Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella; Legionella pneumophila; Legionella pneumophila subsp. pneumophila

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5ZXV6|Q5ZXV6_LEGPH Expressed protein OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) OX=272624 GN=lpg0625 PE=4 SV=1
MM1 pKa = 7.32LAEE4 pKa = 4.1SVIGIVRR11 pKa = 11.84AVNGLLEE18 pKa = 4.23KK19 pKa = 11.24VNVQGQASLVKK30 pKa = 10.27SGARR34 pKa = 11.84LEE36 pKa = 4.47EE37 pKa = 4.9GDD39 pKa = 4.43VLTLLSGEE47 pKa = 4.59AYY49 pKa = 9.54IQFIHH54 pKa = 6.82GFPEE58 pKa = 3.96ALALEE63 pKa = 4.75KK64 pKa = 10.12PVKK67 pKa = 10.35LDD69 pKa = 3.42GVSPTLQYY77 pKa = 10.98GAEE80 pKa = 4.01DD81 pKa = 3.65LKK83 pKa = 11.38EE84 pKa = 4.03QMVQEE89 pKa = 5.21AIAKK93 pKa = 10.16GIDD96 pKa = 3.28PSVILDD102 pKa = 3.73VLGSAAAGAVAVGSGGDD119 pKa = 3.52AFIIDD124 pKa = 3.81PLFGFGHH131 pKa = 5.4VTAGYY136 pKa = 6.21PTGPISFAYY145 pKa = 9.89EE146 pKa = 3.85ADD148 pKa = 3.73TQQLFWFVPEE158 pKa = 3.91EE159 pKa = 3.86TGAIAEE165 pKa = 4.49SEE167 pKa = 4.25LTTEE171 pKa = 5.02PEE173 pKa = 4.13FTPQIPQFTTNQAVLTVFEE192 pKa = 5.06DD193 pKa = 3.97ALPSGIPDD201 pKa = 3.25SAGQARR207 pKa = 11.84TASSSLSTLLTSSPDD222 pKa = 3.34VAASFAFNTNLSVLPTLKK240 pKa = 10.65SGGIDD245 pKa = 3.45LDD247 pKa = 4.45YY248 pKa = 11.36NLSPDD253 pKa = 3.6KK254 pKa = 10.39RR255 pKa = 11.84TLTASIPGGGDD266 pKa = 3.04VMQFEE271 pKa = 4.77LTADD275 pKa = 3.41GRR277 pKa = 11.84LTQTLMDD284 pKa = 5.44SIDD287 pKa = 3.83HH288 pKa = 6.72PTTDD292 pKa = 3.53SDD294 pKa = 3.58DD295 pKa = 4.49GEE297 pKa = 4.35WMRR300 pKa = 11.84LDD302 pKa = 4.61LSPLIYY308 pKa = 10.12VTFTRR313 pKa = 11.84TSDD316 pKa = 3.52GTVLEE321 pKa = 4.53SRR323 pKa = 11.84TLPANAVVAGIQDD336 pKa = 3.76DD337 pKa = 4.34VPIARR342 pKa = 11.84AQLTNNEE349 pKa = 4.06ILLDD353 pKa = 3.6EE354 pKa = 4.4TVGVKK359 pKa = 10.48VGDD362 pKa = 3.68ADD364 pKa = 4.66AANDD368 pKa = 4.25DD369 pKa = 4.42FNPTITTDD377 pKa = 3.65PFNNTYY383 pKa = 10.4GIPIGLVQNANLLDD397 pKa = 4.11NGTSEE402 pKa = 4.66MGGDD406 pKa = 3.71YY407 pKa = 11.03KK408 pKa = 11.05NATMTHH414 pKa = 6.62LLKK417 pKa = 9.93ITDD420 pKa = 3.99AVSGLQTTDD429 pKa = 2.73GTSINLFLEE438 pKa = 4.43SNGDD442 pKa = 3.06ITGRR446 pKa = 11.84AGDD449 pKa = 3.44IGEE452 pKa = 4.26PAVFAIRR459 pKa = 11.84MNPNTGSITVAQYY472 pKa = 11.6GSIKK476 pKa = 10.54QFDD479 pKa = 4.28TNSHH483 pKa = 6.79DD484 pKa = 3.79EE485 pKa = 4.28AVDD488 pKa = 3.48LTGRR492 pKa = 11.84ISAVVTAKK500 pKa = 10.92DD501 pKa = 3.15SDD503 pKa = 3.89GDD505 pKa = 3.76VSNVEE510 pKa = 3.87IPIGQLIIFADD521 pKa = 4.47DD522 pKa = 4.35GPSVTMAVSDD532 pKa = 4.01NNAITLNTQDD542 pKa = 4.44ADD544 pKa = 4.34TIGAASDD551 pKa = 3.51SDD553 pKa = 4.01SASFAAAFAVTPNYY567 pKa = 10.22GADD570 pKa = 3.64GAGTTVTTYY579 pKa = 11.08ALSVSAQGVDD589 pKa = 3.71SGLDD593 pKa = 3.28NNGNNIYY600 pKa = 9.96LYY602 pKa = 10.07NIAGSVVGSTSATQAGITTGNTIFSLDD629 pKa = 3.69VNSSSGVVTLTQHH642 pKa = 5.98QEE644 pKa = 3.71VDD646 pKa = 3.11HH647 pKa = 6.39GLPGASSNYY656 pKa = 9.21AAQEE660 pKa = 4.5AILNTGLVFLNATAVTTDD678 pKa = 3.21GDD680 pKa = 4.1GDD682 pKa = 3.96TATASASLDD691 pKa = 3.2LGGNVKK697 pKa = 10.18FDD699 pKa = 4.29DD700 pKa = 5.2DD701 pKa = 4.5GPSVTMAVSDD711 pKa = 4.01NNAITLNTQDD721 pKa = 4.44ADD723 pKa = 4.34TIGAASDD730 pKa = 3.51SDD732 pKa = 4.01SASFAAAFAVTPNYY746 pKa = 10.22GADD749 pKa = 3.64GAGTTVTTYY758 pKa = 11.08ALSVSAQGVDD768 pKa = 3.71SGLDD772 pKa = 3.28NNGNNIYY779 pKa = 9.96LYY781 pKa = 10.07NIAGSVVGSTSATQAGITTGNTIFSLDD808 pKa = 3.69VNSSSGVVTLTQHH821 pKa = 5.98QEE823 pKa = 3.71VDD825 pKa = 3.11HH826 pKa = 6.39GLPGASSNYY835 pKa = 9.21AAQEE839 pKa = 4.5AILNTGLVFLNATAVTTDD857 pKa = 3.21GDD859 pKa = 4.1GDD861 pKa = 3.96TATASASLDD870 pKa = 3.2LGGNVKK876 pKa = 10.18FDD878 pKa = 4.29DD879 pKa = 5.2DD880 pKa = 4.5GPSVTMAVSDD890 pKa = 4.01NNAITLNTQDD900 pKa = 4.44ADD902 pKa = 4.34TIGAASDD909 pKa = 3.51SDD911 pKa = 4.01SASFAAAFAVTPNYY925 pKa = 10.22GADD928 pKa = 3.64GAGTTVTTYY937 pKa = 11.08ALSVSAQGVDD947 pKa = 3.71SGLDD951 pKa = 3.28NNGNNIYY958 pKa = 9.96LYY960 pKa = 10.07NIAGSVVGSTSATQAGITTGNTIFSLDD987 pKa = 3.69VNSSSGVVTLTQHH1000 pKa = 5.98QEE1002 pKa = 3.71VDD1004 pKa = 3.11HH1005 pKa = 6.39GLPGASSNYY1014 pKa = 9.21AAQEE1018 pKa = 4.5AILNTGLVFLNATAVTTDD1036 pKa = 3.21GDD1038 pKa = 4.1GDD1040 pKa = 3.96TATASASLDD1049 pKa = 3.2LGGNVKK1055 pKa = 10.18FDD1057 pKa = 4.29DD1058 pKa = 5.2DD1059 pKa = 4.5GPSVTMAVSDD1069 pKa = 4.01NNAITLNTQDD1079 pKa = 4.06AEE1081 pKa = 4.71TIGVLSDD1088 pKa = 3.14TDD1090 pKa = 3.87SASFAAAFAVTPNYY1104 pKa = 10.22GADD1107 pKa = 3.64GAGTTVTTYY1116 pKa = 11.08ALSVSAQGVDD1126 pKa = 3.71SGLDD1130 pKa = 3.28NNGNNIYY1137 pKa = 9.96LYY1139 pKa = 10.07NIAGSVVGSTSATQAGITTGNTIFSLDD1166 pKa = 3.69VNSSSGVVTLTQHH1179 pKa = 5.98QEE1181 pKa = 3.71VDD1183 pKa = 3.11HH1184 pKa = 6.39GLPGASSNYY1193 pKa = 9.21AAQEE1197 pKa = 4.5AILNTGLVFLNATAVTTDD1215 pKa = 3.21GDD1217 pKa = 4.1GDD1219 pKa = 3.96TATASASLDD1228 pKa = 3.2LGGNVKK1234 pKa = 10.18FDD1236 pKa = 4.29DD1237 pKa = 5.2DD1238 pKa = 4.5GPSVTMAVSDD1248 pKa = 4.01NNAITLNTQDD1258 pKa = 4.44ADD1260 pKa = 4.34TIGAASDD1267 pKa = 3.51SDD1269 pKa = 4.01SASFAAAFAVTPNYY1283 pKa = 10.22GADD1286 pKa = 3.64GAGTTVTTYY1295 pKa = 11.08ALSVSAQGVDD1305 pKa = 3.71SGLDD1309 pKa = 3.28NNGNNIYY1316 pKa = 9.96LYY1318 pKa = 10.07NIAGSVVGSTSATQAGITTGNTIFSLDD1345 pKa = 3.69VNSSSGVVTLTQHH1358 pKa = 5.98QEE1360 pKa = 3.71VDD1362 pKa = 3.11HH1363 pKa = 6.39GLPGASSNYY1372 pKa = 9.21AAQEE1376 pKa = 4.5AILNTGLVFLNATAVTTDD1394 pKa = 3.21GDD1396 pKa = 4.1GDD1398 pKa = 3.96TATASASLDD1407 pKa = 3.2LGGNVKK1413 pKa = 10.18FDD1415 pKa = 4.29DD1416 pKa = 5.2DD1417 pKa = 4.5GPSVTMAVSDD1427 pKa = 4.01NNAITLNTQDD1437 pKa = 4.44ADD1439 pKa = 4.34TIGAASDD1446 pKa = 3.51SDD1448 pKa = 4.01SASFAAAFAVTPNYY1462 pKa = 10.22GADD1465 pKa = 3.64GAGTTVTTYY1474 pKa = 10.93ALSVSAPRR1482 pKa = 11.84GGFRR1486 pKa = 11.84PP1487 pKa = 3.7

Molecular weight: 150.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5ZR83|Q5ZR83_LEGPH Uncharacterized protein OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) OX=272624 GN=lpg3000 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.18RR12 pKa = 11.84KK13 pKa = 8.9RR14 pKa = 11.84DD15 pKa = 3.28HH16 pKa = 6.64GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTRR25 pKa = 11.84AGRR28 pKa = 11.84LVIKK32 pKa = 10.41RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2930

0

2930

997422

34

3780

340.4

38.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.198 ± 0.048

1.21 ± 0.018

4.821 ± 0.033

6.031 ± 0.05

4.501 ± 0.03

6.035 ± 0.044

2.464 ± 0.021

7.628 ± 0.042

6.507 ± 0.044

10.957 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.019

5.01 ± 0.032

4.062 ± 0.028

4.536 ± 0.037

4.161 ± 0.033

6.68 ± 0.034

5.137 ± 0.029

5.88 ± 0.039

1.151 ± 0.017

3.567 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski