Amino acid dipepetide frequency for Domibacillus epiphyticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.713AlaAla: 8.713 ± 0.141
0.6AlaCys: 0.6 ± 0.026
4.107AlaAsp: 4.107 ± 0.072
5.467AlaGlu: 5.467 ± 0.097
3.602AlaPhe: 3.602 ± 0.071
6.818AlaGly: 6.818 ± 0.09
1.44AlaHis: 1.44 ± 0.043
5.892AlaIle: 5.892 ± 0.087
4.712AlaLys: 4.712 ± 0.073
7.582AlaLeu: 7.582 ± 0.099
2.217AlaMet: 2.217 ± 0.054
2.66AlaAsn: 2.66 ± 0.051
2.419AlaPro: 2.419 ± 0.058
2.183AlaGln: 2.183 ± 0.057
3.173AlaArg: 3.173 ± 0.061
4.568AlaSer: 4.568 ± 0.068
3.004AlaThr: 3.004 ± 0.068
6.642AlaVal: 6.642 ± 0.093
0.586AlaTrp: 0.586 ± 0.028
2.281AlaTyr: 2.281 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.502CysAla: 0.502 ± 0.023
0.092CysCys: 0.092 ± 0.011
0.405CysAsp: 0.405 ± 0.022
0.436CysGlu: 0.436 ± 0.023
0.307CysPhe: 0.307 ± 0.018
0.745CysGly: 0.745 ± 0.032
0.199CysHis: 0.199 ± 0.014
0.512CysIle: 0.512 ± 0.026
0.281CysLys: 0.281 ± 0.017
0.67CysLeu: 0.67 ± 0.024
0.179CysMet: 0.179 ± 0.015
0.246CysAsn: 0.246 ± 0.015
0.394CysPro: 0.394 ± 0.022
0.212CysGln: 0.212 ± 0.015
0.369CysArg: 0.369 ± 0.021
0.494CysSer: 0.494 ± 0.024
0.428CysThr: 0.428 ± 0.025
0.458CysVal: 0.458 ± 0.024
0.057CysTrp: 0.057 ± 0.008
0.207CysTyr: 0.207 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.637AspAla: 3.637 ± 0.066
0.392AspCys: 0.392 ± 0.022
2.635AspAsp: 2.635 ± 0.071
4.575AspGlu: 4.575 ± 0.073
2.266AspPhe: 2.266 ± 0.054
3.666AspGly: 3.666 ± 0.054
1.199AspHis: 1.199 ± 0.036
4.123AspIle: 4.123 ± 0.074
2.823AspLys: 2.823 ± 0.06
4.797AspLeu: 4.797 ± 0.064
1.419AspMet: 1.419 ± 0.039
1.636AspAsn: 1.636 ± 0.044
2.113AspPro: 2.113 ± 0.052
1.954AspGln: 1.954 ± 0.048
2.517AspArg: 2.517 ± 0.06
2.674AspSer: 2.674 ± 0.053
2.437AspThr: 2.437 ± 0.058
4.197AspVal: 4.197 ± 0.075
0.585AspTrp: 0.585 ± 0.026
2.043AspTyr: 2.043 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
6.017GluAla: 6.017 ± 0.09
0.365GluCys: 0.365 ± 0.02
3.596GluAsp: 3.596 ± 0.064
7.263GluGlu: 7.263 ± 0.109
2.536GluPhe: 2.536 ± 0.045
4.413GluGly: 4.413 ± 0.083
1.519GluHis: 1.519 ± 0.038
5.198GluIle: 5.198 ± 0.078
6.767GluLys: 6.767 ± 0.106
7.054GluLeu: 7.054 ± 0.097
2.509GluMet: 2.509 ± 0.049
3.443GluAsn: 3.443 ± 0.066
2.122GluPro: 2.122 ± 0.049
3.514GluGln: 3.514 ± 0.076
3.893GluArg: 3.893 ± 0.076
3.901GluSer: 3.901 ± 0.07
4.317GluThr: 4.317 ± 0.08
4.734GluVal: 4.734 ± 0.085
0.879GluTrp: 0.879 ± 0.032
2.18GluTyr: 2.18 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.217PheAla: 3.217 ± 0.065
0.364PheCys: 0.364 ± 0.021
2.414PheAsp: 2.414 ± 0.051
2.881PheGlu: 2.881 ± 0.054
2.55PhePhe: 2.55 ± 0.071
3.376PheGly: 3.376 ± 0.073
1.068PheHis: 1.068 ± 0.033
3.727PheIle: 3.727 ± 0.09
2.128PheLys: 2.128 ± 0.052
4.601PheLeu: 4.601 ± 0.091
1.254PheMet: 1.254 ± 0.039
1.663PheAsn: 1.663 ± 0.045
1.65PhePro: 1.65 ± 0.046
1.527PheGln: 1.527 ± 0.041
1.621PheArg: 1.621 ± 0.042
3.122PheSer: 3.122 ± 0.062
2.578PheThr: 2.578 ± 0.048
3.16PheVal: 3.16 ± 0.064
0.461PheTrp: 0.461 ± 0.021
1.666PheTyr: 1.666 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
5.551GlyAla: 5.551 ± 0.087
0.63GlyCys: 0.63 ± 0.026
3.383GlyAsp: 3.383 ± 0.067
4.777GlyGlu: 4.777 ± 0.077
3.448GlyPhe: 3.448 ± 0.062
5.162GlyGly: 5.162 ± 0.094
1.562GlyHis: 1.562 ± 0.042
5.908GlyIle: 5.908 ± 0.089
4.945GlyLys: 4.945 ± 0.076
6.742GlyLeu: 6.742 ± 0.097
2.19GlyMet: 2.19 ± 0.053
2.566GlyAsn: 2.566 ± 0.051
1.946GlyPro: 1.946 ± 0.058
2.239GlyGln: 2.239 ± 0.058
3.117GlyArg: 3.117 ± 0.067
4.269GlySer: 4.269 ± 0.076
4.236GlyThr: 4.236 ± 0.07
5.226GlyVal: 5.226 ± 0.08
0.815GlyTrp: 0.815 ± 0.031
2.731GlyTyr: 2.731 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.408HisAla: 1.408 ± 0.04
0.208HisCys: 0.208 ± 0.015
1.12HisAsp: 1.12 ± 0.039
1.513HisGlu: 1.513 ± 0.04
1.111HisPhe: 1.111 ± 0.04
1.524HisGly: 1.524 ± 0.042
0.671HisHis: 0.671 ± 0.029
1.601HisIle: 1.601 ± 0.045
0.996HisLys: 0.996 ± 0.031
2.026HisLeu: 2.026 ± 0.047
0.532HisMet: 0.532 ± 0.023
0.755HisAsn: 0.755 ± 0.03
1.222HisPro: 1.222 ± 0.04
0.675HisGln: 0.675 ± 0.027
0.879HisArg: 0.879 ± 0.03
1.314HisSer: 1.314 ± 0.038
1.063HisThr: 1.063 ± 0.033
1.565HisVal: 1.565 ± 0.044
0.195HisTrp: 0.195 ± 0.014
0.795HisTyr: 0.795 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.452IleAla: 6.452 ± 0.1
0.606IleCys: 0.606 ± 0.027
4.374IleAsp: 4.374 ± 0.068
5.771IleGlu: 5.771 ± 0.083
3.033IlePhe: 3.033 ± 0.077
6.056IleGly: 6.056 ± 0.091
1.71IleHis: 1.71 ± 0.046
5.44IleIle: 5.44 ± 0.107
3.843IleLys: 3.843 ± 0.074
6.486IleLeu: 6.486 ± 0.107
1.854IleMet: 1.854 ± 0.046
2.92IleAsn: 2.92 ± 0.059
3.266IlePro: 3.266 ± 0.065
2.833IleGln: 2.833 ± 0.059
3.35IleArg: 3.35 ± 0.075
4.776IleSer: 4.776 ± 0.074
4.162IleThr: 4.162 ± 0.058
5.464IleVal: 5.464 ± 0.089
0.624IleTrp: 0.624 ± 0.027
2.155IleTyr: 2.155 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.784LysAla: 4.784 ± 0.075
0.316LysCys: 0.316 ± 0.019
3.359LysAsp: 3.359 ± 0.06
6.783LysGlu: 6.783 ± 0.084
1.799LysPhe: 1.799 ± 0.052
4.324LysGly: 4.324 ± 0.069
1.201LysHis: 1.201 ± 0.037
4.164LysIle: 4.164 ± 0.07
6.08LysLys: 6.08 ± 0.086
5.208LysLeu: 5.208 ± 0.074
2.437LysMet: 2.437 ± 0.052
2.985LysAsn: 2.985 ± 0.062
2.266LysPro: 2.266 ± 0.055
2.715LysGln: 2.715 ± 0.058
3.319LysArg: 3.319 ± 0.075
3.331LysSer: 3.331 ± 0.055
3.935LysThr: 3.935 ± 0.072
4.144LysVal: 4.144 ± 0.066
0.796LysTrp: 0.796 ± 0.03
1.876LysTyr: 1.876 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.489LeuAla: 7.489 ± 0.104
0.623LeuCys: 0.623 ± 0.026
4.729LeuAsp: 4.729 ± 0.067
6.21LeuGlu: 6.21 ± 0.087
4.908LeuPhe: 4.908 ± 0.101
6.0LeuGly: 6.0 ± 0.103
2.0LeuHis: 2.0 ± 0.043
6.737LeuIle: 6.737 ± 0.11
6.332LeuLys: 6.332 ± 0.091
9.898LeuLeu: 9.898 ± 0.146
2.621LeuMet: 2.621 ± 0.055
4.011LeuAsn: 4.011 ± 0.061
3.934LeuPro: 3.934 ± 0.075
3.197LeuGln: 3.197 ± 0.058
3.575LeuArg: 3.575 ± 0.074
6.808LeuSer: 6.808 ± 0.086
5.599LeuThr: 5.599 ± 0.071
5.699LeuVal: 5.699 ± 0.085
0.761LeuTrp: 0.761 ± 0.032
3.045LeuTyr: 3.045 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.361MetAla: 2.361 ± 0.052
0.179MetCys: 0.179 ± 0.015
1.564MetAsp: 1.564 ± 0.036
2.141MetGlu: 2.141 ± 0.051
1.102MetPhe: 1.102 ± 0.035
1.786MetGly: 1.786 ± 0.047
0.414MetHis: 0.414 ± 0.02
2.307MetIle: 2.307 ± 0.054
2.636MetLys: 2.636 ± 0.05
2.5MetLeu: 2.5 ± 0.058
1.022MetMet: 1.022 ± 0.038
1.678MetAsn: 1.678 ± 0.041
1.169MetPro: 1.169 ± 0.044
0.933MetGln: 0.933 ± 0.029
1.212MetArg: 1.212 ± 0.037
1.662MetSer: 1.662 ± 0.045
2.075MetThr: 2.075 ± 0.045
1.685MetVal: 1.685 ± 0.042
0.172MetTrp: 0.172 ± 0.015
0.755MetTyr: 0.755 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.838AsnAla: 2.838 ± 0.067
0.315AsnCys: 0.315 ± 0.019
2.1AsnAsp: 2.1 ± 0.055
3.622AsnGlu: 3.622 ± 0.058
1.319AsnPhe: 1.319 ± 0.042
3.456AsnGly: 3.456 ± 0.066
0.896AsnHis: 0.896 ± 0.033
3.055AsnIle: 3.055 ± 0.057
2.478AsnLys: 2.478 ± 0.064
3.221AsnLeu: 3.221 ± 0.065
1.229AsnMet: 1.229 ± 0.038
1.655AsnAsn: 1.655 ± 0.051
2.017AsnPro: 2.017 ± 0.055
1.453AsnGln: 1.453 ± 0.045
1.933AsnArg: 1.933 ± 0.05
2.053AsnSer: 2.053 ± 0.049
2.022AsnThr: 2.022 ± 0.048
3.145AsnVal: 3.145 ± 0.064
0.475AsnTrp: 0.475 ± 0.022
1.216AsnTyr: 1.216 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
3.047ProAla: 3.047 ± 0.066
0.239ProCys: 0.239 ± 0.019
2.525ProAsp: 2.525 ± 0.054
3.274ProGlu: 3.274 ± 0.067
2.149ProPhe: 2.149 ± 0.062
2.725ProGly: 2.725 ± 0.064
0.824ProHis: 0.824 ± 0.031
2.573ProIle: 2.573 ± 0.047
1.953ProLys: 1.953 ± 0.046
3.625ProLeu: 3.625 ± 0.067
0.814ProMet: 0.814 ± 0.029
1.475ProAsn: 1.475 ± 0.046
1.166ProPro: 1.166 ± 0.035
1.019ProGln: 1.019 ± 0.039
1.167ProArg: 1.167 ± 0.038
2.385ProSer: 2.385 ± 0.055
1.564ProThr: 1.564 ± 0.043
3.395ProVal: 3.395 ± 0.061
0.356ProTrp: 0.356 ± 0.021
1.414ProTyr: 1.414 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
2.626GlnAla: 2.626 ± 0.059
0.227GlnCys: 0.227 ± 0.017
1.481GlnAsp: 1.481 ± 0.039
2.663GlnGlu: 2.663 ± 0.061
1.571GlnPhe: 1.571 ± 0.044
1.88GlnGly: 1.88 ± 0.058
0.703GlnHis: 0.703 ± 0.027
2.473GlnIle: 2.473 ± 0.054
2.643GlnLys: 2.643 ± 0.056
3.482GlnLeu: 3.482 ± 0.064
1.18GlnMet: 1.18 ± 0.036
1.558GlnAsn: 1.558 ± 0.046
1.242GlnPro: 1.242 ± 0.04
1.518GlnGln: 1.518 ± 0.047
1.377GlnArg: 1.377 ± 0.043
2.208GlnSer: 2.208 ± 0.057
1.966GlnThr: 1.966 ± 0.046
2.095GlnVal: 2.095 ± 0.054
0.355GlnTrp: 0.355 ± 0.02
1.263GlnTyr: 1.263 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.903ArgAla: 2.903 ± 0.053
0.27ArgCys: 0.27 ± 0.019
2.311ArgAsp: 2.311 ± 0.05
3.325ArgGlu: 3.325 ± 0.067
2.041ArgPhe: 2.041 ± 0.054
2.566ArgGly: 2.566 ± 0.056
1.014ArgHis: 1.014 ± 0.038
3.172ArgIle: 3.172 ± 0.061
3.073ArgLys: 3.073 ± 0.058
4.339ArgLeu: 4.339 ± 0.078
1.437ArgMet: 1.437 ± 0.036
1.844ArgAsn: 1.844 ± 0.04
1.59ArgPro: 1.59 ± 0.044
1.552ArgGln: 1.552 ± 0.041
2.14ArgArg: 2.14 ± 0.051
2.514ArgSer: 2.514 ± 0.058
2.287ArgThr: 2.287 ± 0.049
2.865ArgVal: 2.865 ± 0.06
0.427ArgTrp: 0.427 ± 0.022
1.523ArgTyr: 1.523 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
4.429SerAla: 4.429 ± 0.072
0.427SerCys: 0.427 ± 0.025
2.878SerAsp: 2.878 ± 0.062
3.922SerGlu: 3.922 ± 0.065
3.335SerPhe: 3.335 ± 0.064
4.881SerGly: 4.881 ± 0.071
1.23SerHis: 1.23 ± 0.039
5.141SerIle: 5.141 ± 0.068
3.509SerLys: 3.509 ± 0.064
5.932SerLeu: 5.932 ± 0.091
1.772SerMet: 1.772 ± 0.048
2.19SerAsn: 2.19 ± 0.052
2.242SerPro: 2.242 ± 0.045
1.668SerGln: 1.668 ± 0.048
2.508SerArg: 2.508 ± 0.055
4.031SerSer: 4.031 ± 0.076
2.979SerThr: 2.979 ± 0.061
4.529SerVal: 4.529 ± 0.072
0.586SerTrp: 0.586 ± 0.028
2.047SerTyr: 2.047 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
4.918ThrAla: 4.918 ± 0.084
0.367ThrCys: 0.367 ± 0.02
2.843ThrAsp: 2.843 ± 0.059
3.769ThrGlu: 3.769 ± 0.062
2.621ThrPhe: 2.621 ± 0.051
4.799ThrGly: 4.799 ± 0.067
1.004ThrHis: 1.004 ± 0.037
4.32ThrIle: 4.32 ± 0.071
3.079ThrLys: 3.079 ± 0.057
4.851ThrLeu: 4.851 ± 0.062
1.383ThrMet: 1.383 ± 0.038
2.177ThrAsn: 2.177 ± 0.051
2.289ThrPro: 2.289 ± 0.046
1.315ThrGln: 1.315 ± 0.036
1.962ThrArg: 1.962 ± 0.044
2.988ThrSer: 2.988 ± 0.06
2.589ThrThr: 2.589 ± 0.055
4.558ThrVal: 4.558 ± 0.065
0.466ThrTrp: 0.466 ± 0.022
1.718ThrTyr: 1.718 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
4.901ValAla: 4.901 ± 0.091
0.608ValCys: 0.608 ± 0.029
3.542ValAsp: 3.542 ± 0.068
4.765ValGlu: 4.765 ± 0.086
3.2ValPhe: 3.2 ± 0.053
4.172ValGly: 4.172 ± 0.068
1.559ValHis: 1.559 ± 0.04
5.779ValIle: 5.779 ± 0.081
4.955ValLys: 4.955 ± 0.086
7.107ValLeu: 7.107 ± 0.098
2.088ValMet: 2.088 ± 0.048
3.2ValAsn: 3.2 ± 0.067
2.97ValPro: 2.97 ± 0.055
2.577ValGln: 2.577 ± 0.052
3.002ValArg: 3.002 ± 0.063
4.582ValSer: 4.582 ± 0.076
4.402ValThr: 4.402 ± 0.075
5.047ValVal: 5.047 ± 0.087
0.656ValTrp: 0.656 ± 0.029
2.321ValTyr: 2.321 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.636TrpAla: 0.636 ± 0.028
0.098TrpCys: 0.098 ± 0.01
0.457TrpAsp: 0.457 ± 0.024
0.592TrpGlu: 0.592 ± 0.031
0.505TrpPhe: 0.505 ± 0.026
0.627TrpGly: 0.627 ± 0.026
0.218TrpHis: 0.218 ± 0.016
0.78TrpIle: 0.78 ± 0.029
0.67TrpLys: 0.67 ± 0.026
1.087TrpLeu: 1.087 ± 0.038
0.367TrpMet: 0.367 ± 0.022
0.448TrpAsn: 0.448 ± 0.021
0.304TrpPro: 0.304 ± 0.018
0.333TrpGln: 0.333 ± 0.016
0.439TrpArg: 0.439 ± 0.024
0.589TrpSer: 0.589 ± 0.024
0.579TrpThr: 0.579 ± 0.027
0.63TrpVal: 0.63 ± 0.03
0.121TrpTrp: 0.121 ± 0.013
0.284TrpTyr: 0.284 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.234TyrAla: 2.234 ± 0.058
0.266TyrCys: 0.266 ± 0.02
1.866TyrAsp: 1.866 ± 0.046
2.473TyrGlu: 2.473 ± 0.058
1.626TyrPhe: 1.626 ± 0.044
2.468TyrGly: 2.468 ± 0.051
0.741TyrHis: 0.741 ± 0.031
2.273TyrIle: 2.273 ± 0.055
1.922TyrLys: 1.922 ± 0.044
2.97TyrLeu: 2.97 ± 0.062
0.846TyrMet: 0.846 ± 0.034
1.386TyrAsn: 1.386 ± 0.037
1.366TyrPro: 1.366 ± 0.041
1.095TyrGln: 1.095 ± 0.037
1.637TyrArg: 1.637 ± 0.044
1.97TyrSer: 1.97 ± 0.049
1.795TyrThr: 1.795 ± 0.045
2.196TyrVal: 2.196 ± 0.056
0.387TyrTrp: 0.387 ± 0.019
1.207TyrTyr: 1.207 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3171 proteins (908978 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski