Dielma fastidiosa
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3467 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A318L5U5|A0A318L5U5_9FIRM Putative ABC transport system ATP-binding protein OS=Dielma fastidiosa OX=1034346 GN=DES51_1126 PE=4 SV=1
MM1 pKa = 6.99 FAAIIGKK8 pKa = 9.8 GKK10 pKa = 10.26 LVLLIFCLLSSMPMHH25 pKa = 6.57 SFLEE29 pKa = 4.11 IEE31 pKa = 4.22 EE32 pKa = 4.47 WEE34 pKa = 4.27 DD35 pKa = 3.02 TGNEE39 pKa = 3.97 VLEE42 pKa = 4.92 DD43 pKa = 3.51 EE44 pKa = 5.39 GEE46 pKa = 4.47 SADD49 pKa = 4.96 HH50 pKa = 6.81 EE51 pKa = 4.77 SADD54 pKa = 3.99 EE55 pKa = 4.46 DD56 pKa = 4.21 SDD58 pKa = 4.17 YY59 pKa = 11.73 QEE61 pKa = 5.09 ADD63 pKa = 2.84 QGEE66 pKa = 4.65 DD67 pKa = 3.62 LNEE70 pKa = 3.94 ATAGDD75 pKa = 4.38 ILPDD79 pKa = 3.72 DD80 pKa = 4.17 TASDD84 pKa = 4.13 SDD86 pKa = 3.92 SSSDD90 pKa = 4.1 IIEE93 pKa = 4.57 NDD95 pKa = 3.76 PLPAEE100 pKa = 4.56 PSDD103 pKa = 3.9 TNAEE107 pKa = 3.92 PSLPDD112 pKa = 3.39 TNGVCEE118 pKa = 4.35 VEE120 pKa = 4.25 DD121 pKa = 3.46 ALGFRR126 pKa = 11.84 EE127 pKa = 4.84 CLAQEE132 pKa = 3.73 KK133 pKa = 10.29 DD134 pKa = 3.1 IRR136 pKa = 11.84 LTLLSDD142 pKa = 3.68 VSVNAADD149 pKa = 4.87 LPNADD154 pKa = 4.49 TEE156 pKa = 4.36 FLSIDD161 pKa = 3.9 LNGHH165 pKa = 5.41 QLTIVIDD172 pKa = 3.76 EE173 pKa = 5.01 DD174 pKa = 5.19 SNFTPLRR181 pKa = 11.84 CSSFQLISEE190 pKa = 4.39 SEE192 pKa = 4.19 HH193 pKa = 8.7 AGMLSVKK200 pKa = 9.93 AASSMSAPFLAVSSYY215 pKa = 11.72 AFFAGSEE222 pKa = 4.07 AGIQIYY228 pKa = 9.26 SQSADD233 pKa = 3.14 KK234 pKa = 10.56 QAYY237 pKa = 7.97 TMIDD241 pKa = 4.08 LIGDD245 pKa = 3.87 AEE247 pKa = 4.2 MNAQNSRR254 pKa = 11.84 FMTNTGDD261 pKa = 3.56 VLTLITRR268 pKa = 11.84 EE269 pKa = 4.23 GNKK272 pKa = 9.94 LPQTAWIEE280 pKa = 3.87 FANIDD285 pKa = 3.44 QHH287 pKa = 9.42 AEE289 pKa = 3.82 FLNQQILYY297 pKa = 9.19 YY298 pKa = 8.95 YY299 pKa = 10.43 LPASPFLYY307 pKa = 10.67 AEE309 pKa = 3.91 SDD311 pKa = 3.37 KK312 pKa = 11.41 YY313 pKa = 10.27 QVQIEE318 pKa = 4.31 WGDD321 pKa = 3.36 LSYY324 pKa = 11.26 SYY326 pKa = 11.35 DD327 pKa = 3.68 GDD329 pKa = 3.66 RR330 pKa = 11.84 MSWQGNDD337 pKa = 3.04 NLNNHH342 pKa = 5.81 IRR344 pKa = 11.84 VNNYY348 pKa = 9.76 SLFPVMITHH357 pKa = 7.3 EE358 pKa = 4.3 LQLDD362 pKa = 3.7 DD363 pKa = 5.51 GNIKK367 pKa = 10.68 GDD369 pKa = 3.59 VYY371 pKa = 10.18 KK372 pKa = 10.26 TAGSKK377 pKa = 10.55 SDD379 pKa = 3.64 GSDD382 pKa = 3.06 EE383 pKa = 4.13 YY384 pKa = 11.36 DD385 pKa = 3.4 YY386 pKa = 12.05 DD387 pKa = 3.79 MCEE390 pKa = 3.4 LAAYY394 pKa = 9.69 EE395 pKa = 4.4 EE396 pKa = 4.37 EE397 pKa = 5.02 LIAAASNLPTQLDD410 pKa = 4.39 LYY412 pKa = 10.87 VVLSGNPDD420 pKa = 3.22 LDD422 pKa = 3.58 QYY424 pKa = 11.42 KK425 pKa = 9.44 HH426 pKa = 5.39 QKK428 pKa = 9.98 QIGSLSLTVTSMEE441 pKa = 5.09 NEE443 pKa = 3.97 PQNLNEE449 pKa = 4.09 PAEE452 pKa = 4.39 AFDD455 pKa = 3.67 LTIEE459 pKa = 4.19 KK460 pKa = 10.86 GEE462 pKa = 4.02 TLILSGEE469 pKa = 4.13 HH470 pKa = 5.85 EE471 pKa = 4.72 MNGNIYY477 pKa = 9.73 RR478 pKa = 11.84 VSGGGTLCFDD488 pKa = 3.45 QFQAVDD494 pKa = 3.73 ALDD497 pKa = 4.11 GDD499 pKa = 4.32 VLAEE503 pKa = 4.12 CDD505 pKa = 3.9 EE506 pKa = 4.78 DD507 pKa = 4.41 SSLIAGSHH515 pKa = 5.42 QDD517 pKa = 3.17 LNAYY521 pKa = 10.04 RR522 pKa = 11.84 FIDD525 pKa = 3.75 LLGEE529 pKa = 4.06 EE530 pKa = 4.62 TTAGTPAVKK539 pKa = 10.43 LIYY542 pKa = 9.98 RR543 pKa = 11.84 KK544 pKa = 10.3 EE545 pKa = 4.04 EE546 pKa = 4.1 ADD548 pKa = 3.7 VKK550 pKa = 11.51 ANDD553 pKa = 3.84 TNEE556 pKa = 4.26 TADD559 pKa = 3.95 EE560 pKa = 4.21 QPQQ563 pKa = 3.16
Molecular weight: 61.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.579
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.617
EMBOSS 3.681
Sillero 3.91
Patrickios 1.481
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A2V2FF10|A0A2V2FF10_9FIRM PTS sugar transporter subunit IIC OS=Dielma fastidiosa OX=1034346 GN=DES51_102182 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSKK9 pKa = 10.05 RR10 pKa = 11.84 KK11 pKa = 8.47 HH12 pKa = 5.31 QKK14 pKa = 6.82 THH16 pKa = 4.76 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.59 GRR39 pKa = 11.84 KK40 pKa = 8.44 VLSAA44 pKa = 4.05
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.31
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.047
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.016
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3467
0
3467
1067974
27
4918
308.0
34.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.617 ± 0.048
1.563 ± 0.019
5.807 ± 0.038
6.907 ± 0.04
4.294 ± 0.031
6.256 ± 0.052
1.968 ± 0.023
8.035 ± 0.042
6.727 ± 0.036
9.966 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.127 ± 0.023
4.925 ± 0.032
3.05 ± 0.02
3.623 ± 0.028
3.663 ± 0.032
5.928 ± 0.034
5.066 ± 0.041
6.409 ± 0.035
0.856 ± 0.015
4.212 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here