Streptococcus satellite phage Javan259

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZM15|A0A4D5ZM15_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan259 OX=2558595 GN=JavanS259_0008 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.26IKK4 pKa = 10.31IFYY7 pKa = 9.82QRR9 pKa = 11.84GSLTEE14 pKa = 4.12FEE16 pKa = 4.61EE17 pKa = 4.81EE18 pKa = 4.3INNFMATANVVDD30 pKa = 4.14VKK32 pKa = 10.43LTEE35 pKa = 4.13GTYY38 pKa = 11.19GDD40 pKa = 4.74YY41 pKa = 10.01EE42 pKa = 4.6TINCSLTAVIVYY54 pKa = 9.99RR55 pKa = 11.84EE56 pKa = 4.04INGVFNPVPTYY67 pKa = 11.07HH68 pKa = 6.45NN69 pKa = 3.66

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZH27|A0A4D5ZH27_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan259 OX=2558595 GN=JavanS259_0011 PE=4 SV=1
MM1 pKa = 7.8TITEE5 pKa = 4.2YY6 pKa = 10.79KK7 pKa = 9.72KK8 pKa = 10.89KK9 pKa = 10.51NGEE12 pKa = 3.66LVYY15 pKa = 10.62RR16 pKa = 11.84SNIYY20 pKa = 10.54LGIDD24 pKa = 2.8RR25 pKa = 11.84VTGKK29 pKa = 10.3KK30 pKa = 10.03VRR32 pKa = 11.84TTITAPTKK40 pKa = 10.63KK41 pKa = 10.26LLKK44 pKa = 9.43TKK46 pKa = 10.2ARR48 pKa = 11.84QAIADD53 pKa = 4.17FEE55 pKa = 5.68ANGHH59 pKa = 5.08TIKK62 pKa = 10.68PKK64 pKa = 10.69LVVKK68 pKa = 9.48TYY70 pKa = 11.48GEE72 pKa = 4.32LAQLWWDD79 pKa = 3.92SYY81 pKa = 11.07KK82 pKa = 9.87HH83 pKa = 4.6TVKK86 pKa = 10.72PNTSCMMEE94 pKa = 4.09SFVRR98 pKa = 11.84NHH100 pKa = 6.37LLSVFADD107 pKa = 3.8YY108 pKa = 10.8PLDD111 pKa = 3.91KK112 pKa = 9.04MTTPMIQMQVNEE124 pKa = 4.36WASKK128 pKa = 9.4ANKK131 pKa = 9.35GATGAFQRR139 pKa = 11.84YY140 pKa = 8.73GLLCSLNRR148 pKa = 11.84RR149 pKa = 11.84ILQYY153 pKa = 11.17GVTLEE158 pKa = 4.36LLSFNPAKK166 pKa = 10.85NIVLPRR172 pKa = 11.84KK173 pKa = 7.84TRR175 pKa = 11.84VKK177 pKa = 10.63AKK179 pKa = 10.73DD180 pKa = 3.58KK181 pKa = 10.73IKK183 pKa = 10.96HH184 pKa = 6.26LDD186 pKa = 3.4QDD188 pKa = 3.76NLKK191 pKa = 10.99AFLTYY196 pKa = 10.62LSRR199 pKa = 11.84LEE201 pKa = 3.78PTYY204 pKa = 11.11QHH206 pKa = 6.23MFEE209 pKa = 3.89IMLYY213 pKa = 8.55KK214 pKa = 10.18TLLATGCRR222 pKa = 11.84ISEE225 pKa = 4.2LLALEE230 pKa = 4.26WSDD233 pKa = 3.7IDD235 pKa = 4.13FDD237 pKa = 4.03EE238 pKa = 4.74AQISITKK245 pKa = 8.33TLNRR249 pKa = 11.84YY250 pKa = 7.38RR251 pKa = 11.84TVNSPKK257 pKa = 9.29SKK259 pKa = 10.5SSIRR263 pKa = 11.84VIDD266 pKa = 3.52IDD268 pKa = 3.91QATLKK273 pKa = 9.54MLKK276 pKa = 9.83SYY278 pKa = 10.34RR279 pKa = 11.84QQQRR283 pKa = 11.84LEE285 pKa = 4.05AGYY288 pKa = 9.45QPKK291 pKa = 10.07IVFSPITDD299 pKa = 4.04TYY301 pKa = 11.69AKK303 pKa = 10.12VASLRR308 pKa = 11.84QRR310 pKa = 11.84LEE312 pKa = 3.48RR313 pKa = 11.84HH314 pKa = 5.19FRR316 pKa = 11.84LAGVEE321 pKa = 3.86NVGFHH326 pKa = 6.51GFRR329 pKa = 11.84HH330 pKa = 4.86THH332 pKa = 6.74ASLLLNAGIPYY343 pKa = 10.01KK344 pKa = 10.28EE345 pKa = 3.98LQHH348 pKa = 7.05RR349 pKa = 11.84LGHH352 pKa = 5.92NNISTTMDD360 pKa = 2.78IYY362 pKa = 11.63GHH364 pKa = 6.4LSEE367 pKa = 4.86MNKK370 pKa = 9.94KK371 pKa = 9.68SAVSFFEE378 pKa = 3.98NAINEE383 pKa = 4.37LKK385 pKa = 10.82VV386 pKa = 3.17

Molecular weight: 44.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3482

49

535

158.3

18.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.83 ± 0.326

0.574 ± 0.173

6.318 ± 0.397

8.702 ± 0.727

4.423 ± 0.508

4.968 ± 0.408

1.895 ± 0.261

6.72 ± 0.309

8.845 ± 0.467

9.075 ± 0.508

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.239

5.227 ± 0.338

2.298 ± 0.201

4.193 ± 0.248

4.94 ± 0.46

5.313 ± 0.39

6.519 ± 0.391

5.773 ± 0.34

1.149 ± 0.228

4.681 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski