Felis catus papillomavirus 4
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T2D2T1|T2D2T1_9PAPI Major capsid protein L1 OS=Felis catus papillomavirus 4 OX=1398507 GN=L1 PE=3 SV=1
MM1 pKa = 7.51 IGKK4 pKa = 8.89 EE5 pKa = 3.94 PQIKK9 pKa = 10.27 DD10 pKa = 3.04 IEE12 pKa = 5.04 LDD14 pKa = 3.47 LDD16 pKa = 4.1 EE17 pKa = 5.82 LVLPANLLASEE28 pKa = 5.1 EE29 pKa = 3.97 ILEE32 pKa = 4.37 AEE34 pKa = 4.31 EE35 pKa = 4.28 EE36 pKa = 4.4 EE37 pKa = 4.52 EE38 pKa = 4.67 PEE40 pKa = 4.28 SNPYY44 pKa = 10.3 RR45 pKa = 11.84 IVTCCVLCHH54 pKa = 5.24 STLRR58 pKa = 11.84 LVVSATDD65 pKa = 3.44 AQIRR69 pKa = 11.84 AQQDD73 pKa = 3.28 LFLAGLGIICPVCYY87 pKa = 10.12 RR88 pKa = 11.84 NNRR91 pKa = 11.84 NHH93 pKa = 7.32 GGQQGKK99 pKa = 9.6 RR100 pKa = 11.84 PP101 pKa = 3.63
Molecular weight: 11.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.566
IPC2_protein 4.673
IPC_protein 4.52
Toseland 4.393
ProMoST 4.622
Dawson 4.457
Bjellqvist 4.609
Wikipedia 4.304
Rodwell 4.368
Grimsley 4.304
Solomon 4.457
Lehninger 4.406
Nozaki 4.571
DTASelect 4.66
Thurlkill 4.393
EMBOSS 4.329
Sillero 4.635
Patrickios 3.503
IPC_peptide 4.469
IPC2_peptide 4.635
IPC2.peptide.svr19 4.561
Protein with the highest isoelectric point:
>tr|T2D3L1|T2D3L1_9PAPI Putative E4 protein OS=Felis catus papillomavirus 4 OX=1398507 GN=E4 PE=4 SV=1
MM1 pKa = 7.71 EE2 pKa = 4.95 NLSTRR7 pKa = 11.84 FDD9 pKa = 3.69 TLQEE13 pKa = 3.93 VLLAHH18 pKa = 5.91 YY19 pKa = 9.33 EE20 pKa = 4.03 KK21 pKa = 10.48 DD22 pKa = 3.41 SKK24 pKa = 11.18 KK25 pKa = 9.39 ITDD28 pKa = 3.82 HH29 pKa = 5.96 VSFWEE34 pKa = 3.78 LLRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 4.45 SVMLHH44 pKa = 5.78 YY45 pKa = 10.61 ARR47 pKa = 11.84 QQGISSLGFFQVPSLQVSEE66 pKa = 5.19 AKK68 pKa = 10.01 AKK70 pKa = 9.73 KK71 pKa = 9.98 AIMMSLMLQQLAKK84 pKa = 9.98 TPFGQEE90 pKa = 3.64 PWSMTEE96 pKa = 3.71 TSLEE100 pKa = 4.02 MLEE103 pKa = 4.84 APPKK107 pKa = 10.63 GKK109 pKa = 9.91 FKK111 pKa = 10.76 KK112 pKa = 10.42 GPRR115 pKa = 11.84 TVEE118 pKa = 3.46 VWFDD122 pKa = 3.81 NNPDD126 pKa = 3.05 NSFPYY131 pKa = 10.15 TSWTCIYY138 pKa = 10.23 IQDD141 pKa = 5.57 AEE143 pKa = 4.48 DD144 pKa = 3.17 TWHH147 pKa = 6.58 KK148 pKa = 11.3 VEE150 pKa = 4.93 GLVDD154 pKa = 3.9 YY155 pKa = 10.78 EE156 pKa = 3.79 GLYY159 pKa = 10.74 YY160 pKa = 10.34 VDD162 pKa = 4.82 CDD164 pKa = 4.49 GEE166 pKa = 3.97 VHH168 pKa = 6.46 YY169 pKa = 10.21 YY170 pKa = 10.54 VKK172 pKa = 10.5 FAADD176 pKa = 3.43 ALQYY180 pKa = 9.54 ATTGMWRR187 pKa = 11.84 VNYY190 pKa = 9.98 KK191 pKa = 9.96 NQTISASVSSSSSEE205 pKa = 3.78 DD206 pKa = 3.25 QQRR209 pKa = 11.84 QGQQQQQQQQQPSTTTSVEE228 pKa = 3.82 WPQRR232 pKa = 11.84 PSSSGLQHH240 pKa = 6.83 EE241 pKa = 5.16 SPFTPRR247 pKa = 11.84 GHH249 pKa = 6.69 GRR251 pKa = 11.84 GCVRR255 pKa = 11.84 GRR257 pKa = 11.84 RR258 pKa = 11.84 SSGSSPHH265 pKa = 5.4 QRR267 pKa = 11.84 GDD269 pKa = 2.76 RR270 pKa = 11.84 GGRR273 pKa = 11.84 GRR275 pKa = 11.84 SQSPSTPTTPGALPPDD291 pKa = 4.07 LSFAGGDD298 pKa = 3.22 RR299 pKa = 11.84 SGGGGGGGGGGRR311 pKa = 11.84 QRR313 pKa = 11.84 GRR315 pKa = 11.84 RR316 pKa = 11.84 GSRR319 pKa = 11.84 GPPRR323 pKa = 11.84 LSPYY327 pKa = 9.95 IPLGQVEE334 pKa = 4.43 QGPGRR339 pKa = 11.84 PEE341 pKa = 3.67 GAALQRR347 pKa = 11.84 PGRR350 pKa = 11.84 PEE352 pKa = 3.59 KK353 pKa = 10.75 NPRR356 pKa = 11.84 LSSTTPVVVLKK367 pKa = 11.19 GPGNALKK374 pKa = 10.35 CWRR377 pKa = 11.84 LRR379 pKa = 11.84 AKK381 pKa = 10.1 AKK383 pKa = 10.45 HH384 pKa = 5.65 GALFCAISTAFSWVEE399 pKa = 3.66 KK400 pKa = 10.25 SSSSRR405 pKa = 11.84 IGRR408 pKa = 11.84 HH409 pKa = 5.7 RR410 pKa = 11.84 ILVGFINEE418 pKa = 4.0 EE419 pKa = 3.71 QRR421 pKa = 11.84 EE422 pKa = 4.23 DD423 pKa = 3.46 FLRR426 pKa = 11.84 TVRR429 pKa = 11.84 LPRR432 pKa = 11.84 GVEE435 pKa = 4.12 CVPGGLDD442 pKa = 3.5 SLL444 pKa = 4.5
Molecular weight: 49.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.919
IPC2_protein 8.873
IPC_protein 8.858
Toseland 9.487
ProMoST 9.297
Dawson 9.78
Bjellqvist 9.545
Wikipedia 9.955
Rodwell 9.984
Grimsley 9.853
Solomon 9.809
Lehninger 9.765
Nozaki 9.648
DTASelect 9.487
Thurlkill 9.619
EMBOSS 9.926
Sillero 9.736
Patrickios 5.194
IPC_peptide 9.809
IPC2_peptide 8.302
IPC2.peptide.svr19 7.858
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2507
101
608
358.1
39.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.465 ± 0.372
2.553 ± 0.826
5.704 ± 0.53
6.462 ± 0.469
4.108 ± 0.625
8.935 ± 1.523
1.635 ± 0.159
3.47 ± 0.348
4.826 ± 0.805
8.895 ± 0.712
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.715 ± 0.268
3.391 ± 0.592
7.06 ± 1.106
4.906 ± 0.676
6.223 ± 0.751
8.496 ± 0.657
5.744 ± 0.559
6.223 ± 0.586
1.316 ± 0.344
2.832 ± 0.514
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here