Prevotella brunnea

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8GDU6|A0A5C8GDU6_9BACT Uncharacterized protein OS=Prevotella brunnea OX=2508867 GN=ETF27_08565 PE=4 SV=1
MM1 pKa = 7.29NRR3 pKa = 11.84TTEE6 pKa = 5.08KK7 pKa = 10.34IPTWSLCYY15 pKa = 10.25LINGDD20 pKa = 3.82ATALTDD26 pKa = 3.99DD27 pKa = 5.37EE28 pKa = 4.53IRR30 pKa = 11.84MVDD33 pKa = 3.11EE34 pKa = 5.66WVSQWQVEE42 pKa = 4.53IVSPLMDD49 pKa = 4.41EE50 pKa = 5.32DD51 pKa = 5.27GSTHH55 pKa = 7.26PYY57 pKa = 9.29FSHH60 pKa = 6.54YY61 pKa = 10.23PLFGLPTEE69 pKa = 4.68VEE71 pKa = 4.21DD72 pKa = 6.23CEE74 pKa = 4.79ILYY77 pKa = 11.05KK78 pKa = 10.52NDD80 pKa = 3.18NLL82 pKa = 4.48

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8GBD4|A0A5C8GBD4_9BACT Site-specific integrase OS=Prevotella brunnea OX=2508867 GN=ETF27_09820 PE=4 SV=1
MM1 pKa = 7.35NGHH4 pKa = 6.47MPNCRR9 pKa = 11.84HH10 pKa = 4.6ITARR14 pKa = 11.84HH15 pKa = 4.46LTAIIAALLLLVMEE29 pKa = 5.69GCTSSRR35 pKa = 11.84FGEE38 pKa = 4.69GGHH41 pKa = 6.21TFRR44 pKa = 11.84QDD46 pKa = 2.83NPEE49 pKa = 3.38IDD51 pKa = 3.57RR52 pKa = 11.84RR53 pKa = 11.84FQIADD58 pKa = 3.38LGGQQLEE65 pKa = 3.98AATRR69 pKa = 11.84YY70 pKa = 9.97GLSKK74 pKa = 10.17PIRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84SGIWLRR85 pKa = 11.84AWRR88 pKa = 11.84LRR90 pKa = 11.84KK91 pKa = 9.49IKK93 pKa = 9.18TNRR96 pKa = 11.84YY97 pKa = 8.44YY98 pKa = 11.26LLDD101 pKa = 4.33PMPHH105 pKa = 6.2SNPYY109 pKa = 8.14LTSGGKK115 pKa = 8.42WVLKK119 pKa = 9.53TIAKK123 pKa = 9.94RR124 pKa = 11.84FQKK127 pKa = 10.44KK128 pKa = 8.5LRR130 pKa = 11.84KK131 pKa = 9.34AGYY134 pKa = 8.0RR135 pKa = 11.84EE136 pKa = 3.54HH137 pKa = 7.47RR138 pKa = 11.84IIVTSMLRR146 pKa = 11.84TRR148 pKa = 11.84EE149 pKa = 4.23DD150 pKa = 2.68IGGLKK155 pKa = 10.11KK156 pKa = 10.28INRR159 pKa = 11.84VAATNSSHH167 pKa = 6.78MYY169 pKa = 8.37GTTFDD174 pKa = 3.5VSFTRR179 pKa = 11.84FNRR182 pKa = 11.84ISMDD186 pKa = 2.8GMQPSNHH193 pKa = 6.01LMANLLGEE201 pKa = 4.65VICEE205 pKa = 3.78LRR207 pKa = 11.84KK208 pKa = 10.38EE209 pKa = 4.41GEE211 pKa = 3.77CWIIFEE217 pKa = 4.55RR218 pKa = 11.84SQHH221 pKa = 5.68CFHH224 pKa = 6.02VTARR228 pKa = 11.84RR229 pKa = 3.41

Molecular weight: 26.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2080

0

2080

716885

10

4198

344.7

38.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.981 ± 0.053

1.241 ± 0.022

5.53 ± 0.036

6.46 ± 0.055

4.584 ± 0.036

6.708 ± 0.052

2.06 ± 0.023

7.084 ± 0.053

7.172 ± 0.042

8.924 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.86 ± 0.026

5.325 ± 0.051

3.57 ± 0.027

3.49 ± 0.033

4.907 ± 0.045

5.899 ± 0.044

5.691 ± 0.04

6.219 ± 0.041

1.137 ± 0.021

4.156 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski