Maverick-related virus strain Spezl
Average proteome isoelectric point is 7.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F1DAT9|F1DAT9_9VIRU Putative major capsid protein OS=Maverick-related virus strain Spezl OX=1932931 GN=MV18 PE=4 SV=1
MM1 pKa = 7.22 KK2 pKa = 10.29 QYY4 pKa = 10.37 IWLNEE9 pKa = 4.17 TIKK12 pKa = 11.0 SNKK15 pKa = 8.48 QLAGPRR21 pKa = 11.84 GSYY24 pKa = 9.41 KK25 pKa = 10.44 RR26 pKa = 11.84 PVSVDD31 pKa = 2.56 IFRR34 pKa = 11.84 SSTILDD40 pKa = 3.48 PDD42 pKa = 3.6 KK43 pKa = 11.34 NYY45 pKa = 11.07 LLIVEE50 pKa = 4.93 EE51 pKa = 4.12 FHH53 pKa = 6.26 LHH55 pKa = 7.51 KK56 pKa = 10.38 IRR58 pKa = 11.84 LPLFKK63 pKa = 10.05 PAGHH67 pKa = 7.61 DD68 pKa = 3.54 YY69 pKa = 10.85 QVGIFNRR76 pKa = 11.84 STDD79 pKa = 3.2 EE80 pKa = 3.72 IMGVRR85 pKa = 11.84 EE86 pKa = 3.83 VDD88 pKa = 3.93 FSTFVDD94 pKa = 3.67 EE95 pKa = 5.34 DD96 pKa = 4.67 GYY98 pKa = 10.96 MYY100 pKa = 10.59 DD101 pKa = 3.66 YY102 pKa = 11.45 VDD104 pKa = 3.52 VGTAINEE111 pKa = 4.27 TLAGLCDD118 pKa = 4.68 GIIGEE123 pKa = 4.22 EE124 pKa = 4.39 DD125 pKa = 3.14 IPVFSFNKK133 pKa = 9.24 HH134 pKa = 4.26 SKK136 pKa = 9.93 KK137 pKa = 10.74 FEE139 pKa = 3.67 ITTTEE144 pKa = 3.8 NFRR147 pKa = 11.84 NGHH150 pKa = 6.69 FIMFNDD156 pKa = 4.46 DD157 pKa = 2.97 MRR159 pKa = 11.84 VDD161 pKa = 4.44 FNSFEE166 pKa = 4.06 FDD168 pKa = 5.44 DD169 pKa = 3.98 IDD171 pKa = 3.7 EE172 pKa = 4.88 EE173 pKa = 4.63 YY174 pKa = 11.38 SLVILNEE181 pKa = 4.08 DD182 pKa = 4.28 VEE184 pKa = 4.61 TQDD187 pKa = 4.66 ASTLEE192 pKa = 3.95 FLTPISHH199 pKa = 7.26 IVIEE203 pKa = 4.67 SNDD206 pKa = 3.55 LPVSYY211 pKa = 10.25 EE212 pKa = 4.01 LLPSISKK219 pKa = 8.38 NTTISDD225 pKa = 3.51 NTGVFLTNYY234 pKa = 10.32 KK235 pKa = 10.24 YY236 pKa = 10.67 LQQNNQDD243 pKa = 3.57 YY244 pKa = 11.1 NSILFRR250 pKa = 11.84 VEE252 pKa = 3.53 NSSNKK257 pKa = 8.64 YY258 pKa = 10.56 HH259 pKa = 7.44 NILQTNFNRR268 pKa = 11.84 FNLSFTIYY276 pKa = 10.58 DD277 pKa = 3.73 YY278 pKa = 11.83 DD279 pKa = 4.05 NEE281 pKa = 4.27 KK282 pKa = 10.89 HH283 pKa = 6.58 PLTLLPQTVIQLKK296 pKa = 10.59 LLFEE300 pKa = 5.28 SIDD303 pKa = 3.41
Molecular weight: 35.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.483
IPC2_protein 4.507
IPC_protein 4.469
Toseland 4.304
ProMoST 4.558
Dawson 4.431
Bjellqvist 4.596
Wikipedia 4.329
Rodwell 4.317
Grimsley 4.215
Solomon 4.431
Lehninger 4.393
Nozaki 4.546
DTASelect 4.736
Thurlkill 4.317
EMBOSS 4.342
Sillero 4.596
Patrickios 3.605
IPC_peptide 4.431
IPC2_peptide 4.584
IPC2.peptide.svr19 4.515
Protein with the highest isoelectric point:
>tr|F1DAS9|F1DAS9_9VIRU Uncharacterized protein OS=Maverick-related virus strain Spezl OX=1932931 GN=MV08 PE=4 SV=1
MM1 pKa = 6.31 THH3 pKa = 6.04 KK4 pKa = 9.59 TSKK7 pKa = 10.19 NKK9 pKa = 10.23 RR10 pKa = 11.84 KK11 pKa = 9.91 ASPIRR16 pKa = 11.84 LMPRR20 pKa = 11.84 NIYY23 pKa = 9.49 QEE25 pKa = 3.88 QKK27 pKa = 9.87 AAAIIDD33 pKa = 3.54 PEE35 pKa = 4.09 EE36 pKa = 4.88 RR37 pKa = 11.84 YY38 pKa = 9.41 EE39 pKa = 3.97 ALKK42 pKa = 10.43 KK43 pKa = 10.19 IRR45 pKa = 11.84 LTTDD49 pKa = 2.34 QRR51 pKa = 11.84 MTKK54 pKa = 10.45 EE55 pKa = 4.04 EE56 pKa = 4.0 IKK58 pKa = 10.68 KK59 pKa = 9.44 LRR61 pKa = 11.84 NMINSDD67 pKa = 4.25 IIFMSS72 pKa = 3.64
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.114
IPC2_protein 9.428
IPC_protein 9.458
Toseland 10.467
ProMoST 9.955
Dawson 10.555
Bjellqvist 10.131
Wikipedia 10.657
Rodwell 11.14
Grimsley 10.584
Solomon 10.584
Lehninger 10.57
Nozaki 10.409
DTASelect 10.131
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.452
Patrickios 10.906
IPC_peptide 10.599
IPC2_peptide 8.507
IPC2.peptide.svr19 8.546
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
5867
54
712
293.4
33.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.346 ± 0.591
0.972 ± 0.152
6.63 ± 0.233
6.835 ± 0.532
4.568 ± 0.303
5.13 ± 0.509
1.619 ± 0.165
6.784 ± 0.312
11.181 ± 0.838
9.357 ± 0.438
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.909 ± 0.236
7.346 ± 0.447
4.295 ± 0.461
3.665 ± 0.187
3.971 ± 0.269
6.017 ± 0.46
5.659 ± 0.424
4.943 ± 0.381
0.614 ± 0.084
4.159 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here