Amino acid dipepetide frequency for Staphylococcus phage SpT152

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.476AlaAla: 2.476 ± 0.442
0.399AlaCys: 0.399 ± 0.171
3.914AlaAsp: 3.914 ± 0.495
4.793AlaGlu: 4.793 ± 0.682
2.556AlaPhe: 2.556 ± 0.435
2.476AlaGly: 2.476 ± 0.604
0.799AlaHis: 0.799 ± 0.225
6.151AlaIle: 6.151 ± 1.076
5.911AlaLys: 5.911 ± 0.809
5.113AlaLeu: 5.113 ± 0.669
0.959AlaMet: 0.959 ± 0.232
4.713AlaAsn: 4.713 ± 0.562
1.198AlaPro: 1.198 ± 0.274
2.796AlaGln: 2.796 ± 0.455
2.317AlaArg: 2.317 ± 0.46
2.876AlaSer: 2.876 ± 0.523
4.234AlaThr: 4.234 ± 0.563
2.956AlaVal: 2.956 ± 0.436
0.559AlaTrp: 0.559 ± 0.18
2.237AlaTyr: 2.237 ± 0.457
0.0AlaXaa: 0.0 ± 0.0
Cys
0.08CysAla: 0.08 ± 0.088
0.0CysCys: 0.0 ± 0.0
0.32CysAsp: 0.32 ± 0.159
0.08CysGlu: 0.08 ± 0.089
0.16CysPhe: 0.16 ± 0.117
0.24CysGly: 0.24 ± 0.136
0.24CysHis: 0.24 ± 0.121
0.399CysIle: 0.399 ± 0.184
0.639CysLys: 0.639 ± 0.214
0.32CysLeu: 0.32 ± 0.17
0.16CysMet: 0.16 ± 0.099
0.32CysAsn: 0.32 ± 0.176
0.0CysPro: 0.0 ± 0.0
0.08CysGln: 0.08 ± 0.083
0.399CysArg: 0.399 ± 0.203
0.24CysSer: 0.24 ± 0.142
0.24CysThr: 0.24 ± 0.128
0.08CysVal: 0.08 ± 0.07
0.08CysTrp: 0.08 ± 0.081
0.16CysTyr: 0.16 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
3.515AspAla: 3.515 ± 0.477
0.08AspCys: 0.08 ± 0.083
6.71AspAsp: 6.71 ± 0.882
6.87AspGlu: 6.87 ± 1.039
3.355AspPhe: 3.355 ± 0.567
4.154AspGly: 4.154 ± 0.489
0.879AspHis: 0.879 ± 0.238
4.713AspIle: 4.713 ± 0.644
6.151AspLys: 6.151 ± 0.75
5.113AspLeu: 5.113 ± 0.811
1.598AspMet: 1.598 ± 0.332
4.074AspAsn: 4.074 ± 0.589
0.959AspPro: 0.959 ± 0.225
1.039AspGln: 1.039 ± 0.296
1.917AspArg: 1.917 ± 0.308
5.033AspSer: 5.033 ± 0.67
2.476AspThr: 2.476 ± 0.453
4.873AspVal: 4.873 ± 0.528
0.32AspTrp: 0.32 ± 0.139
3.675AspTyr: 3.675 ± 0.593
0.0AspXaa: 0.0 ± 0.0
Glu
5.512GluAla: 5.512 ± 0.548
0.559GluCys: 0.559 ± 0.209
3.355GluAsp: 3.355 ± 0.523
6.231GluGlu: 6.231 ± 0.982
3.914GluPhe: 3.914 ± 0.49
3.036GluGly: 3.036 ± 0.43
0.639GluHis: 0.639 ± 0.211
5.512GluIle: 5.512 ± 0.796
4.474GluLys: 4.474 ± 0.644
6.79GluLeu: 6.79 ± 0.776
3.116GluMet: 3.116 ± 0.486
4.553GluAsn: 4.553 ± 0.642
1.678GluPro: 1.678 ± 0.409
3.515GluGln: 3.515 ± 0.507
4.234GluArg: 4.234 ± 0.666
4.394GluSer: 4.394 ± 0.812
3.275GluThr: 3.275 ± 0.563
5.592GluVal: 5.592 ± 0.676
0.799GluTrp: 0.799 ± 0.249
3.435GluTyr: 3.435 ± 0.505
0.0GluXaa: 0.0 ± 0.0
Phe
2.237PheAla: 2.237 ± 0.492
0.16PheCys: 0.16 ± 0.107
2.237PheAsp: 2.237 ± 0.433
3.675PheGlu: 3.675 ± 0.588
1.837PhePhe: 1.837 ± 0.388
3.195PheGly: 3.195 ± 0.552
0.24PheHis: 0.24 ± 0.133
3.275PheIle: 3.275 ± 0.505
4.873PheLys: 4.873 ± 0.391
2.956PheLeu: 2.956 ± 0.465
1.198PheMet: 1.198 ± 0.327
3.515PheAsn: 3.515 ± 0.519
0.879PhePro: 0.879 ± 0.226
0.879PheGln: 0.879 ± 0.326
1.598PheArg: 1.598 ± 0.38
1.917PheSer: 1.917 ± 0.442
2.397PheThr: 2.397 ± 0.426
3.435PheVal: 3.435 ± 0.617
0.559PheTrp: 0.559 ± 0.21
1.518PheTyr: 1.518 ± 0.356
0.0PheXaa: 0.0 ± 0.0
Gly
2.956GlyAla: 2.956 ± 0.697
0.24GlyCys: 0.24 ± 0.135
2.556GlyAsp: 2.556 ± 0.379
3.755GlyGlu: 3.755 ± 0.698
2.237GlyPhe: 2.237 ± 0.472
2.317GlyGly: 2.317 ± 0.387
0.959GlyHis: 0.959 ± 0.244
3.755GlyIle: 3.755 ± 0.454
4.553GlyLys: 4.553 ± 0.564
4.713GlyLeu: 4.713 ± 0.675
0.959GlyMet: 0.959 ± 0.232
2.157GlyAsn: 2.157 ± 0.4
0.24GlyPro: 0.24 ± 0.141
1.757GlyGln: 1.757 ± 0.39
2.237GlyArg: 2.237 ± 0.375
2.476GlySer: 2.476 ± 0.395
3.914GlyThr: 3.914 ± 0.479
4.633GlyVal: 4.633 ± 0.679
0.32GlyTrp: 0.32 ± 0.17
3.116GlyTyr: 3.116 ± 0.48
0.0GlyXaa: 0.0 ± 0.0
His
0.719HisAla: 0.719 ± 0.251
0.24HisCys: 0.24 ± 0.123
1.118HisAsp: 1.118 ± 0.274
0.639HisGlu: 0.639 ± 0.236
0.719HisPhe: 0.719 ± 0.291
1.118HisGly: 1.118 ± 0.356
0.24HisHis: 0.24 ± 0.122
1.598HisIle: 1.598 ± 0.403
1.198HisLys: 1.198 ± 0.317
1.598HisLeu: 1.598 ± 0.347
0.32HisMet: 0.32 ± 0.142
0.879HisAsn: 0.879 ± 0.257
0.479HisPro: 0.479 ± 0.201
1.118HisGln: 1.118 ± 0.285
0.32HisArg: 0.32 ± 0.179
0.799HisSer: 0.799 ± 0.245
1.039HisThr: 1.039 ± 0.298
0.799HisVal: 0.799 ± 0.253
0.08HisTrp: 0.08 ± 0.076
1.039HisTyr: 1.039 ± 0.28
0.0HisXaa: 0.0 ± 0.0
Ile
4.394IleAla: 4.394 ± 0.68
0.399IleCys: 0.399 ± 0.164
6.87IleAsp: 6.87 ± 0.656
5.193IleGlu: 5.193 ± 0.806
3.355IlePhe: 3.355 ± 0.625
3.435IleGly: 3.435 ± 0.677
1.039IleHis: 1.039 ± 0.27
4.154IleIle: 4.154 ± 0.572
8.148IleLys: 8.148 ± 0.819
3.994IleLeu: 3.994 ± 0.512
0.879IleMet: 0.879 ± 0.316
5.832IleAsn: 5.832 ± 0.731
2.876IlePro: 2.876 ± 0.394
2.476IleGln: 2.476 ± 0.477
2.556IleArg: 2.556 ± 0.439
5.272IleSer: 5.272 ± 0.601
4.474IleThr: 4.474 ± 0.568
4.713IleVal: 4.713 ± 0.463
0.799IleTrp: 0.799 ± 0.26
3.675IleTyr: 3.675 ± 0.583
0.0IleXaa: 0.0 ± 0.0
Lys
6.231LysAla: 6.231 ± 0.732
0.08LysCys: 0.08 ± 0.08
6.87LysAsp: 6.87 ± 0.838
7.11LysGlu: 7.11 ± 1.04
3.994LysPhe: 3.994 ± 0.524
4.873LysGly: 4.873 ± 0.637
1.438LysHis: 1.438 ± 0.35
6.95LysIle: 6.95 ± 0.732
7.988LysLys: 7.988 ± 1.035
7.749LysLeu: 7.749 ± 0.77
2.876LysMet: 2.876 ± 0.493
5.193LysAsn: 5.193 ± 0.606
3.275LysPro: 3.275 ± 1.023
5.193LysGln: 5.193 ± 0.583
3.834LysArg: 3.834 ± 0.639
4.953LysSer: 4.953 ± 0.646
5.832LysThr: 5.832 ± 0.617
6.151LysVal: 6.151 ± 0.672
0.799LysTrp: 0.799 ± 0.228
3.755LysTyr: 3.755 ± 0.673
0.0LysXaa: 0.0 ± 0.0
Leu
4.953LeuAla: 4.953 ± 0.526
0.24LeuCys: 0.24 ± 0.137
5.991LeuAsp: 5.991 ± 0.713
6.151LeuGlu: 6.151 ± 0.674
3.116LeuPhe: 3.116 ± 0.423
3.195LeuGly: 3.195 ± 0.591
1.278LeuHis: 1.278 ± 0.331
5.752LeuIle: 5.752 ± 0.736
7.509LeuLys: 7.509 ± 0.806
6.63LeuLeu: 6.63 ± 0.742
2.796LeuMet: 2.796 ± 0.477
6.151LeuAsn: 6.151 ± 0.722
1.997LeuPro: 1.997 ± 0.452
3.195LeuGln: 3.195 ± 0.399
3.195LeuArg: 3.195 ± 0.564
5.592LeuSer: 5.592 ± 0.746
5.272LeuThr: 5.272 ± 0.687
4.234LeuVal: 4.234 ± 0.646
1.039LeuTrp: 1.039 ± 0.363
2.397LeuTyr: 2.397 ± 0.403
0.0LeuXaa: 0.0 ± 0.0
Met
1.438MetAla: 1.438 ± 0.313
0.08MetCys: 0.08 ± 0.07
1.757MetAsp: 1.757 ± 0.338
2.157MetGlu: 2.157 ± 0.446
1.039MetPhe: 1.039 ± 0.271
0.959MetGly: 0.959 ± 0.247
0.399MetHis: 0.399 ± 0.172
2.157MetIle: 2.157 ± 0.445
2.476MetLys: 2.476 ± 0.438
1.598MetLeu: 1.598 ± 0.304
0.879MetMet: 0.879 ± 0.233
1.278MetAsn: 1.278 ± 0.303
0.559MetPro: 0.559 ± 0.194
0.719MetGln: 0.719 ± 0.25
0.959MetArg: 0.959 ± 0.315
1.917MetSer: 1.917 ± 0.426
2.237MetThr: 2.237 ± 0.412
1.039MetVal: 1.039 ± 0.255
0.24MetTrp: 0.24 ± 0.11
0.959MetTyr: 0.959 ± 0.269
0.0MetXaa: 0.0 ± 0.0
Asn
4.314AsnAla: 4.314 ± 0.75
0.32AsnCys: 0.32 ± 0.157
4.873AsnAsp: 4.873 ± 0.78
4.953AsnGlu: 4.953 ± 0.668
1.757AsnPhe: 1.757 ± 0.268
4.553AsnGly: 4.553 ± 0.681
0.879AsnHis: 0.879 ± 0.25
3.036AsnIle: 3.036 ± 0.386
7.749AsnLys: 7.749 ± 0.725
4.873AsnLeu: 4.873 ± 0.694
1.438AsnMet: 1.438 ± 0.328
4.873AsnAsn: 4.873 ± 0.805
2.157AsnPro: 2.157 ± 0.404
1.837AsnGln: 1.837 ± 0.411
2.476AsnArg: 2.476 ± 0.468
4.474AsnSer: 4.474 ± 0.646
3.914AsnThr: 3.914 ± 0.542
3.994AsnVal: 3.994 ± 0.566
1.039AsnTrp: 1.039 ± 0.261
3.116AsnTyr: 3.116 ± 0.603
0.0AsnXaa: 0.0 ± 0.0
Pro
1.198ProAla: 1.198 ± 0.373
0.24ProCys: 0.24 ± 0.146
1.039ProAsp: 1.039 ± 0.342
1.358ProGlu: 1.358 ± 0.376
1.198ProPhe: 1.198 ± 0.294
0.639ProGly: 0.639 ± 0.281
0.639ProHis: 0.639 ± 0.228
1.917ProIle: 1.917 ± 0.528
3.595ProLys: 3.595 ± 0.737
1.438ProLeu: 1.438 ± 0.318
0.799ProMet: 0.799 ± 0.211
1.917ProAsn: 1.917 ± 0.315
0.719ProPro: 0.719 ± 0.242
0.959ProGln: 0.959 ± 0.302
0.879ProArg: 0.879 ± 0.32
2.317ProSer: 2.317 ± 0.488
1.837ProThr: 1.837 ± 0.337
1.678ProVal: 1.678 ± 0.409
0.0ProTrp: 0.0 ± 0.0
0.879ProTyr: 0.879 ± 0.225
0.0ProXaa: 0.0 ± 0.0
Gln
2.636GlnAla: 2.636 ± 0.463
0.32GlnCys: 0.32 ± 0.158
1.837GlnAsp: 1.837 ± 0.376
2.077GlnGlu: 2.077 ± 0.5
1.518GlnPhe: 1.518 ± 0.335
1.278GlnGly: 1.278 ± 0.326
0.639GlnHis: 0.639 ± 0.208
2.556GlnIle: 2.556 ± 0.417
4.314GlnLys: 4.314 ± 0.577
4.074GlnLeu: 4.074 ± 0.711
0.559GlnMet: 0.559 ± 0.22
2.876GlnAsn: 2.876 ± 0.535
0.559GlnPro: 0.559 ± 0.198
1.678GlnGln: 1.678 ± 0.385
1.757GlnArg: 1.757 ± 0.332
1.997GlnSer: 1.997 ± 0.357
2.636GlnThr: 2.636 ± 0.544
2.716GlnVal: 2.716 ± 0.367
0.16GlnTrp: 0.16 ± 0.099
1.598GlnTyr: 1.598 ± 0.359
0.0GlnXaa: 0.0 ± 0.0
Arg
1.997ArgAla: 1.997 ± 0.447
0.16ArgCys: 0.16 ± 0.104
2.077ArgAsp: 2.077 ± 0.526
2.317ArgGlu: 2.317 ± 0.451
2.077ArgPhe: 2.077 ± 0.336
1.917ArgGly: 1.917 ± 0.421
1.198ArgHis: 1.198 ± 0.377
3.515ArgIle: 3.515 ± 0.537
2.876ArgLys: 2.876 ± 0.498
4.154ArgLeu: 4.154 ± 0.545
1.118ArgMet: 1.118 ± 0.287
1.997ArgAsn: 1.997 ± 0.288
1.118ArgPro: 1.118 ± 0.384
1.917ArgGln: 1.917 ± 0.375
1.278ArgArg: 1.278 ± 0.321
2.077ArgSer: 2.077 ± 0.378
1.837ArgThr: 1.837 ± 0.498
2.397ArgVal: 2.397 ± 0.422
0.719ArgTrp: 0.719 ± 0.211
2.397ArgTyr: 2.397 ± 0.33
0.0ArgXaa: 0.0 ± 0.0
Ser
3.675SerAla: 3.675 ± 0.753
0.24SerCys: 0.24 ± 0.139
3.755SerAsp: 3.755 ± 0.519
4.953SerGlu: 4.953 ± 0.519
2.397SerPhe: 2.397 ± 0.499
3.755SerGly: 3.755 ± 0.402
1.598SerHis: 1.598 ± 0.376
5.911SerIle: 5.911 ± 0.835
5.672SerLys: 5.672 ± 0.628
5.752SerLeu: 5.752 ± 0.691
1.198SerMet: 1.198 ± 0.33
4.474SerAsn: 4.474 ± 0.583
1.118SerPro: 1.118 ± 0.298
1.917SerGln: 1.917 ± 0.38
2.636SerArg: 2.636 ± 0.5
2.876SerSer: 2.876 ± 0.541
3.595SerThr: 3.595 ± 0.524
3.435SerVal: 3.435 ± 0.503
0.479SerTrp: 0.479 ± 0.219
3.036SerTyr: 3.036 ± 0.485
0.0SerXaa: 0.0 ± 0.0
Thr
3.515ThrAla: 3.515 ± 0.493
0.0ThrCys: 0.0 ± 0.0
3.515ThrAsp: 3.515 ± 0.494
4.074ThrGlu: 4.074 ± 0.621
2.397ThrPhe: 2.397 ± 0.486
3.195ThrGly: 3.195 ± 0.606
1.278ThrHis: 1.278 ± 0.311
5.272ThrIle: 5.272 ± 0.807
6.151ThrLys: 6.151 ± 0.635
5.272ThrLeu: 5.272 ± 0.542
1.278ThrMet: 1.278 ± 0.34
2.956ThrAsn: 2.956 ± 0.568
1.757ThrPro: 1.757 ± 0.355
2.237ThrGln: 2.237 ± 0.457
1.997ThrArg: 1.997 ± 0.366
3.675ThrSer: 3.675 ± 0.579
4.074ThrThr: 4.074 ± 0.626
4.314ThrVal: 4.314 ± 0.693
0.719ThrTrp: 0.719 ± 0.227
2.476ThrTyr: 2.476 ± 0.446
0.0ThrXaa: 0.0 ± 0.0
Val
4.474ValAla: 4.474 ± 0.514
0.24ValCys: 0.24 ± 0.136
5.113ValAsp: 5.113 ± 0.506
5.272ValGlu: 5.272 ± 0.656
2.317ValPhe: 2.317 ± 0.46
3.036ValGly: 3.036 ± 0.59
0.639ValHis: 0.639 ± 0.181
4.553ValIle: 4.553 ± 0.558
5.352ValLys: 5.352 ± 0.618
4.793ValLeu: 4.793 ± 0.592
1.358ValMet: 1.358 ± 0.32
4.633ValAsn: 4.633 ± 0.603
2.237ValPro: 2.237 ± 0.369
1.997ValGln: 1.997 ± 0.339
1.757ValArg: 1.757 ± 0.339
5.512ValSer: 5.512 ± 0.616
3.675ValThr: 3.675 ± 0.62
4.074ValVal: 4.074 ± 0.602
0.719ValTrp: 0.719 ± 0.228
2.397ValTyr: 2.397 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
0.799TrpAla: 0.799 ± 0.299
0.08TrpCys: 0.08 ± 0.071
0.559TrpAsp: 0.559 ± 0.204
0.24TrpGlu: 0.24 ± 0.128
0.399TrpPhe: 0.399 ± 0.188
0.32TrpGly: 0.32 ± 0.209
0.08TrpHis: 0.08 ± 0.071
0.479TrpIle: 0.479 ± 0.178
0.639TrpLys: 0.639 ± 0.22
0.719TrpLeu: 0.719 ± 0.201
0.16TrpMet: 0.16 ± 0.112
1.278TrpAsn: 1.278 ± 0.516
0.16TrpPro: 0.16 ± 0.118
0.399TrpGln: 0.399 ± 0.203
0.399TrpArg: 0.399 ± 0.16
1.278TrpSer: 1.278 ± 0.33
0.879TrpThr: 0.879 ± 0.261
0.719TrpVal: 0.719 ± 0.224
0.0TrpTrp: 0.0 ± 0.0
0.719TrpTyr: 0.719 ± 0.254
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.317TyrAla: 2.317 ± 0.33
0.16TyrCys: 0.16 ± 0.119
3.036TyrAsp: 3.036 ± 0.486
2.716TyrGlu: 2.716 ± 0.483
2.317TyrPhe: 2.317 ± 0.561
2.157TyrGly: 2.157 ± 0.428
0.959TyrHis: 0.959 ± 0.325
2.876TyrIle: 2.876 ± 0.558
4.873TyrLys: 4.873 ± 0.631
3.036TyrLeu: 3.036 ± 0.481
1.118TyrMet: 1.118 ± 0.289
2.956TyrAsn: 2.956 ± 0.463
1.278TyrPro: 1.278 ± 0.342
2.077TyrGln: 2.077 ± 0.371
2.397TyrArg: 2.397 ± 0.508
2.796TyrSer: 2.796 ± 0.477
2.317TyrThr: 2.317 ± 0.424
2.317TyrVal: 2.317 ± 0.428
0.879TyrTrp: 0.879 ± 0.313
2.077TyrTyr: 2.077 ± 0.425
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12519 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski