Amino acid dipepetide frequency for Actinomadura sp. WAC 06369

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	26.148AlaAla: 26.148 ± 0.18	1.139AlaCys: 1.139 ± 0.023	9.473AlaAsp: 9.473 ± 0.074	9.674AlaGlu: 9.674 ± 0.075	3.981AlaPhe: 3.981 ± 0.045	14.989AlaGly: 14.989 ± 0.097	2.864AlaHis: 2.864 ± 0.04	3.62AlaIle: 3.62 ± 0.046	2.44AlaLys: 2.44 ± 0.039	14.994AlaLeu: 14.994 ± 0.103	2.669AlaMet: 2.669 ± 0.033	1.813AlaAsn: 1.813 ± 0.03	8.034AlaPro: 8.034 ± 0.071	2.949AlaGln: 2.949 ± 0.035	12.496AlaArg: 12.496 ± 0.097	5.644AlaSer: 5.644 ± 0.045	5.971AlaThr: 5.971 ± 0.057	13.115AlaVal: 13.115 ± 0.107	1.972AlaTrp: 1.972 ± 0.03	2.565AlaTyr: 2.565 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.067CysAla: 1.067 ± 0.023	0.084CysCys: 0.084 ± 0.006	0.507CysAsp: 0.507 ± 0.015	0.398CysGlu: 0.398 ± 0.014	0.199CysPhe: 0.199 ± 0.009	0.972CysGly: 0.972 ± 0.022	0.169CysHis: 0.169 ± 0.009	0.147CysIle: 0.147 ± 0.007	0.108CysLys: 0.108 ± 0.007	0.657CysLeu: 0.657 ± 0.017	0.128CysMet: 0.128 ± 0.008	0.107CysAsn: 0.107 ± 0.007	0.487CysPro: 0.487 ± 0.015	0.126CysGln: 0.126 ± 0.006	0.643CysArg: 0.643 ± 0.018	0.366CysSer: 0.366 ± 0.012	0.456CysThr: 0.456 ± 0.014	0.63CysVal: 0.63 ± 0.017	0.099CysTrp: 0.099 ± 0.007	0.137CysTyr: 0.137 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	9.393AspAla: 9.393 ± 0.074	0.354AspCys: 0.354 ± 0.012	4.288AspAsp: 4.288 ± 0.05	4.262AspGlu: 4.262 ± 0.046	1.529AspPhe: 1.529 ± 0.024	7.515AspGly: 7.515 ± 0.062	1.301AspHis: 1.301 ± 0.027	1.644AspIle: 1.644 ± 0.027	0.804AspLys: 0.804 ± 0.022	6.702AspLeu: 6.702 ± 0.062	0.883AspMet: 0.883 ± 0.019	0.711AspAsn: 0.711 ± 0.016	5.062AspPro: 5.062 ± 0.052	1.145AspGln: 1.145 ± 0.024	5.929AspArg: 5.929 ± 0.053	1.757AspSer: 1.757 ± 0.024	2.551AspThr: 2.551 ± 0.032	5.778AspVal: 5.778 ± 0.057	0.954AspTrp: 0.954 ± 0.023	1.065AspTyr: 1.065 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	7.373GluAla: 7.373 ± 0.06	0.385GluCys: 0.385 ± 0.012	2.981GluAsp: 2.981 ± 0.035	3.384GluGlu: 3.384 ± 0.046	1.589GluPhe: 1.589 ± 0.028	4.11GluGly: 4.11 ± 0.045	1.687GluHis: 1.687 ± 0.028	2.405GluIle: 2.405 ± 0.035	1.206GluLys: 1.206 ± 0.028	6.627GluLeu: 6.627 ± 0.054	0.961GluMet: 0.961 ± 0.021	0.929GluAsn: 0.929 ± 0.018	3.951GluPro: 3.951 ± 0.045	1.834GluGln: 1.834 ± 0.035	6.595GluArg: 6.595 ± 0.066	2.369GluSer: 2.369 ± 0.03	2.865GluThr: 2.865 ± 0.038	4.507GluVal: 4.507 ± 0.046	0.804GluTrp: 0.804 ± 0.02	1.146GluTyr: 1.146 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	4.17PheAla: 4.17 ± 0.05	0.281PheCys: 0.281 ± 0.011	2.075PheAsp: 2.075 ± 0.033	1.521PheGlu: 1.521 ± 0.029	0.859PhePhe: 0.859 ± 0.021	3.304PheGly: 3.304 ± 0.048	0.564PheHis: 0.564 ± 0.015	0.661PheIle: 0.661 ± 0.017	0.425PheLys: 0.425 ± 0.016	2.529PheLeu: 2.529 ± 0.036	0.415PheMet: 0.415 ± 0.013	0.472PheAsn: 0.472 ± 0.014	1.406PhePro: 1.406 ± 0.022	0.597PheGln: 0.597 ± 0.014	2.003PheArg: 2.003 ± 0.035	1.184PheSer: 1.184 ± 0.023	1.817PheThr: 1.817 ± 0.026	2.444PheVal: 2.444 ± 0.034	0.411PheTrp: 0.411 ± 0.012	0.548PheTyr: 0.548 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	12.831GlyAla: 12.831 ± 0.078	0.787GlyCys: 0.787 ± 0.018	6.07GlyAsp: 6.07 ± 0.053	5.496GlyGlu: 5.496 ± 0.052	3.023GlyPhe: 3.023 ± 0.04	10.27GlyGly: 10.27 ± 0.105	2.172GlyHis: 2.172 ± 0.035	3.104GlyIle: 3.104 ± 0.039	2.008GlyLys: 2.008 ± 0.035	9.872GlyLeu: 9.872 ± 0.075	2.206GlyMet: 2.206 ± 0.03	1.469GlyAsn: 1.469 ± 0.028	5.952GlyPro: 5.952 ± 0.063	2.096GlyGln: 2.096 ± 0.035	9.411GlyArg: 9.411 ± 0.069	4.482GlySer: 4.482 ± 0.046	6.146GlyThr: 6.146 ± 0.046	8.126GlyVal: 8.126 ± 0.074	1.681GlyTrp: 1.681 ± 0.026	2.237GlyTyr: 2.237 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.756HisAla: 2.756 ± 0.036	0.193HisCys: 0.193 ± 0.01	1.289HisAsp: 1.289 ± 0.025	1.13HisGlu: 1.13 ± 0.021	0.556HisPhe: 0.556 ± 0.016	2.524HisGly: 2.524 ± 0.037	0.592HisHis: 0.592 ± 0.018	0.578HisIle: 0.578 ± 0.016	0.26HisLys: 0.26 ± 0.01	2.24HisLeu: 2.24 ± 0.034	0.34HisMet: 0.34 ± 0.014	0.29HisAsn: 0.29 ± 0.011	1.722HisPro: 1.722 ± 0.029	0.445HisGln: 0.445 ± 0.014	2.183HisArg: 2.183 ± 0.036	0.773HisSer: 0.773 ± 0.019	0.945HisThr: 0.945 ± 0.018	1.818HisVal: 1.818 ± 0.028	0.334HisTrp: 0.334 ± 0.011	0.447HisTyr: 0.447 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.929IleAla: 4.929 ± 0.047	0.248IleCys: 0.248 ± 0.01	2.256IleAsp: 2.256 ± 0.033	2.092IleGlu: 2.092 ± 0.03	0.669IlePhe: 0.669 ± 0.021	3.557IleGly: 3.557 ± 0.04	0.47IleHis: 0.47 ± 0.016	0.878IleIle: 0.878 ± 0.02	0.586IleLys: 0.586 ± 0.018	2.249IleLeu: 2.249 ± 0.033	0.496IleMet: 0.496 ± 0.014	0.589IleAsn: 0.589 ± 0.017	1.549IlePro: 1.549 ± 0.025	0.541IleGln: 0.541 ± 0.016	2.121IleArg: 2.121 ± 0.03	1.326IleSer: 1.326 ± 0.029	1.954IleThr: 1.954 ± 0.033	3.068IleVal: 3.068 ± 0.038	0.348IleTrp: 0.348 ± 0.013	0.464IleTyr: 0.464 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.359LysAla: 2.359 ± 0.04	0.09LysCys: 0.09 ± 0.006	1.001LysAsp: 1.001 ± 0.023	0.982LysGlu: 0.982 ± 0.027	0.381LysPhe: 0.381 ± 0.013	1.469LysGly: 1.469 ± 0.03	0.377LysHis: 0.377 ± 0.013	0.806LysIle: 0.806 ± 0.019	0.558LysLys: 0.558 ± 0.02	1.592LysLeu: 1.592 ± 0.031	0.355LysMet: 0.355 ± 0.014	0.372LysAsn: 0.372 ± 0.012	1.118LysPro: 1.118 ± 0.025	0.487LysGln: 0.487 ± 0.015	1.345LysArg: 1.345 ± 0.026	0.857LysSer: 0.857 ± 0.022	1.012LysThr: 1.012 ± 0.021	1.565LysVal: 1.565 ± 0.03	0.219LysTrp: 0.219 ± 0.011	0.355LysTyr: 0.355 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	16.556LeuAla: 16.556 ± 0.101	0.755LeuCys: 0.755 ± 0.018	7.216LeuAsp: 7.216 ± 0.063	4.929LeuGlu: 4.929 ± 0.05	2.608LeuPhe: 2.608 ± 0.037	9.5LeuGly: 9.5 ± 0.075	2.136LeuHis: 2.136 ± 0.034	3.01LeuIle: 3.01 ± 0.045	1.57LeuLys: 1.57 ± 0.03	10.899LeuLeu: 10.899 ± 0.104	1.545LeuMet: 1.545 ± 0.028	1.544LeuAsn: 1.544 ± 0.028	6.379LeuPro: 6.379 ± 0.054	1.884LeuGln: 1.884 ± 0.027	9.285LeuArg: 9.285 ± 0.067	4.41LeuSer: 4.41 ± 0.049	5.946LeuThr: 5.946 ± 0.051	8.966LeuVal: 8.966 ± 0.069	1.245LeuTrp: 1.245 ± 0.025	1.683LeuTyr: 1.683 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.376MetAla: 2.376 ± 0.032	0.131MetCys: 0.131 ± 0.008	0.971MetAsp: 0.971 ± 0.019	0.79MetGlu: 0.79 ± 0.022	0.53MetPhe: 0.53 ± 0.016	1.348MetGly: 1.348 ± 0.025	0.34MetHis: 0.34 ± 0.012	0.733MetIle: 0.733 ± 0.021	0.377MetLys: 0.377 ± 0.011	1.872MetLeu: 1.872 ± 0.03	0.306MetMet: 0.306 ± 0.009	0.409MetAsn: 0.409 ± 0.013	1.151MetPro: 1.151 ± 0.025	0.421MetGln: 0.421 ± 0.013	1.652MetArg: 1.652 ± 0.025	1.216MetSer: 1.216 ± 0.022	1.479MetThr: 1.479 ± 0.027	1.34MetVal: 1.34 ± 0.024	0.219MetTrp: 0.219 ± 0.011	0.32MetTyr: 0.32 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.041AsnAla: 2.041 ± 0.026	0.152AsnCys: 0.152 ± 0.008	0.811AsnAsp: 0.811 ± 0.019	0.766AsnGlu: 0.766 ± 0.018	0.41AsnPhe: 0.41 ± 0.014	1.734AsnGly: 1.734 ± 0.03	0.326AsnHis: 0.326 ± 0.011	0.558AsnIle: 0.558 ± 0.016	0.28AsnLys: 0.28 ± 0.011	1.571AsnLeu: 1.571 ± 0.029	0.276AsnMet: 0.276 ± 0.012	0.297AsnAsn: 0.297 ± 0.013	1.197AsnPro: 1.197 ± 0.026	0.368AsnGln: 0.368 ± 0.014	1.231AsnArg: 1.231 ± 0.022	0.601AsnSer: 0.601 ± 0.019	0.859AsnThr: 0.859 ± 0.021	1.367AsnVal: 1.367 ± 0.027	0.221AsnTrp: 0.221 ± 0.01	0.341AsnTyr: 0.341 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.462ProAla: 9.462 ± 0.087	0.329ProCys: 0.329 ± 0.013	5.479ProAsp: 5.479 ± 0.06	4.369ProGlu: 4.369 ± 0.044	1.651ProPhe: 1.651 ± 0.028	7.606ProGly: 7.606 ± 0.074	1.364ProHis: 1.364 ± 0.025	1.42ProIle: 1.42 ± 0.027	1.065ProLys: 1.065 ± 0.027	5.297ProLeu: 5.297 ± 0.054	1.071ProMet: 1.071 ± 0.02	0.919ProAsn: 0.919 ± 0.023	4.521ProPro: 4.521 ± 0.07	1.316ProGln: 1.316 ± 0.029	4.585ProArg: 4.585 ± 0.044	3.082ProSer: 3.082 ± 0.039	2.549ProThr: 2.549 ± 0.036	5.631ProVal: 5.631 ± 0.05	0.97ProTrp: 0.97 ± 0.018	1.252ProTyr: 1.252 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	2.96GlnAla: 2.96 ± 0.041	0.15GlnCys: 0.15 ± 0.008	1.112GlnAsp: 1.112 ± 0.022	1.187GlnGlu: 1.187 ± 0.029	0.587GlnPhe: 0.587 ± 0.015	1.855GlnGly: 1.855 ± 0.032	0.456GlnHis: 0.456 ± 0.014	0.974GlnIle: 0.974 ± 0.023	0.465GlnLys: 0.465 ± 0.016	1.977GlnLeu: 1.977 ± 0.029	0.425GlnMet: 0.425 ± 0.014	0.438GlnAsn: 0.438 ± 0.013	1.203GlnPro: 1.203 ± 0.029	0.804GlnGln: 0.804 ± 0.025	2.02GlnArg: 2.02 ± 0.032	0.888GlnSer: 0.888 ± 0.02	1.043GlnThr: 1.043 ± 0.02	2.036GlnVal: 2.036 ± 0.03	0.336GlnTrp: 0.336 ± 0.012	0.45GlnTyr: 0.45 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	12.15ArgAla: 12.15 ± 0.097	0.619ArgCys: 0.619 ± 0.018	5.626ArgAsp: 5.626 ± 0.049	5.245ArgGlu: 5.245 ± 0.052	2.613ArgPhe: 2.613 ± 0.034	6.876ArgGly: 6.876 ± 0.051	2.192ArgHis: 2.192 ± 0.029	3.213ArgIle: 3.213 ± 0.037	1.472ArgLys: 1.472 ± 0.026	10.025ArgLeu: 10.025 ± 0.085	1.954ArgMet: 1.954 ± 0.028	1.338ArgAsn: 1.338 ± 0.025	6.098ArgPro: 6.098 ± 0.066	1.818ArgGln: 1.818 ± 0.027	9.744ArgArg: 9.744 ± 0.092	3.707ArgSer: 3.707 ± 0.045	5.415ArgThr: 5.415 ± 0.045	6.991ArgVal: 6.991 ± 0.065	1.486ArgTrp: 1.486 ± 0.027	1.895ArgTyr: 1.895 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	5.744SerAla: 5.744 ± 0.046	0.35SerCys: 0.35 ± 0.012	2.258SerAsp: 2.258 ± 0.03	1.969SerGlu: 1.969 ± 0.031	1.369SerPhe: 1.369 ± 0.026	5.53SerGly: 5.53 ± 0.052	0.784SerHis: 0.784 ± 0.021	1.324SerIle: 1.324 ± 0.026	0.786SerLys: 0.786 ± 0.021	4.116SerLeu: 4.116 ± 0.038	1.003SerMet: 1.003 ± 0.019	0.674SerAsn: 0.674 ± 0.017	2.888SerPro: 2.888 ± 0.035	0.872SerGln: 0.872 ± 0.019	3.447SerArg: 3.447 ± 0.037	2.126SerSer: 2.126 ± 0.035	2.213SerThr: 2.213 ± 0.032	3.616SerVal: 3.616 ± 0.045	0.784SerTrp: 0.784 ± 0.02	0.929SerTyr: 0.929 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	8.024ThrAla: 8.024 ± 0.066	0.384ThrCys: 0.384 ± 0.013	3.063ThrAsp: 3.063 ± 0.038	2.677ThrGlu: 2.677 ± 0.031	1.505ThrPhe: 1.505 ± 0.025	6.425ThrGly: 6.425 ± 0.051	0.925ThrHis: 0.925 ± 0.023	1.672ThrIle: 1.672 ± 0.028	0.826ThrLys: 0.826 ± 0.02	5.069ThrLeu: 5.069 ± 0.051	0.957ThrMet: 0.957 ± 0.022	0.826ThrAsn: 0.826 ± 0.018	3.414ThrPro: 3.414 ± 0.041	0.915ThrGln: 0.915 ± 0.022	3.827ThrArg: 3.827 ± 0.04	2.506ThrSer: 2.506 ± 0.034	2.796ThrThr: 2.796 ± 0.041	5.53ThrVal: 5.53 ± 0.05	0.848ThrTrp: 0.848 ± 0.02	1.077ThrTyr: 1.077 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	11.89ValAla: 11.89 ± 0.088	0.719ValCys: 0.719 ± 0.017	5.021ValAsp: 5.021 ± 0.046	4.934ValGlu: 4.934 ± 0.044	2.543ValPhe: 2.543 ± 0.036	6.653ValGly: 6.653 ± 0.064	1.989ValHis: 1.989 ± 0.03	2.673ValIle: 2.673 ± 0.036	1.465ValLys: 1.465 ± 0.033	10.051ValLeu: 10.051 ± 0.082	1.439ValMet: 1.439 ± 0.025	1.529ValAsn: 1.529 ± 0.028	5.968ValPro: 5.968 ± 0.061	1.925ValGln: 1.925 ± 0.027	8.518ValArg: 8.518 ± 0.073	3.919ValSer: 3.919 ± 0.046	5.1ValThr: 5.1 ± 0.043	8.109ValVal: 8.109 ± 0.081	1.138ValTrp: 1.138 ± 0.02	1.571ValTyr: 1.571 ± 0.023	0.0ValXaa: 0.0 ± 0.0
Trp	1.711TrpAla: 1.711 ± 0.029	0.156TrpCys: 0.156 ± 0.008	0.882TrpAsp: 0.882 ± 0.023	0.747TrpGlu: 0.747 ± 0.017	0.503TrpPhe: 0.503 ± 0.016	1.066TrpGly: 1.066 ± 0.02	0.33TrpHis: 0.33 ± 0.011	0.491TrpIle: 0.491 ± 0.015	0.29TrpLys: 0.29 ± 0.011	1.7TrpLeu: 1.7 ± 0.029	0.266TrpMet: 0.266 ± 0.011	0.351TrpAsn: 0.351 ± 0.012	0.831TrpPro: 0.831 ± 0.02	0.4TrpGln: 0.4 ± 0.014	1.533TrpArg: 1.533 ± 0.025	0.808TrpSer: 0.808 ± 0.018	1.102TrpThr: 1.102 ± 0.021	0.913TrpVal: 0.913 ± 0.019	0.328TrpTrp: 0.328 ± 0.012	0.331TrpTyr: 0.331 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.612TyrAla: 2.612 ± 0.031	0.178TyrCys: 0.178 ± 0.009	1.249TyrAsp: 1.249 ± 0.027	1.156TyrGlu: 1.156 ± 0.024	0.582TyrPhe: 0.582 ± 0.017	2.136TyrGly: 2.136 ± 0.03	0.378TyrHis: 0.378 ± 0.013	0.48TyrIle: 0.48 ± 0.014	0.307TyrLys: 0.307 ± 0.013	2.028TyrLeu: 2.028 ± 0.03	0.285TyrMet: 0.285 ± 0.011	0.329TyrAsn: 0.329 ± 0.012	1.008TyrPro: 1.008 ± 0.022	0.422TyrGln: 0.422 ± 0.015	1.909TyrArg: 1.909 ± 0.026	0.762TyrSer: 0.762 ± 0.017	1.045TyrThr: 1.045 ± 0.024	1.604TyrVal: 1.604 ± 0.026	0.342TyrTrp: 0.342 ± 0.012	0.409TyrTyr: 0.409 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7670 proteins (2493908 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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