Staphylococcus phage SpT152

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coventryvirus; Staphylococcus virus SpT152

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MF70|A0A1J0MF70_9CAUD CRISPR associated protein OS=Staphylococcus phage SpT152 OX=1913446 GN=SpT152_008 PE=4 SV=1
MM1 pKa = 7.48LKK3 pKa = 8.95STAQQALFDD12 pKa = 4.53YY13 pKa = 10.26IYY15 pKa = 10.92SALEE19 pKa = 4.19GYY21 pKa = 10.36GFDD24 pKa = 4.27VIDD27 pKa = 4.18FKK29 pKa = 11.47EE30 pKa = 4.32LNTQISYY37 pKa = 10.27PFFVVRR43 pKa = 11.84NVDD46 pKa = 3.35INKK49 pKa = 8.74TKK51 pKa = 11.19YY52 pKa = 11.07NMDD55 pKa = 3.45NFGGEE60 pKa = 3.97LTATIDD66 pKa = 3.13FWTYY70 pKa = 9.65ADD72 pKa = 3.57DD73 pKa = 4.5RR74 pKa = 11.84GRR76 pKa = 11.84HH77 pKa = 5.41DD78 pKa = 4.51SIVYY82 pKa = 10.41SVDD85 pKa = 3.4TEE87 pKa = 4.0FSNIDD92 pKa = 3.56SVEE95 pKa = 4.31GYY97 pKa = 10.74QLMIDD102 pKa = 4.12DD103 pKa = 5.04MDD105 pKa = 3.72IKK107 pKa = 10.11TLNDD111 pKa = 3.26VEE113 pKa = 4.51NSDD116 pKa = 3.91RR117 pKa = 11.84QLLHH121 pKa = 5.95TVFIANYY128 pKa = 10.15KK129 pKa = 10.32LFF131 pKa = 4.2

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MF46|A0A1J0MF46_9CAUD Uncharacterized protein OS=Staphylococcus phage SpT152 OX=1913446 GN=SpT152_004 PE=4 SV=1
MM1 pKa = 7.44ILSNSVRR8 pKa = 11.84QRR10 pKa = 11.84YY11 pKa = 7.38RR12 pKa = 11.84TDD14 pKa = 3.24TVGKK18 pKa = 8.39TPTEE22 pKa = 3.9LQKK25 pKa = 10.55EE26 pKa = 4.12LRR28 pKa = 11.84KK29 pKa = 10.13RR30 pKa = 11.84GVRR33 pKa = 11.84GFVVNVSHH41 pKa = 6.88NRR43 pKa = 11.84VTMLVDD49 pKa = 3.23RR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.25VKK54 pKa = 9.98TNKK57 pKa = 9.82EE58 pKa = 3.97CMRR61 pKa = 11.84CLTSTEE67 pKa = 3.88TRR69 pKa = 11.84KK70 pKa = 10.33

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12519

47

1039

184.1

21.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.943 ± 0.454

0.423 ± 0.084

6.75 ± 0.385

7.461 ± 0.467

4.266 ± 0.231

4.984 ± 0.283

1.606 ± 0.168

7.453 ± 0.372

9.514 ± 0.367

8.068 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.148

6.662 ± 0.24

2.524 ± 0.183

3.778 ± 0.181

3.914 ± 0.244

6.247 ± 0.273

6.015 ± 0.293

6.414 ± 0.248

1.03 ± 0.125

4.465 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski