Nicotiana attenuata (Coyote tobacco)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J6L408|A0A1J6L408_NICAT Protein mut11 OS=Nicotiana attenuata OX=49451 GN=MUT11_0 PE=3 SV=1
MM1 pKa = 7.14ATLVPEE7 pKa = 4.25MEE9 pKa = 3.88NWACINGCNDD19 pKa = 4.24LGNFDD24 pKa = 4.72QSQIDD29 pKa = 3.9CALLMSLLDD38 pKa = 4.17DD39 pKa = 4.02SQLGVEE45 pKa = 4.93DD46 pKa = 3.43EE47 pKa = 4.71HH48 pKa = 8.58DD49 pKa = 3.88NKK51 pKa = 10.81RR52 pKa = 11.84LTSVIRR58 pKa = 11.84SLEE61 pKa = 3.91AEE63 pKa = 4.03IDD65 pKa = 3.74DD66 pKa = 3.63QQVINGHH73 pKa = 6.81DD74 pKa = 3.73SFQDD78 pKa = 3.41SQGRR82 pKa = 11.84NSGEE86 pKa = 3.85DD87 pKa = 3.05WQLWEE92 pKa = 4.4DD93 pKa = 3.71SQYY96 pKa = 11.49KK97 pKa = 9.91SQDD100 pKa = 3.31FLEE103 pKa = 4.86SEE105 pKa = 4.28GLDD108 pKa = 4.12LNWMALEE115 pKa = 4.09MEE117 pKa = 4.82IAPSSTSDD125 pKa = 5.77DD126 pKa = 3.28MNFWYY131 pKa = 10.21NHH133 pKa = 5.29QVHH136 pKa = 7.04DD137 pKa = 3.8MCKK140 pKa = 8.41YY141 pKa = 9.62GYY143 pKa = 10.53AAFSLEE149 pKa = 4.3EE150 pKa = 4.09NDD152 pKa = 3.74HH153 pKa = 6.19NSWGQEE159 pKa = 3.96QNSSALTMQGG169 pKa = 2.9

Molecular weight: 19.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A314L286|A0A314L286_NICAT Uncharacterized protein OS=Nicotiana attenuata OX=49451 GN=A4A49_04192 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.58GRR4 pKa = 11.84GFRR7 pKa = 11.84LGRR10 pKa = 11.84RR11 pKa = 11.84LVRR14 pKa = 11.84VFSCFIRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84TNGRR27 pKa = 11.84VGDD30 pKa = 3.7KK31 pKa = 10.32RR32 pKa = 11.84LRR34 pKa = 11.84SQGCGSRR41 pKa = 11.84AISKK45 pKa = 10.14LSKK48 pKa = 10.18CLRR51 pKa = 11.84LLKK54 pKa = 10.52HH55 pKa = 5.54GAKK58 pKa = 10.1GICFAKK64 pKa = 10.3PNSGYY69 pKa = 10.19FRR71 pKa = 11.84VGQEE75 pKa = 4.26PIDD78 pKa = 4.14PKK80 pKa = 10.74QVSVPKK86 pKa = 10.36GHH88 pKa = 6.7LAVYY92 pKa = 9.99VGEE95 pKa = 5.41KK96 pKa = 9.61IDD98 pKa = 3.6DD99 pKa = 3.83TCRR102 pKa = 11.84VVVPVIYY109 pKa = 10.43FNHH112 pKa = 7.06PLFADD117 pKa = 4.33LLRR120 pKa = 11.84EE121 pKa = 4.01AEE123 pKa = 3.89MTYY126 pKa = 10.79GYY128 pKa = 9.8NHH130 pKa = 6.8SGGIQIPCRR139 pKa = 11.84ISEE142 pKa = 4.2FEE144 pKa = 4.09NVKK147 pKa = 10.59SRR149 pKa = 11.84IAATGGGGNCRR160 pKa = 11.84RR161 pKa = 4.05

Molecular weight: 17.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32839

1

32840

12261239

45

5431

373.4

41.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.627 ± 0.013

1.843 ± 0.007

5.301 ± 0.01

6.561 ± 0.016

4.203 ± 0.009

6.324 ± 0.013

2.342 ± 0.007

5.507 ± 0.011

6.424 ± 0.013

9.477 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.006

4.749 ± 0.01

4.84 ± 0.014

3.806 ± 0.01

5.093 ± 0.011

8.864 ± 0.017

4.994 ± 0.008

6.489 ± 0.011

1.253 ± 0.005

2.869 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski