Notothenia coriiceps (black rockcod)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata;

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I9N4H7|A0A6I9N4H7_9TELE trafficking protein particle complex subunit 10 OS=Notothenia coriiceps OX=8208 GN=trappc10 PE=4 SV=1
MM1 pKa = 7.51SPLFIVQQTGLVQLQSLVNSCSTSDD26 pKa = 3.57NQTSSQNNMTVDD38 pKa = 3.51MNTASSLQQYY48 pKa = 9.52RR49 pKa = 11.84SRR51 pKa = 11.84IGSSANQSPTSPVSNQGFSPGGSPQHH77 pKa = 6.1NSILGSVFADD87 pKa = 5.57FYY89 pKa = 11.38DD90 pKa = 3.5QQLPSIQASALSQQLEE106 pKa = 4.39QFNMMEE112 pKa = 4.52TPISSDD118 pKa = 2.74SLYY121 pKa = 11.11NQTSTLNYY129 pKa = 9.74SQALMMGLTGHH140 pKa = 6.96CNLQDD145 pKa = 3.89SQQLGYY151 pKa = 10.13SSHH154 pKa = 6.69GNIPNIILTVSGEE167 pKa = 4.41SPPSLPKK174 pKa = 10.74DD175 pKa = 3.36LTGCLSTDD183 pKa = 2.93VSFDD187 pKa = 3.08IDD189 pKa = 3.84TQFPLDD195 pKa = 4.35DD196 pKa = 4.83LKK198 pKa = 10.77IDD200 pKa = 4.14PLTLDD205 pKa = 4.33GLHH208 pKa = 6.4MLNDD212 pKa = 4.13PDD214 pKa = 5.58MILTDD219 pKa = 4.37PATEE223 pKa = 3.95DD224 pKa = 3.1AFRR227 pKa = 11.84LDD229 pKa = 3.51RR230 pKa = 11.84LL231 pKa = 3.74

Molecular weight: 25.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I9PLI8|A0A6I9PLI8_9TELE Isoform of A0A6I9PMS2 uncharacterized protein LOC104961826 isoform X2 OS=Notothenia coriiceps OX=8208 GN=LOC104961826 PE=4 SV=1
MM1 pKa = 7.56SAPSSQKK8 pKa = 10.15VVLKK12 pKa = 9.94STTKK16 pKa = 9.76VSLNEE21 pKa = 4.01RR22 pKa = 11.84FTNMLKK28 pKa = 10.37NKK30 pKa = 9.57QPAAVSIRR38 pKa = 11.84ATMQQQHH45 pKa = 6.32LASARR50 pKa = 11.84NRR52 pKa = 11.84RR53 pKa = 11.84LAQQMEE59 pKa = 4.32NRR61 pKa = 11.84PSVQAALNHH70 pKa = 5.68KK71 pKa = 10.23QSLKK75 pKa = 10.22QRR77 pKa = 11.84LGKK80 pKa = 10.77SNIQARR86 pKa = 11.84LGRR89 pKa = 11.84PVGALMRR96 pKa = 11.84GGAPGSRR103 pKa = 11.84GGMRR107 pKa = 11.84GMNRR111 pKa = 11.84GGLRR115 pKa = 11.84GRR117 pKa = 11.84ARR119 pKa = 11.84GGIMRR124 pKa = 11.84GALSLRR130 pKa = 11.84GKK132 pKa = 10.08RR133 pKa = 11.84VASTGGPMRR142 pKa = 11.84GRR144 pKa = 11.84GSAGRR149 pKa = 11.84LAMRR153 pKa = 11.84RR154 pKa = 11.84AGRR157 pKa = 11.84NRR159 pKa = 11.84GGPVGRR165 pKa = 11.84GGALSRR171 pKa = 11.84GAARR175 pKa = 11.84GAARR179 pKa = 11.84GGVARR184 pKa = 11.84GG185 pKa = 3.26

Molecular weight: 19.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24776

5376

30152

14412374

37

7994

478.0

53.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.565 ± 0.013

2.123 ± 0.013

5.206 ± 0.01

7.08 ± 0.021

3.416 ± 0.01

6.536 ± 0.027

2.647 ± 0.008

4.178 ± 0.01

5.658 ± 0.016

9.247 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.006

3.809 ± 0.008

6.035 ± 0.019

4.864 ± 0.015

5.672 ± 0.012

9.046 ± 0.021

5.645 ± 0.012

6.156 ± 0.012

1.075 ± 0.005

2.594 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski