Globicatella sp. HMSC072A10
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2027 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E8YNY5|A0A1E8YNY5_9LACT Uncharacterized protein OS=Globicatella sp. HMSC072A10 OX=1739315 GN=HMPREF2811_05820 PE=4 SV=1
MM1 pKa = 7.74 AEE3 pKa = 3.91 ALIVFASLTGNTEE16 pKa = 3.52 EE17 pKa = 4.44 MADD20 pKa = 3.21 IVAEE24 pKa = 3.94 RR25 pKa = 11.84 LEE27 pKa = 3.93 EE28 pKa = 4.46 HH29 pKa = 7.41 DD30 pKa = 3.96 INVEE34 pKa = 4.44 IVDD37 pKa = 3.97 CMQADD42 pKa = 3.23 AHH44 pKa = 7.01 DD45 pKa = 4.16 FLDD48 pKa = 3.99 YY49 pKa = 10.49 DD50 pKa = 3.37 ICIIGSYY57 pKa = 9.51 TYY59 pKa = 10.89 GVDD62 pKa = 3.5 GVLPDD67 pKa = 4.37 EE68 pKa = 5.28 MIDD71 pKa = 3.47 FHH73 pKa = 8.86 KK74 pKa = 10.84 EE75 pKa = 3.51 LGEE78 pKa = 4.32 LDD80 pKa = 3.43 LTGKK84 pKa = 10.03 VFGVFGSGDD93 pKa = 3.31 DD94 pKa = 4.02 FYY96 pKa = 11.35 DD97 pKa = 3.85 YY98 pKa = 11.12 FCAAVDD104 pKa = 3.86 FFEE107 pKa = 4.48 NQFVATGATKK117 pKa = 10.29 GGEE120 pKa = 4.23 SVKK123 pKa = 10.75 VNLNAEE129 pKa = 4.37 DD130 pKa = 4.0 EE131 pKa = 4.87 DD132 pKa = 4.45 IEE134 pKa = 4.41 NLNALADD141 pKa = 5.1 AIASHH146 pKa = 6.89 FEE148 pKa = 4.06 SS149 pKa = 4.21
Molecular weight: 16.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.656
IPC_protein 3.656
Toseland 3.439
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.567
Rodwell 3.478
Grimsley 3.35
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.961
Thurlkill 3.49
EMBOSS 3.579
Sillero 3.77
Patrickios 0.693
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A1E8YTB4|A0A1E8YTB4_9LACT tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Globicatella sp. HMSC072A10 OX=1739315 GN=mnmG PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.36 KK9 pKa = 7.48 RR10 pKa = 11.84 TRR12 pKa = 11.84 KK13 pKa = 9.06 KK14 pKa = 8.41 VHH16 pKa = 5.85 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 9.36 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLRR32 pKa = 11.84 KK33 pKa = 9.13 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.68 GRR39 pKa = 11.84 AKK41 pKa = 10.7 LSAA44 pKa = 3.92
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.486
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2027
0
2027
657366
37
2283
324.3
36.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.934 ± 0.06
0.524 ± 0.013
5.473 ± 0.045
7.49 ± 0.066
4.446 ± 0.04
6.231 ± 0.049
2.018 ± 0.025
8.08 ± 0.058
6.222 ± 0.045
9.874 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.689 ± 0.023
5.135 ± 0.038
3.454 ± 0.024
4.424 ± 0.049
3.775 ± 0.042
5.757 ± 0.043
5.703 ± 0.044
6.8 ± 0.047
0.965 ± 0.02
4.005 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here