Hansschlegelia quercus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Hansschlegelia

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9GQ44|A0A4Q9GQ44_9RHIZ Putative fluoride ion transporter CrcB OS=Hansschlegelia quercus OX=2528245 GN=crcB PE=3 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.78VVTDD7 pKa = 2.96NCIRR11 pKa = 11.84CKK13 pKa = 11.03YY14 pKa = 7.82MDD16 pKa = 4.38CVEE19 pKa = 4.32VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.21NMLVIHH36 pKa = 7.24PDD38 pKa = 2.97EE39 pKa = 5.88CIDD42 pKa = 4.44CGVCEE47 pKa = 4.44PEE49 pKa = 5.05CPAEE53 pKa = 4.43AIKK56 pKa = 10.66PDD58 pKa = 3.73TDD60 pKa = 3.45SGLEE64 pKa = 3.92SWVKK68 pKa = 10.97LNGDD72 pKa = 3.95YY73 pKa = 11.03AKK75 pKa = 9.92SWPNITIKK83 pKa = 10.39RR84 pKa = 11.84DD85 pKa = 3.51PPPDD89 pKa = 3.7AKK91 pKa = 11.13EE92 pKa = 3.75MDD94 pKa = 3.76GVEE97 pKa = 4.39GKK99 pKa = 10.22LEE101 pKa = 4.39KK102 pKa = 10.94YY103 pKa = 10.42FSPEE107 pKa = 3.79PGEE110 pKa = 4.27GDD112 pKa = 3.12

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9GI89|A0A4Q9GI89_9RHIZ Translation initiation factor IF-2 OS=Hansschlegelia quercus OX=2528245 GN=infB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.24IIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3283

0

3283

1060935

26

2867

323.2

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.914 ± 0.063

0.739 ± 0.012

5.781 ± 0.03

5.941 ± 0.037

3.66 ± 0.023

8.902 ± 0.043

1.79 ± 0.02

4.694 ± 0.028

3.259 ± 0.035

10.008 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.019

2.173 ± 0.021

5.321 ± 0.031

2.613 ± 0.025

7.516 ± 0.042

5.366 ± 0.027

5.046 ± 0.025

7.831 ± 0.035

1.235 ± 0.017

2.027 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski