Actinoplanes atraurantiacus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285ICT6|A0A285ICT6_9ACTN Histidine triad (HIT) family protein OS=Actinoplanes atraurantiacus OX=1036182 GN=SAMN05421748_107257 PE=4 SV=1
MM1 pKa = 7.68TDD3 pKa = 2.63HH4 pKa = 7.43HH5 pKa = 7.39DD6 pKa = 4.22FGDD9 pKa = 3.94HH10 pKa = 6.82EE11 pKa = 5.46DD12 pKa = 3.99DD13 pKa = 5.04FGVFDD18 pKa = 3.97DD19 pKa = 4.5HH20 pKa = 7.04QLPEE24 pKa = 4.27HH25 pKa = 6.86AEE27 pKa = 3.8PLPFEE32 pKa = 4.39DD33 pKa = 3.31HH34 pKa = 7.09FEE36 pKa = 4.43YY37 pKa = 10.8EE38 pKa = 4.29VDD40 pKa = 3.49DD41 pKa = 3.55HH42 pKa = 6.46HH43 pKa = 7.3TYY45 pKa = 10.9DD46 pKa = 4.01EE47 pKa = 4.77PVHH50 pKa = 6.44EE51 pKa = 4.63PAEE54 pKa = 4.35HH55 pKa = 6.45VFDD58 pKa = 4.29EE59 pKa = 5.0PEE61 pKa = 4.7HH62 pKa = 6.11EE63 pKa = 4.56VAEE66 pKa = 4.65LPADD70 pKa = 3.96DD71 pKa = 5.01PVDD74 pKa = 3.7VFPPPVHH81 pKa = 7.36VGEE84 pKa = 4.61LPEE87 pKa = 4.54PVDD90 pKa = 3.63GFPWIDD96 pKa = 3.28TGSLGVVPADD106 pKa = 3.41ALVTPVDD113 pKa = 3.51PVQPQEE119 pKa = 3.9LADD122 pKa = 3.8YY123 pKa = 10.57AGEE126 pKa = 3.92QLPPGVDD133 pKa = 3.14PWAALADD140 pKa = 4.08SEE142 pKa = 5.58DD143 pKa = 4.11PATSTLARR151 pKa = 11.84WWQEE155 pKa = 3.5NN156 pKa = 3.32

Molecular weight: 17.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285FV58|A0A285FV58_9ACTN Uncharacterized protein OS=Actinoplanes atraurantiacus OX=1036182 GN=SAMN05421748_1011287 PE=4 SV=1
MM1 pKa = 7.05VPSRR5 pKa = 11.84QVPLRR10 pKa = 11.84RR11 pKa = 11.84LRR13 pKa = 11.84PRR15 pKa = 11.84PRR17 pKa = 11.84PPPAVLRR24 pKa = 11.84PARR27 pKa = 11.84RR28 pKa = 11.84HH29 pKa = 4.71RR30 pKa = 11.84LARR33 pKa = 11.84LRR35 pKa = 11.84PPRR38 pKa = 11.84VPSRR42 pKa = 11.84RR43 pKa = 11.84AGPAPPPVALPAPRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84PPRR63 pKa = 11.84RR64 pKa = 11.84PALSRR69 pKa = 11.84VPSRR73 pKa = 11.84RR74 pKa = 11.84FPAVRR79 pKa = 11.84APRR82 pKa = 11.84PARR85 pKa = 11.84SPSRR89 pKa = 11.84PAPTTSRR96 pKa = 11.84SRR98 pKa = 11.84RR99 pKa = 11.84PRR101 pKa = 11.84PVPRR105 pKa = 11.84RR106 pKa = 11.84SRR108 pKa = 11.84PSRR111 pKa = 11.84RR112 pKa = 11.84PRR114 pKa = 11.84SRR116 pKa = 11.84PLRR119 pKa = 11.84PPARR123 pKa = 11.84VTPSAGRR130 pKa = 11.84SSPRR134 pKa = 11.84PTAARR139 pKa = 11.84PVAPDD144 pKa = 3.92RR145 pKa = 11.84VPAPARR151 pKa = 11.84FRR153 pKa = 11.84RR154 pKa = 11.84ARR156 pKa = 11.84VRR158 pKa = 11.84PRR160 pKa = 11.84PAPPRR165 pKa = 11.84RR166 pKa = 11.84VAAVLRR172 pKa = 11.84PARR175 pKa = 11.84VPAPPVATPRR185 pKa = 11.84ARR187 pKa = 11.84RR188 pKa = 11.84RR189 pKa = 11.84VARR192 pKa = 11.84VTTRR196 pKa = 11.84SASRR200 pKa = 11.84VATPPVPRR208 pKa = 11.84RR209 pKa = 11.84LPCPGPTSRR218 pKa = 11.84ACPRR222 pKa = 11.84GRR224 pKa = 11.84ARR226 pKa = 11.84PRR228 pKa = 11.84CPRR231 pKa = 11.84GPAPRR236 pKa = 11.84PCPRR240 pKa = 11.84SVRR243 pKa = 11.84PAPAVRR249 pKa = 11.84VAAPVAVAAPVAAAVVAVPVAASVPVAAAVPVAASVPVAAAVPVVAVATAVVPAAAVAVATAAVPVVAAVLRR321 pKa = 11.84PVPALRR327 pKa = 11.84VVPVAAVAVVAAVPRR342 pKa = 11.84VPSGVRR348 pKa = 11.84AAGRR352 pKa = 11.84PAVASRR358 pKa = 11.84RR359 pKa = 11.84SSGVKK364 pKa = 10.17SSTTCPPRR372 pKa = 11.84RR373 pKa = 3.6

Molecular weight: 39.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10240

0

10240

3323307

24

7153

324.5

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.908 ± 0.039

0.665 ± 0.006

5.969 ± 0.021

5.221 ± 0.025

2.834 ± 0.015

9.2 ± 0.025

2.064 ± 0.012

3.664 ± 0.017

2.066 ± 0.018

10.334 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.734 ± 0.009

1.968 ± 0.016

6.06 ± 0.022

2.771 ± 0.014

7.857 ± 0.03

5.27 ± 0.022

6.18 ± 0.027

8.522 ± 0.023

1.593 ± 0.01

2.121 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski