Notechis scutatus (mainland tiger snake)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J1W5P7|A0A6J1W5P7_9SAUR GTP-binding protein 1 OS=Notechis scutatus OX=8663 GN=GTPBP1 PE=4 SV=1
CC1 pKa = 7.44LFANPCFAGSKK12 pKa = 9.47CLNIAPGFRR21 pKa = 11.84CEE23 pKa = 4.09PCPLGYY29 pKa = 10.2KK30 pKa = 10.29GNLVTGVGADD40 pKa = 3.39YY41 pKa = 11.32AKK43 pKa = 10.6ASKK46 pKa = 10.12QICTDD51 pKa = 2.87VDD53 pKa = 3.5EE54 pKa = 5.98CNDD57 pKa = 3.93GNNGGCDD64 pKa = 3.43PNAICTNTVGSFKK77 pKa = 10.74CGPCKK82 pKa = 10.51SGFVEE87 pKa = 5.16KK88 pKa = 10.59EE89 pKa = 4.02PGSCTPQKK97 pKa = 10.57ACEE100 pKa = 4.33SPSHH104 pKa = 6.11NPCDD108 pKa = 3.86VNGYY112 pKa = 9.86CLFEE116 pKa = 4.89RR117 pKa = 11.84NGDD120 pKa = 3.75VSCSCNVGWAGNGNVCGRR138 pKa = 11.84DD139 pKa = 3.42TDD141 pKa = 3.37IDD143 pKa = 4.4GYY145 pKa = 10.04PDD147 pKa = 3.67EE148 pKa = 5.4PLPCIDD154 pKa = 4.3NNKK157 pKa = 9.47HH158 pKa = 5.93CAQDD162 pKa = 3.59NCRR165 pKa = 11.84LTPNSGQEE173 pKa = 3.95DD174 pKa = 4.26ADD176 pKa = 3.62NDD178 pKa = 5.11GIGDD182 pKa = 3.82QCDD185 pKa = 3.57DD186 pKa = 4.52DD187 pKa = 6.87ADD189 pKa = 4.09GDD191 pKa = 4.37GIKK194 pKa = 10.51NVEE197 pKa = 4.41DD198 pKa = 3.59NCRR201 pKa = 11.84LLPNKK206 pKa = 9.71DD207 pKa = 3.66QQNSDD212 pKa = 3.62PDD214 pKa = 4.13SFGDD218 pKa = 4.57ACDD221 pKa = 3.62NCPNVPNNDD230 pKa = 4.18QKK232 pKa = 11.26DD233 pKa = 3.45TDD235 pKa = 3.74QNGEE239 pKa = 3.91GDD241 pKa = 3.73ACDD244 pKa = 3.98NDD246 pKa = 3.39IDD248 pKa = 5.49GDD250 pKa = 4.41GIPNGLDD257 pKa = 3.15NCPKK261 pKa = 10.23VPNPLQTDD269 pKa = 3.4RR270 pKa = 11.84DD271 pKa = 3.67EE272 pKa = 6.42DD273 pKa = 4.4GVGDD277 pKa = 4.27ACDD280 pKa = 3.57SCPEE284 pKa = 4.33LSNPTQTDD292 pKa = 2.88VDD294 pKa = 4.03SDD296 pKa = 4.44LVGDD300 pKa = 4.12ACDD303 pKa = 3.65TNEE306 pKa = 4.67DD307 pKa = 3.81RR308 pKa = 11.84DD309 pKa = 4.1GDD311 pKa = 3.98GHH313 pKa = 7.3QDD315 pKa = 3.14TKK317 pKa = 11.44DD318 pKa = 3.42NCVEE322 pKa = 4.27IPNSSQLDD330 pKa = 3.68SDD332 pKa = 4.3NDD334 pKa = 4.23GQGDD338 pKa = 4.0DD339 pKa = 5.38CDD341 pKa = 5.28NDD343 pKa = 4.19DD344 pKa = 5.42DD345 pKa = 5.52NDD347 pKa = 5.14GIPDD351 pKa = 4.17YY352 pKa = 10.95LPPGPDD358 pKa = 3.02NCRR361 pKa = 11.84LIANPNQKK369 pKa = 10.35DD370 pKa = 3.34VDD372 pKa = 4.1GNGVGDD378 pKa = 3.67ACEE381 pKa = 3.98EE382 pKa = 4.22DD383 pKa = 3.85FDD385 pKa = 5.35NDD387 pKa = 3.74TVADD391 pKa = 5.0PIDD394 pKa = 3.65VCPEE398 pKa = 3.74SSEE401 pKa = 4.04VTLTDD406 pKa = 3.73FRR408 pKa = 11.84AYY410 pKa = 8.36QTVILDD416 pKa = 3.91PEE418 pKa = 5.2GDD420 pKa = 3.69AQIDD424 pKa = 4.13PNWVVLNQGMEE435 pKa = 4.11IVQTMNSDD443 pKa = 3.19PGLAVGYY450 pKa = 7.31TAFNGVDD457 pKa = 3.99FEE459 pKa = 4.97GTFHH463 pKa = 6.68VNTMTDD469 pKa = 2.95DD470 pKa = 4.36DD471 pKa = 4.35YY472 pKa = 12.02AGFIFAYY479 pKa = 9.53QDD481 pKa = 3.19SASFYY486 pKa = 10.36VVMWKK491 pKa = 7.78QTEE494 pKa = 3.86QTYY497 pKa = 8.13WQATPFRR504 pKa = 11.84AVAEE508 pKa = 4.33SSLQLKK514 pKa = 9.19AVKK517 pKa = 10.44SEE519 pKa = 4.18TGPGEE524 pKa = 4.19YY525 pKa = 10.12LRR527 pKa = 11.84NALWHH532 pKa = 6.17TGHH535 pKa = 6.64TPGHH539 pKa = 6.2VKK541 pKa = 10.49LLWKK545 pKa = 10.39DD546 pKa = 3.35PRR548 pKa = 11.84NVGWKK553 pKa = 10.34DD554 pKa = 2.96KK555 pKa = 10.8TSYY558 pKa = 10.16RR559 pKa = 11.84WRR561 pKa = 11.84LLHH564 pKa = 6.59RR565 pKa = 11.84PQVGYY570 pKa = 10.39IRR572 pKa = 4.61

Molecular weight: 61.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J1W3W9|A0A6J1W3W9_9SAUR serpin B3-like OS=Notechis scutatus OX=8663 GN=LOC113431791 PE=4 SV=1
MM1 pKa = 7.72LKK3 pKa = 10.06NKK5 pKa = 9.86QPIPVNIRR13 pKa = 11.84ATMQQQQLASARR25 pKa = 11.84NRR27 pKa = 11.84RR28 pKa = 11.84LAQQMEE34 pKa = 4.32NRR36 pKa = 11.84PSVQAALKK44 pKa = 10.37LKK46 pKa = 10.39QKK48 pKa = 9.42SLKK51 pKa = 9.72QRR53 pKa = 11.84LGKK56 pKa = 10.77SNIQARR62 pKa = 11.84LGRR65 pKa = 11.84PALARR70 pKa = 11.84GAFGGRR76 pKa = 11.84GLPMGQRR83 pKa = 11.84GLARR87 pKa = 11.84GALARR92 pKa = 11.84GGRR95 pKa = 11.84GARR98 pKa = 11.84GLLRR102 pKa = 11.84GGIPLRR108 pKa = 11.84GHH110 pKa = 5.58SLLRR114 pKa = 11.84GGRR117 pKa = 11.84GLGPRR122 pKa = 11.84MGLRR126 pKa = 11.84RR127 pKa = 11.84GGMRR131 pKa = 11.84GRR133 pKa = 11.84AGPGRR138 pKa = 11.84GGLGRR143 pKa = 11.84GAMGRR148 pKa = 11.84GGLGGRR154 pKa = 11.84GEE156 pKa = 4.31WNGPEE161 pKa = 4.2GQQ163 pKa = 3.58

Molecular weight: 17.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19407

7322

26729

17436458

31

33049

652.3

73.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.345 ± 0.013

2.124 ± 0.014

4.887 ± 0.01

7.526 ± 0.028

3.646 ± 0.016

5.96 ± 0.017

2.512 ± 0.015

5.101 ± 0.02

6.631 ± 0.034

9.321 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.014

4.123 ± 0.016

5.833 ± 0.026

4.684 ± 0.033

5.167 ± 0.012

8.417 ± 0.026

5.537 ± 0.026

6.018 ± 0.038

1.154 ± 0.005

2.807 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski