Seleniivibrio woodruffii
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2684 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R1KDS5|A0A4R1KDS5_9BACT 50S ribosomal protein L5 OS=Seleniivibrio woodruffii OX=1078050 GN=rplE PE=3 SV=1
MM1 pKa = 7.63 SSFKK5 pKa = 10.55 QVSFVLNNVEE15 pKa = 5.11 NYY17 pKa = 9.3 QQLAAEE23 pKa = 4.75 AGANSEE29 pKa = 4.11 VFIINSEE36 pKa = 4.03 LDD38 pKa = 3.3 ALEE41 pKa = 3.87 QMAEE45 pKa = 3.85 ILQDD49 pKa = 3.84 YY50 pKa = 10.69 SDD52 pKa = 5.36 LDD54 pKa = 3.63 ALHH57 pKa = 6.95 IISHH61 pKa = 6.49 GSSGVINLGSVQLSDD76 pKa = 3.6 TNINEE81 pKa = 3.91 YY82 pKa = 10.79 SAVLSVIGSSLSEE95 pKa = 3.97 EE96 pKa = 4.31 GDD98 pKa = 3.22 ILVYY102 pKa = 10.22 GCNVASGYY110 pKa = 9.45 EE111 pKa = 4.35 GKK113 pKa = 10.51 SLIASLAQATGADD126 pKa = 3.33 IAASEE131 pKa = 4.69 DD132 pKa = 3.42 LTGAADD138 pKa = 4.71 KK139 pKa = 11.5 GGDD142 pKa = 3.04 WVLEE146 pKa = 4.09 FRR148 pKa = 11.84 SAEE151 pKa = 3.99 INVDD155 pKa = 3.8 SVSAEE160 pKa = 3.9 YY161 pKa = 10.76 AGVLDD166 pKa = 3.78 SVTYY170 pKa = 9.26 TFEE173 pKa = 4.38 VEE175 pKa = 4.0 DD176 pKa = 4.57 PLWSVLSTVSGGFADD191 pKa = 5.97 GYY193 pKa = 9.86 PSSLKK198 pKa = 10.74 YY199 pKa = 10.3 SAPDD203 pKa = 3.21 GNTLVFTLDD212 pKa = 3.93 DD213 pKa = 4.72 PDD215 pKa = 3.62 NAGFSIFQPMTGGEE229 pKa = 4.22 LLWSGEE235 pKa = 3.75 NDD237 pKa = 3.47 SYY239 pKa = 11.62 SSHH242 pKa = 6.62 PHH244 pKa = 6.06 SISIAMEE251 pKa = 4.06 GGGAFNPTSIKK262 pKa = 10.54 AAWDD266 pKa = 3.21 AAGNYY271 pKa = 8.99 IGNPVDD277 pKa = 4.29 GRR279 pKa = 11.84 PTPDD283 pKa = 2.78 ITVTAYY289 pKa = 10.6 DD290 pKa = 4.12 EE291 pKa = 4.65 NGDD294 pKa = 4.53 AIPGKK299 pKa = 10.28 SVTIHH304 pKa = 7.25 DD305 pKa = 3.94 VTSPQGFVTFDD316 pKa = 3.51 LSSFGSVYY324 pKa = 10.42 KK325 pKa = 10.43 IVVSTTGADD334 pKa = 3.58 LMLDD338 pKa = 4.17 DD339 pKa = 5.89 LVLDD343 pKa = 4.01 NTPAPSDD350 pKa = 3.45 TALLTFEE357 pKa = 5.66 AGDD360 pKa = 3.85 GTTSGLGTKK369 pKa = 8.28 TVTYY373 pKa = 10.25 DD374 pKa = 3.36 DD375 pKa = 4.02 TGSGVEE381 pKa = 4.18 FTVEE385 pKa = 3.71 ATASQIISSSVGGAPDD401 pKa = 3.31 GRR403 pKa = 11.84 ADD405 pKa = 4.33 AEE407 pKa = 3.93 ALYY410 pKa = 10.35 FGWDD414 pKa = 3.36 EE415 pKa = 4.57 KK416 pKa = 10.25 EE417 pKa = 4.07 TSIKK421 pKa = 10.68 VSVEE425 pKa = 3.61 SGKK428 pKa = 10.71 AFDD431 pKa = 4.81 LVSFYY436 pKa = 10.86 ISNQNGAGNEE446 pKa = 4.14 DD447 pKa = 3.64 FTITTDD453 pKa = 2.97 SGGSYY458 pKa = 9.3 TFTFIDD464 pKa = 3.42 TGSITKK470 pKa = 10.11 QLVTIPAGDD479 pKa = 3.78 ADD481 pKa = 4.43 FNGITWFTITSPAGGAFIEE500 pKa = 4.49 LDD502 pKa = 3.79 NISVEE507 pKa = 4.46 NITAAAADD515 pKa = 3.96 AAPTLAATGATPSYY529 pKa = 11.29 AEE531 pKa = 4.36 GSTTGVDD538 pKa = 3.46 LFSSVTADD546 pKa = 3.53 TNDD549 pKa = 3.25 SGQVFTGFIVTVTNVSDD566 pKa = 3.36 TTEE569 pKa = 3.85 YY570 pKa = 10.24 LTIGGTDD577 pKa = 3.15 VALTNGNSVAVSGGTASVSIAAGTATVTVSGVSLANAAMGTLIDD621 pKa = 4.99 GITYY625 pKa = 10.74 KK626 pKa = 10.95 NLDD629 pKa = 3.5 TTATNGNRR637 pKa = 11.84 VVTVTQITDD646 pKa = 3.67 DD647 pKa = 4.04 GSSSNTAAPNISATVNVSGPIDD669 pKa = 3.18 ITFDD673 pKa = 3.72 FEE675 pKa = 6.13 ANTSPVSDD683 pKa = 3.7 DD684 pKa = 2.96 TGATVDD690 pKa = 3.63 STATQTKK697 pKa = 9.3 NGEE700 pKa = 4.26 TIEE703 pKa = 4.14 IVSTGGDD710 pKa = 3.37 FSVGAEE716 pKa = 3.96 SAIFGMDD723 pKa = 2.87 IGAGFSGDD731 pKa = 3.1 ILSFDD736 pKa = 4.17 MMDD739 pKa = 3.66 GDD741 pKa = 5.36 LDD743 pKa = 3.79 SEE745 pKa = 4.61 YY746 pKa = 11.11 ASTRR750 pKa = 11.84 IDD752 pKa = 3.06 VDD754 pKa = 4.0 LQSGKK759 pKa = 10.49 YY760 pKa = 7.13 FTLKK764 pKa = 10.48 SISIFDD770 pKa = 4.04 NYY772 pKa = 10.89 NVDD775 pKa = 5.75 HH776 pKa = 7.31 IGQDD780 pKa = 3.03 IKK782 pKa = 10.94 IIASNGTEE790 pKa = 3.89 YY791 pKa = 10.77 TVVIDD796 pKa = 5.59 YY797 pKa = 10.97 EE798 pKa = 5.27 GDD800 pKa = 3.59 DD801 pKa = 3.8 NGGVTVDD808 pKa = 3.65 LSGVAGFDD816 pKa = 3.98 NITSFYY822 pKa = 10.21 IYY824 pKa = 11.01 ANGLPMQLVIDD835 pKa = 4.58 NIEE838 pKa = 4.07 LVNISSTPVLTAPTLSATGEE858 pKa = 4.32 TPTFTEE864 pKa = 4.77 GGSDD868 pKa = 3.18 VDD870 pKa = 5.41 LFDD873 pKa = 4.84 TVTADD878 pKa = 3.96 TNDD881 pKa = 3.3 SGQDD885 pKa = 3.15 FTGFVLTVTNVADD898 pKa = 3.58 TTEE901 pKa = 4.03 YY902 pKa = 10.17 LTIGGTDD909 pKa = 3.15 VALTNGNSVSVSGGTASVSISGGTATVTVTGLNLNDD945 pKa = 3.79 SAFSSLIDD953 pKa = 3.25 GMTYY957 pKa = 10.95 KK958 pKa = 9.51 NTSGSVTIGNRR969 pKa = 11.84 VITVTQVTDD978 pKa = 3.36 EE979 pKa = 4.46 GSSNNTYY986 pKa = 10.67 SPNITSTVTVADD998 pKa = 4.11 SVSSLPYY1005 pKa = 10.03 TILLRR1010 pKa = 11.84 LTDD1013 pKa = 3.65 TLNANFNTMAGYY1025 pKa = 10.59 NIDD1028 pKa = 3.76 GDD1030 pKa = 4.05 KK1031 pKa = 11.12 GSAIADD1037 pKa = 3.42 SRR1039 pKa = 11.84 YY1040 pKa = 10.9 AMPDD1044 pKa = 3.07 RR1045 pKa = 11.84 LSSNMLVHH1053 pKa = 6.8 GNLIYY1058 pKa = 10.8 AVIYY1062 pKa = 10.32 DD1063 pKa = 3.99 NNANLYY1069 pKa = 8.87 ATFAKK1074 pKa = 10.3 FDD1076 pKa = 3.7 GVDD1079 pKa = 3.76 FTFYY1083 pKa = 11.03 EE1084 pKa = 5.4 GITFSAVSTMVAADD1098 pKa = 4.21 DD1099 pKa = 4.19 GDD1101 pKa = 4.19 VYY1103 pKa = 11.06 MLATASTDD1111 pKa = 2.85 SSKK1114 pKa = 11.35 YY1115 pKa = 9.77 YY1116 pKa = 10.4 LYY1118 pKa = 10.96 KK1119 pKa = 10.41 FDD1121 pKa = 4.47 AQTEE1125 pKa = 4.55 TVSKK1129 pKa = 10.67 VIDD1132 pKa = 4.46 IISHH1136 pKa = 4.48 QSIIFEE1142 pKa = 4.2 YY1143 pKa = 11.03 ADD1145 pKa = 3.2 GKK1147 pKa = 11.33 LYY1149 pKa = 9.65 FACNEE1154 pKa = 3.76 ASGSYY1159 pKa = 10.12 ISGGVYY1165 pKa = 9.76 TYY1167 pKa = 11.28 DD1168 pKa = 3.32 GTTVSALTISGGSTILGAPSNQSSYY1193 pKa = 9.89 VVEE1196 pKa = 4.81 FNGTTYY1202 pKa = 10.51 IVGSEE1207 pKa = 4.25 TVGGNSIKK1215 pKa = 10.62 NIYY1218 pKa = 9.76 KK1219 pKa = 9.8 IDD1221 pKa = 3.73 GSGNAVKK1228 pKa = 9.38 VTSNANQYY1236 pKa = 8.77 LHH1238 pKa = 7.23 PGQMCEE1244 pKa = 3.96 YY1245 pKa = 10.1 NGRR1248 pKa = 11.84 LYY1250 pKa = 9.97 FTLTDD1255 pKa = 4.07 LTPSSEE1261 pKa = 3.78 TSYY1264 pKa = 10.78 LYY1266 pKa = 10.82 SIGTGASEE1274 pKa = 4.32 TPQLEE1279 pKa = 4.25 WAGTRR1284 pKa = 11.84 VYY1286 pKa = 10.57 QATVLNGEE1294 pKa = 4.51 LYY1296 pKa = 10.56 VVGGASAYY1304 pKa = 10.63 ALDD1307 pKa = 4.21 LYY1309 pKa = 10.94 KK1310 pKa = 10.69 YY1311 pKa = 10.46 DD1312 pKa = 3.37 GSAFNKK1318 pKa = 7.63 ITNINTANNAYY1329 pKa = 10.3 GITNLTYY1336 pKa = 10.29 HH1337 pKa = 7.14 DD1338 pKa = 3.67 GKK1340 pKa = 10.84 IYY1342 pKa = 10.52 FLATAGIDD1350 pKa = 3.64 NLRR1353 pKa = 11.84 SLYY1356 pKa = 10.6 AYY1358 pKa = 10.19 DD1359 pKa = 3.72 GTSTEE1364 pKa = 5.09 LIVSYY1369 pKa = 10.69 TDD1371 pKa = 3.53 LGGSGIRR1378 pKa = 11.84 NEE1380 pKa = 4.51 LVNDD1384 pKa = 4.42 IYY1386 pKa = 11.47 NSTPLDD1392 pKa = 3.28 IFNGNMAAFLTGARR1406 pKa = 11.84 GIVYY1410 pKa = 10.2 GEE1412 pKa = 4.16 SGDD1415 pKa = 4.22 VITVAPNLVITDD1427 pKa = 3.33 VDD1429 pKa = 3.92 GNNITGATVEE1439 pKa = 4.34 FSEE1442 pKa = 5.13 GFQAGDD1448 pKa = 3.45 LLAITEE1454 pKa = 4.24 ASGITASYY1462 pKa = 10.53 NSSTGVLTLSGTATIAEE1479 pKa = 4.3 YY1480 pKa = 10.58 QAVLRR1485 pKa = 11.84 TLEE1488 pKa = 3.96 LTAADD1493 pKa = 3.81 EE1494 pKa = 4.47 TGDD1497 pKa = 3.48 RR1498 pKa = 11.84 VLLLRR1503 pKa = 11.84 VTDD1506 pKa = 4.04 EE1507 pKa = 4.4 SGTTSSATNLNAFASVAIADD1527 pKa = 4.26 SEE1529 pKa = 4.75 SEE1531 pKa = 4.3 TVIVDD1536 pKa = 4.29 FASMPTGLAEE1546 pKa = 4.33 SGWAGEE1552 pKa = 4.36 CSVASSNLDD1561 pKa = 3.43 NIKK1564 pKa = 10.79 FIMTASAVGTSLGSIVTQSDD1584 pKa = 3.45 SGGGLQYY1591 pKa = 11.3 NATDD1595 pKa = 3.46 DD1596 pKa = 3.36 TMYY1599 pKa = 11.4 ASVNNAWDD1607 pKa = 3.45 LTTIRR1612 pKa = 11.84 NVMSLVIASEE1622 pKa = 4.02 NGEE1625 pKa = 4.01 SFEE1628 pKa = 4.11 FHH1630 pKa = 6.51 GFTVKK1635 pKa = 10.58 GSVMSLSGMNIVGYY1649 pKa = 10.54 KK1650 pKa = 10.53 DD1651 pKa = 3.64 GVQVAQQSVAALSNSVNNIVLSSDD1675 pKa = 3.34 FDD1677 pKa = 4.1 DD1678 pKa = 5.26 VDD1680 pKa = 4.18 VVWITRR1686 pKa = 11.84 VAGTFRR1692 pKa = 11.84 AQFDD1696 pKa = 3.75 DD1697 pKa = 5.16 FIIIEE1702 pKa = 4.33 KK1703 pKa = 10.22 VSSAFAAPTLSATGATPVFDD1723 pKa = 4.04 EE1724 pKa = 4.83 ASGTVVDD1731 pKa = 5.24 LFSTVTADD1739 pKa = 3.64 TNDD1742 pKa = 3.34 SGQVFTGLKK1751 pKa = 9.07 LTVTNVADD1759 pKa = 3.58 TTEE1762 pKa = 4.08 YY1763 pKa = 10.29 LTIDD1767 pKa = 3.7 GTDD1770 pKa = 3.08 VALTNGNSVSVSGGTASVSISGGTATVTLTGFSLSNTDD1808 pKa = 2.95 MGTLVDD1814 pKa = 5.66 GITYY1818 pKa = 10.7 KK1819 pKa = 10.83 NDD1821 pKa = 3.29 DD1822 pKa = 3.68 STATAGNRR1830 pKa = 11.84 VVTITQITDD1839 pKa = 3.28 DD1840 pKa = 4.04 GASANTAALSISSTVTVRR1858 pKa = 11.84 EE1859 pKa = 3.94 PSITSATYY1867 pKa = 10.01 DD1868 pKa = 3.12 ASTGEE1873 pKa = 4.15 LVVTGAGLTAGDD1885 pKa = 4.96 SIDD1888 pKa = 3.79 TTKK1891 pKa = 10.32 LTITGEE1897 pKa = 4.16 GGNTYY1902 pKa = 8.68 TLAGSYY1908 pKa = 8.84 TVTASSATSFTVTLDD1923 pKa = 3.09 ATDD1926 pKa = 3.42 QLFVEE1931 pKa = 5.11 GLLNKK1936 pKa = 10.36 NGTSSAGSTTFNLGAAAEE1954 pKa = 4.31 WTAGAPADD1962 pKa = 3.74 STGNGVTVSNLSAPAISGVSYY1983 pKa = 11.01 NAFTGVLTVTGTNFVHH1999 pKa = 6.76 QPGAANDD2006 pKa = 3.52 IDD2008 pKa = 4.05 VSAFTFTGEE2017 pKa = 4.11 GGGTYY2022 pKa = 10.71 SLTNTSDD2029 pKa = 3.6 VEE2031 pKa = 4.3 ITSATSFTVTLSATDD2046 pKa = 3.52 EE2047 pKa = 4.32 AAVEE2051 pKa = 4.76 AILNANGTSSSDD2063 pKa = 3.16 STVYY2067 pKa = 10.93 NIAVADD2073 pKa = 3.7 NWNGPVTGGDD2083 pKa = 3.12 ISDD2086 pKa = 3.59 STTAVNVTGHH2096 pKa = 5.51 GTAPVLDD2103 pKa = 4.69 LNGGTAGTDD2112 pKa = 3.19 VTLTLGAAEE2121 pKa = 4.64 SGLAPSVVISDD2132 pKa = 3.77 VEE2134 pKa = 4.2 NDD2136 pKa = 3.11 VSGWDD2141 pKa = 3.62 GSTLTVQRR2149 pKa = 11.84 VDD2151 pKa = 2.94 SGGAADD2157 pKa = 4.78 GCAHH2161 pKa = 6.97 DD2162 pKa = 4.16 VFSFVSVDD2170 pKa = 3.27 NFVVNGSGITHH2181 pKa = 6.94 GADD2184 pKa = 3.34 SNGTLSTTGGTQFATWSYY2202 pKa = 7.25 TTADD2206 pKa = 3.62 GKK2208 pKa = 11.18 LLISFDD2214 pKa = 3.91 SDD2216 pKa = 2.79 ATTALVQEE2224 pKa = 4.67 VASSIGYY2231 pKa = 9.76 EE2232 pKa = 3.89 NATPYY2237 pKa = 10.9 GDD2239 pKa = 3.77 AVIEE2243 pKa = 4.15 FSLYY2247 pKa = 11.07 DD2248 pKa = 3.5 GMITTKK2254 pKa = 10.7 SEE2256 pKa = 3.9 VTVTSNIIHH2265 pKa = 5.38 VTQTDD2270 pKa = 3.71 YY2271 pKa = 10.37 DD2272 pKa = 4.12 TDD2274 pKa = 3.63 GDD2276 pKa = 4.23 AADD2279 pKa = 4.28 GLSLYY2284 pKa = 9.62 EE2285 pKa = 4.32 ALAIAKK2291 pKa = 10.39 DD2292 pKa = 3.38 GDD2294 pKa = 4.29 TILLHH2299 pKa = 6.74 DD2300 pKa = 4.48 GVYY2303 pKa = 10.27 RR2304 pKa = 11.84 GQFDD2308 pKa = 3.06 ITKK2311 pKa = 10.27 AVTIDD2316 pKa = 3.48 ALNGEE2321 pKa = 4.48 NGNVVIEE2328 pKa = 4.72 SPDD2331 pKa = 3.6 ADD2333 pKa = 4.51 DD2334 pKa = 5.24 LVQVLPDD2341 pKa = 3.17 MMTNNGRR2348 pKa = 11.84 WRR2350 pKa = 11.84 MPVLNVNTSGDD2361 pKa = 3.62 AGTVTIKK2368 pKa = 10.91 NITVDD2373 pKa = 4.14 GRR2375 pKa = 11.84 DD2376 pKa = 3.04 QGLADD2381 pKa = 4.16 GFANNKK2387 pKa = 9.43 DD2388 pKa = 3.61 LLGIGIVNSNVLIDD2402 pKa = 4.49 NVSLSNFRR2410 pKa = 11.84 CEE2412 pKa = 6.16 DD2413 pKa = 3.27 SGEE2416 pKa = 4.18 WGWGEE2421 pKa = 4.14 NFPIMAEE2428 pKa = 4.05 ADD2430 pKa = 3.46 SSLSSVVNVTIQNSDD2445 pKa = 2.33 ISNFQKK2451 pKa = 9.91 TGIVGWGPMLNLTVDD2466 pKa = 3.38 STTITGSGTAGVAGQNGIQVGSGGLRR2492 pKa = 11.84 TGTTATITNNTITNFGFLSSVYY2514 pKa = 9.84 AASGVLIVNAGDD2526 pKa = 3.5 LTITGNTFEE2535 pKa = 4.42 GTADD2539 pKa = 3.19 GSGRR2543 pKa = 11.84 FSGVGINDD2551 pKa = 3.47 VLNGTIEE2558 pKa = 4.26 MNVSGNTFTNADD2570 pKa = 3.59 YY2571 pKa = 11.22 GILNEE2576 pKa = 4.79 AYY2578 pKa = 7.62 ITHH2581 pKa = 7.24 ILTVGTNDD2589 pKa = 3.45 FSGATIAVHH2598 pKa = 6.82 DD2599 pKa = 4.82 SYY2601 pKa = 11.39 IIADD2605 pKa = 4.28 PSTEE2609 pKa = 4.15 LPFDD2613 pKa = 3.67 NATTISIHH2621 pKa = 5.45 STASPAVGAVAYY2633 pKa = 9.48 YY2634 pKa = 10.53 LYY2636 pKa = 11.0 DD2637 pKa = 4.54 GDD2639 pKa = 6.31 DD2640 pKa = 3.64 SFTDD2644 pKa = 3.4 TGSVDD2649 pKa = 3.48 SVVYY2653 pKa = 10.55 GGGGDD2658 pKa = 3.72 DD2659 pKa = 4.63 TITTGSGDD2667 pKa = 3.71 DD2668 pKa = 3.67 VLAGGLGDD2676 pKa = 3.95 DD2677 pKa = 4.5 TLTGGAGEE2685 pKa = 4.95 DD2686 pKa = 3.36 VFLFQAQEE2694 pKa = 4.26 TVPEE2698 pKa = 4.25 APTVVVYY2705 pKa = 10.68 DD2706 pKa = 3.43 ITDD2709 pKa = 4.08 FGVDD2713 pKa = 4.2 TITDD2717 pKa = 3.75 MTQSDD2722 pKa = 5.13 AIRR2725 pKa = 11.84 VTGADD2730 pKa = 3.55 FSDD2733 pKa = 4.48 HH2734 pKa = 6.51 IVLEE2738 pKa = 4.4 GDD2740 pKa = 3.56 GTNVEE2745 pKa = 4.19 AASMQVSYY2753 pKa = 11.43 DD2754 pKa = 3.12 SGTNTTSLFIDD2765 pKa = 3.53 TDD2767 pKa = 3.89 GSDD2770 pKa = 4.33 GADD2773 pKa = 3.37 LQINLSGEE2781 pKa = 4.4 FSASQFGFNGADD2793 pKa = 3.07 IFIGAPTLDD2802 pKa = 3.8 LNGAGGGTDD2811 pKa = 3.21 VSVTLASAATGLIPAVEE2828 pKa = 4.04 ISDD2831 pKa = 4.32 AEE2833 pKa = 3.99 NDD2835 pKa = 3.24 IAGWNGASLTVQRR2848 pKa = 11.84 VDD2850 pKa = 3.25 SSGNADD2856 pKa = 3.04 GSEE2859 pKa = 3.92 YY2860 pKa = 11.04 DD2861 pKa = 3.6 VFGFAAASGITVTGSITEE2879 pKa = 4.35 GADD2882 pKa = 3.46 SNGTLSSGGTSFATWSYY2899 pKa = 11.33 SSADD2903 pKa = 3.37 GKK2905 pKa = 9.6 LTIDD2909 pKa = 3.71 FNGNATTALVQSAAEE2924 pKa = 4.56 AISYY2928 pKa = 9.46 TNDD2931 pKa = 3.22 TPFGDD2936 pKa = 4.58 ANIQVTLDD2944 pKa = 3.46 DD2945 pKa = 4.0 GVRR2948 pKa = 11.84 TVTSEE2953 pKa = 3.89 VTVTSNIIHH2962 pKa = 5.25 VTQTDD2967 pKa = 3.67 FDD2969 pKa = 4.58 TDD2971 pKa = 3.01 GDD2973 pKa = 4.16 AADD2976 pKa = 4.29 GFSLAEE2982 pKa = 4.22 ALAMAQDD2989 pKa = 3.8 GDD2991 pKa = 4.17 TILIHH2996 pKa = 6.11 EE2997 pKa = 4.68 GVYY3000 pKa = 10.2 RR3001 pKa = 11.84 GQFSITKK3008 pKa = 9.79 AVTIDD3013 pKa = 3.35 AFQGGNVVIEE3023 pKa = 4.55 SPDD3026 pKa = 3.65 TADD3029 pKa = 3.57 LQAVNPTEE3037 pKa = 3.95 LTNNGRR3043 pKa = 11.84 WRR3045 pKa = 11.84 MPVINVDD3052 pKa = 3.79 TNGSAGTVIIKK3063 pKa = 10.59 NITIDD3068 pKa = 3.58 GRR3070 pKa = 11.84 DD3071 pKa = 3.58 QAVMDD3076 pKa = 4.9 LNNGSGDD3083 pKa = 3.78 FIGIGIANSNVVVDD3097 pKa = 5.04 GVTIKK3102 pKa = 10.76 NIRR3105 pKa = 11.84 SSDD3108 pKa = 3.04 SDD3110 pKa = 3.04 MWGEE3114 pKa = 4.1 SEE3116 pKa = 4.63 NYY3118 pKa = 10.27 GILAEE3123 pKa = 4.67 AGSALFTPVSVTVTNSDD3140 pKa = 2.89 IYY3142 pKa = 10.96 NYY3144 pKa = 10.17 QKK3146 pKa = 9.94 TGIVGWGPNLDD3157 pKa = 3.32 ITITDD3162 pKa = 3.41 NDD3164 pKa = 3.35 ITGAGVDD3171 pKa = 4.28 GTCGQNGIQIGSSNTDD3187 pKa = 2.65 RR3188 pKa = 11.84 YY3189 pKa = 9.35 GTTATITGNTISDD3202 pKa = 3.65 LGFEE3206 pKa = 4.37 NNVYY3210 pKa = 10.32 GATGIILRR3218 pKa = 11.84 QTGDD3222 pKa = 3.24 VTVSDD3227 pKa = 3.87 NTISGTVDD3235 pKa = 3.06 GSGNCAGVTVYY3246 pKa = 10.52 QGLNGVTITVEE3257 pKa = 4.0 DD3258 pKa = 3.7 NVFNNTDD3265 pKa = 3.31 YY3266 pKa = 11.61 AIFNEE3271 pKa = 4.69 FQSSGVILNVNGNDD3285 pKa = 3.57 FSGSVLAILDD3295 pKa = 3.78 SHH3297 pKa = 6.53 GLPDD3301 pKa = 4.18 TVWGNNTFGSSATSLTVNSSGTPMSGKK3328 pKa = 10.03 LSYY3331 pKa = 11.22 YY3332 pKa = 10.66 LFDD3335 pKa = 5.85 GDD3337 pKa = 5.32 DD3338 pKa = 3.71 TLTDD3342 pKa = 3.43 TGSVDD3347 pKa = 3.46 SVIYY3351 pKa = 10.57 GGGGNDD3357 pKa = 3.39 TLTAGSGDD3365 pKa = 4.39 DD3366 pKa = 3.66 ILVGGLGDD3374 pKa = 3.84 DD3375 pKa = 4.08 TLTGGDD3381 pKa = 3.92 GNDD3384 pKa = 2.96 VFLFEE3389 pKa = 4.19 AVEE3392 pKa = 4.2 YY3393 pKa = 9.94 YY3394 pKa = 10.09 PEE3396 pKa = 4.15 STTEE3400 pKa = 3.51 VMYY3403 pKa = 11.1 NVTDD3407 pKa = 4.54 FGTDD3411 pKa = 4.12 LIKK3414 pKa = 10.97 DD3415 pKa = 4.01 FSEE3418 pKa = 4.96 DD3419 pKa = 3.7 DD3420 pKa = 3.61 IIRR3423 pKa = 11.84 ISGADD3428 pKa = 3.61 FDD3430 pKa = 4.19 NHH3432 pKa = 6.18 NAVAGNGTNVLADD3445 pKa = 3.97 TMQVSYY3451 pKa = 11.42 DD3452 pKa = 3.41 SGTDD3456 pKa = 3.06 KK3457 pKa = 9.02 TTLYY3461 pKa = 10.45 IDD3463 pKa = 3.33 TDD3465 pKa = 4.61 GINGADD3471 pKa = 3.98 LVVKK3475 pKa = 10.74 LDD3477 pKa = 3.96 GEE3479 pKa = 4.69 FEE3481 pKa = 4.15 ATDD3484 pKa = 3.83 FLMSGTDD3491 pKa = 2.81 IRR3493 pKa = 11.84 YY3494 pKa = 7.98 EE3495 pKa = 4.14 VNVAPTVSLTALGGTSDD3512 pKa = 3.62 QTTTAPIALFDD3523 pKa = 4.49 ASAADD3528 pKa = 3.67 TGNNSQTFSSLTLTVEE3544 pKa = 4.3 GVEE3547 pKa = 4.45 DD3548 pKa = 3.39 AAEE3551 pKa = 4.15 YY3552 pKa = 10.41 IVIDD3556 pKa = 3.86 SVEE3559 pKa = 3.83 IALTNGNSVSVAGGTASVALVNGTATLVLSGLALSNSQILSLIDD3603 pKa = 3.64 SIAYY3607 pKa = 8.68 TNDD3610 pKa = 2.84 EE3611 pKa = 4.75 DD3612 pKa = 4.52 EE3613 pKa = 4.29 PTIGDD3618 pKa = 3.58 RR3619 pKa = 11.84 VITLTEE3625 pKa = 4.02 IKK3627 pKa = 10.62 DD3628 pKa = 3.54 SGNAANTVTPNIASTVSVNLYY3649 pKa = 10.24 SEE3651 pKa = 4.93 PEE3653 pKa = 4.14 LTVTGSDD3660 pKa = 3.22 VTFTEE3665 pKa = 4.13 KK3666 pKa = 11.33 GEE3668 pKa = 4.24 DD3669 pKa = 3.38 AVSLFEE3675 pKa = 4.15 ITAADD3680 pKa = 3.83 TGDD3683 pKa = 3.37 SGQTFTGIRR3692 pKa = 11.84 LTVTNVADD3700 pKa = 3.43 ATEE3703 pKa = 4.01 YY3704 pKa = 11.1 LVIGGAEE3711 pKa = 3.91 VALVNGTSVSVAGGTASVTVVNGTATLVLSGIDD3744 pKa = 3.64 LDD3746 pKa = 4.18 NTEE3749 pKa = 3.96 MAALVGGISYY3759 pKa = 10.82 LNTDD3763 pKa = 3.57 TTLTAGDD3770 pKa = 3.68 RR3771 pKa = 11.84 VITITEE3777 pKa = 4.15 VTDD3780 pKa = 3.21 SGTVSNVSTPNVSATVSVEE3799 pKa = 4.19 DD3800 pKa = 4.89 LFTIPTVTVAAAGGTLAYY3818 pKa = 10.3 AATTPVDD3825 pKa = 3.56 LFGTVTSADD3834 pKa = 3.41 TGDD3837 pKa = 3.62 EE3838 pKa = 4.4 GEE3840 pKa = 4.72 SFTGLKK3846 pKa = 9.25 LTVTNVSDD3854 pKa = 3.47 STEE3857 pKa = 4.03 IITIGGVDD3865 pKa = 3.46 VALTNGTSVSVAGGTASVTVVNGTATVTLTGLDD3898 pKa = 3.39 LDD3900 pKa = 4.13 AAEE3903 pKa = 5.5 LGTLVDD3909 pKa = 3.99 GAAYY3913 pKa = 9.52 KK3914 pKa = 9.31 NTGITPTGGNRR3925 pKa = 11.84 VVTLTEE3931 pKa = 4.0 ISDD3934 pKa = 3.72 SGAEE3938 pKa = 4.21 NNTVTPNVSSTVSVTASPAITNAAFNAAAGVITVTGTGLAAGDD3981 pKa = 4.14 VIDD3984 pKa = 4.09 ATKK3987 pKa = 9.56 LTLTGEE3993 pKa = 4.42 GGNTYY3998 pKa = 8.73 TLAGTYY4004 pKa = 8.49 TVAATANGFSLTLTEE4019 pKa = 4.12 TDD4021 pKa = 3.58 LLNVKK4026 pKa = 10.45 GILNSNGTTAGGSGTFNLAAASGWSVTKK4054 pKa = 9.92 PAAADD4059 pKa = 3.27 ASNAVTVSGSASPAVSSATYY4079 pKa = 9.62 NAEE4082 pKa = 3.91 TGVITVTGSNFVKK4095 pKa = 10.71 QPGAANDD4102 pKa = 3.22 IDD4104 pKa = 4.0 VTKK4107 pKa = 9.69 FTFTGQGGSVTLSTTSKK4124 pKa = 10.7 VEE4126 pKa = 4.01 TSSGSTFSVTLSTADD4141 pKa = 3.4 KK4142 pKa = 10.87 ALVNALLDD4150 pKa = 3.97 KK4151 pKa = 11.31 NGTASSGGTAYY4162 pKa = 10.86 NLSLADD4168 pKa = 3.83 DD4169 pKa = 3.92 WNGPVTGGNTADD4181 pKa = 3.5 TTNPITVSGVKK4192 pKa = 9.99 VVEE4195 pKa = 4.42 TVDD4198 pKa = 3.65 GVAVTTEE4205 pKa = 4.32 TTTVTQTYY4213 pKa = 9.68 TDD4215 pKa = 4.0 LNGNTVTRR4223 pKa = 11.84 EE4224 pKa = 4.04 VEE4226 pKa = 4.28 TEE4228 pKa = 4.28 TVTVAPVTDD4237 pKa = 3.23 TRR4239 pKa = 11.84 VNQEE4243 pKa = 3.76 GPAATAEE4250 pKa = 4.25 IPLFWGEE4257 pKa = 3.98 SSRR4260 pKa = 11.84 TEE4262 pKa = 3.84 WATVASLPVGVGIATSGVRR4281 pKa = 11.84 SPVDD4285 pKa = 3.18 NRR4287 pKa = 11.84 TLNDD4291 pKa = 4.28 AIGDD4295 pKa = 3.81 LLYY4298 pKa = 11.2 YY4299 pKa = 9.98 IDD4301 pKa = 3.89 TTAPTTDD4308 pKa = 3.42 GARR4311 pKa = 11.84 TGMLAGGSGFLQTLSDD4327 pKa = 3.84 STDD4330 pKa = 3.07 TLIVNKK4336 pKa = 8.98 VTLTVAGTDD4345 pKa = 3.58 VPATPVVISGTANSVTTPAGTATPKK4370 pKa = 10.11 EE4371 pKa = 4.4 AIVIDD4376 pKa = 4.75 ASALPAGTHH4385 pKa = 6.99 LDD4387 pKa = 3.51 LQNIEE4392 pKa = 4.01 FAVIIGSGITVRR4404 pKa = 11.84 GGDD4407 pKa = 3.4 GANIVFAGEE4416 pKa = 4.45 GSQNILLGADD4426 pKa = 3.96 DD4427 pKa = 5.52 DD4428 pKa = 4.44 EE4429 pKa = 4.79 LHH4431 pKa = 7.11 AGDD4434 pKa = 4.86 GDD4436 pKa = 4.36 DD4437 pKa = 5.25 FVGSLAGNDD4446 pKa = 3.75 RR4447 pKa = 11.84 IFGEE4451 pKa = 4.53 GGNDD4455 pKa = 2.94 RR4456 pKa = 11.84 MSGGDD4461 pKa = 3.58 GNDD4464 pKa = 3.26 FMHH4467 pKa = 7.01 GGDD4470 pKa = 3.74 GMDD4473 pKa = 3.12 TVVYY4477 pKa = 9.43 SGNMKK4482 pKa = 10.53 DD4483 pKa = 3.85 YY4484 pKa = 10.66 IITRR4488 pKa = 11.84 DD4489 pKa = 3.18 HH4490 pKa = 6.62 GLTYY4494 pKa = 10.36 ISPVSNPSEE4503 pKa = 4.11 KK4504 pKa = 10.59 DD4505 pKa = 3.31 VILNSEE4511 pKa = 4.59 HH4512 pKa = 7.97 IEE4514 pKa = 4.26 FADD4517 pKa = 3.62 GTYY4520 pKa = 10.81 NIDD4523 pKa = 3.51 NSDD4526 pKa = 3.68 EE4527 pKa = 4.11 LTYY4530 pKa = 10.88 VASLYY4535 pKa = 8.68 TQILGRR4541 pKa = 11.84 QPEE4544 pKa = 4.3 VDD4546 pKa = 3.49 GFQYY4550 pKa = 10.33 WADD4553 pKa = 3.38 LVEE4556 pKa = 4.15 DD4557 pKa = 4.43 HH4558 pKa = 6.62 NLSVGDD4564 pKa = 3.53 MTIWFMKK4571 pKa = 8.59 SAEE4574 pKa = 4.16 YY4575 pKa = 8.3 EE4576 pKa = 3.99 TAAGVDD4582 pKa = 4.25 FDD4584 pKa = 4.67 TLSAADD4590 pKa = 3.84 QVEE4593 pKa = 4.14 QLYY4596 pKa = 10.08 VALLGRR4602 pKa = 11.84 PSDD4605 pKa = 4.32 ADD4607 pKa = 3.41 GKK4609 pKa = 10.8 AYY4611 pKa = 9.09 WLSVLDD4617 pKa = 4.11 SGHH4620 pKa = 6.84 TIQDD4624 pKa = 3.4 VAEE4627 pKa = 4.42 GFVTSVEE4634 pKa = 4.17 MQGVYY4639 pKa = 10.7 NLPDD4643 pKa = 3.32 DD4644 pKa = 4.05 WNFFSS4649 pKa = 5.2
Molecular weight: 477.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.528
IPC_protein 3.605
Toseland 3.35
ProMoST 3.77
Dawson 3.605
Bjellqvist 3.745
Wikipedia 3.541
Rodwell 3.414
Grimsley 3.261
Solomon 3.605
Lehninger 3.554
Nozaki 3.706
DTASelect 3.986
Thurlkill 3.414
EMBOSS 3.554
Sillero 3.719
Patrickios 0.947
IPC_peptide 3.592
IPC2_peptide 3.694
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|A0A4R1KBY2|A0A4R1KBY2_9BACT Soluble lytic murein transglycosylase OS=Seleniivibrio woodruffii OX=1078050 GN=C8D98_1507 PE=4 SV=1
MM1 pKa = 7.36 AQLTMRR7 pKa = 11.84 KK8 pKa = 9.1 PSNLKK13 pKa = 9.94 RR14 pKa = 11.84 KK15 pKa = 8.99 RR16 pKa = 11.84 AQGFRR21 pKa = 11.84 ARR23 pKa = 11.84 MKK25 pKa = 9.51 TKK27 pKa = 10.28 GGRR30 pKa = 11.84 AVIARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.8 GRR41 pKa = 11.84 KK42 pKa = 8.66 RR43 pKa = 11.84 LAVV46 pKa = 3.41
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2684
0
2684
883181
33
4649
329.1
36.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.164 ± 0.069
1.218 ± 0.022
5.767 ± 0.037
6.74 ± 0.051
4.71 ± 0.038
7.157 ± 0.054
1.763 ± 0.022
7.234 ± 0.042
6.95 ± 0.055
8.872 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.051 ± 0.03
4.307 ± 0.031
3.432 ± 0.028
2.667 ± 0.026
4.55 ± 0.037
6.418 ± 0.046
5.469 ± 0.058
7.178 ± 0.037
0.73 ± 0.014
3.621 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here