Circovirus-like genome DCCV-12

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHF0|A0A190WHF0_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-12 OX=1788440 PE=4 SV=1
MM1 pKa = 7.53PPKK4 pKa = 10.4AQFNRR9 pKa = 11.84FCQITKK15 pKa = 10.26KK16 pKa = 10.52RR17 pKa = 11.84GFCWTYY23 pKa = 10.59HH24 pKa = 7.02DD25 pKa = 4.68YY26 pKa = 11.56DD27 pKa = 4.73DD28 pKa = 6.15EE29 pKa = 4.82IVAHH33 pKa = 6.61IKK35 pKa = 10.92GLFDD39 pKa = 5.37RR40 pKa = 11.84PNGWVKK46 pKa = 10.57RR47 pKa = 11.84LVFNYY52 pKa = 7.44EE53 pKa = 3.54ICPTTGRR60 pKa = 11.84RR61 pKa = 11.84HH62 pKa = 5.42LQGCLYY68 pKa = 10.25MRR70 pKa = 11.84DD71 pKa = 3.49NQTWPKK77 pKa = 10.14LKK79 pKa = 10.61ADD81 pKa = 3.41IGLEE85 pKa = 4.18KK86 pKa = 10.33IHH88 pKa = 7.17LEE90 pKa = 3.72LAQNVEE96 pKa = 4.71ALDD99 pKa = 4.26KK100 pKa = 10.78YY101 pKa = 9.03CQKK104 pKa = 10.87SEE106 pKa = 4.19TRR108 pKa = 11.84DD109 pKa = 3.36PAHH112 pKa = 7.13PEE114 pKa = 3.99GVVLGVAPLTDD125 pKa = 3.34EE126 pKa = 5.21AKK128 pKa = 9.22GQKK131 pKa = 10.11GEE133 pKa = 4.06EE134 pKa = 4.3YY135 pKa = 10.63YY136 pKa = 10.81EE137 pKa = 4.38RR138 pKa = 11.84NILACMNGQQMDD150 pKa = 3.56ASAEE154 pKa = 3.97FNLRR158 pKa = 11.84NFEE161 pKa = 4.15YY162 pKa = 10.54AAEE165 pKa = 3.9ARR167 pKa = 11.84KK168 pKa = 9.6RR169 pKa = 11.84KK170 pKa = 9.86RR171 pKa = 11.84EE172 pKa = 3.92LASLDD177 pKa = 3.5EE178 pKa = 5.91LSFEE182 pKa = 4.01WHH184 pKa = 6.01YY185 pKa = 11.75GEE187 pKa = 4.75PFSGKK192 pKa = 6.61THH194 pKa = 5.96YY195 pKa = 10.01CRR197 pKa = 11.84KK198 pKa = 9.73IPGAFKK204 pKa = 10.13WNSKK208 pKa = 10.48AGWNNYY214 pKa = 8.25NDD216 pKa = 4.18EE217 pKa = 4.43EE218 pKa = 4.81VVICDD223 pKa = 4.01DD224 pKa = 3.29VDD226 pKa = 3.35EE227 pKa = 4.54RR228 pKa = 11.84TVPEE232 pKa = 3.86QQEE235 pKa = 4.01IKK237 pKa = 8.79TWCDD241 pKa = 3.15LDD243 pKa = 3.87PFQVKK248 pKa = 9.35VNYY251 pKa = 9.43KK252 pKa = 9.77VLNIRR257 pKa = 11.84PKK259 pKa = 10.71RR260 pKa = 11.84FIFTSNEE267 pKa = 4.0SIADD271 pKa = 3.68CFPRR275 pKa = 11.84AKK277 pKa = 10.05PIHH280 pKa = 6.25LKK282 pKa = 10.44AIEE285 pKa = 4.01RR286 pKa = 11.84RR287 pKa = 11.84FKK289 pKa = 10.78VYY291 pKa = 10.23YY292 pKa = 9.83YY293 pKa = 9.07PAAYY297 pKa = 9.77GEE299 pKa = 4.66PGWVDD304 pKa = 3.39PTAIPQKK311 pKa = 10.94YY312 pKa = 9.36LFTDD316 pKa = 3.66EE317 pKa = 5.52EE318 pKa = 4.1MDD320 pKa = 3.48AQLSVV325 pKa = 3.14

Molecular weight: 38.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHM0|A0A190WHM0_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-12 OX=1788440 PE=4 SV=1
MM1 pKa = 7.38ASKK4 pKa = 7.96WTPARR9 pKa = 11.84SSIYY13 pKa = 9.0GTSSTLPRR21 pKa = 11.84LANANANLLHH31 pKa = 6.58SMSFHH36 pKa = 6.9SSGITASLLAARR48 pKa = 11.84LIIAVRR54 pKa = 11.84SQVPSNGIRR63 pKa = 11.84RR64 pKa = 11.84LVGTTTTMRR73 pKa = 11.84RR74 pKa = 11.84SSYY77 pKa = 11.29VMTSMNVLFPSNRR90 pKa = 11.84RR91 pKa = 11.84LRR93 pKa = 11.84PGVISIPSRR102 pKa = 11.84SRR104 pKa = 11.84LITRR108 pKa = 11.84CC109 pKa = 3.32

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

727

109

325

242.3

27.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.978 ± 0.362

1.513 ± 0.962

4.677 ± 1.542

4.952 ± 2.334

4.539 ± 0.826

5.365 ± 0.298

1.788 ± 0.444

5.502 ± 0.58

7.153 ± 1.907

7.153 ± 0.899

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.795

5.915 ± 0.705

6.052 ± 0.26

3.301 ± 0.812

7.703 ± 1.61

5.777 ± 3.586

5.365 ± 1.235

6.327 ± 0.85

1.651 ± 0.492

4.539 ± 0.832
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski