Actinomadura sp. WAC 06369

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura; unclassified Actinomadura

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429FJA4|A0A429FJA4_9ACTN Uncharacterized protein OS=Actinomadura sp. WAC 06369 OX=2203193 GN=DMH08_20880 PE=4 SV=1
MM1 pKa = 7.32VFGLLVVVVLVVVLVVLGLRR21 pKa = 11.84ASKK24 pKa = 10.56AGRR27 pKa = 11.84DD28 pKa = 3.64DD29 pKa = 5.11DD30 pKa = 5.54DD31 pKa = 4.14WMDD34 pKa = 4.8DD35 pKa = 3.64EE36 pKa = 4.89QPQPRR41 pKa = 11.84GRR43 pKa = 11.84RR44 pKa = 11.84GAAQQDD50 pKa = 4.26EE51 pKa = 4.87MNGPDD56 pKa = 4.51DD57 pKa = 3.92YY58 pKa = 11.65GYY60 pKa = 11.22DD61 pKa = 3.61DD62 pKa = 4.77GGYY65 pKa = 9.71GAPAPGPDD73 pKa = 3.32RR74 pKa = 11.84RR75 pKa = 11.84VAGAGAGAGGPLAAPAGQQGPPAPPPSGQASDD107 pKa = 3.99EE108 pKa = 4.36MEE110 pKa = 5.66DD111 pKa = 3.38DD112 pKa = 5.39DD113 pKa = 4.41YY114 pKa = 11.55WATITFDD121 pKa = 3.31KK122 pKa = 11.02PKK124 pKa = 10.51FPWQHH129 pKa = 4.71NQEE132 pKa = 4.2GQRR135 pKa = 11.84PDD137 pKa = 3.45GDD139 pKa = 3.83HH140 pKa = 7.05AADD143 pKa = 4.29PLHH146 pKa = 6.66AQGGDD151 pKa = 3.3DD152 pKa = 5.36GYY154 pKa = 11.53GDD156 pKa = 3.6QYY158 pKa = 11.89GDD160 pKa = 3.34QQYY163 pKa = 11.61GDD165 pKa = 3.67QQYY168 pKa = 11.58GDD170 pKa = 3.6QQYY173 pKa = 10.81GDD175 pKa = 3.98QYY177 pKa = 11.38ADD179 pKa = 3.14QYY181 pKa = 11.91GDD183 pKa = 3.23QQYY186 pKa = 11.62ADD188 pKa = 3.5QQQYY192 pKa = 9.92GDD194 pKa = 3.7QYY196 pKa = 10.1GDD198 pKa = 3.18QYY200 pKa = 11.85AEE202 pKa = 4.38QDD204 pKa = 3.83PQVPDD209 pKa = 3.1HH210 pKa = 6.76GMNQTVTLGQEE221 pKa = 4.09GGAFGAPGGPGQGPPNQGPPGPGGPGQTGGQPAPTGPQQAPGGPGGFQGGSPFGGGDD278 pKa = 3.49PGATALDD285 pKa = 5.26PIPADD290 pKa = 3.51LAGGPGMQGGPGAHH304 pKa = 6.74APFAPGGPDD313 pKa = 2.85QHH315 pKa = 8.63VYY317 pKa = 10.65GGQDD321 pKa = 3.13AQPAYY326 pKa = 10.42GSAEE330 pKa = 4.03PPFGGPEE337 pKa = 3.73QQPPYY342 pKa = 10.64GGDD345 pKa = 3.14RR346 pKa = 11.84QQPYY350 pKa = 10.7AEE352 pKa = 4.3PQQPGMPPEE361 pKa = 4.18PAPYY365 pKa = 10.17GLPEE369 pKa = 3.85QPQPYY374 pKa = 10.11GGDD377 pKa = 3.52PLASGGRR384 pKa = 11.84PTPASPPPGRR394 pKa = 11.84PDD396 pKa = 3.47AFDD399 pKa = 3.72MPLGASGPGSTGPNQGPGSTGPNQGPPAPPPSDD432 pKa = 3.69PLGLPLGRR440 pKa = 11.84TDD442 pKa = 4.13EE443 pKa = 4.37PAAPGGSGPDD453 pKa = 3.5TDD455 pKa = 3.54NHH457 pKa = 6.43KK458 pKa = 10.98LPTVDD463 pKa = 5.16EE464 pKa = 4.24LLQRR468 pKa = 11.84IQSDD472 pKa = 3.97RR473 pKa = 11.84QRR475 pKa = 11.84SSGPPSGDD483 pKa = 3.01SGGSSYY489 pKa = 11.15GGPLGDD495 pKa = 4.64PLSDD499 pKa = 3.72PLATGSYY506 pKa = 10.04GGSTGAGPGTGQGSSTGPWSPGGGLGNSGGYY537 pKa = 7.93PQGGQSEE544 pKa = 5.69GYY546 pKa = 8.35PASPSYY552 pKa = 11.58GEE554 pKa = 4.15GNRR557 pKa = 11.84YY558 pKa = 9.8DD559 pKa = 4.64DD560 pKa = 4.65PLSGGRR566 pKa = 11.84DD567 pKa = 3.37AYY569 pKa = 10.62GGEE572 pKa = 4.34SGSGGAGAYY581 pKa = 10.38GDD583 pKa = 4.49FSGSSYY589 pKa = 11.05SGNDD593 pKa = 3.77PLSAPHH599 pKa = 7.4DD600 pKa = 4.5PGAQSGDD607 pKa = 3.75PNSTQAYY614 pKa = 8.96DD615 pKa = 3.61PGFGRR620 pKa = 11.84QGGYY624 pKa = 10.04QGDD627 pKa = 3.59DD628 pKa = 3.34WEE630 pKa = 4.16NHH632 pKa = 4.55RR633 pKa = 11.84DD634 pKa = 3.59YY635 pKa = 11.29RR636 pKa = 11.84RR637 pKa = 3.16

Molecular weight: 64.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A429FF43|A0A429FF43_9ACTN CoA transferase OS=Actinomadura sp. WAC 06369 OX=2203193 GN=DMH08_21255 PE=4 SV=1
SS1 pKa = 7.08RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84CGRR7 pKa = 11.84GGTARR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84GRR16 pKa = 11.84RR17 pKa = 11.84GRR19 pKa = 11.84GARR22 pKa = 11.84RR23 pKa = 11.84GRR25 pKa = 11.84RR26 pKa = 11.84VRR28 pKa = 11.84AGRR31 pKa = 11.84GRR33 pKa = 11.84RR34 pKa = 11.84GRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84GGAGPGGRR47 pKa = 11.84AGRR50 pKa = 11.84AARR53 pKa = 11.84GGGRR57 pKa = 11.84DD58 pKa = 3.16RR59 pKa = 11.84RR60 pKa = 3.94

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7670

0

7670

2493908

18

9614

325.2

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.655 ± 0.044

0.749 ± 0.008

6.363 ± 0.025

5.771 ± 0.023

2.769 ± 0.017

9.944 ± 0.026

2.141 ± 0.013

3.039 ± 0.019

1.73 ± 0.019

10.207 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.798 ± 0.011

1.541 ± 0.013

6.394 ± 0.026

2.137 ± 0.014

9.048 ± 0.031

4.278 ± 0.018

5.326 ± 0.023

8.716 ± 0.03

1.474 ± 0.013

1.922 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski