Gordonia phage OneUp

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Oneupvirus; Gordonia virus OneUp

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 163 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DHA2|A0A160DHA2_9CAUD Uncharacterized protein OS=Gordonia phage OneUp OX=1838074 GN=11 PE=4 SV=1
MM1 pKa = 7.31TWSCYY6 pKa = 8.8DD7 pKa = 3.45TLEE10 pKa = 4.65LDD12 pKa = 3.76NSVHH16 pKa = 6.65VIPDD20 pKa = 3.66LDD22 pKa = 5.16IIEE25 pKa = 5.23HH26 pKa = 6.46DD27 pKa = 3.62QSDD30 pKa = 4.03DD31 pKa = 4.08CVCGPAVEE39 pKa = 4.47PVPLATGGMSWLVTHH54 pKa = 6.85HH55 pKa = 6.63SLDD58 pKa = 3.39GRR60 pKa = 11.84EE61 pKa = 3.93ADD63 pKa = 3.71EE64 pKa = 4.18

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DHC8|A0A160DHC8_9CAUD Tail assembly chaperone OS=Gordonia phage OneUp OX=1838074 GN=39 PE=4 SV=1
MM1 pKa = 7.82SDD3 pKa = 3.81DD4 pKa = 3.79RR5 pKa = 11.84FANRR9 pKa = 11.84PLPHH13 pKa = 6.78GDD15 pKa = 2.69FWGPSGPADD24 pKa = 3.27PRR26 pKa = 11.84VTARR30 pKa = 11.84NSVRR34 pKa = 11.84TSRR37 pKa = 11.84QQYY40 pKa = 9.92RR41 pKa = 11.84EE42 pKa = 4.03GAQPWSNLTDD52 pKa = 3.46KK53 pKa = 11.01EE54 pKa = 3.94RR55 pKa = 11.84GYY57 pKa = 11.04LYY59 pKa = 10.74DD60 pKa = 3.89RR61 pKa = 11.84GEE63 pKa = 4.27VVEE66 pKa = 4.5TPGGYY71 pKa = 8.02EE72 pKa = 3.63VRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84DD77 pKa = 3.86LSSMGLVEE85 pKa = 4.27EE86 pKa = 4.52FGEE89 pKa = 4.3VRR91 pKa = 11.84RR92 pKa = 11.84KK93 pKa = 9.55KK94 pKa = 10.35VRR96 pKa = 11.84AGTGTSLPKK105 pKa = 9.43TRR107 pKa = 11.84KK108 pKa = 7.66STVRR112 pKa = 11.84QVNRR116 pKa = 11.84KK117 pKa = 9.32SVDD120 pKa = 2.95KK121 pKa = 11.18SRR123 pKa = 11.84AAANRR128 pKa = 11.84ARR130 pKa = 11.84QVDD133 pKa = 4.57EE134 pKa = 3.85YY135 pKa = 11.65ARR137 pKa = 11.84RR138 pKa = 11.84LVNRR142 pKa = 11.84DD143 pKa = 2.98PADD146 pKa = 3.27WQRR149 pKa = 11.84WMGTNVPKK157 pKa = 10.68SLQNEE162 pKa = 4.22VVMATIAMGTTVNAA176 pKa = 4.27

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

163

0

163

28909

37

2836

177.4

19.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.654 ± 0.274

0.969 ± 0.125

7.115 ± 0.188

6.88 ± 0.27

2.989 ± 0.1

7.973 ± 0.326

2.186 ± 0.147

4.888 ± 0.142

4.611 ± 0.203

7.482 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.097

3.916 ± 0.134

4.58 ± 0.165

3.642 ± 0.189

6.431 ± 0.219

5.528 ± 0.177

6.223 ± 0.217

7.122 ± 0.225

2.096 ± 0.086

3.217 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski