.009	2.385AsnIle: 2.385 ± 0.015	2.298AsnLys: 2.298 ± 0.013	4.479AsnLeu: 4.479 ± 0.02	1.077AsnMet: 1.077 ± 0.009	2.229AsnAsn: 2.229 ± 0.016	2.404AsnPro: 2.404 ± 0.012	1.79AsnGln: 1.79 ± 0.013	2.166AsnArg: 2.166 ± 0.012	4.317AsnSer: 4.317 ± 0.018	1.928AsnThr: 1.928 ± 0.015	2.867AsnVal: 2.867 ± 0.014	0.575AsnTrp: 0.575 ± 0.007	1.336AsnTyr: 1.336 ± 0.013	0.001AsnXaa: 0.001 ± 0.0
Pro	3.013ProAla: 3.013 ± 0.016	0.787ProCys: 0.787 ± 0.008	2.443ProAsp: 2.443 ± 0.013	3.097ProGlu: 3.097 ± 0.015	2.043ProPhe: 2.043 ± 0.012	2.623ProGly: 2.623 ± 0.015	1.133ProHis: 1.133 ± 0.009	2.414ProIle: 2.414 ± 0.013	2.72ProLys: 2.72 ± 0.015	4.404ProLeu: 4.404 ± 0.018	1.013ProMet: 1.013 ± 0.008	2.34ProAsn: 2.34 ± 0.014	4.437ProPro: 4.437 ± 0.073	1.828ProGln: 1.828 ± 0.014	2.415ProArg: 2.415 ± 0.016	5.52ProSer: 5.52 ± 0.025	2.671ProThr: 2.671 ± 0.017	3.106ProVal: 3.106 ± 0.016	0.588ProTrp: 0.588 ± 0.006	1.273ProTyr: 1.273 ± 0.012	0.002ProXaa: 0.002 ± 0.0
Gln	2.393GlnAla: 2.393 ± 0.014	0.575GlnCys: 0.575 ± 0.007	1.673GlnAsp: 1.673 ± 0.011	2.367GlnGlu: 2.367 ± 0.015	1.408GlnPhe: 1.408 ± 0.01	2.015GlnGly: 2.015 ± 0.015	0.913GlnHis: 0.913 ± 0.008	2.061GlnIle: 2.061 ± 0.013	2.353GlnLys: 2.353 ± 0.013	3.612GlnLeu: 3.612 ± 0.017	1.016GlnMet: 1.016 ± 0.009	1.857GlnAsn: 1.857 ± 0.013	1.858GlnPro: 1.858 ± 0.015	2.122GlnGln: 2.122 ± 0.032	2.158GlnArg: 2.158 ± 0.011	3.089GlnSer: 3.089 ± 0.018	1.77GlnThr: 1.77 ± 0.012	2.257GlnVal: 2.257 ± 0.013	0.464GlnTrp: 0.464 ± 0.006	0.93GlnTyr: 0.93 ± 0.007	0.001GlnXaa: 0.001 ± 0.0
Arg	3.325ArgAla: 3.325 ± 0.014	0.961ArgCys: 0.961 ± 0.007	2.758ArgAsp: 2.758 ± 0.019	3.418ArgGlu: 3.418 ± 0.02	2.243ArgPhe: 2.243 ± 0.014	3.275ArgGly: 3.275 ± 0.018	1.323ArgHis: 1.323 ± 0.01	2.908ArgIle: 2.908 ± 0.015	3.911ArgLys: 3.911 ± 0.018	5.026ArgLeu: 5.026 ± 0.021	1.302ArgMet: 1.302 ± 0.01	2.596ArgAsn: 2.596 ± 0.014	2.425ArgPro: 2.425 ± 0.014	1.903ArgGln: 1.903 ± 0.011	4.182ArgArg: 4.182 ± 0.027	4.766ArgSer: 4.766 ± 0.023	2.605ArgThr: 2.605 ± 0.015	3.217ArgVal: 3.217 ± 0.015	0.723ArgTrp: 0.723 ± 0.006	1.422ArgTyr: 1.422 ± 0.01	0.001ArgXaa: 0.001 ± 0.0
Ser	5.615SerAla: 5.615 ± 0.022	1.762SerCys: 1.762 ± 0.012	4.728SerAsp: 4.728 ± 0.022	5.114SerGlu: 5.114 ± 0.022	4.086SerPhe: 4.086 ± 0.019	6.182SerGly: 6.182 ± 0.024	2.143SerHis: 2.143 ± 0.012	4.724SerIle: 4.724 ± 0.022	5.164SerLys: 5.164 ± 0.021	8.752SerLeu: 8.752 ± 0.032	2.196SerMet: 2.196 ± 0.013	4.419SerAsn: 4.419 ± 0.02	5.014SerPro: 5.014 ± 0.038	3.197SerGln: 3.197 ± 0.019	4.884SerArg: 4.884 ± 0.024	12.332SerSer: 12.332 ± 0.057	4.885SerThr: 4.885 ± 0.02	5.485SerVal: 5.485 ± 0.022	1.13SerTrp: 1.13 ± 0.009	2.296SerTyr: 2.296 ± 0.012	0.002SerXaa: 0.002 ± 0.0
Thr	3.47ThrAla: 3.47 ± 0.018	0.862ThrCys: 0.862 ± 0.007	2.33ThrAsp: 2.33 ± 0.011	2.841ThrGlu: 2.841 ± 0.014	2.013ThrPhe: 2.013 ± 0.012	3.148ThrGly: 3.148 ± 0.015	1.041ThrHis: 1.041 ± 0.008	2.647ThrIle: 2.647 ± 0.013	2.633ThrLys: 2.633 ± 0.015	4.405ThrLeu: 4.405 ± 0.018	1.164ThrMet: 1.164 ± 0.012	2.135ThrAsn: 2.135 ± 0.011	2.637ThrPro: 2.637 ± 0.017	1.475ThrGln: 1.475 ± 0.011	2.369ThrArg: 2.369 ± 0.014	4.646ThrSer: 4.646 ± 0.021	2.862ThrThr: 2.862 ± 0.016	3.299ThrVal: 3.299 ± 0.018	0.568ThrTrp: 0.568 ± 0.006	1.254ThrTyr: 1.254 ± 0.01	0.001ThrXaa: 0.001 ± 0.0
Val	4.744ValAla: 4.744 ± 0.019	1.108ValCys: 1.108 ± 0.009	3.786ValAsp: 3.786 ± 0.016	4.543ValGlu: 4.543 ± 0.02	2.614ValPhe: 2.614 ± 0.013	4.159ValGly: 4.159 ± 0.019	1.535ValHis: 1.535 ± 0.01	3.262ValIle: 3.262 ± 0.015	3.828ValLys: 3.828 ± 0.017	6.076ValLeu: 6.076 ± 0.021	1.456ValMet: 1.456 ± 0.01	2.585ValAsn: 2.585 ± 0.014	3.143ValPro: 3.143 ± 0.016	2.322ValGln: 2.322 ± 0.013	3.096ValArg: 3.096 ± 0.014	5.548ValSer: 5.548 ± 0.02	2.954ValThr: 2.954 ± 0.016	4.793ValVal: 4.793 ± 0.022	0.733ValTrp: 0.733 ± 0.007	1.832ValTyr: 1.832 ± 0.014	0.001ValXaa: 0.001 ± 0.0
Trp	0.743TrpAla: 0.743 ± 0.008	0.218TrpCys: 0.218 ± 0.004	0.664TrpAsp: 0.664 ± 0.008	0.734TrpGlu: 0.734 ± 0.007	0.509TrpPhe: 0.509 ± 0.006	0.709TrpGly: 0.709 ± 0.009	0.284TrpHis: 0.284 ± 0.004	0.681TrpIle: 0.681 ± 0.007	0.909TrpLys: 0.909 ± 0.008	1.133TrpLeu: 1.133 ± 0.01	0.336TrpMet: 0.336 ± 0.005	0.688TrpAsn: 0.688 ± 0.007	0.49TrpPro: 0.49 ± 0.006	0.414TrpGln: 0.414 ± 0.005	0.811TrpArg: 0.811 ± 0.008	0.986TrpSer: 0.986 ± 0.008	0.612TrpThr: 0.612 ± 0.006	0.732TrpVal: 0.732 ± 0.008	0.224TrpTrp: 0.224 ± 0.004	0.308TrpTyr: 0.308 ± 0.004	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.707TyrAla: 1.707 ± 0.01	0.578TyrCys: 0.578 ± 0.007	1.588TyrAsp: 1.588 ± 0.016	1.578TyrGlu: 1.578 ± 0.012	1.209TyrPhe: 1.209 ± 0.01	2.324TyrGly: 2.324 ± 0.035	0.696TyrHis: 0.696 ± 0.007	1.36TyrIle: 1.36 ± 0.011	1.462TyrLys: 1.462 ± 0.014	2.563TyrLeu: 2.563 ± 0.014	0.67TyrMet: 0.67 ± 0.007	1.243TyrAsn: 1.243 ± 0.01	1.181TyrPro: 1.181 ± 0.009	0.919TyrGln: 0.919 ± 0.007	1.491TyrArg: 1.491 ± 0.01	2.296TyrSer: 2.296 ± 0.013	1.171TyrThr: 1.171 ± 0.009	1.652TyrVal: 1.652 ± 0.012	0.371TyrTrp: 0.371 ± 0.005	0.922TyrTyr: 0.922 ± 0.013	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.002XaaLeu: 0.002 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.002XaaSer: 0.002 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.003XaaXaa: 0.003 ± 0.003

Statistics based on 34507 proteins (16166903 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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