Cordyceps militaris (strain CM01) (Caterpillar fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Cordycipitaceae; Cordyceps; Cordyceps militaris

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9651 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3JKF2|G3JKF2_CORMM Serine/threonine protein kinase putative OS=Cordyceps militaris (strain CM01) OX=983644 GN=CCM_06391 PE=4 SV=1
MM1 pKa = 7.32TPDD4 pKa = 3.19NTTLTNKK11 pKa = 8.42YY12 pKa = 5.27TTFRR16 pKa = 11.84DD17 pKa = 3.43HH18 pKa = 5.9YY19 pKa = 9.03TALTHH24 pKa = 6.59SYY26 pKa = 9.17TMSYY30 pKa = 7.82HH31 pKa = 5.86TRR33 pKa = 11.84PEE35 pKa = 4.09QNQSLTVYY43 pKa = 8.0QHH45 pKa = 6.31PQTLITSDD53 pKa = 3.68VEE55 pKa = 4.44MKK57 pKa = 10.79FSDD60 pKa = 3.75DD61 pKa = 3.84EE62 pKa = 4.59NFFSYY67 pKa = 11.11DD68 pKa = 3.48DD69 pKa = 4.25TVTCDD74 pKa = 3.09NDD76 pKa = 3.64IVMTDD81 pKa = 3.1VNTIKK86 pKa = 10.06TDD88 pKa = 3.36NANVLDD94 pKa = 4.53GDD96 pKa = 4.06ILMTGADD103 pKa = 3.62QVITEE108 pKa = 4.54VDD110 pKa = 3.78TIMTEE115 pKa = 3.96ADD117 pKa = 3.09TTMTDD122 pKa = 3.91DD123 pKa = 3.96DD124 pKa = 4.33TLMVDD129 pKa = 3.64PHH131 pKa = 7.2TIMGDD136 pKa = 3.17ADD138 pKa = 3.74YY139 pKa = 11.53LDD141 pKa = 3.75IGGNFYY147 pKa = 10.09LTFTFVAISII157 pKa = 4.18

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3JGF4|G3JGF4_CORMM Uncharacterized protein OS=Cordyceps militaris (strain CM01) OX=983644 GN=CCM_03744 PE=4 SV=1
MM1 pKa = 7.12FRR3 pKa = 11.84VLSSQGGSICNVHH16 pKa = 6.09PTRR19 pKa = 11.84LLTGPAPSSPTILLIASTRR38 pKa = 11.84FLFQFPPTPLATPSSGRR55 pKa = 11.84PPLISLVPNTTIIIRR70 pKa = 11.84ILSVGASARR79 pKa = 11.84QHH81 pKa = 5.46FNHH84 pKa = 7.38RR85 pKa = 11.84ISHH88 pKa = 6.08PRR90 pKa = 11.84NTSRR94 pKa = 11.84RR95 pKa = 11.84TSQRR99 pKa = 11.84WW100 pKa = 2.94

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9651

0

9651

4871812

50

5129

504.8

55.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.924 ± 0.027

1.299 ± 0.009

5.787 ± 0.019

5.657 ± 0.024

3.617 ± 0.015

7.01 ± 0.024

2.487 ± 0.012

4.464 ± 0.018

4.457 ± 0.021

9.009 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.009

3.37 ± 0.011

6.097 ± 0.026

4.003 ± 0.016

6.427 ± 0.022

7.855 ± 0.025

5.931 ± 0.017

6.284 ± 0.017

1.457 ± 0.009

2.64 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski