Haloterrigena limicola JCM 13563
Average proteome isoelectric point is 4.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3519 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M0CNB2|M0CNB2_9EURY Uncharacterized protein OS=Haloterrigena limicola JCM 13563 OX=1230457 GN=C476_03693 PE=4 SV=1
MM1 pKa = 8.39 VLMEE5 pKa = 5.08 SDD7 pKa = 4.23 SEE9 pKa = 4.64 LEE11 pKa = 4.54 AGDD14 pKa = 3.68 TAPDD18 pKa = 3.8 FEE20 pKa = 5.72 LEE22 pKa = 4.19 GVDD25 pKa = 3.44 GNRR28 pKa = 11.84 YY29 pKa = 7.04 SHH31 pKa = 6.87 EE32 pKa = 4.18 SFVDD36 pKa = 3.61 NEE38 pKa = 3.93 ALLVVFTCNHH48 pKa = 5.85 CPYY51 pKa = 10.38 AQAKK55 pKa = 9.77 FDD57 pKa = 4.45 LLNDD61 pKa = 3.99 LSAEE65 pKa = 4.11 YY66 pKa = 10.88 DD67 pKa = 3.59 DD68 pKa = 4.23 VAVVGINSNDD78 pKa = 3.37 AEE80 pKa = 4.61 EE81 pKa = 4.79 YY82 pKa = 10.31 PDD84 pKa = 4.67 DD85 pKa = 4.5 SFEE88 pKa = 4.69 AMQTYY93 pKa = 10.47 VDD95 pKa = 5.34 DD96 pKa = 4.03 GTIDD100 pKa = 3.3 YY101 pKa = 9.96 DD102 pKa = 3.55 AYY104 pKa = 11.33 LRR106 pKa = 11.84 DD107 pKa = 3.58 EE108 pKa = 4.5 SQDD111 pKa = 3.2 VARR114 pKa = 11.84 AYY116 pKa = 10.5 GAVCTPDD123 pKa = 3.2 PFLFARR129 pKa = 11.84 SDD131 pKa = 3.68 GEE133 pKa = 3.95 FRR135 pKa = 11.84 LVYY138 pKa = 10.16 HH139 pKa = 6.68 GRR141 pKa = 11.84 LDD143 pKa = 4.38 DD144 pKa = 5.1 ALNPDD149 pKa = 4.2 DD150 pKa = 4.58 EE151 pKa = 4.55 PTRR154 pKa = 11.84 FHH156 pKa = 6.99 IRR158 pKa = 11.84 EE159 pKa = 4.45 AIDD162 pKa = 3.33 AVLAGEE168 pKa = 4.22 EE169 pKa = 4.79 VDD171 pKa = 6.13 LEE173 pKa = 4.31 WQPSQGCSIKK183 pKa = 10.42 WKK185 pKa = 10.59 DD186 pKa = 3.47 DD187 pKa = 3.27
Molecular weight: 21.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.516
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.859
Patrickios 1.189
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|M0CQN7|M0CQN7_9EURY PAS/PAC sensor protein OS=Haloterrigena limicola JCM 13563 OX=1230457 GN=C476_01670 PE=4 SV=1
MM1 pKa = 7.39 TNVNSRR7 pKa = 11.84 TANKK11 pKa = 10.16 LSIVSTLIDD20 pKa = 3.13 SALAFRR26 pKa = 11.84 RR27 pKa = 11.84 GRR29 pKa = 11.84 PKK31 pKa = 10.66 SGLLLLGAAALSSRR45 pKa = 11.84 IPGLGTAVSVLLRR58 pKa = 11.84 LVRR61 pKa = 11.84 RR62 pKa = 11.84 FRR64 pKa = 4.4
Molecular weight: 6.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 10.789
IPC_protein 12.384
Toseland 12.544
ProMoST 13.056
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.149
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.901
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3519
0
3519
958682
24
2167
272.4
29.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.69 ± 0.064
0.762 ± 0.015
8.556 ± 0.056
8.707 ± 0.062
3.214 ± 0.028
8.065 ± 0.04
2.04 ± 0.022
4.598 ± 0.031
1.895 ± 0.027
9.014 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.779 ± 0.019
2.383 ± 0.025
4.567 ± 0.027
2.698 ± 0.026
6.311 ± 0.048
5.512 ± 0.035
6.765 ± 0.036
8.624 ± 0.047
1.107 ± 0.016
2.71 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here