Anaerocolumna xylanovorans DSM 12503

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerocolumna; Anaerocolumna xylanovorans

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4651 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M7YFW8|A0A1M7YFW8_9FIRM Raffinose/stachyose/melibiose transport system permease protein OS=Anaerocolumna xylanovorans DSM 12503 OX=1121345 GN=SAMN02745217_03224 PE=3 SV=1
MM1 pKa = 7.21KK2 pKa = 9.93RR3 pKa = 11.84VKK5 pKa = 10.55IMMTLLIPALLFSACNGADD24 pKa = 3.42RR25 pKa = 11.84SSTASDD31 pKa = 3.76PSALQSMLTEE41 pKa = 4.26QIPDD45 pKa = 3.68DD46 pKa = 3.91FGFTYY51 pKa = 9.76IHH53 pKa = 6.53NFIEE57 pKa = 4.1VDD59 pKa = 3.68TFNSTIKK66 pKa = 8.43NTYY69 pKa = 8.69YY70 pKa = 10.62FSEE73 pKa = 4.76GDD75 pKa = 3.4PDD77 pKa = 5.46AITDD81 pKa = 3.77FSFSHH86 pKa = 5.9EE87 pKa = 4.09QLEE90 pKa = 4.54KK91 pKa = 10.07IYY93 pKa = 10.57EE94 pKa = 3.92AFIQYY99 pKa = 10.0DD100 pKa = 3.49IQDD103 pKa = 4.26LPEE106 pKa = 5.14EE107 pKa = 4.42IDD109 pKa = 3.45PDD111 pKa = 3.83KK112 pKa = 11.46GFMSIPPSYY121 pKa = 11.2YY122 pKa = 8.94EE123 pKa = 4.5LLYY126 pKa = 10.69ISDD129 pKa = 4.62GEE131 pKa = 4.54TKK133 pKa = 10.58NISCRR138 pKa = 11.84TGADD142 pKa = 2.91IDD144 pKa = 3.81QEE146 pKa = 4.85GISEE150 pKa = 4.31DD151 pKa = 3.33NNNFVKK157 pKa = 10.65FMAEE161 pKa = 3.33VWNYY165 pKa = 10.23VYY167 pKa = 10.74EE168 pKa = 4.47SDD170 pKa = 4.21AYY172 pKa = 10.6QRR174 pKa = 11.84LPSGPEE180 pKa = 3.58TEE182 pKa = 4.21

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7Y9E8|A0A1M7Y9E8_9FIRM Tetratricopeptide repeat-containing protein OS=Anaerocolumna xylanovorans DSM 12503 OX=1121345 GN=SAMN02745217_02224 PE=4 SV=1
MM1 pKa = 8.0AYY3 pKa = 10.48NKK5 pKa = 9.96NDD7 pKa = 3.6RR8 pKa = 11.84NSNRR12 pKa = 11.84DD13 pKa = 3.52DD14 pKa = 4.36KK15 pKa = 11.57GDD17 pKa = 3.42SPMRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84NTRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.39KK30 pKa = 10.24VCAFCADD37 pKa = 3.9KK38 pKa = 11.2NHH40 pKa = 6.6TGIDD44 pKa = 3.6YY45 pKa = 11.12KK46 pKa = 11.13DD47 pKa = 3.47VNKK50 pKa = 10.54LKK52 pKa = 10.45RR53 pKa = 11.84YY54 pKa = 7.87VSEE57 pKa = 4.16RR58 pKa = 11.84GKK60 pKa = 10.18ILPRR64 pKa = 11.84RR65 pKa = 11.84ITGNCAKK72 pKa = 9.81HH73 pKa = 5.44QRR75 pKa = 11.84ALTVAIKK82 pKa = 10.11RR83 pKa = 11.84ARR85 pKa = 11.84HH86 pKa = 5.13IALMPYY92 pKa = 9.53TLDD95 pKa = 3.28

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4651

0

4651

1598021

39

4409

343.6

38.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.913 ± 0.037

1.268 ± 0.014

5.429 ± 0.024

7.363 ± 0.044

4.244 ± 0.029

6.894 ± 0.033

1.564 ± 0.014

8.133 ± 0.032

7.532 ± 0.034

9.312 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.016

4.981 ± 0.026

3.152 ± 0.02

2.924 ± 0.018

3.875 ± 0.027

6.289 ± 0.032

5.608 ± 0.04

6.339 ± 0.028

0.93 ± 0.013

4.482 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski