Martelella endophytica
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4020 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5LKK0|A0A0D5LKK0_9RHIZ Transcriptional regulator OS=Martelella endophytica OX=1486262 GN=TM49_00245 PE=4 SV=1
MM1 pKa = 8.23 DD2 pKa = 4.12 SRR4 pKa = 11.84 KK5 pKa = 9.92 FSDD8 pKa = 3.36 SNAVFFKK15 pKa = 10.79 RR16 pKa = 11.84 KK17 pKa = 9.57 GSLITQTALSGVFAIGAVMLASSSSFSADD46 pKa = 3.3 RR47 pKa = 11.84 SWDD50 pKa = 3.61 LFPTNGNFVVGSNWSGNTAPGAADD74 pKa = 3.47 NANIGASFNSPWLDD88 pKa = 3.03 QTYY91 pKa = 10.09 SVQSVSVSGGGSFTIRR107 pKa = 11.84 NGGNLTADD115 pKa = 3.31 TMTVGGVLSSITIASGGAFNGQVSATGGTFTNSGTVNGAASISGGIATNSGSVTGMVTNSSIGLVLVPGFTNASGGSIGGLTNSGIAVNEE205 pKa = 3.96 NGASIGTLSNTNGTFSNSGTVTGTTSISGGVVTNNSDD242 pKa = 3.71 GVFEE246 pKa = 5.15 GATSMTGGITTNNGTINGATTVSGALTSFTNNGTINNTLGITSGIVTNNGSVTGMVTNSSIGIIVAPGFTNASGGSIGGLANSGIAVNEE335 pKa = 3.96 NGASIGTLSNTNGTFSNSGTVTGTTSISGGVVTNNSDD372 pKa = 3.71 GVFEE376 pKa = 5.15 GATSMTGGITTNNGTINGATTVSGALTSFTNNGTINNTLGITSGIVTNNGSVTGMVTNSSIGIVVAPGFTNAGGGSIGGLSNSGIAVNEE465 pKa = 3.96 NGASIGTLSNTNGTFSNSGTVTGTTSISGGVVTNNSDD502 pKa = 3.71 GVFEE506 pKa = 5.15 GATSMTGGILTNNGSIDD523 pKa = 3.58 NTLGITGGVATNNGTVAGMVTNSSFGLVLVPGFTNASGGSIGGLTNSGIAVNEE576 pKa = 3.92 NGGTIGTLSNTGGVFTNSGTVTGTTTVSGGVVTNTSDD613 pKa = 3.8 GVFEE617 pKa = 5.16 GATSMTGGITTNNGTINGATTVSGVATTFTNTGTIDD653 pKa = 3.33 NTLGVTGGIVTNTGTVTGATGVSGGLLTNTGTIDD687 pKa = 3.39 NTLGVAGGVVINTGTVTGATGVSAGLLTNTGTIDD721 pKa = 3.36 NTLGITGGVVVNAGTVTGAAGISGGLLTNTGTLEE755 pKa = 3.98 STLDD759 pKa = 3.47 VLGGFVANEE768 pKa = 3.77 AGGDD772 pKa = 3.89 VVGLTTIYY780 pKa = 11.04 DD781 pKa = 3.69 GDD783 pKa = 3.9 VTNGLISAANFGTVINYY800 pKa = 7.49 STGDD804 pKa = 3.22 INSNGFTNGSFGRR817 pKa = 11.84 TEE819 pKa = 3.68 ALVNHH824 pKa = 6.87 GSSVNLIGGSIGTATNNAGTFTNSGSIDD852 pKa = 3.31 GALVVNGGTVDD863 pKa = 3.4 NNGNGVLNNGTVGGLTTISNDD884 pKa = 3.22 GLVTNSGTLADD895 pKa = 4.26 VVNGSYY901 pKa = 10.73 AGGSGSGFVNQAGGVAGALTNSGGATNEE929 pKa = 3.96 AGGTLASVLNTAGVFTNAGTVTGTLDD955 pKa = 3.47 VTGGSADD962 pKa = 3.52 NTGSVAGLTTISNDD976 pKa = 3.14 GVATNSGTLADD987 pKa = 3.9 VVNGSFAGGSGFGFVNQSGGVAGALTNSGGATNMAGATLASVTNDD1032 pKa = 2.51 AGYY1035 pKa = 7.77 FTNAGTVSGSVAVNAGTAEE1054 pKa = 4.01 NTGLIGGAVDD1064 pKa = 3.2 IADD1067 pKa = 4.22 NGTMTNSGQIDD1078 pKa = 4.38 GLTTNAGLFATTGTLNGGLVNSGIASIRR1106 pKa = 11.84 GTINGAIEE1114 pKa = 4.11 NSSLLDD1120 pKa = 3.47 DD1121 pKa = 4.32 TFSLTGDD1128 pKa = 3.62 LVGNGTLSNQGTFTAWGGYY1147 pKa = 7.06 TISGIDD1153 pKa = 3.36 IDD1155 pKa = 5.34 NSGTLNASNYY1165 pKa = 8.29 KK1166 pKa = 9.52 TDD1168 pKa = 3.21 TGALKK1173 pKa = 9.33 TAGTSATTLTVDD1185 pKa = 3.67 SDD1187 pKa = 3.38 ITRR1190 pKa = 11.84 GGRR1193 pKa = 11.84 IALGTYY1199 pKa = 10.75 ADD1201 pKa = 3.88 TTTDD1205 pKa = 3.57 QIQVTGDD1212 pKa = 3.48 FSGGDD1217 pKa = 3.65 YY1218 pKa = 11.35 DD1219 pKa = 4.59 LALYY1223 pKa = 9.24 GLNYY1227 pKa = 10.22 LDD1229 pKa = 3.91 TPEE1232 pKa = 4.25 EE1233 pKa = 4.08 RR1234 pKa = 11.84 TLITIDD1240 pKa = 3.38 GTYY1243 pKa = 9.57 SASVGDD1249 pKa = 4.31 VYY1251 pKa = 11.67 GLGNTANPLIVNSVRR1266 pKa = 11.84 EE1267 pKa = 4.16 TANSVILTTALDD1279 pKa = 3.6 MTPISGTLGGIYY1291 pKa = 8.91 ATQAFTGITTNRR1303 pKa = 11.84 FDD1305 pKa = 4.06 DD1306 pKa = 4.28 SYY1308 pKa = 10.73 VTAVDD1313 pKa = 4.16 GAGKK1317 pKa = 10.11 GCSPGTYY1324 pKa = 8.74 TRR1326 pKa = 11.84 MNGGSLNTASTASSAGSVSAKK1347 pKa = 8.98 TDD1349 pKa = 3.04 SDD1351 pKa = 3.42 ISFGGVEE1358 pKa = 4.25 LGLDD1362 pKa = 3.69 YY1363 pKa = 11.71 GCFDD1367 pKa = 4.15 LGKK1370 pKa = 10.65 AGGQVNFGLLGGLNSGSVSSNEE1392 pKa = 3.77 TVAGIGYY1399 pKa = 9.46 SGQSTFDD1406 pKa = 3.04 QQYY1409 pKa = 7.79 FGGYY1413 pKa = 9.92 ASYY1416 pKa = 10.9 LAGPFVAQLRR1426 pKa = 11.84 TTYY1429 pKa = 10.79 GRR1431 pKa = 11.84 TAYY1434 pKa = 10.39 DD1435 pKa = 3.34 IDD1437 pKa = 3.68 QMRR1440 pKa = 11.84 TASGLSTSVFDD1451 pKa = 5.28 QDD1453 pKa = 5.19 GFDD1456 pKa = 3.83 ANQYY1460 pKa = 6.84 TASGYY1465 pKa = 9.92 LAYY1468 pKa = 9.99 GFNMDD1473 pKa = 4.94 RR1474 pKa = 11.84 FTLLPSIGLSYY1485 pKa = 11.08 SNSVFNDD1492 pKa = 3.43 DD1493 pKa = 4.11 LQVASGSPAQISFDD1507 pKa = 4.13 DD1508 pKa = 3.91 MEE1510 pKa = 4.68 TLVGSIGLAASASFTLDD1527 pKa = 3.46 DD1528 pKa = 4.18 NRR1530 pKa = 11.84 GTIQPFLAGTYY1541 pKa = 9.82 YY1542 pKa = 11.34 NNFADD1547 pKa = 5.72 DD1548 pKa = 3.41 PTAYY1552 pKa = 9.51 YY1553 pKa = 10.45 VSSAGIRR1560 pKa = 11.84 TPINTSTVDD1569 pKa = 3.17 NYY1571 pKa = 11.75 GEE1573 pKa = 3.9 ISVGFDD1579 pKa = 3.2 YY1580 pKa = 9.06 LTPPTSVLRR1589 pKa = 11.84 QVSGQVRR1596 pKa = 11.84 ADD1598 pKa = 3.7 FPVGKK1603 pKa = 9.41 DD1604 pKa = 3.23 VSGAQVSLSMRR1615 pKa = 11.84 AQFF1618 pKa = 4.51
Molecular weight: 157.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.579
IPC_protein 3.643
Toseland 3.401
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.63
Rodwell 3.465
Grimsley 3.3
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.075
Thurlkill 3.465
EMBOSS 3.63
Sillero 3.77
Patrickios 0.896
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A0D5LPK4|A0A0D5LPK4_9RHIZ HTH marR-type domain-containing protein OS=Martelella endophytica OX=1486262 GN=TM49_11500 PE=4 SV=1
MM1 pKa = 7.58 PRR3 pKa = 11.84 QGPGNPGFSRR13 pKa = 11.84 LTADD17 pKa = 3.72 RR18 pKa = 11.84 ASRR21 pKa = 11.84 AASGARR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGGRR34 pKa = 11.84 HH35 pKa = 3.97 SRR37 pKa = 11.84 ARR39 pKa = 11.84 DD40 pKa = 3.18 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 SPWRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 LPASARR56 pKa = 11.84 SANRR60 pKa = 11.84 ARR62 pKa = 11.84 APRR65 pKa = 3.86
Molecular weight: 7.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.276
Rodwell 12.296
Grimsley 12.837
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.018
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4020
0
4020
1282831
29
2827
319.1
34.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.013 ± 0.05
0.78 ± 0.01
5.939 ± 0.033
6.047 ± 0.038
4.058 ± 0.027
8.611 ± 0.055
1.967 ± 0.017
5.64 ± 0.027
3.388 ± 0.031
9.887 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.019
2.832 ± 0.025
4.943 ± 0.027
2.767 ± 0.022
6.447 ± 0.042
5.491 ± 0.032
5.443 ± 0.031
7.301 ± 0.034
1.293 ± 0.017
2.442 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here