Amino acid dipepetide frequency for Bacillus sp. MRMR6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.834AlaAla: 5.834 ± 0.088	0.601AlaCys: 0.601 ± 0.02	3.341AlaAsp: 3.341 ± 0.048	4.706AlaGlu: 4.706 ± 0.059	3.112AlaPhe: 3.112 ± 0.052	5.479AlaGly: 5.479 ± 0.078	1.198AlaHis: 1.198 ± 0.034	5.74AlaIle: 5.74 ± 0.076	4.526AlaLys: 4.526 ± 0.061	6.739AlaLeu: 6.739 ± 0.074	1.874AlaMet: 1.874 ± 0.037	2.895AlaAsn: 2.895 ± 0.039	2.03AlaPro: 2.03 ± 0.048	1.99AlaGln: 1.99 ± 0.035	2.525AlaArg: 2.525 ± 0.042	3.939AlaSer: 3.939 ± 0.06	3.266AlaThr: 3.266 ± 0.05	5.207AlaVal: 5.207 ± 0.064	0.66AlaTrp: 0.66 ± 0.023	2.228AlaTyr: 2.228 ± 0.041	0.003AlaXaa: 0.003 ± 0.002
Cys	0.435CysAla: 0.435 ± 0.017	0.094CysCys: 0.094 ± 0.009	0.393CysAsp: 0.393 ± 0.017	0.518CysGlu: 0.518 ± 0.02	0.336CysPhe: 0.336 ± 0.017	0.714CysGly: 0.714 ± 0.02	0.262CysHis: 0.262 ± 0.019	0.534CysIle: 0.534 ± 0.021	0.381CysLys: 0.381 ± 0.015	0.728CysLeu: 0.728 ± 0.023	0.191CysMet: 0.191 ± 0.012	0.324CysAsn: 0.324 ± 0.014	0.374CysPro: 0.374 ± 0.018	0.25CysGln: 0.25 ± 0.014	0.302CysArg: 0.302 ± 0.015	0.539CysSer: 0.539 ± 0.019	0.424CysThr: 0.424 ± 0.018	0.438CysVal: 0.438 ± 0.016	0.088CysTrp: 0.088 ± 0.008	0.257CysTyr: 0.257 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.024AspAla: 3.024 ± 0.05	0.381AspCys: 0.381 ± 0.016	2.262AspAsp: 2.262 ± 0.047	3.993AspGlu: 3.993 ± 0.054	2.476AspPhe: 2.476 ± 0.04	3.388AspGly: 3.388 ± 0.057	1.218AspHis: 1.218 ± 0.032	3.935AspIle: 3.935 ± 0.052	2.967AspLys: 2.967 ± 0.049	5.024AspLeu: 5.024 ± 0.057	1.251AspMet: 1.251 ± 0.028	1.813AspAsn: 1.813 ± 0.036	1.988AspPro: 1.988 ± 0.041	1.991AspGln: 1.991 ± 0.042	2.258AspArg: 2.258 ± 0.046	2.685AspSer: 2.685 ± 0.04	2.401AspThr: 2.401 ± 0.039	3.506AspVal: 3.506 ± 0.05	0.662AspTrp: 0.662 ± 0.02	2.161AspTyr: 2.161 ± 0.037	0.002AspXaa: 0.002 ± 0.001
Glu	5.066GluAla: 5.066 ± 0.07	0.42GluCys: 0.42 ± 0.018	3.561GluAsp: 3.561 ± 0.061	6.675GluGlu: 6.675 ± 0.091	2.814GluPhe: 2.814 ± 0.042	4.368GluGly: 4.368 ± 0.053	1.517GluHis: 1.517 ± 0.03	5.852GluIle: 5.852 ± 0.066	6.507GluLys: 6.507 ± 0.071	7.264GluLeu: 7.264 ± 0.083	2.309GluMet: 2.309 ± 0.037	3.824GluAsn: 3.824 ± 0.059	1.971GluPro: 1.971 ± 0.035	3.165GluGln: 3.165 ± 0.057	3.333GluArg: 3.333 ± 0.055	3.415GluSer: 3.415 ± 0.055	3.883GluThr: 3.883 ± 0.054	5.13GluVal: 5.13 ± 0.065	0.853GluTrp: 0.853 ± 0.024	2.476GluTyr: 2.476 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.018PheAla: 3.018 ± 0.05	0.377PheCys: 0.377 ± 0.016	2.387PheAsp: 2.387 ± 0.04	3.01PheGlu: 3.01 ± 0.041	2.472PhePhe: 2.472 ± 0.056	3.341PheGly: 3.341 ± 0.048	1.034PheHis: 1.034 ± 0.027	4.026PheIle: 4.026 ± 0.064	2.572PheLys: 2.572 ± 0.047	4.824PheLeu: 4.824 ± 0.074	1.181PheMet: 1.181 ± 0.025	2.103PheAsn: 2.103 ± 0.036	1.759PhePro: 1.759 ± 0.033	1.597PheGln: 1.597 ± 0.033	1.6PheArg: 1.6 ± 0.031	3.333PheSer: 3.333 ± 0.05	2.728PheThr: 2.728 ± 0.044	3.121PheVal: 3.121 ± 0.047	0.533PheTrp: 0.533 ± 0.019	1.773PheTyr: 1.773 ± 0.04	0.003PheXaa: 0.003 ± 0.001
Gly	4.882GlyAla: 4.882 ± 0.076	0.646GlyCys: 0.646 ± 0.021	3.129GlyAsp: 3.129 ± 0.054	4.624GlyGlu: 4.624 ± 0.071	3.498GlyPhe: 3.498 ± 0.053	4.921GlyGly: 4.921 ± 0.078	1.394GlyHis: 1.394 ± 0.033	6.032GlyIle: 6.032 ± 0.073	5.171GlyLys: 5.171 ± 0.062	6.649GlyLeu: 6.649 ± 0.076	2.124GlyMet: 2.124 ± 0.042	2.881GlyAsn: 2.881 ± 0.047	1.961GlyPro: 1.961 ± 0.072	2.244GlyGln: 2.244 ± 0.04	2.626GlyArg: 2.626 ± 0.039	4.101GlySer: 4.101 ± 0.055	4.157GlyThr: 4.157 ± 0.069	5.025GlyVal: 5.025 ± 0.059	0.876GlyTrp: 0.876 ± 0.025	2.878GlyTyr: 2.878 ± 0.051	0.006GlyXaa: 0.006 ± 0.002
His	1.223HisAla: 1.223 ± 0.031	0.216HisCys: 0.216 ± 0.014	1.0HisAsp: 1.0 ± 0.031	1.381HisGlu: 1.381 ± 0.034	1.073HisPhe: 1.073 ± 0.026	1.43HisGly: 1.43 ± 0.031	0.637HisHis: 0.637 ± 0.023	1.428HisIle: 1.428 ± 0.035	1.027HisLys: 1.027 ± 0.024	2.143HisLeu: 2.143 ± 0.036	0.484HisMet: 0.484 ± 0.017	0.813HisAsn: 0.813 ± 0.024	1.104HisPro: 1.104 ± 0.032	0.87HisGln: 0.87 ± 0.023	0.883HisArg: 0.883 ± 0.026	1.313HisSer: 1.313 ± 0.032	1.055HisThr: 1.055 ± 0.026	1.347HisVal: 1.347 ± 0.026	0.25HisTrp: 0.25 ± 0.013	0.924HisTyr: 0.924 ± 0.024	0.001HisXaa: 0.001 ± 0.001
Ile	5.719IleAla: 5.719 ± 0.078	0.668IleCys: 0.668 ± 0.023	4.311IleAsp: 4.311 ± 0.056	5.751IleGlu: 5.751 ± 0.057	3.526IlePhe: 3.526 ± 0.054	5.93IleGly: 5.93 ± 0.078	1.708IleHis: 1.708 ± 0.036	6.082IleIle: 6.082 ± 0.089	4.924IleLys: 4.924 ± 0.06	7.314IleLeu: 7.314 ± 0.088	1.801IleMet: 1.801 ± 0.037	3.682IleAsn: 3.682 ± 0.054	3.453IlePro: 3.453 ± 0.05	2.818IleGln: 2.818 ± 0.046	3.121IleArg: 3.121 ± 0.046	5.287IleSer: 5.287 ± 0.066	4.545IleThr: 4.545 ± 0.061	5.355IleVal: 5.355 ± 0.071	0.718IleTrp: 0.718 ± 0.019	2.514IleTyr: 2.514 ± 0.038	0.003IleXaa: 0.003 ± 0.001
Lys	4.537LysAla: 4.537 ± 0.059	0.355LysCys: 0.355 ± 0.015	3.691LysAsp: 3.691 ± 0.056	6.619LysGlu: 6.619 ± 0.078	2.143LysPhe: 2.143 ± 0.038	4.545LysGly: 4.545 ± 0.055	1.28LysHis: 1.28 ± 0.032	4.993LysIle: 4.993 ± 0.063	5.696LysLys: 5.696 ± 0.062	6.018LysLeu: 6.018 ± 0.066	2.113LysMet: 2.113 ± 0.037	3.598LysAsn: 3.598 ± 0.055	2.223LysPro: 2.223 ± 0.042	2.89LysGln: 2.89 ± 0.047	3.08LysArg: 3.08 ± 0.048	3.553LysSer: 3.553 ± 0.043	3.595LysThr: 3.595 ± 0.054	4.846LysVal: 4.846 ± 0.063	0.836LysTrp: 0.836 ± 0.024	2.291LysTyr: 2.291 ± 0.044	0.003LysXaa: 0.003 ± 0.001
Leu	7.133LeuAla: 7.133 ± 0.079	0.7LeuCys: 0.7 ± 0.02	4.662LeuAsp: 4.662 ± 0.056	6.949LeuGlu: 6.949 ± 0.074	4.91LeuPhe: 4.91 ± 0.072	6.478LeuGly: 6.478 ± 0.077	1.867LeuHis: 1.867 ± 0.038	7.367LeuIle: 7.367 ± 0.097	6.805LeuLys: 6.805 ± 0.073	10.237LeuLeu: 10.237 ± 0.119	2.557LeuMet: 2.557 ± 0.042	4.477LeuAsn: 4.477 ± 0.051	3.912LeuPro: 3.912 ± 0.054	3.503LeuGln: 3.503 ± 0.051	3.689LeuArg: 3.689 ± 0.055	6.649LeuSer: 6.649 ± 0.07	5.809LeuThr: 5.809 ± 0.067	6.497LeuVal: 6.497 ± 0.079	0.846LeuTrp: 0.846 ± 0.025	3.254LeuTyr: 3.254 ± 0.044	0.009LeuXaa: 0.009 ± 0.002
Met	1.981MetAla: 1.981 ± 0.036	0.151MetCys: 0.151 ± 0.01	1.39MetAsp: 1.39 ± 0.032	1.945MetGlu: 1.945 ± 0.036	1.104MetPhe: 1.104 ± 0.031	1.861MetGly: 1.861 ± 0.055	0.423MetHis: 0.423 ± 0.016	2.166MetIle: 2.166 ± 0.044	2.393MetLys: 2.393 ± 0.039	2.532MetLeu: 2.532 ± 0.043	0.825MetMet: 0.825 ± 0.023	1.528MetAsn: 1.528 ± 0.036	1.013MetPro: 1.013 ± 0.029	0.808MetGln: 0.808 ± 0.023	1.024MetArg: 1.024 ± 0.029	1.597MetSer: 1.597 ± 0.031	1.512MetThr: 1.512 ± 0.029	1.947MetVal: 1.947 ± 0.044	0.206MetTrp: 0.206 ± 0.012	0.731MetTyr: 0.731 ± 0.023	0.001MetXaa: 0.001 ± 0.001
Asn	2.814AsnAla: 2.814 ± 0.04	0.38AsnCys: 0.38 ± 0.019	2.244AsnAsp: 2.244 ± 0.041	3.545AsnGlu: 3.545 ± 0.057	1.768AsnPhe: 1.768 ± 0.034	3.594AsnGly: 3.594 ± 0.06	1.122AsnHis: 1.122 ± 0.027	3.501AsnIle: 3.501 ± 0.057	3.04AsnLys: 3.04 ± 0.046	4.275AsnLeu: 4.275 ± 0.053	1.233AsnMet: 1.233 ± 0.029	2.17AsnAsn: 2.17 ± 0.05	2.342AsnPro: 2.342 ± 0.042	2.12AsnGln: 2.12 ± 0.042	2.125AsnArg: 2.125 ± 0.033	2.589AsnSer: 2.589 ± 0.048	2.294AsnThr: 2.294 ± 0.044	3.03AsnVal: 3.03 ± 0.048	0.597AsnTrp: 0.597 ± 0.02	1.646AsnTyr: 1.646 ± 0.034	0.001AsnXaa: 0.001 ± 0.001
Pro	2.42ProAla: 2.42 ± 0.046	0.234ProCys: 0.234 ± 0.012	1.964ProAsp: 1.964 ± 0.036	3.097ProGlu: 3.097 ± 0.057	2.039ProPhe: 2.039 ± 0.039	2.456ProGly: 2.456 ± 0.053	0.767ProHis: 0.767 ± 0.02	2.941ProIle: 2.941 ± 0.049	2.228ProLys: 2.228 ± 0.038	3.438ProLeu: 3.438 ± 0.051	0.876ProMet: 0.876 ± 0.046	1.827ProAsn: 1.827 ± 0.039	1.032ProPro: 1.032 ± 0.031	1.13ProGln: 1.13 ± 0.044	1.129ProArg: 1.129 ± 0.027	2.269ProSer: 2.269 ± 0.04	2.011ProThr: 2.011 ± 0.038	2.866ProVal: 2.866 ± 0.045	0.411ProTrp: 0.411 ± 0.018	1.456ProTyr: 1.456 ± 0.035	0.003ProXaa: 0.003 ± 0.002
Gln	2.535GlnAla: 2.535 ± 0.044	0.211GlnCys: 0.211 ± 0.012	1.601GlnAsp: 1.601 ± 0.032	2.8GlnGlu: 2.8 ± 0.054	1.675GlnPhe: 1.675 ± 0.033	2.292GlnGly: 2.292 ± 0.082	0.702GlnHis: 0.702 ± 0.021	2.648GlnIle: 2.648 ± 0.045	2.551GlnLys: 2.551 ± 0.045	3.913GlnLeu: 3.913 ± 0.056	1.096GlnMet: 1.096 ± 0.027	1.676GlnAsn: 1.676 ± 0.033	1.225GlnPro: 1.225 ± 0.03	1.686GlnGln: 1.686 ± 0.037	1.401GlnArg: 1.401 ± 0.03	1.995GlnSer: 1.995 ± 0.035	1.985GlnThr: 1.985 ± 0.037	2.392GlnVal: 2.392 ± 0.042	0.411GlnTrp: 0.411 ± 0.015	1.377GlnTyr: 1.377 ± 0.033	0.002GlnXaa: 0.002 ± 0.002
Arg	2.365ArgAla: 2.365 ± 0.04	0.27ArgCys: 0.27 ± 0.015	2.042ArgAsp: 2.042 ± 0.036	3.13ArgGlu: 3.13 ± 0.055	1.979ArgPhe: 1.979 ± 0.037	2.367ArgGly: 2.367 ± 0.041	0.763ArgHis: 0.763 ± 0.021	3.114ArgIle: 3.114 ± 0.041	3.097ArgLys: 3.097 ± 0.05	3.987ArgLeu: 3.987 ± 0.062	1.25ArgMet: 1.25 ± 0.028	2.053ArgAsn: 2.053 ± 0.036	1.301ArgPro: 1.301 ± 0.028	1.427ArgGln: 1.427 ± 0.032	1.748ArgArg: 1.748 ± 0.039	2.165ArgSer: 2.165 ± 0.039	2.031ArgThr: 2.031 ± 0.037	2.654ArgVal: 2.654 ± 0.042	0.412ArgTrp: 0.412 ± 0.019	1.571ArgTyr: 1.571 ± 0.04	0.007ArgXaa: 0.007 ± 0.003
Ser	3.617SerAla: 3.617 ± 0.049	0.458SerCys: 0.458 ± 0.019	2.778SerAsp: 2.778 ± 0.046	3.967SerGlu: 3.967 ± 0.051	3.422SerPhe: 3.422 ± 0.056	4.498SerGly: 4.498 ± 0.06	1.222SerHis: 1.222 ± 0.032	5.115SerIle: 5.115 ± 0.061	3.909SerLys: 3.909 ± 0.051	6.139SerLeu: 6.139 ± 0.073	1.652SerMet: 1.652 ± 0.032	2.69SerAsn: 2.69 ± 0.046	2.117SerPro: 2.117 ± 0.039	1.948SerGln: 1.948 ± 0.035	2.329SerArg: 2.329 ± 0.045	3.915SerSer: 3.915 ± 0.054	3.196SerThr: 3.196 ± 0.046	4.2SerVal: 4.2 ± 0.052	0.66SerTrp: 0.66 ± 0.021	2.216SerTyr: 2.216 ± 0.038	0.004SerXaa: 0.004 ± 0.001
Thr	4.007ThrAla: 4.007 ± 0.052	0.385ThrCys: 0.385 ± 0.014	2.668ThrAsp: 2.668 ± 0.048	3.577ThrGlu: 3.577 ± 0.055	2.647ThrPhe: 2.647 ± 0.044	4.273ThrGly: 4.273 ± 0.063	1.054ThrHis: 1.054 ± 0.028	4.686ThrIle: 4.686 ± 0.066	3.367ThrLys: 3.367 ± 0.04	5.336ThrLeu: 5.336 ± 0.066	1.286ThrMet: 1.286 ± 0.034	2.583ThrAsn: 2.583 ± 0.047	2.325ThrPro: 2.325 ± 0.043	1.417ThrGln: 1.417 ± 0.032	1.872ThrArg: 1.872 ± 0.035	3.257ThrSer: 3.257 ± 0.043	3.03ThrThr: 3.03 ± 0.055	4.234ThrVal: 4.234 ± 0.061	0.565ThrTrp: 0.565 ± 0.024	1.974ThrTyr: 1.974 ± 0.035	0.004ThrXaa: 0.004 ± 0.002
Val	4.599ValAla: 4.599 ± 0.058	0.617ValCys: 0.617 ± 0.021	3.553ValAsp: 3.553 ± 0.051	4.909ValGlu: 4.909 ± 0.064	3.305ValPhe: 3.305 ± 0.053	4.592ValGly: 4.592 ± 0.061	1.314ValHis: 1.314 ± 0.029	5.743ValIle: 5.743 ± 0.062	4.753ValLys: 4.753 ± 0.061	6.83ValLeu: 6.83 ± 0.077	1.8ValMet: 1.8 ± 0.041	3.333ValAsn: 3.333 ± 0.051	2.765ValPro: 2.765 ± 0.044	2.263ValGln: 2.263 ± 0.043	2.573ValArg: 2.573 ± 0.043	4.539ValSer: 4.539 ± 0.062	4.16ValThr: 4.16 ± 0.068	4.88ValVal: 4.88 ± 0.071	0.648ValTrp: 0.648 ± 0.023	2.364ValTyr: 2.364 ± 0.042	0.003ValXaa: 0.003 ± 0.001
Trp	0.682TrpAla: 0.682 ± 0.024	0.075TrpCys: 0.075 ± 0.007	0.557TrpAsp: 0.557 ± 0.019	0.728TrpGlu: 0.728 ± 0.022	0.523TrpPhe: 0.523 ± 0.02	0.777TrpGly: 0.777 ± 0.025	0.229TrpHis: 0.229 ± 0.012	0.795TrpIle: 0.795 ± 0.026	0.801TrpLys: 0.801 ± 0.024	1.139TrpLeu: 1.139 ± 0.03	0.351TrpMet: 0.351 ± 0.015	0.591TrpAsn: 0.591 ± 0.022	0.274TrpPro: 0.274 ± 0.014	0.377TrpGln: 0.377 ± 0.016	0.427TrpArg: 0.427 ± 0.017	0.674TrpSer: 0.674 ± 0.018	0.555TrpThr: 0.555 ± 0.021	0.721TrpVal: 0.721 ± 0.021	0.148TrpTrp: 0.148 ± 0.01	0.377TrpTyr: 0.377 ± 0.019	0.003TrpXaa: 0.003 ± 0.001
Tyr	1.996TyrAla: 1.996 ± 0.034	0.339TyrCys: 0.339 ± 0.015	1.851TyrAsp: 1.851 ± 0.04	2.476TyrGlu: 2.476 ± 0.041	1.969TyrPhe: 1.969 ± 0.039	2.516TyrGly: 2.516 ± 0.043	0.892TyrHis: 0.892 ± 0.028	2.527TyrIle: 2.527 ± 0.038	2.186TyrLys: 2.186 ± 0.045	3.777TyrLeu: 3.777 ± 0.052	0.88TyrMet: 0.88 ± 0.024	1.569TyrAsn: 1.569 ± 0.037	1.422TyrPro: 1.422 ± 0.034	1.672TyrGln: 1.672 ± 0.03	1.687TyrArg: 1.687 ± 0.037	2.237TyrSer: 2.237 ± 0.042	1.906TyrThr: 1.906 ± 0.039	2.145TyrVal: 2.145 ± 0.038	0.421TyrTrp: 0.421 ± 0.018	1.541TyrTyr: 1.541 ± 0.034	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.007XaaAla: 0.007 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.001XaaPhe: 0.001 ± 0.001	0.005XaaGly: 0.005 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.005XaaIle: 0.005 ± 0.002	0.004XaaLys: 0.004 ± 0.002	0.006XaaLeu: 0.006 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.003XaaPro: 0.003 ± 0.001	0.005XaaGln: 0.005 ± 0.003	0.003XaaArg: 0.003 ± 0.001	0.004XaaSer: 0.004 ± 0.002	0.003XaaThr: 0.003 ± 0.001	0.004XaaVal: 0.004 ± 0.002	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5021 proteins (1505097 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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