Pseudoalteromonas phage AL

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XVF8|A0A6G6XVF8_9CAUD Putative HNH endonuclease OS=Pseudoalteromonas phage AL OX=2712933 PE=4 SV=1
MM1 pKa = 7.34TPAEE5 pKa = 4.51YY6 pKa = 10.52LVLWDD11 pKa = 4.04YY12 pKa = 11.58KK13 pKa = 10.75YY14 pKa = 10.59ATAPEE19 pKa = 4.19YY20 pKa = 10.72EE21 pKa = 4.45EE22 pKa = 3.78QRR24 pKa = 11.84KK25 pKa = 9.47ASEE28 pKa = 4.26LDD30 pKa = 3.12QALIDD35 pKa = 3.89NWDD38 pKa = 3.67LLLL41 pKa = 5.35

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XVA7|A0A6G6XVA7_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage AL OX=2712933 PE=4 SV=1
MM1 pKa = 7.48NNLSDD6 pKa = 3.71MTDD9 pKa = 3.04LSFTQTKK16 pKa = 9.99RR17 pKa = 11.84AMTSTHH23 pKa = 6.87LSRR26 pKa = 11.84VSHH29 pKa = 6.32QYY31 pKa = 10.67RR32 pKa = 11.84PMWAIAAKK40 pKa = 10.46NNAKK44 pKa = 9.96PKK46 pKa = 9.72VVIDD50 pKa = 3.65RR51 pKa = 11.84VRR53 pKa = 11.84DD54 pKa = 3.88GKK56 pKa = 10.8RR57 pKa = 11.84SNPTVFMGCC66 pKa = 3.58

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10219

30

779

196.5

21.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.778 ± 0.635

0.959 ± 0.134

6.322 ± 0.375

6.86 ± 0.328

3.934 ± 0.187

7.124 ± 0.509

1.527 ± 0.182

6.635 ± 0.211

7.496 ± 0.531

8.494 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.201

5.421 ± 0.23

2.887 ± 0.254

3.719 ± 0.238

4.002 ± 0.299

6.39 ± 0.406

5.999 ± 0.312

6.038 ± 0.227

1.213 ± 0.133

3.582 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski