Amino acid dipepetide frequency for Galleria mellonella (Greater wax moth)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.592AlaAla: 7.592 ± 0.065	1.314AlaCys: 1.314 ± 0.022	3.358AlaAsp: 3.358 ± 0.02	4.283AlaGlu: 4.283 ± 0.033	2.162AlaPhe: 2.162 ± 0.018	4.259AlaGly: 4.259 ± 0.036	1.73AlaHis: 1.73 ± 0.016	3.251AlaIle: 3.251 ± 0.023	3.572AlaLys: 3.572 ± 0.028	6.629AlaLeu: 6.629 ± 0.048	1.456AlaMet: 1.456 ± 0.014	2.619AlaAsn: 2.619 ± 0.017	4.101AlaPro: 4.101 ± 0.038	2.555AlaGln: 2.555 ± 0.025	4.045AlaArg: 4.045 ± 0.032	5.041AlaSer: 5.041 ± 0.032	3.917AlaThr: 3.917 ± 0.025	4.485AlaVal: 4.485 ± 0.024	0.67AlaTrp: 0.67 ± 0.01	1.769AlaTyr: 1.769 ± 0.017	0.002AlaXaa: 0.002 ± 0.001
Cys	1.307CysAla: 1.307 ± 0.016	0.468CysCys: 0.468 ± 0.008	1.169CysAsp: 1.169 ± 0.016	1.168CysGlu: 1.168 ± 0.017	0.692CysPhe: 0.692 ± 0.011	1.38CysGly: 1.38 ± 0.028	0.463CysHis: 0.463 ± 0.008	1.024CysIle: 1.024 ± 0.023	1.108CysLys: 1.108 ± 0.017	1.707CysLeu: 1.707 ± 0.022	0.379CysMet: 0.379 ± 0.007	0.957CysAsn: 0.957 ± 0.015	0.995CysPro: 0.995 ± 0.023	0.685CysGln: 0.685 ± 0.014	1.127CysArg: 1.127 ± 0.024	1.53CysSer: 1.53 ± 0.023	1.116CysThr: 1.116 ± 0.022	1.294CysVal: 1.294 ± 0.019	0.233CysTrp: 0.233 ± 0.005	0.604CysTyr: 0.604 ± 0.009	0.001CysXaa: 0.001 ± 0.0
Asp	3.361AspAla: 3.361 ± 0.021	0.973AspCys: 0.973 ± 0.016	3.929AspAsp: 3.929 ± 0.029	4.25AspGlu: 4.25 ± 0.028	1.995AspPhe: 1.995 ± 0.015	3.215AspGly: 3.215 ± 0.032	1.166AspHis: 1.166 ± 0.01	3.537AspIle: 3.537 ± 0.023	3.624AspLys: 3.624 ± 0.027	4.735AspLeu: 4.735 ± 0.03	1.305AspMet: 1.305 ± 0.013	2.857AspAsn: 2.857 ± 0.02	2.537AspPro: 2.537 ± 0.026	1.631AspGln: 1.631 ± 0.015	2.77AspArg: 2.77 ± 0.025	4.254AspSer: 4.254 ± 0.029	3.1AspThr: 3.1 ± 0.018	3.672AspVal: 3.672 ± 0.022	0.599AspTrp: 0.599 ± 0.01	1.861AspTyr: 1.861 ± 0.015	0.001AspXaa: 0.001 ± 0.0
Glu	4.378GluAla: 4.378 ± 0.036	1.228GluCys: 1.228 ± 0.03	3.992GluAsp: 3.992 ± 0.026	6.078GluGlu: 6.078 ± 0.05	2.063GluPhe: 2.063 ± 0.018	3.223GluGly: 3.223 ± 0.032	1.523GluHis: 1.523 ± 0.016	3.903GluIle: 3.903 ± 0.029	5.175GluLys: 5.175 ± 0.047	5.945GluLeu: 5.945 ± 0.042	1.58GluMet: 1.58 ± 0.016	3.717GluAsn: 3.717 ± 0.026	2.991GluPro: 2.991 ± 0.037	2.74GluGln: 2.74 ± 0.03	4.079GluArg: 4.079 ± 0.03	4.614GluSer: 4.614 ± 0.03	3.669GluThr: 3.669 ± 0.029	3.922GluVal: 3.922 ± 0.023	0.668GluTrp: 0.668 ± 0.01	2.013GluTyr: 2.013 ± 0.016	0.001GluXaa: 0.001 ± 0.0
Phe	2.035PheAla: 2.035 ± 0.017	0.727PheCys: 0.727 ± 0.01	2.011PheAsp: 2.011 ± 0.014	2.0PheGlu: 2.0 ± 0.017	1.444PhePhe: 1.444 ± 0.017	2.143PheGly: 2.143 ± 0.018	0.886PheHis: 0.886 ± 0.011	2.085PheIle: 2.085 ± 0.019	2.069PheLys: 2.069 ± 0.015	3.185PheLeu: 3.185 ± 0.026	0.79PheMet: 0.79 ± 0.011	1.77PheAsn: 1.77 ± 0.016	1.488PhePro: 1.488 ± 0.014	1.266PheGln: 1.266 ± 0.011	1.834PheArg: 1.834 ± 0.018	2.659PheSer: 2.659 ± 0.019	2.096PheThr: 2.096 ± 0.018	2.324PheVal: 2.324 ± 0.017	0.394PheTrp: 0.394 ± 0.007	1.296PheTyr: 1.296 ± 0.013	0.002PheXaa: 0.002 ± 0.0
Gly	4.227GlyAla: 4.227 ± 0.033	0.995GlyCys: 0.995 ± 0.015	3.045GlyAsp: 3.045 ± 0.026	3.468GlyGlu: 3.468 ± 0.036	2.046GlyPhe: 2.046 ± 0.017	4.827GlyGly: 4.827 ± 0.052	1.313GlyHis: 1.313 ± 0.017	2.684GlyIle: 2.684 ± 0.022	3.213GlyLys: 3.213 ± 0.028	4.468GlyLeu: 4.468 ± 0.026	1.099GlyMet: 1.099 ± 0.014	2.39GlyAsn: 2.39 ± 0.023	2.617GlyPro: 2.617 ± 0.045	2.007GlyGln: 2.007 ± 0.02	3.164GlyArg: 3.164 ± 0.024	4.525GlySer: 4.525 ± 0.037	3.014GlyThr: 3.014 ± 0.023	3.644GlyVal: 3.644 ± 0.026	0.719GlyTrp: 0.719 ± 0.01	2.029GlyTyr: 2.029 ± 0.022	0.003GlyXaa: 0.003 ± 0.001
His	1.638HisAla: 1.638 ± 0.017	0.548HisCys: 0.548 ± 0.008	1.2HisAsp: 1.2 ± 0.012	1.404HisGlu: 1.404 ± 0.013	0.884HisPhe: 0.884 ± 0.011	1.304HisGly: 1.304 ± 0.016	1.025HisHis: 1.025 ± 0.021	1.412HisIle: 1.412 ± 0.014	1.45HisLys: 1.45 ± 0.015	2.302HisLeu: 2.302 ± 0.02	0.691HisMet: 0.691 ± 0.011	1.201HisAsn: 1.201 ± 0.014	1.338HisPro: 1.338 ± 0.015	1.022HisGln: 1.022 ± 0.012	1.426HisArg: 1.426 ± 0.015	1.979HisSer: 1.979 ± 0.019	1.527HisThr: 1.527 ± 0.015	1.48HisVal: 1.48 ± 0.012	0.297HisTrp: 0.297 ± 0.007	0.94HisTyr: 0.94 ± 0.011	0.001HisXaa: 0.001 ± 0.0
Ile	3.371IleAla: 3.371 ± 0.02	1.177IleCys: 1.177 ± 0.02	3.169IleAsp: 3.169 ± 0.024	3.722IleGlu: 3.722 ± 0.033	2.088IlePhe: 2.088 ± 0.02	2.661IleGly: 2.661 ± 0.02	1.284IleHis: 1.284 ± 0.014	3.415IleIle: 3.415 ± 0.027	3.793IleLys: 3.793 ± 0.027	4.796IleLeu: 4.796 ± 0.034	1.161IleMet: 1.161 ± 0.013	2.991IleAsn: 2.991 ± 0.024	2.714IlePro: 2.714 ± 0.02	2.172IleGln: 2.172 ± 0.018	2.611IleArg: 2.611 ± 0.017	4.216IleSer: 4.216 ± 0.027	3.433IleThr: 3.433 ± 0.027	3.511IleVal: 3.511 ± 0.023	0.506IleTrp: 0.506 ± 0.007	1.719IleTyr: 1.719 ± 0.017	0.002IleXaa: 0.002 ± 0.001
Lys	3.516LysAla: 3.516 ± 0.028	1.22LysCys: 1.22 ± 0.019	3.557LysAsp: 3.557 ± 0.031	4.913LysGlu: 4.913 ± 0.04	2.071LysPhe: 2.071 ± 0.018	2.681LysGly: 2.681 ± 0.025	1.54LysHis: 1.54 ± 0.016	3.764LysIle: 3.764 ± 0.03	5.583LysLys: 5.583 ± 0.042	5.735LysLeu: 5.735 ± 0.039	1.534LysMet: 1.534 ± 0.015	3.441LysAsn: 3.441 ± 0.027	3.43LysPro: 3.43 ± 0.06	2.629LysGln: 2.629 ± 0.022	3.699LysArg: 3.699 ± 0.023	4.835LysSer: 4.835 ± 0.04	3.627LysThr: 3.627 ± 0.025	3.636LysVal: 3.636 ± 0.026	0.666LysTrp: 0.666 ± 0.013	2.281LysTyr: 2.281 ± 0.021	0.003LysXaa: 0.003 ± 0.001
Leu	6.397LeuAla: 6.397 ± 0.039	1.727LeuCys: 1.727 ± 0.018	4.762LeuAsp: 4.762 ± 0.032	6.123LeuGlu: 6.123 ± 0.047	3.038LeuPhe: 3.038 ± 0.027	4.336LeuGly: 4.336 ± 0.028	2.394LeuHis: 2.394 ± 0.021	4.311LeuIle: 4.311 ± 0.03	5.978LeuLys: 5.978 ± 0.035	8.728LeuLeu: 8.728 ± 0.06	1.899LeuMet: 1.899 ± 0.016	4.318LeuAsn: 4.318 ± 0.028	4.758LeuPro: 4.758 ± 0.029	4.43LeuGln: 4.43 ± 0.036	5.35LeuArg: 5.35 ± 0.037	6.873LeuSer: 6.873 ± 0.035	4.953LeuThr: 4.953 ± 0.025	5.205LeuVal: 5.205 ± 0.029	0.884LeuTrp: 0.884 ± 0.011	2.708LeuTyr: 2.708 ± 0.022	0.002LeuXaa: 0.002 ± 0.001
Met	1.589MetAla: 1.589 ± 0.014	0.433MetCys: 0.433 ± 0.007	1.243MetAsp: 1.243 ± 0.012	1.586MetGlu: 1.586 ± 0.016	0.89MetPhe: 0.89 ± 0.012	1.146MetGly: 1.146 ± 0.017	0.527MetHis: 0.527 ± 0.008	0.993MetIle: 0.993 ± 0.011	1.415MetLys: 1.415 ± 0.013	1.998MetLeu: 1.998 ± 0.019	0.612MetMet: 0.612 ± 0.011	1.048MetAsn: 1.048 ± 0.012	1.087MetPro: 1.087 ± 0.012	0.958MetGln: 0.958 ± 0.012	1.196MetArg: 1.196 ± 0.013	1.815MetSer: 1.815 ± 0.016	1.207MetThr: 1.207 ± 0.011	1.26MetVal: 1.26 ± 0.011	0.269MetTrp: 0.269 ± 0.006	0.72MetTyr: 0.72 ± 0.011	0.001MetXaa: 0.001 ± 0.0
Asn	2.682AsnAla: 2.682 ± 0.024	0.916AsnCys: 0.916 ± 0.012	2.69AsnAsp: 2.69 ± 0.02	3.341AsnGlu: 3.341 ± 0.024	1.833AsnPhe: 1.833 ± 0.016	2.823AsnGly: 2.823 ± 0.025	1.07AsnHis: 1.07 ± 0.015	3.616AsnIle: 3.616 ± 0.028	3.629AsnLys: 3.629 ± 0.028	4.222AsnLeu: 4.222 ± 0.031	1.253AsnMet: 1.253 ± 0.013	3.366AsnAsn: 3.366 ± 0.031	2.158AsnPro: 2.158 ± 0.024	1.818AsnGln: 1.818 ± 0.016	2.233AsnArg: 2.233 ± 0.017	3.908AsnSer: 3.908 ± 0.036	2.939AsnThr: 2.939 ± 0.022	3.249AsnVal: 3.249 ± 0.022	0.479AsnTrp: 0.479 ± 0.008	1.71AsnTyr: 1.71 ± 0.018	0.002AsnXaa: 0.002 ± 0.001
Pro	4.18ProAla: 4.18 ± 0.035	0.82ProCys: 0.82 ± 0.033	2.883ProAsp: 2.883 ± 0.022	3.573ProGlu: 3.573 ± 0.033	1.597ProPhe: 1.597 ± 0.017	2.995ProGly: 2.995 ± 0.051	1.532ProHis: 1.532 ± 0.019	2.601ProIle: 2.601 ± 0.023	3.042ProLys: 3.042 ± 0.031	4.455ProLeu: 4.455 ± 0.03	0.95ProMet: 0.95 ± 0.013	2.308ProAsn: 2.308 ± 0.019	5.366ProPro: 5.366 ± 0.06	2.29ProGln: 2.29 ± 0.025	2.981ProArg: 2.981 ± 0.026	4.384ProSer: 4.384 ± 0.037	3.292ProThr: 3.292 ± 0.033	3.512ProVal: 3.512 ± 0.027	0.475ProTrp: 0.475 ± 0.007	1.65ProTyr: 1.65 ± 0.02	0.001ProXaa: 0.001 ± 0.0
Gln	2.623GlnAla: 2.623 ± 0.026	0.772GlnCys: 0.772 ± 0.015	1.856GlnAsp: 1.856 ± 0.015	2.705GlnGlu: 2.705 ± 0.023	1.31GlnPhe: 1.31 ± 0.013	1.817GlnGly: 1.817 ± 0.021	1.207GlnHis: 1.207 ± 0.014	2.171GlnIle: 2.171 ± 0.018	2.461GlnLys: 2.461 ± 0.022	3.897GlnLeu: 3.897 ± 0.034	0.99GlnMet: 0.99 ± 0.013	2.172GlnAsn: 2.172 ± 0.02	2.305GlnPro: 2.305 ± 0.024	2.745GlnGln: 2.745 ± 0.057	2.45GlnArg: 2.45 ± 0.021	2.889GlnSer: 2.889 ± 0.025	2.167GlnThr: 2.167 ± 0.017	2.215GlnVal: 2.215 ± 0.016	0.461GlnTrp: 0.461 ± 0.007	1.398GlnTyr: 1.398 ± 0.014	0.001GlnXaa: 0.001 ± 0.0
Arg	4.123ArgAla: 4.123 ± 0.029	1.118ArgCys: 1.118 ± 0.016	3.1ArgAsp: 3.1 ± 0.027	3.515ArgGlu: 3.515 ± 0.025	1.798ArgPhe: 1.798 ± 0.013	3.014ArgGly: 3.014 ± 0.026	1.65ArgHis: 1.65 ± 0.016	2.74ArgIle: 2.74 ± 0.018	3.631ArgLys: 3.631 ± 0.026	5.071ArgLeu: 5.071 ± 0.032	1.082ArgMet: 1.082 ± 0.011	2.599ArgAsn: 2.599 ± 0.021	3.101ArgPro: 3.101 ± 0.031	2.262ArgGln: 2.262 ± 0.017	4.688ArgArg: 4.688 ± 0.041	4.356ArgSer: 4.356 ± 0.038	2.881ArgThr: 2.881 ± 0.017	3.224ArgVal: 3.224 ± 0.027	0.643ArgTrp: 0.643 ± 0.01	1.741ArgTyr: 1.741 ± 0.016	0.003ArgXaa: 0.003 ± 0.001
Ser	5.01SerAla: 5.01 ± 0.029	1.419SerCys: 1.419 ± 0.024	4.56SerAsp: 4.56 ± 0.028	4.983SerGlu: 4.983 ± 0.031	2.563SerPhe: 2.563 ± 0.018	4.73SerGly: 4.73 ± 0.038	1.772SerHis: 1.772 ± 0.018	3.906SerIle: 3.906 ± 0.028	4.672SerLys: 4.672 ± 0.035	6.721SerLeu: 6.721 ± 0.042	1.587SerMet: 1.587 ± 0.015	4.009SerAsn: 4.009 ± 0.038	4.867SerPro: 4.867 ± 0.045	3.122SerGln: 3.122 ± 0.026	4.192SerArg: 4.192 ± 0.033	7.967SerSer: 7.967 ± 0.083	4.918SerThr: 4.918 ± 0.034	4.694SerVal: 4.694 ± 0.025	0.818SerTrp: 0.818 ± 0.011	2.165SerTyr: 2.165 ± 0.019	0.002SerXaa: 0.002 ± 0.0
Thr	3.907ThrAla: 3.907 ± 0.023	1.158ThrCys: 1.158 ± 0.018	3.043ThrAsp: 3.043 ± 0.022	3.797ThrGlu: 3.797 ± 0.03	2.05ThrPhe: 2.05 ± 0.015	3.297ThrGly: 3.297 ± 0.03	1.423ThrHis: 1.423 ± 0.013	3.276ThrIle: 3.276 ± 0.022	3.457ThrLys: 3.457 ± 0.029	5.143ThrLeu: 5.143 ± 0.025	1.192ThrMet: 1.192 ± 0.011	2.84ThrAsn: 2.84 ± 0.019	3.803ThrPro: 3.803 ± 0.031	2.168ThrGln: 2.168 ± 0.017	2.65ThrArg: 2.65 ± 0.019	4.844ThrSer: 4.844 ± 0.031	4.099ThrThr: 4.099 ± 0.058	3.974ThrVal: 3.974 ± 0.025	0.609ThrTrp: 0.609 ± 0.01	1.733ThrTyr: 1.733 ± 0.017	0.001ThrXaa: 0.001 ± 0.0
Val	4.387ValAla: 4.387 ± 0.026	1.42ValCys: 1.42 ± 0.021	3.354ValAsp: 3.354 ± 0.022	3.953ValGlu: 3.953 ± 0.028	2.225ValPhe: 2.225 ± 0.019	3.165ValGly: 3.165 ± 0.022	1.476ValHis: 1.476 ± 0.014	3.465ValIle: 3.465 ± 0.025	3.84ValLys: 3.84 ± 0.032	5.535ValLeu: 5.535 ± 0.031	1.401ValMet: 1.401 ± 0.013	3.05ValAsn: 3.05 ± 0.022	3.389ValPro: 3.389 ± 0.03	2.413ValGln: 2.413 ± 0.016	3.298ValArg: 3.298 ± 0.021	4.751ValSer: 4.751 ± 0.025	4.081ValThr: 4.081 ± 0.027	4.199ValVal: 4.199 ± 0.031	0.693ValTrp: 0.693 ± 0.01	1.939ValTyr: 1.939 ± 0.017	0.003ValXaa: 0.003 ± 0.001
Trp	0.669TrpAla: 0.669 ± 0.01	0.24TrpCys: 0.24 ± 0.005	0.609TrpAsp: 0.609 ± 0.01	0.621TrpGlu: 0.621 ± 0.009	0.409TrpPhe: 0.409 ± 0.007	0.545TrpGly: 0.545 ± 0.009	0.244TrpHis: 0.244 ± 0.005	0.532TrpIle: 0.532 ± 0.009	0.658TrpLys: 0.658 ± 0.01	1.081TrpLeu: 1.081 ± 0.013	0.276TrpMet: 0.276 ± 0.005	0.512TrpAsn: 0.512 ± 0.009	0.445TrpPro: 0.445 ± 0.008	0.443TrpGln: 0.443 ± 0.008	0.78TrpArg: 0.78 ± 0.01	0.832TrpSer: 0.832 ± 0.012	0.577TrpThr: 0.577 ± 0.01	0.63TrpVal: 0.63 ± 0.01	0.208TrpTrp: 0.208 ± 0.005	0.379TrpTyr: 0.379 ± 0.007	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.815TyrAla: 1.815 ± 0.016	0.735TyrCys: 0.735 ± 0.011	1.842TyrAsp: 1.842 ± 0.018	2.018TyrGlu: 2.018 ± 0.016	1.345TyrPhe: 1.345 ± 0.014	1.935TyrGly: 1.935 ± 0.02	0.845TyrHis: 0.845 ± 0.01	1.821TyrIle: 1.821 ± 0.015	1.989TyrLys: 1.989 ± 0.018	2.819TyrLeu: 2.819 ± 0.023	0.755TyrMet: 0.755 ± 0.01	1.767TyrAsn: 1.767 ± 0.017	1.398TyrPro: 1.398 ± 0.019	1.254TyrGln: 1.254 ± 0.013	1.764TyrArg: 1.764 ± 0.017	2.402TyrSer: 2.402 ± 0.02	1.852TyrThr: 1.852 ± 0.014	1.903TyrVal: 1.903 ± 0.015	0.398TyrTrp: 0.398 ± 0.007	1.288TyrTyr: 1.288 ± 0.017	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.004XaaIle: 0.004 ± 0.001	0.003XaaLys: 0.003 ± 0.001	0.003XaaLeu: 0.003 ± 0.001	0.001XaaMet: 0.001 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.002XaaSer: 0.002 ± 0.0	0.002XaaThr: 0.002 ± 0.0	0.003XaaVal: 0.003 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.002XaaTyr: 0.002 ± 0.0	0.172XaaXaa: 0.172 ± 0.024

Statistics based on 18321 proteins (9854621 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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