Amino acid dipepetide frequency for Brevundimonas sp. MYb31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.874AlaAla: 20.874 ± 0.222	1.131AlaCys: 1.131 ± 0.036	8.063AlaAsp: 8.063 ± 0.097	8.498AlaGlu: 8.498 ± 0.107	4.91AlaPhe: 4.91 ± 0.068	11.513AlaGly: 11.513 ± 0.131	2.29AlaHis: 2.29 ± 0.052	5.41AlaIle: 5.41 ± 0.086	3.76AlaLys: 3.76 ± 0.071	14.617AlaLeu: 14.617 ± 0.144	3.487AlaMet: 3.487 ± 0.068	2.873AlaAsn: 2.873 ± 0.062	7.518AlaPro: 7.518 ± 0.108	4.449AlaGln: 4.449 ± 0.065	10.183AlaArg: 10.183 ± 0.107	6.984AlaSer: 6.984 ± 0.098	6.167AlaThr: 6.167 ± 0.086	10.692AlaVal: 10.692 ± 0.119	1.972AlaTrp: 1.972 ± 0.05	2.854AlaTyr: 2.854 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.97CysAla: 0.97 ± 0.034	0.068CysCys: 0.068 ± 0.009	0.44CysAsp: 0.44 ± 0.022	0.371CysGlu: 0.371 ± 0.019	0.239CysPhe: 0.239 ± 0.015	0.775CysGly: 0.775 ± 0.029	0.15CysHis: 0.15 ± 0.011	0.286CysIle: 0.286 ± 0.016	0.154CysLys: 0.154 ± 0.012	0.645CysLeu: 0.645 ± 0.025	0.114CysMet: 0.114 ± 0.012	0.175CysAsn: 0.175 ± 0.013	0.379CysPro: 0.379 ± 0.021	0.169CysGln: 0.169 ± 0.013	0.483CysArg: 0.483 ± 0.021	0.362CysSer: 0.362 ± 0.022	0.317CysThr: 0.317 ± 0.018	0.525CysVal: 0.525 ± 0.023	0.095CysTrp: 0.095 ± 0.009	0.132CysTyr: 0.132 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.206AspAla: 8.206 ± 0.098	0.413AspCys: 0.413 ± 0.02	3.338AspAsp: 3.338 ± 0.074	3.429AspGlu: 3.429 ± 0.07	2.162AspPhe: 2.162 ± 0.054	5.68AspGly: 5.68 ± 0.097	1.363AspHis: 1.363 ± 0.043	2.569AspIle: 2.569 ± 0.055	1.613AspLys: 1.613 ± 0.046	6.504AspLeu: 6.504 ± 0.091	1.375AspMet: 1.375 ± 0.035	1.264AspAsn: 1.264 ± 0.038	3.644AspPro: 3.644 ± 0.063	2.147AspGln: 2.147 ± 0.046	4.765AspArg: 4.765 ± 0.077	2.144AspSer: 2.144 ± 0.051	2.345AspThr: 2.345 ± 0.047	4.151AspVal: 4.151 ± 0.058	1.144AspTrp: 1.144 ± 0.033	1.599AspTyr: 1.599 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	9.507GluAla: 9.507 ± 0.114	0.245GluCys: 0.245 ± 0.015	2.843GluAsp: 2.843 ± 0.062	2.615GluGlu: 2.615 ± 0.058	1.494GluPhe: 1.494 ± 0.042	5.061GluGly: 5.061 ± 0.082	1.056GluHis: 1.056 ± 0.033	2.872GluIle: 2.872 ± 0.064	1.744GluLys: 1.744 ± 0.045	4.716GluLeu: 4.716 ± 0.074	1.312GluMet: 1.312 ± 0.038	1.265GluAsn: 1.265 ± 0.035	2.703GluPro: 2.703 ± 0.056	2.18GluGln: 2.18 ± 0.051	4.88GluArg: 4.88 ± 0.076	2.049GluSer: 2.049 ± 0.042	3.674GluThr: 3.674 ± 0.066	4.191GluVal: 4.191 ± 0.064	0.716GluTrp: 0.716 ± 0.027	0.93GluTyr: 0.93 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.531PheAla: 4.531 ± 0.075	0.297PheCys: 0.297 ± 0.017	2.803PheAsp: 2.803 ± 0.048	2.118PheGlu: 2.118 ± 0.042	1.181PhePhe: 1.181 ± 0.035	3.545PheGly: 3.545 ± 0.059	0.688PheHis: 0.688 ± 0.025	1.534PheIle: 1.534 ± 0.039	0.933PheLys: 0.933 ± 0.035	2.9PheLeu: 2.9 ± 0.06	0.798PheMet: 0.798 ± 0.028	1.105PheAsn: 1.105 ± 0.03	1.316PhePro: 1.316 ± 0.038	1.082PheGln: 1.082 ± 0.033	2.093PheArg: 2.093 ± 0.047	1.961PheSer: 1.961 ± 0.043	2.002PheThr: 2.002 ± 0.045	2.683PheVal: 2.683 ± 0.047	0.563PheTrp: 0.563 ± 0.026	0.82PheTyr: 0.82 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	11.445GlyAla: 11.445 ± 0.132	0.696GlyCys: 0.696 ± 0.027	5.107GlyAsp: 5.107 ± 0.083	5.15GlyGlu: 5.15 ± 0.07	3.628GlyPhe: 3.628 ± 0.059	8.405GlyGly: 8.405 ± 0.116	1.651GlyHis: 1.651 ± 0.04	2.931GlyIle: 2.931 ± 0.058	2.8GlyLys: 2.8 ± 0.056	9.676GlyLeu: 9.676 ± 0.117	2.177GlyMet: 2.177 ± 0.049	1.606GlyAsn: 1.606 ± 0.046	3.901GlyPro: 3.901 ± 0.066	3.401GlyGln: 3.401 ± 0.065	6.993GlyArg: 6.993 ± 0.081	4.4GlySer: 4.4 ± 0.074	3.399GlyThr: 3.399 ± 0.071	8.091GlyVal: 8.091 ± 0.1	1.668GlyTrp: 1.668 ± 0.042	2.223GlyTyr: 2.223 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	2.34HisAla: 2.34 ± 0.05	0.149HisCys: 0.149 ± 0.013	1.174HisAsp: 1.174 ± 0.035	1.054HisGlu: 1.054 ± 0.032	0.659HisPhe: 0.659 ± 0.025	1.814HisGly: 1.814 ± 0.049	0.437HisHis: 0.437 ± 0.023	0.685HisIle: 0.685 ± 0.027	0.396HisLys: 0.396 ± 0.023	1.759HisLeu: 1.759 ± 0.041	0.412HisMet: 0.412 ± 0.02	0.417HisAsn: 0.417 ± 0.021	1.259HisPro: 1.259 ± 0.042	0.559HisGln: 0.559 ± 0.023	1.272HisArg: 1.272 ± 0.037	0.75HisSer: 0.75 ± 0.027	0.724HisThr: 0.724 ± 0.027	1.407HisVal: 1.407 ± 0.041	0.286HisTrp: 0.286 ± 0.017	0.452HisTyr: 0.452 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.296IleAla: 6.296 ± 0.087	0.358IleCys: 0.358 ± 0.019	3.336IleAsp: 3.336 ± 0.055	3.3IleGlu: 3.3 ± 0.063	1.285IlePhe: 1.285 ± 0.037	4.382IleGly: 4.382 ± 0.071	0.799IleHis: 0.799 ± 0.027	1.774IleIle: 1.774 ± 0.046	1.236IleLys: 1.236 ± 0.04	3.9IleLeu: 3.9 ± 0.077	0.841IleMet: 0.841 ± 0.029	1.289IleAsn: 1.289 ± 0.035	1.952IlePro: 1.952 ± 0.05	1.298IleGln: 1.298 ± 0.039	2.849IleArg: 2.849 ± 0.061	2.31IleSer: 2.31 ± 0.054	2.324IleThr: 2.324 ± 0.056	3.524IleVal: 3.524 ± 0.058	0.533IleTrp: 0.533 ± 0.026	0.856IleTyr: 0.856 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.515LysAla: 4.515 ± 0.08	0.095LysCys: 0.095 ± 0.009	1.582LysAsp: 1.582 ± 0.043	1.139LysGlu: 1.139 ± 0.04	0.762LysPhe: 0.762 ± 0.027	2.627LysGly: 2.627 ± 0.059	0.5LysHis: 0.5 ± 0.021	1.345LysIle: 1.345 ± 0.043	1.098LysLys: 1.098 ± 0.039	2.721LysLeu: 2.721 ± 0.065	0.629LysMet: 0.629 ± 0.024	0.708LysAsn: 0.708 ± 0.029	1.902LysPro: 1.902 ± 0.044	0.808LysGln: 0.808 ± 0.034	2.112LysArg: 2.112 ± 0.054	1.515LysSer: 1.515 ± 0.045	2.027LysThr: 2.027 ± 0.05	2.146LysVal: 2.146 ± 0.049	0.341LysTrp: 0.341 ± 0.018	0.518LysTyr: 0.518 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.797LeuAla: 13.797 ± 0.127	0.669LeuCys: 0.669 ± 0.026	6.226LeuAsp: 6.226 ± 0.088	5.177LeuGlu: 5.177 ± 0.084	3.512LeuPhe: 3.512 ± 0.063	8.221LeuGly: 8.221 ± 0.1	1.687LeuHis: 1.687 ± 0.05	5.337LeuIle: 5.337 ± 0.089	3.854LeuLys: 3.854 ± 0.068	8.703LeuLeu: 8.703 ± 0.123	2.387LeuMet: 2.387 ± 0.051	3.005LeuAsn: 3.005 ± 0.054	5.239LeuPro: 5.239 ± 0.077	2.67LeuGln: 2.67 ± 0.054	6.24LeuArg: 6.24 ± 0.089	6.472LeuSer: 6.472 ± 0.088	6.652LeuThr: 6.652 ± 0.093	6.502LeuVal: 6.502 ± 0.088	1.262LeuTrp: 1.262 ± 0.04	1.999LeuTyr: 1.999 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	3.12MetAla: 3.12 ± 0.055	0.098MetCys: 0.098 ± 0.01	1.203MetAsp: 1.203 ± 0.033	0.978MetGlu: 0.978 ± 0.032	0.641MetPhe: 0.641 ± 0.029	1.997MetGly: 1.997 ± 0.044	0.375MetHis: 0.375 ± 0.02	1.282MetIle: 1.282 ± 0.038	0.95MetLys: 0.95 ± 0.027	2.117MetLeu: 2.117 ± 0.049	0.558MetMet: 0.558 ± 0.028	0.737MetAsn: 0.737 ± 0.028	1.163MetPro: 1.163 ± 0.037	0.779MetGln: 0.779 ± 0.028	1.69MetArg: 1.69 ± 0.044	1.523MetSer: 1.523 ± 0.041	2.019MetThr: 2.019 ± 0.047	1.454MetVal: 1.454 ± 0.045	0.214MetTrp: 0.214 ± 0.013	0.245MetTyr: 0.245 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.274AsnAla: 3.274 ± 0.061	0.179AsnCys: 0.179 ± 0.014	1.398AsnAsp: 1.398 ± 0.039	1.114AsnGlu: 1.114 ± 0.034	0.791AsnPhe: 0.791 ± 0.029	2.364AsnGly: 2.364 ± 0.047	0.471AsnHis: 0.471 ± 0.023	1.149AsnIle: 1.149 ± 0.04	0.546AsnLys: 0.546 ± 0.022	2.6AsnLeu: 2.6 ± 0.059	0.514AsnMet: 0.514 ± 0.024	0.634AsnAsn: 0.634 ± 0.029	1.758AsnPro: 1.758 ± 0.041	0.747AsnGln: 0.747 ± 0.029	1.796AsnArg: 1.796 ± 0.044	1.072AsnSer: 1.072 ± 0.042	1.217AsnThr: 1.217 ± 0.043	1.855AsnVal: 1.855 ± 0.047	0.391AsnTrp: 0.391 ± 0.019	0.6AsnTyr: 0.6 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.255ProAla: 7.255 ± 0.103	0.306ProCys: 0.306 ± 0.016	3.802ProAsp: 3.802 ± 0.06	3.731ProGlu: 3.731 ± 0.06	1.923ProPhe: 1.923 ± 0.044	4.863ProGly: 4.863 ± 0.069	0.967ProHis: 0.967 ± 0.029	2.278ProIle: 2.278 ± 0.046	1.438ProLys: 1.438 ± 0.038	4.893ProLeu: 4.893 ± 0.08	1.149ProMet: 1.149 ± 0.033	1.373ProAsn: 1.373 ± 0.035	2.678ProPro: 2.678 ± 0.07	1.682ProGln: 1.682 ± 0.042	3.207ProArg: 3.207 ± 0.066	2.917ProSer: 2.917 ± 0.061	2.877ProThr: 2.877 ± 0.056	4.364ProVal: 4.364 ± 0.067	0.794ProTrp: 0.794 ± 0.031	1.127ProTyr: 1.127 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	5.089GlnAla: 5.089 ± 0.081	0.155GlnCys: 0.155 ± 0.012	1.566GlnAsp: 1.566 ± 0.036	1.342GlnGlu: 1.342 ± 0.043	0.904GlnPhe: 0.904 ± 0.029	2.785GlnGly: 2.785 ± 0.053	0.529GlnHis: 0.529 ± 0.022	1.574GlnIle: 1.574 ± 0.035	1.036GlnLys: 1.036 ± 0.032	2.63GlnLeu: 2.63 ± 0.057	0.839GlnMet: 0.839 ± 0.03	0.805GlnAsn: 0.805 ± 0.026	1.919GlnPro: 1.919 ± 0.04	1.119GlnGln: 1.119 ± 0.042	2.463GlnArg: 2.463 ± 0.049	1.801GlnSer: 1.801 ± 0.044	2.149GlnThr: 2.149 ± 0.05	2.444GlnVal: 2.444 ± 0.056	0.416GlnTrp: 0.416 ± 0.019	0.529GlnTyr: 0.529 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.898ArgAla: 8.898 ± 0.108	0.425ArgCys: 0.425 ± 0.021	4.324ArgAsp: 4.324 ± 0.073	3.886ArgGlu: 3.886 ± 0.066	2.985ArgPhe: 2.985 ± 0.046	5.122ArgGly: 5.122 ± 0.07	1.375ArgHis: 1.375 ± 0.038	3.677ArgIle: 3.677 ± 0.055	1.885ArgLys: 1.885 ± 0.044	8.756ArgLeu: 8.756 ± 0.111	1.888ArgMet: 1.888 ± 0.042	1.645ArgAsn: 1.645 ± 0.039	4.211ArgPro: 4.211 ± 0.072	2.49ArgGln: 2.49 ± 0.05	6.281ArgArg: 6.281 ± 0.121	3.478ArgSer: 3.478 ± 0.055	3.625ArgThr: 3.625 ± 0.056	5.077ArgVal: 5.077 ± 0.078	1.165ArgTrp: 1.165 ± 0.032	1.732ArgTyr: 1.732 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.603SerAla: 6.603 ± 0.073	0.288SerCys: 0.288 ± 0.018	3.106SerAsp: 3.106 ± 0.058	2.601SerGlu: 2.601 ± 0.053	1.881SerPhe: 1.881 ± 0.047	5.349SerGly: 5.349 ± 0.073	0.867SerHis: 0.867 ± 0.034	2.284SerIle: 2.284 ± 0.047	1.347SerLys: 1.347 ± 0.04	5.408SerLeu: 5.408 ± 0.081	1.11SerMet: 1.11 ± 0.035	1.325SerAsn: 1.325 ± 0.037	3.142SerPro: 3.142 ± 0.052	1.57SerGln: 1.57 ± 0.038	3.517SerArg: 3.517 ± 0.058	2.72SerSer: 2.72 ± 0.062	2.647SerThr: 2.647 ± 0.055	3.85SerVal: 3.85 ± 0.069	0.74SerTrp: 0.74 ± 0.026	1.101SerTyr: 1.101 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.144ThrAla: 7.144 ± 0.081	0.352ThrCys: 0.352 ± 0.016	3.043ThrAsp: 3.043 ± 0.053	2.566ThrGlu: 2.566 ± 0.048	1.801ThrPhe: 1.801 ± 0.041	5.298ThrGly: 5.298 ± 0.092	0.929ThrHis: 0.929 ± 0.032	2.194ThrIle: 2.194 ± 0.051	1.257ThrLys: 1.257 ± 0.041	5.823ThrLeu: 5.823 ± 0.075	0.926ThrMet: 0.926 ± 0.031	1.25ThrAsn: 1.25 ± 0.037	4.115ThrPro: 4.115 ± 0.082	1.535ThrGln: 1.535 ± 0.042	3.516ThrArg: 3.516 ± 0.058	2.551ThrSer: 2.551 ± 0.056	2.999ThrThr: 2.999 ± 0.059	4.315ThrVal: 4.315 ± 0.06	0.713ThrTrp: 0.713 ± 0.027	1.202ThrTyr: 1.202 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	10.063ValAla: 10.063 ± 0.127	0.617ValCys: 0.617 ± 0.026	4.241ValAsp: 4.241 ± 0.065	5.128ValGlu: 5.128 ± 0.072	2.847ValPhe: 2.847 ± 0.051	6.198ValGly: 6.198 ± 0.089	1.264ValHis: 1.264 ± 0.04	3.855ValIle: 3.855 ± 0.073	2.085ValLys: 2.085 ± 0.053	7.575ValLeu: 7.575 ± 0.086	1.853ValMet: 1.853 ± 0.043	1.972ValAsn: 1.972 ± 0.057	3.048ValPro: 3.048 ± 0.054	2.297ValGln: 2.297 ± 0.053	5.409ValArg: 5.409 ± 0.085	4.345ValSer: 4.345 ± 0.071	4.471ValThr: 4.471 ± 0.074	6.701ValVal: 6.701 ± 0.095	1.133ValTrp: 1.133 ± 0.035	1.396ValTyr: 1.396 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.59TrpAla: 1.59 ± 0.042	0.115TrpCys: 0.115 ± 0.012	0.737TrpAsp: 0.737 ± 0.026	0.589TrpGlu: 0.589 ± 0.024	0.571TrpPhe: 0.571 ± 0.023	1.107TrpGly: 1.107 ± 0.033	0.234TrpHis: 0.234 ± 0.015	0.723TrpIle: 0.723 ± 0.028	0.473TrpLys: 0.473 ± 0.023	1.721TrpLeu: 1.721 ± 0.043	0.393TrpMet: 0.393 ± 0.019	0.482TrpAsn: 0.482 ± 0.02	0.729TrpPro: 0.729 ± 0.03	0.401TrpGln: 0.401 ± 0.018	1.494TrpArg: 1.494 ± 0.034	1.037TrpSer: 1.037 ± 0.033	0.969TrpThr: 0.969 ± 0.03	0.855TrpVal: 0.855 ± 0.028	0.276TrpTrp: 0.276 ± 0.018	0.24TrpTyr: 0.24 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.729TyrAla: 2.729 ± 0.055	0.194TyrCys: 0.194 ± 0.013	1.525TyrAsp: 1.525 ± 0.038	1.266TyrGlu: 1.266 ± 0.037	0.776TyrPhe: 0.776 ± 0.028	2.244TyrGly: 2.244 ± 0.051	0.374TyrHis: 0.374 ± 0.019	0.682TyrIle: 0.682 ± 0.027	0.484TyrLys: 0.484 ± 0.023	1.951TyrLeu: 1.951 ± 0.042	0.381TyrMet: 0.381 ± 0.021	0.538TyrAsn: 0.538 ± 0.025	1.05TyrPro: 1.05 ± 0.032	0.711TyrGln: 0.711 ± 0.028	1.68TyrArg: 1.68 ± 0.051	1.062TyrSer: 1.062 ± 0.036	0.922TyrThr: 0.922 ± 0.039	1.669TyrVal: 1.669 ± 0.047	0.315TyrTrp: 0.315 ± 0.019	0.524TyrTyr: 0.524 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3210 proteins (1021726 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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