Amino acid dipepetide frequency for Nicotiana attenuata (Coyote tobacco)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.198AlaAla: 6.198 ± 0.036	1.172AlaCys: 1.172 ± 0.012	3.153AlaAsp: 3.153 ± 0.018	4.279AlaGlu: 4.279 ± 0.024	2.651AlaPhe: 2.651 ± 0.016	3.998AlaGly: 3.998 ± 0.019	1.258AlaHis: 1.258 ± 0.01	3.855AlaIle: 3.855 ± 0.021	4.099AlaLys: 4.099 ± 0.021	6.326AlaLeu: 6.326 ± 0.027	1.741AlaMet: 1.741 ± 0.011	2.779AlaAsn: 2.779 ± 0.015	2.771AlaPro: 2.771 ± 0.018	2.156AlaGln: 2.156 ± 0.016	3.224AlaArg: 3.224 ± 0.019	5.738AlaSer: 5.738 ± 0.025	3.712AlaThr: 3.712 ± 0.02	4.659AlaVal: 4.659 ± 0.024	0.712AlaTrp: 0.712 ± 0.008	1.789AlaTyr: 1.789 ± 0.013	0.001AlaXaa: 0.001 ± 0.0
Cys	0.976CysAla: 0.976 ± 0.009	0.53CysCys: 0.53 ± 0.009	0.895CysAsp: 0.895 ± 0.009	0.882CysGlu: 0.882 ± 0.009	0.884CysPhe: 0.884 ± 0.009	1.354CysGly: 1.354 ± 0.013	0.451CysHis: 0.451 ± 0.006	1.011CysIle: 1.011 ± 0.009	1.204CysLys: 1.204 ± 0.011	1.791CysLeu: 1.791 ± 0.014	0.433CysMet: 0.433 ± 0.006	0.899CysAsn: 0.899 ± 0.01	0.937CysPro: 0.937 ± 0.01	0.626CysGln: 0.626 ± 0.008	1.011CysArg: 1.011 ± 0.011	1.808CysSer: 1.808 ± 0.014	0.895CysThr: 0.895 ± 0.008	1.019CysVal: 1.019 ± 0.01	0.246CysTrp: 0.246 ± 0.005	0.577CysTyr: 0.577 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	3.364AspAla: 3.364 ± 0.018	0.954AspCys: 0.954 ± 0.009	3.6AspAsp: 3.6 ± 0.025	4.105AspGlu: 4.105 ± 0.021	2.389AspPhe: 2.389 ± 0.016	3.554AspGly: 3.554 ± 0.02	1.245AspHis: 1.245 ± 0.011	3.209AspIle: 3.209 ± 0.016	2.925AspLys: 2.925 ± 0.017	4.959AspLeu: 4.959 ± 0.023	1.344AspMet: 1.344 ± 0.01	2.271AspAsn: 2.271 ± 0.014	2.507AspPro: 2.507 ± 0.017	1.845AspGln: 1.845 ± 0.015	2.286AspArg: 2.286 ± 0.017	4.245AspSer: 4.245 ± 0.02	2.311AspThr: 2.311 ± 0.014	3.61AspVal: 3.61 ± 0.018	0.686AspTrp: 0.686 ± 0.009	1.605AspTyr: 1.605 ± 0.011	0.0AspXaa: 0.0 ± 0.0
Glu	4.653GluAla: 4.653 ± 0.024	0.928GluCys: 0.928 ± 0.009	3.994GluAsp: 3.994 ± 0.02	6.43GluGlu: 6.43 ± 0.039	2.386GluPhe: 2.386 ± 0.015	3.595GluGly: 3.595 ± 0.019	1.281GluHis: 1.281 ± 0.011	3.941GluIle: 3.941 ± 0.021	5.072GluLys: 5.072 ± 0.027	5.979GluLeu: 5.979 ± 0.027	1.848GluMet: 1.848 ± 0.015	3.43GluAsn: 3.43 ± 0.017	2.137GluPro: 2.137 ± 0.015	2.334GluGln: 2.334 ± 0.016	3.252GluArg: 3.252 ± 0.023	4.607GluSer: 4.607 ± 0.024	3.016GluThr: 3.016 ± 0.019	4.28GluVal: 4.28 ± 0.019	0.715GluTrp: 0.715 ± 0.007	1.73GluTyr: 1.73 ± 0.011	0.0GluXaa: 0.0 ± 0.0
Phe	2.504PheAla: 2.504 ± 0.015	0.912PheCys: 0.912 ± 0.008	2.41PheAsp: 2.41 ± 0.015	2.351PheGlu: 2.351 ± 0.013	1.963PhePhe: 1.963 ± 0.016	2.979PheGly: 2.979 ± 0.018	1.105PheHis: 1.105 ± 0.009	2.151PheIle: 2.151 ± 0.016	2.207PheLys: 2.207 ± 0.014	4.261PheLeu: 4.261 ± 0.02	0.957PheMet: 0.957 ± 0.009	1.862PheAsn: 1.862 ± 0.014	2.065PhePro: 2.065 ± 0.015	1.61PheGln: 1.61 ± 0.012	1.988PheArg: 1.988 ± 0.015	4.081PheSer: 4.081 ± 0.019	2.038PheThr: 2.038 ± 0.012	2.715PheVal: 2.715 ± 0.016	0.55PheTrp: 0.55 ± 0.007	1.317PheTyr: 1.317 ± 0.01	0.001PheXaa: 0.001 ± 0.0
Gly	3.765GlyAla: 3.765 ± 0.019	1.239GlyCys: 1.239 ± 0.01	3.296GlyAsp: 3.296 ± 0.017	3.605GlyGlu: 3.605 ± 0.018	2.963GlyPhe: 2.963 ± 0.018	4.953GlyGly: 4.953 ± 0.034	1.499GlyHis: 1.499 ± 0.013	3.622GlyIle: 3.622 ± 0.022	4.248GlyLys: 4.248 ± 0.021	5.532GlyLeu: 5.532 ± 0.026	1.48GlyMet: 1.48 ± 0.013	3.243GlyAsn: 3.243 ± 0.018	2.342GlyPro: 2.342 ± 0.016	2.122GlyGln: 2.122 ± 0.015	3.376GlyArg: 3.376 ± 0.018	5.7GlySer: 5.7 ± 0.024	3.214GlyThr: 3.214 ± 0.019	4.124GlyVal: 4.124 ± 0.018	0.835GlyTrp: 0.835 ± 0.009	2.086GlyTyr: 2.086 ± 0.019	0.001GlyXaa: 0.001 ± 0.0
His	1.358HisAla: 1.358 ± 0.011	0.513HisCys: 0.513 ± 0.006	1.144HisAsp: 1.144 ± 0.01	1.279HisGlu: 1.279 ± 0.01	1.108HisPhe: 1.108 ± 0.009	1.594HisGly: 1.594 ± 0.012	0.869HisHis: 0.869 ± 0.011	1.291HisIle: 1.291 ± 0.012	1.239HisLys: 1.239 ± 0.011	2.338HisLeu: 2.338 ± 0.015	0.572HisMet: 0.572 ± 0.006	1.049HisAsn: 1.049 ± 0.01	1.282HisPro: 1.282 ± 0.011	1.008HisGln: 1.008 ± 0.01	1.247HisArg: 1.247 ± 0.01	1.947HisSer: 1.947 ± 0.015	1.002HisThr: 1.002 ± 0.01	1.546HisVal: 1.546 ± 0.011	0.307HisTrp: 0.307 ± 0.006	0.725HisTyr: 0.725 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.626IleAla: 3.626 ± 0.021	1.16IleCys: 1.16 ± 0.01	3.02IleAsp: 3.02 ± 0.017	3.366IleGlu: 3.366 ± 0.017	2.422IlePhe: 2.422 ± 0.016	3.496IleGly: 3.496 ± 0.019	1.336IleHis: 1.336 ± 0.012	2.961IleIle: 2.961 ± 0.017	3.134IleLys: 3.134 ± 0.017	5.367IleLeu: 5.367 ± 0.021	1.222IleMet: 1.222 ± 0.011	2.376IleAsn: 2.376 ± 0.015	3.032IlePro: 3.032 ± 0.02	2.141IleGln: 2.141 ± 0.015	2.622IleArg: 2.622 ± 0.015	5.118IleSer: 5.118 ± 0.025	2.743IleThr: 2.743 ± 0.015	3.603IleVal: 3.603 ± 0.019	0.752IleTrp: 0.752 ± 0.009	1.57IleTyr: 1.57 ± 0.012	0.001IleXaa: 0.001 ± 0.0
Lys	4.139LysAla: 4.139 ± 0.021	1.042LysCys: 1.042 ± 0.01	3.435LysAsp: 3.435 ± 0.018	5.047LysGlu: 5.047 ± 0.027	2.376LysPhe: 2.376 ± 0.013	3.783LysGly: 3.783 ± 0.02	1.387LysHis: 1.387 ± 0.01	3.499LysIle: 3.499 ± 0.02	5.219LysLys: 5.219 ± 0.029	6.198LysLeu: 6.198 ± 0.027	1.606LysMet: 1.606 ± 0.012	3.002LysAsn: 3.002 ± 0.014	2.793LysPro: 2.793 ± 0.017	2.496LysGln: 2.496 ± 0.017	3.624LysArg: 3.624 ± 0.02	4.925LysSer: 4.925 ± 0.021	2.973LysThr: 2.973 ± 0.014	4.044LysVal: 4.044 ± 0.021	0.829LysTrp: 0.829 ± 0.009	1.824LysTyr: 1.824 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	6.295LeuAla: 6.295 ± 0.027	1.791LeuCys: 1.791 ± 0.014	4.959LeuAsp: 4.959 ± 0.022	6.216LeuGlu: 6.216 ± 0.028	3.811LeuPhe: 3.811 ± 0.022	5.555LeuGly: 5.555 ± 0.027	2.492LeuHis: 2.492 ± 0.015	4.743LeuIle: 4.743 ± 0.024	6.348LeuLys: 6.348 ± 0.027	9.586LeuLeu: 9.586 ± 0.044	2.17LeuMet: 2.17 ± 0.015	4.082LeuAsn: 4.082 ± 0.02	5.029LeuPro: 5.029 ± 0.022	4.196LeuGln: 4.196 ± 0.026	5.037LeuArg: 5.037 ± 0.025	8.238LeuSer: 8.238 ± 0.032	4.37LeuThr: 4.37 ± 0.023	6.201LeuVal: 6.201 ± 0.026	1.132LeuTrp: 1.132 ± 0.011	2.517LeuTyr: 2.517 ± 0.015	0.001LeuXaa: 0.001 ± 0.0
Met	2.113MetAla: 2.113 ± 0.013	0.327MetCys: 0.327 ± 0.005	1.449MetAsp: 1.449 ± 0.012	1.971MetGlu: 1.971 ± 0.015	0.816MetPhe: 0.816 ± 0.01	1.538MetGly: 1.538 ± 0.013	0.545MetHis: 0.545 ± 0.008	1.234MetIle: 1.234 ± 0.01	1.723MetLys: 1.723 ± 0.012	2.17MetLeu: 2.17 ± 0.015	0.676MetMet: 0.676 ± 0.008	1.092MetAsn: 1.092 ± 0.01	1.057MetPro: 1.057 ± 0.009	1.018MetGln: 1.018 ± 0.009	1.182MetArg: 1.182 ± 0.01	1.797MetSer: 1.797 ± 0.012	1.105MetThr: 1.105 ± 0.01	1.651MetVal: 1.651 ± 0.013	0.269MetTrp: 0.269 ± 0.005	0.608MetTyr: 0.608 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.79AsnAla: 2.79 ± 0.015	0.902AsnCys: 0.902 ± 0.009	2.229AsnAsp: 2.229 ± 0.014	2.787AsnGlu: 2.787 ± 0.016	2.139AsnPhe: 2.139 ± 0.015	3.413AsnGly: 3.413 ± 0.021	1.148AsnHis: 1.148 ± 0.012	2.758AsnIle: 2.758 ± 0.016	2.73AsnLys: 2.73 ± 0.018	4.656AsnLeu: 4.656 ± 0.028	1.232AsnMet: 1.232 ± 0.01	2.731AsnAsn: 2.731 ± 0.029	2.454AsnPro: 2.454 ± 0.016	1.89AsnGln: 1.89 ± 0.012	2.084AsnArg: 2.084 ± 0.015	4.294AsnSer: 4.294 ± 0.024	2.201AsnThr: 2.201 ± 0.015	3.011AsnVal: 3.011 ± 0.016	0.599AsnTrp: 0.599 ± 0.008	1.443AsnTyr: 1.443 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	2.983ProAla: 2.983 ± 0.02	0.759ProCys: 0.759 ± 0.007	2.348ProAsp: 2.348 ± 0.015	3.054ProGlu: 3.054 ± 0.019	2.012ProPhe: 2.012 ± 0.013	2.561ProGly: 2.561 ± 0.017	1.068ProHis: 1.068 ± 0.01	2.494ProIle: 2.494 ± 0.014	2.883ProLys: 2.883 ± 0.019	4.257ProLeu: 4.257 ± 0.018	0.979ProMet: 0.979 ± 0.01	2.442ProAsn: 2.442 ± 0.017	3.772ProPro: 3.772 ± 0.054	1.839ProGln: 1.839 ± 0.017	2.28ProArg: 2.28 ± 0.015	4.945ProSer: 4.945 ± 0.027	2.779ProThr: 2.779 ± 0.017	3.016ProVal: 3.016 ± 0.019	0.585ProTrp: 0.585 ± 0.007	1.34ProTyr: 1.34 ± 0.012	0.0ProXaa: 0.0 ± 0.0
Gln	2.392GlnAla: 2.392 ± 0.018	0.59GlnCys: 0.59 ± 0.007	1.73GlnAsp: 1.73 ± 0.013	2.539GlnGlu: 2.539 ± 0.017	1.432GlnPhe: 1.432 ± 0.01	2.128GlnGly: 2.128 ± 0.014	0.969GlnHis: 0.969 ± 0.01	2.095GlnIle: 2.095 ± 0.015	2.549GlnLys: 2.549 ± 0.017	3.774GlnLeu: 3.774 ± 0.022	1.004GlnMet: 1.004 ± 0.01	2.026GlnAsn: 2.026 ± 0.016	1.795GlnPro: 1.795 ± 0.014	2.343GlnGln: 2.343 ± 0.03	2.096GlnArg: 2.096 ± 0.014	2.94GlnSer: 2.94 ± 0.02	1.759GlnThr: 1.759 ± 0.013	2.425GlnVal: 2.425 ± 0.014	0.471GlnTrp: 0.471 ± 0.006	1.002GlnTyr: 1.002 ± 0.008	0.0GlnXaa: 0.0 ± 0.0
Arg	3.082ArgAla: 3.082 ± 0.017	0.92ArgCys: 0.92 ± 0.009	2.561ArgAsp: 2.561 ± 0.015	3.215ArgGlu: 3.215 ± 0.02	2.117ArgPhe: 2.117 ± 0.014	3.058ArgGly: 3.058 ± 0.018	1.208ArgHis: 1.208 ± 0.01	2.816ArgIle: 2.816 ± 0.016	3.852ArgLys: 3.852 ± 0.019	4.701ArgLeu: 4.701 ± 0.024	1.262ArgMet: 1.262 ± 0.01	2.506ArgAsn: 2.506 ± 0.014	2.237ArgPro: 2.237 ± 0.016	1.828ArgGln: 1.828 ± 0.016	3.756ArgArg: 3.756 ± 0.024	4.146ArgSer: 4.146 ± 0.024	2.374ArgThr: 2.374 ± 0.014	3.15ArgVal: 3.15 ± 0.02	0.701ArgTrp: 0.701 ± 0.008	1.441ArgTyr: 1.441 ± 0.011	0.0ArgXaa: 0.0 ± 0.0
Ser	5.338SerAla: 5.338 ± 0.024	1.744SerCys: 1.744 ± 0.014	4.42SerAsp: 4.42 ± 0.019	4.754SerGlu: 4.754 ± 0.022	3.913SerPhe: 3.913 ± 0.019	5.661SerGly: 5.661 ± 0.024	1.98SerHis: 1.98 ± 0.014	4.695SerIle: 4.695 ± 0.023	5.171SerLys: 5.171 ± 0.024	8.231SerLeu: 8.231 ± 0.03	2.045SerMet: 2.045 ± 0.013	4.31SerAsn: 4.31 ± 0.019	4.523SerPro: 4.523 ± 0.035	3.132SerGln: 3.132 ± 0.02	4.311SerArg: 4.311 ± 0.018	10.824SerSer: 10.824 ± 0.053	4.947SerThr: 4.947 ± 0.024	5.131SerVal: 5.131 ± 0.023	1.135SerTrp: 1.135 ± 0.011	2.377SerTyr: 2.377 ± 0.014	0.001SerXaa: 0.001 ± 0.0
Thr	3.41ThrAla: 3.41 ± 0.019	0.971ThrCys: 0.971 ± 0.009	2.369ThrAsp: 2.369 ± 0.014	2.847ThrGlu: 2.847 ± 0.017	2.085ThrPhe: 2.085 ± 0.013	3.257ThrGly: 3.257 ± 0.019	1.05ThrHis: 1.05 ± 0.011	2.921ThrIle: 2.921 ± 0.016	2.894ThrLys: 2.894 ± 0.018	4.581ThrLeu: 4.581 ± 0.021	1.166ThrMet: 1.166 ± 0.01	2.322ThrAsn: 2.322 ± 0.014	2.696ThrPro: 2.696 ± 0.018	1.644ThrGln: 1.644 ± 0.012	2.356ThrArg: 2.356 ± 0.014	4.778ThrSer: 4.778 ± 0.023	3.228ThrThr: 3.228 ± 0.021	3.303ThrVal: 3.303 ± 0.016	0.636ThrTrp: 0.636 ± 0.008	1.422ThrTyr: 1.422 ± 0.012	0.0ThrXaa: 0.0 ± 0.0
Val	4.737ValAla: 4.737 ± 0.023	1.092ValCys: 1.092 ± 0.009	3.734ValAsp: 3.734 ± 0.017	4.546ValGlu: 4.546 ± 0.024	2.647ValPhe: 2.647 ± 0.015	3.978ValGly: 3.978 ± 0.021	1.505ValHis: 1.505 ± 0.012	3.509ValIle: 3.509 ± 0.017	4.127ValLys: 4.127 ± 0.02	6.098ValLeu: 6.098 ± 0.027	1.535ValMet: 1.535 ± 0.014	2.888ValAsn: 2.888 ± 0.018	3.206ValPro: 3.206 ± 0.018	2.4ValGln: 2.4 ± 0.014	2.953ValArg: 2.953 ± 0.019	5.19ValSer: 5.19 ± 0.02	3.297ValThr: 3.297 ± 0.018	4.812ValVal: 4.812 ± 0.021	0.731ValTrp: 0.731 ± 0.008	1.902ValTyr: 1.902 ± 0.013	0.0ValXaa: 0.0 ± 0.0
Trp	0.744TrpAla: 0.744 ± 0.008	0.235TrpCys: 0.235 ± 0.005	0.688TrpAsp: 0.688 ± 0.007	0.737TrpGlu: 0.737 ± 0.008	0.545TrpPhe: 0.545 ± 0.007	0.696TrpGly: 0.696 ± 0.008	0.287TrpHis: 0.287 ± 0.005	0.751TrpIle: 0.751 ± 0.007	0.954TrpLys: 0.954 ± 0.008	1.198TrpLeu: 1.198 ± 0.012	0.334TrpMet: 0.334 ± 0.006	0.719TrpAsn: 0.719 ± 0.008	0.469TrpPro: 0.469 ± 0.006	0.44TrpGln: 0.44 ± 0.006	0.78TrpArg: 0.78 ± 0.009	0.963TrpSer: 0.963 ± 0.009	0.646TrpThr: 0.646 ± 0.008	0.766TrpVal: 0.766 ± 0.009	0.234TrpTrp: 0.234 ± 0.005	0.348TrpTyr: 0.348 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.801TyrAla: 1.801 ± 0.013	0.648TyrCys: 0.648 ± 0.008	1.577TyrAsp: 1.577 ± 0.014	1.597TyrGlu: 1.597 ± 0.011	1.368TyrPhe: 1.368 ± 0.011	2.093TyrGly: 2.093 ± 0.016	0.734TyrHis: 0.734 ± 0.008	1.51TyrIle: 1.51 ± 0.012	1.666TyrLys: 1.666 ± 0.014	2.766TyrLeu: 2.766 ± 0.018	0.735TyrMet: 0.735 ± 0.008	1.46TyrAsn: 1.46 ± 0.012	1.291TyrPro: 1.291 ± 0.01	0.992TyrGln: 0.992 ± 0.009	1.467TyrArg: 1.467 ± 0.011	2.357TyrSer: 2.357 ± 0.014	1.328TyrThr: 1.328 ± 0.011	1.822TyrVal: 1.822 ± 0.012	0.407TyrTrp: 0.407 ± 0.006	1.067TyrTyr: 1.067 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 32840 proteins (12261239 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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