Bacillus sp. MRMR6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9Q097|A0A1Q9Q097_9BACI Histidine kinase OS=Bacillus sp. MRMR6 OX=1928617 GN=BTR25_11270 PE=4 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.92WFDD7 pKa = 4.01RR8 pKa = 11.84VCGEE12 pKa = 4.26LQDD15 pKa = 4.14HH16 pKa = 6.79LEE18 pKa = 4.62SICEE22 pKa = 4.08EE23 pKa = 4.21YY24 pKa = 11.03DD25 pKa = 3.18QVGQMSIEE33 pKa = 4.02RR34 pKa = 11.84AAKK37 pKa = 9.36HH38 pKa = 5.06PRR40 pKa = 11.84IEE42 pKa = 4.42FFVEE46 pKa = 3.82TADD49 pKa = 3.61MDD51 pKa = 4.24TEE53 pKa = 4.15EE54 pKa = 5.16LDD56 pKa = 3.33RR57 pKa = 11.84DD58 pKa = 4.02YY59 pKa = 11.43FCTVFFDD66 pKa = 3.96PQNEE70 pKa = 4.01EE71 pKa = 4.38FYY73 pKa = 10.64IDD75 pKa = 3.83TFDD78 pKa = 3.69VDD80 pKa = 3.62TGHH83 pKa = 6.24TAKK86 pKa = 10.51IILSDD91 pKa = 3.24IEE93 pKa = 4.36EE94 pKa = 4.85IIHH97 pKa = 5.75EE98 pKa = 4.38VHH100 pKa = 6.71EE101 pKa = 4.46SLHH104 pKa = 8.45DD105 pKa = 3.75YY106 pKa = 10.91MNDD109 pKa = 3.29DD110 pKa = 4.43DD111 pKa = 6.19HH112 pKa = 6.46YY113 pKa = 9.45TDD115 pKa = 4.69DD116 pKa = 4.16GVYY119 pKa = 8.92LTSEE123 pKa = 4.44DD124 pKa = 3.84DD125 pKa = 3.91DD126 pKa = 5.47VYY128 pKa = 11.8DD129 pKa = 4.32EE130 pKa = 5.41ADD132 pKa = 3.25PMMDD136 pKa = 3.57MVDD139 pKa = 4.2DD140 pKa = 4.27NDD142 pKa = 3.68GVEE145 pKa = 4.25YY146 pKa = 11.31YY147 pKa = 10.08EE148 pKa = 4.57VEE150 pKa = 4.29GVDD153 pKa = 3.23SGDD156 pKa = 3.05IFEE159 pKa = 5.44EE160 pKa = 4.69IDD162 pKa = 3.93VEE164 pKa = 4.22WSTPEE169 pKa = 3.5VTAFKK174 pKa = 10.83HH175 pKa = 5.1EE176 pKa = 4.6DD177 pKa = 3.76EE178 pKa = 5.29VEE180 pKa = 3.89VTYY183 pKa = 10.94QFGVVEE189 pKa = 4.12EE190 pKa = 4.47TGDD193 pKa = 3.58GVLRR197 pKa = 11.84RR198 pKa = 11.84VNRR201 pKa = 11.84IWTTDD206 pKa = 3.36DD207 pKa = 3.95EE208 pKa = 4.93LIKK211 pKa = 11.03DD212 pKa = 3.59EE213 pKa = 4.46SHH215 pKa = 7.38FIFSKK220 pKa = 10.93EE221 pKa = 3.83EE222 pKa = 3.63ASTIIAMIASHH233 pKa = 6.86MDD235 pKa = 3.18QLSDD239 pKa = 3.95FDD241 pKa = 4.64FDD243 pKa = 4.42QMPP246 pKa = 3.46

Molecular weight: 28.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9Q4J7|A0A1Q9Q4J7_9BACI 50S ribosomal protein L21 OS=Bacillus sp. MRMR6 OX=1928617 GN=rplU PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5021

0

5021

1505097

21

1979

299.8

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.789 ± 0.036

0.758 ± 0.012

4.939 ± 0.025

7.44 ± 0.037

4.679 ± 0.025

6.982 ± 0.032

2.06 ± 0.02

7.923 ± 0.035

6.89 ± 0.03

9.831 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.017

4.643 ± 0.028

3.659 ± 0.022

3.617 ± 0.026

3.973 ± 0.025

6.005 ± 0.024

5.454 ± 0.028

6.954 ± 0.031

1.08 ± 0.013

3.601 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski