Domibacillus epiphyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Domibacillus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2A6U5|A0A1V2A6U5_9BACI HD domain-containing protein OS=Domibacillus epiphyticus OX=1714355 GN=BTO28_10895 PE=4 SV=1
MM1 pKa = 7.16YY2 pKa = 10.42CSTCGTNLGRR12 pKa = 11.84SSRR15 pKa = 11.84YY16 pKa = 9.83CPGCGSKK23 pKa = 10.1RR24 pKa = 11.84GRR26 pKa = 11.84RR27 pKa = 11.84FSYY30 pKa = 10.46VKK32 pKa = 10.53LIAFLCIASVLAFAGTIYY50 pKa = 11.02YY51 pKa = 10.57LLSNDD56 pKa = 4.39EE57 pKa = 4.27KK58 pKa = 10.51TQTASQTAIKK68 pKa = 9.55TPTEE72 pKa = 3.77QSADD76 pKa = 3.39TRR78 pKa = 11.84KK79 pKa = 7.58TVAPPEE85 pKa = 4.11EE86 pKa = 4.84LSAEE90 pKa = 4.23DD91 pKa = 3.66QQSLTEE97 pKa = 4.72IIAAAQQNVYY107 pKa = 10.14TIFTDD112 pKa = 3.34SGQGSGFLINDD123 pKa = 3.87RR124 pKa = 11.84GDD126 pKa = 3.43VATNAHH132 pKa = 5.11VVEE135 pKa = 4.92GSVSVIVKK143 pKa = 10.44DD144 pKa = 3.34IDD146 pKa = 3.91GVEE149 pKa = 4.19HH150 pKa = 7.01EE151 pKa = 4.86GTVIGYY157 pKa = 8.66SNKK160 pKa = 9.36TDD162 pKa = 3.11VALIRR167 pKa = 11.84VPDD170 pKa = 3.97FADD173 pKa = 3.54YY174 pKa = 11.35SPLNLEE180 pKa = 4.4TNKK183 pKa = 10.1KK184 pKa = 9.29AQVGDD189 pKa = 3.67EE190 pKa = 4.4VIALGSPLALEE201 pKa = 4.12NTATFGYY208 pKa = 8.06ITGVDD213 pKa = 2.9RR214 pKa = 11.84SFIIEE219 pKa = 3.86PHH221 pKa = 6.47SYY223 pKa = 11.41DD224 pKa = 4.55NIYY227 pKa = 8.18QTSAAIAPGSSGGPLVSKK245 pKa = 9.57NTGRR249 pKa = 11.84VIAINSAKK257 pKa = 9.69LTGEE261 pKa = 3.94DD262 pKa = 4.08AIGFSIPYY270 pKa = 9.77KK271 pKa = 10.8DD272 pKa = 3.97VDD274 pKa = 4.45DD275 pKa = 4.44MLKK278 pKa = 9.74KK279 pKa = 10.04WSRR282 pKa = 11.84SPLSEE287 pKa = 4.38EE288 pKa = 3.93EE289 pKa = 4.35VNDD292 pKa = 4.29LFYY295 pKa = 11.09TEE297 pKa = 5.33DD298 pKa = 3.37GSLFFEE304 pKa = 4.96EE305 pKa = 5.08YY306 pKa = 9.54WNEE309 pKa = 3.64DD310 pKa = 3.15AYY312 pKa = 11.33FDD314 pKa = 4.05GGEE317 pKa = 4.11YY318 pKa = 10.36SDD320 pKa = 4.22DD321 pKa = 3.61ASYY324 pKa = 11.73DD325 pKa = 3.71FYY327 pKa = 11.25DD328 pKa = 5.19IPADD332 pKa = 2.78WWYY335 pKa = 10.7EE336 pKa = 3.49EE337 pKa = 4.05DD338 pKa = 3.96TYY340 pKa = 11.43EE341 pKa = 4.96DD342 pKa = 4.32EE343 pKa = 4.96YY344 pKa = 11.11IEE346 pKa = 4.17EE347 pKa = 4.32VPEE350 pKa = 3.92EE351 pKa = 4.69EE352 pKa = 4.84IPSTEE357 pKa = 3.75PDD359 pKa = 3.55DD360 pKa = 4.69YY361 pKa = 11.63EE362 pKa = 4.3EE363 pKa = 4.12EE364 pKa = 4.46TIEE367 pKa = 4.04EE368 pKa = 4.29EE369 pKa = 4.69PIEE372 pKa = 4.25EE373 pKa = 4.21LPLIEE378 pKa = 5.04EE379 pKa = 4.48EE380 pKa = 4.58PMPDD384 pKa = 3.63TAPDD388 pKa = 4.23PYY390 pKa = 11.26LEE392 pKa = 5.53DD393 pKa = 3.51INGDD397 pKa = 3.61GLIDD401 pKa = 3.91VNDD404 pKa = 3.53VTEE407 pKa = 5.32DD408 pKa = 3.18INMDD412 pKa = 3.69GVIDD416 pKa = 4.31EE417 pKa = 5.28LDD419 pKa = 3.74LDD421 pKa = 4.65EE422 pKa = 6.2LLMMQQ427 pKa = 4.84

Molecular weight: 47.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2A6M7|A0A1V2A6M7_9BACI MFS transporter OS=Domibacillus epiphyticus OX=1714355 GN=BTO28_12375 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.74VLSAA44 pKa = 4.05

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3171

0

3171

908978

27

1529

286.7

32.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.866 ± 0.047

0.719 ± 0.014

5.169 ± 0.031

7.622 ± 0.052

4.503 ± 0.036

7.144 ± 0.039

2.117 ± 0.021

7.518 ± 0.044

6.582 ± 0.042

9.418 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.021

4.05 ± 0.031

3.769 ± 0.027

3.451 ± 0.027

4.319 ± 0.032

6.024 ± 0.031

5.402 ± 0.03

7.143 ± 0.032

0.976 ± 0.016

3.273 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski