Natrarchaeobaculum sulfurireducens
Average proteome isoelectric point is 4.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3734 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346PQM8|A0A346PQM8_9EURY Uncharacterized protein OS=Natrarchaeobaculum sulfurireducens OX=2044521 GN=AArcMg_1816 PE=4 SV=1
MM1 pKa = 7.49 SDD3 pKa = 2.89 EE4 pKa = 3.86 RR5 pKa = 11.84 RR6 pKa = 11.84 YY7 pKa = 10.53 ASRR10 pKa = 11.84 RR11 pKa = 11.84 KK12 pKa = 9.74 LLAATGSLTALGLAGCLGDD31 pKa = 4.26 EE32 pKa = 4.89 EE33 pKa = 5.75 GDD35 pKa = 3.81 EE36 pKa = 4.82 PEE38 pKa = 4.78 PADD41 pKa = 5.94 DD42 pKa = 6.13 GDD44 pKa = 5.37 DD45 pKa = 3.98 DD46 pKa = 6.42 HH47 pKa = 9.36 DD48 pKa = 6.87 DD49 pKa = 4.85 DD50 pKa = 6.27 DD51 pKa = 5.85 HH52 pKa = 9.08 DD53 pKa = 5.79 HH54 pKa = 7.79 DD55 pKa = 6.51 DD56 pKa = 5.3 DD57 pKa = 5.59 HH58 pKa = 8.97 DD59 pKa = 5.46 HH60 pKa = 7.78 DD61 pKa = 6.31 DD62 pKa = 4.16 GVADD66 pKa = 4.58 EE67 pKa = 5.24 YY68 pKa = 11.4 FDD70 pKa = 3.9 EE71 pKa = 6.02 LGVADD76 pKa = 4.72 EE77 pKa = 5.14 GFVILDD83 pKa = 3.68 RR84 pKa = 11.84 AHH86 pKa = 7.2 DD87 pKa = 3.67 PHH89 pKa = 8.28 EE90 pKa = 4.33 EE91 pKa = 3.7 VAYY94 pKa = 9.99 VHH96 pKa = 6.84 GDD98 pKa = 2.82 HH99 pKa = 6.46 WHH101 pKa = 6.98 GDD103 pKa = 3.65 LPSIPVDD110 pKa = 3.7 DD111 pKa = 4.34 NVSIGAEE118 pKa = 3.89 IEE120 pKa = 4.76 LEE122 pKa = 4.58 DD123 pKa = 5.02 GDD125 pKa = 4.34 EE126 pKa = 4.59 LEE128 pKa = 5.96 LGDD131 pKa = 4.14 EE132 pKa = 4.48 YY133 pKa = 11.14 EE134 pKa = 4.39 LRR136 pKa = 11.84 VAVAPDD142 pKa = 3.46 EE143 pKa = 4.52 PEE145 pKa = 4.03 GVVGIDD151 pKa = 3.04 EE152 pKa = 4.89 DD153 pKa = 5.36 DD154 pKa = 3.46 YY155 pKa = 11.78 HH156 pKa = 7.73 GDD158 pKa = 3.29 HH159 pKa = 5.82 VHH161 pKa = 6.09 IHH163 pKa = 6.36 GEE165 pKa = 4.1 QEE167 pKa = 4.12 GVTEE171 pKa = 4.25 VVFMIWHH178 pKa = 7.56 DD179 pKa = 3.72 DD180 pKa = 3.48 HH181 pKa = 9.02 ADD183 pKa = 3.67 YY184 pKa = 10.87 QSPPITAQVVEE195 pKa = 4.56 EE196 pKa = 5.19 DD197 pKa = 4.31 DD198 pKa = 5.52 DD199 pKa = 5.14 HH200 pKa = 8.92 DD201 pKa = 4.84 HH202 pKa = 7.4 DD203 pKa = 6.05 HH204 pKa = 7.4 DD205 pKa = 4.52 DD206 pKa = 3.92 EE207 pKa = 4.69 YY208 pKa = 11.94 VSEE211 pKa = 4.31 FQLLDD216 pKa = 3.58 RR217 pKa = 11.84 AHH219 pKa = 7.27 DD220 pKa = 3.57 PHH222 pKa = 8.4 EE223 pKa = 4.86 EE224 pKa = 3.41 ISYY227 pKa = 8.76 VHH229 pKa = 6.82 GDD231 pKa = 3.02 HH232 pKa = 6.56 WHH234 pKa = 6.74 GEE236 pKa = 4.14 DD237 pKa = 4.97 DD238 pKa = 4.5 FPTIPVGDD246 pKa = 3.86 NVSIGAEE253 pKa = 4.08 LEE255 pKa = 4.24 DD256 pKa = 4.31 ADD258 pKa = 4.49 GDD260 pKa = 4.4 EE261 pKa = 4.53 IVLGPEE267 pKa = 3.85 YY268 pKa = 10.65 EE269 pKa = 4.43 LGVEE273 pKa = 4.14 LADD276 pKa = 3.96 GAEE279 pKa = 4.08 EE280 pKa = 4.42 GVVEE284 pKa = 4.13 IDD286 pKa = 3.85 EE287 pKa = 5.15 DD288 pKa = 3.85 EE289 pKa = 5.44 DD290 pKa = 3.89 YY291 pKa = 11.42 HH292 pKa = 8.16 GDD294 pKa = 3.4 HH295 pKa = 5.84 VHH297 pKa = 6.05 IHH299 pKa = 6.3 GEE301 pKa = 4.25 SAGEE305 pKa = 4.15 TEE307 pKa = 4.85 VVFTLIHH314 pKa = 7.19 DD315 pKa = 3.82 DD316 pKa = 3.61 HH317 pKa = 8.73 VDD319 pKa = 3.59 YY320 pKa = 7.47 EE321 pKa = 4.89 TPALTVTVGDD331 pKa = 4.27 DD332 pKa = 3.05
Molecular weight: 36.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.567
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.91
Patrickios 1.1
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A346PSJ3|A0A346PSJ3_9EURY Phage protein OS=Natrarchaeobaculum sulfurireducens OX=2044521 GN=AArc1_1230 PE=3 SV=1
MM1 pKa = 8.01 PYY3 pKa = 10.03 RR4 pKa = 11.84 VALEE8 pKa = 3.88 KK9 pKa = 10.89 LVFDD13 pKa = 4.21 RR14 pKa = 11.84 FGRR17 pKa = 11.84 PIVRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 APVPDD28 pKa = 3.9 TNSLIDD34 pKa = 3.38 ASRR37 pKa = 11.84 FLVRR41 pKa = 11.84 VGSTDD46 pKa = 3.41 EE47 pKa = 4.09 PSEE50 pKa = 4.29 PFDD53 pKa = 4.15 SRR55 pKa = 11.84 RR56 pKa = 11.84 PTT58 pKa = 3.17
Molecular weight: 6.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.428
IPC_protein 10.672
Toseland 10.687
ProMoST 10.789
Dawson 10.76
Bjellqvist 10.613
Wikipedia 11.096
Rodwell 10.599
Grimsley 10.818
Solomon 11.052
Lehninger 10.994
Nozaki 10.657
DTASelect 10.613
Thurlkill 10.687
EMBOSS 11.125
Sillero 10.716
Patrickios 10.496
IPC_peptide 11.052
IPC2_peptide 9.823
IPC2.peptide.svr19 8.825
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3734
0
3734
1078029
44
2660
288.7
31.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.455 ± 0.053
0.766 ± 0.014
8.75 ± 0.057
9.244 ± 0.05
3.228 ± 0.027
8.137 ± 0.038
2.033 ± 0.02
4.385 ± 0.031
1.779 ± 0.026
8.93 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.729 ± 0.017
2.243 ± 0.021
4.557 ± 0.028
2.456 ± 0.02
6.42 ± 0.048
5.596 ± 0.033
6.491 ± 0.031
9.026 ± 0.042
1.112 ± 0.016
2.664 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here