Vallicoccus soli
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3658 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3A3ZCU1|A0A3A3ZCU1_9ACTN GAF domain-containing protein OS=Vallicoccus soli OX=2339232 GN=D5H78_18240 PE=4 SV=1
MM1 pKa = 7.72 GSRR4 pKa = 11.84 TNRR7 pKa = 11.84 PAALAAGVLGLSLVLTACGSDD28 pKa = 2.94 NGGDD32 pKa = 3.69 DD33 pKa = 3.72 TASGGSGGGSGADD46 pKa = 3.16 CSAYY50 pKa = 9.76 EE51 pKa = 4.84 QYY53 pKa = 9.6 TQDD56 pKa = 3.98 FPGIDD61 pKa = 3.38 GKK63 pKa = 8.7 TVSVYY68 pKa = 10.13 TSIVTPEE75 pKa = 4.34 DD76 pKa = 3.53 QPHH79 pKa = 6.74 LDD81 pKa = 3.15 SYY83 pKa = 11.92 AAFEE87 pKa = 4.43 EE88 pKa = 4.86 CTGVDD93 pKa = 3.37 VQYY96 pKa = 11.04 EE97 pKa = 4.03 GDD99 pKa = 3.56 KK100 pKa = 11.34 AFEE103 pKa = 4.06 AQVLVRR109 pKa = 11.84 AKK111 pKa = 10.57 AGNAPDD117 pKa = 3.46 IAYY120 pKa = 9.95 VPQPGLLQQLVATGAAVPAPEE141 pKa = 3.72 QTAANVDD148 pKa = 3.76 EE149 pKa = 5.69 FFGEE153 pKa = 4.03 DD154 pKa = 2.72 WKK156 pKa = 11.46 GYY158 pKa = 10.47 GSVDD162 pKa = 3.33 GQFYY166 pKa = 10.39 AAPLGANVKK175 pKa = 10.29 SFVWYY180 pKa = 10.25 SPSEE184 pKa = 4.2 FEE186 pKa = 4.42 DD187 pKa = 3.08 SGYY190 pKa = 10.79 EE191 pKa = 4.01 VPTTLDD197 pKa = 3.43 EE198 pKa = 4.81 LKK200 pKa = 10.88 SLTDD204 pKa = 3.38 QMVADD209 pKa = 5.65 GKK211 pKa = 10.73 VPWCAGIASGEE222 pKa = 4.07 ATGWPVTDD230 pKa = 3.15 WMEE233 pKa = 5.81 DD234 pKa = 2.78 MMLRR238 pKa = 11.84 LNGPEE243 pKa = 5.81 VYY245 pKa = 10.11 DD246 pKa = 2.92 QWVNHH251 pKa = 6.9 EE252 pKa = 4.35 IPFNGPEE259 pKa = 3.82 STAALDD265 pKa = 4.35 AVGEE269 pKa = 4.11 YY270 pKa = 10.41 LKK272 pKa = 11.08 NPEE275 pKa = 4.41 YY276 pKa = 11.46 VNGGYY281 pKa = 11.09 GDD283 pKa = 3.7 VTTIATTTFQDD294 pKa = 3.06 GGLPIIDD301 pKa = 4.33 GACSLHH307 pKa = 6.07 RR308 pKa = 11.84 QAGFYY313 pKa = 9.29 AANWPEE319 pKa = 3.99 GTEE322 pKa = 3.98 VAEE325 pKa = 5.08 DD326 pKa = 3.8 GDD328 pKa = 3.98 VFAFYY333 pKa = 10.95 LPGQTAEE340 pKa = 4.41 DD341 pKa = 4.06 RR342 pKa = 11.84 PVLGGGEE349 pKa = 4.04 FVLAFDD355 pKa = 5.84 DD356 pKa = 4.14 RR357 pKa = 11.84 PEE359 pKa = 3.96 VQAFQTFLSSDD370 pKa = 2.5 TWANEE375 pKa = 3.67 KK376 pKa = 10.68 AIATPQGGWVSANSGLEE393 pKa = 3.67 VDD395 pKa = 4.66 NLVSPIDD402 pKa = 3.62 QLSAEE407 pKa = 4.47 ILQDD411 pKa = 3.4 PNAVFRR417 pKa = 11.84 FDD419 pKa = 5.93 GSDD422 pKa = 3.44 QMPGAVGADD431 pKa = 3.73 AFWKK435 pKa = 10.38 QATNWITGQSTQEE448 pKa = 4.02 TVDD451 pKa = 3.93 KK452 pKa = 11.25 IEE454 pKa = 4.4 AAWPASS460 pKa = 3.41
Molecular weight: 48.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.872
Patrickios 1.316
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A3A3YTM2|A0A3A3YTM2_9ACTN Iron-sulfur protein OS=Vallicoccus soli OX=2339232 GN=D5H78_13320 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 8.58 THH17 pKa = 5.46 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.75 GRR40 pKa = 11.84 ASLSAA45 pKa = 3.83
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3658
0
3658
1198445
29
1814
327.6
34.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.525 ± 0.077
0.688 ± 0.01
6.11 ± 0.03
5.652 ± 0.04
2.155 ± 0.027
10.35 ± 0.041
2.012 ± 0.021
1.698 ± 0.031
1.119 ± 0.021
11.139 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.336 ± 0.014
1.09 ± 0.018
6.506 ± 0.034
2.545 ± 0.021
9.159 ± 0.053
4.228 ± 0.03
5.177 ± 0.03
10.338 ± 0.037
1.433 ± 0.015
1.741 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here