Amino acid dipepetide frequency for Tunisvirus fontaine2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.825AlaAla: 2.825 ± 0.229
1.187AlaCys: 1.187 ± 0.114
2.24AlaAsp: 2.24 ± 0.136
3.923AlaGlu: 3.923 ± 0.177
2.736AlaPhe: 2.736 ± 0.154
2.205AlaGly: 2.205 ± 0.158
0.841AlaHis: 0.841 ± 0.086
2.621AlaIle: 2.621 ± 0.168
4.667AlaLys: 4.667 ± 0.208
5.224AlaLeu: 5.224 ± 0.226
1.213AlaMet: 1.213 ± 0.107
2.152AlaAsn: 2.152 ± 0.146
1.868AlaPro: 1.868 ± 0.123
1.674AlaGln: 1.674 ± 0.135
2.692AlaArg: 2.692 ± 0.161
3.967AlaSer: 3.967 ± 0.224
2.931AlaThr: 2.931 ± 0.208
3.179AlaVal: 3.179 ± 0.182
0.558AlaTrp: 0.558 ± 0.069
1.558AlaTyr: 1.558 ± 0.138
0.0AlaXaa: 0.0 ± 0.0
Cys
1.328CysAla: 1.328 ± 0.111
0.762CysCys: 0.762 ± 0.086
1.355CysAsp: 1.355 ± 0.126
1.877CysGlu: 1.877 ± 0.166
1.558CysPhe: 1.558 ± 0.131
1.691CysGly: 1.691 ± 0.148
0.354CysHis: 0.354 ± 0.052
1.142CysIle: 1.142 ± 0.094
1.895CysLys: 1.895 ± 0.157
2.152CysLeu: 2.152 ± 0.139
0.469CysMet: 0.469 ± 0.064
0.806CysAsn: 0.806 ± 0.086
1.417CysPro: 1.417 ± 0.134
0.7CysGln: 0.7 ± 0.083
1.249CysArg: 1.249 ± 0.11
2.134CysSer: 2.134 ± 0.149
1.142CysThr: 1.142 ± 0.105
1.603CysVal: 1.603 ± 0.137
0.31CysTrp: 0.31 ± 0.046
0.744CysTyr: 0.744 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
2.639AspAla: 2.639 ± 0.188
1.302AspCys: 1.302 ± 0.103
2.701AspAsp: 2.701 ± 0.194
4.507AspGlu: 4.507 ± 0.239
3.144AspPhe: 3.144 ± 0.176
4.242AspGly: 4.242 ± 0.218
0.567AspHis: 0.567 ± 0.072
3.932AspIle: 3.932 ± 0.182
3.471AspLys: 3.471 ± 0.158
3.259AspLeu: 3.259 ± 0.174
0.859AspMet: 0.859 ± 0.078
1.886AspAsn: 1.886 ± 0.144
1.718AspPro: 1.718 ± 0.131
1.018AspGln: 1.018 ± 0.1
2.169AspArg: 2.169 ± 0.154
3.179AspSer: 3.179 ± 0.161
2.24AspThr: 2.24 ± 0.151
4.109AspVal: 4.109 ± 0.237
0.788AspTrp: 0.788 ± 0.091
1.558AspTyr: 1.558 ± 0.117
0.0AspXaa: 0.0 ± 0.0
Glu
3.896GluAla: 3.896 ± 0.203
1.603GluCys: 1.603 ± 0.126
4.215GluAsp: 4.215 ± 0.214
9.386GluGlu: 9.386 ± 0.42
4.472GluPhe: 4.472 ± 0.199
4.472GluGly: 4.472 ± 0.283
1.656GluHis: 1.656 ± 0.143
4.277GluIle: 4.277 ± 0.21
10.112GluLys: 10.112 ± 0.381
6.73GluLeu: 6.73 ± 0.264
2.24GluMet: 2.24 ± 0.141
4.365GluAsn: 4.365 ± 0.204
1.948GluPro: 1.948 ± 0.148
2.931GluGln: 2.931 ± 0.166
5.791GluArg: 5.791 ± 0.222
4.029GluSer: 4.029 ± 0.192
4.968GluThr: 4.968 ± 0.214
4.073GluVal: 4.073 ± 0.173
1.222GluTrp: 1.222 ± 0.115
3.055GluTyr: 3.055 ± 0.172
0.0GluXaa: 0.0 ± 0.0
Phe
3.631PheAla: 3.631 ± 0.179
1.851PheCys: 1.851 ± 0.149
2.993PheAsp: 2.993 ± 0.14
4.489PheGlu: 4.489 ± 0.199
3.09PhePhe: 3.09 ± 0.18
3.94PheGly: 3.94 ± 0.176
1.001PheHis: 1.001 ± 0.095
2.116PheIle: 2.116 ± 0.146
2.028PheLys: 2.028 ± 0.122
6.508PheLeu: 6.508 ± 0.273
1.054PheMet: 1.054 ± 0.091
1.098PheAsn: 1.098 ± 0.094
2.364PhePro: 2.364 ± 0.142
1.798PheGln: 1.798 ± 0.117
2.94PheArg: 2.94 ± 0.168
4.959PheSer: 4.959 ± 0.235
2.116PheThr: 2.116 ± 0.147
4.879PheVal: 4.879 ± 0.23
1.133PheTrp: 1.133 ± 0.101
1.744PheTyr: 1.744 ± 0.132
0.0PheXaa: 0.0 ± 0.0
Gly
2.878GlyAla: 2.878 ± 0.179
1.496GlyCys: 1.496 ± 0.138
3.161GlyAsp: 3.161 ± 0.204
4.897GlyGlu: 4.897 ± 0.246
2.674GlyPhe: 2.674 ± 0.137
3.064GlyGly: 3.064 ± 0.195
1.045GlyHis: 1.045 ± 0.108
3.701GlyIle: 3.701 ± 0.217
7.057GlyLys: 7.057 ± 0.276
4.144GlyLeu: 4.144 ± 0.228
1.443GlyMet: 1.443 ± 0.112
2.639GlyAsn: 2.639 ± 0.175
1.585GlyPro: 1.585 ± 0.104
2.116GlyGln: 2.116 ± 0.155
3.365GlyArg: 3.365 ± 0.195
4.091GlySer: 4.091 ± 0.199
3.985GlyThr: 3.985 ± 0.22
4.383GlyVal: 4.383 ± 0.248
0.797GlyTrp: 0.797 ± 0.088
2.391GlyTyr: 2.391 ± 0.138
0.0GlyXaa: 0.0 ± 0.0
His
0.788HisAla: 0.788 ± 0.075
0.567HisCys: 0.567 ± 0.078
0.584HisAsp: 0.584 ± 0.074
1.24HisGlu: 1.24 ± 0.113
1.036HisPhe: 1.036 ± 0.1
2.01HisGly: 2.01 ± 0.169
0.381HisHis: 0.381 ± 0.065
1.178HisIle: 1.178 ± 0.099
1.603HisLys: 1.603 ± 0.122
1.736HisLeu: 1.736 ± 0.119
0.319HisMet: 0.319 ± 0.055
0.638HisAsn: 0.638 ± 0.069
0.956HisPro: 0.956 ± 0.09
0.664HisGln: 0.664 ± 0.073
1.169HisArg: 1.169 ± 0.09
1.541HisSer: 1.541 ± 0.125
0.77HisThr: 0.77 ± 0.085
0.85HisVal: 0.85 ± 0.099
0.275HisTrp: 0.275 ± 0.057
0.558HisTyr: 0.558 ± 0.068
0.0HisXaa: 0.0 ± 0.0
Ile
2.896IleAla: 2.896 ± 0.162
1.195IleCys: 1.195 ± 0.109
2.285IleAsp: 2.285 ± 0.127
3.214IleGlu: 3.214 ± 0.168
3.267IlePhe: 3.267 ± 0.166
2.949IleGly: 2.949 ± 0.188
1.133IleHis: 1.133 ± 0.106
2.329IleIle: 2.329 ± 0.141
3.276IleLys: 3.276 ± 0.147
5.641IleLeu: 5.641 ± 0.247
0.974IleMet: 0.974 ± 0.101
1.435IleAsn: 1.435 ± 0.102
3.02IlePro: 3.02 ± 0.166
1.966IleGln: 1.966 ± 0.134
2.78IleArg: 2.78 ± 0.155
4.844IleSer: 4.844 ± 0.189
2.045IleThr: 2.045 ± 0.13
3.436IleVal: 3.436 ± 0.194
0.85IleTrp: 0.85 ± 0.09
1.417IleTyr: 1.417 ± 0.117
0.0IleXaa: 0.0 ± 0.0
Lys
4.286LysAla: 4.286 ± 0.229
1.452LysCys: 1.452 ± 0.147
4.897LysAsp: 4.897 ± 0.189
8.474LysGlu: 8.474 ± 0.352
3.852LysPhe: 3.852 ± 0.196
4.675LysGly: 4.675 ± 0.204
2.09LysHis: 2.09 ± 0.158
4.392LysIle: 4.392 ± 0.166
11.547LysLys: 11.547 ± 0.574
6.562LysLeu: 6.562 ± 0.251
1.727LysMet: 1.727 ± 0.121
4.489LysAsn: 4.489 ± 0.189
2.214LysPro: 2.214 ± 0.137
2.338LysGln: 2.338 ± 0.148
5.534LysArg: 5.534 ± 0.204
4.64LysSer: 4.64 ± 0.219
5.428LysThr: 5.428 ± 0.24
4.95LysVal: 4.95 ± 0.189
0.974LysTrp: 0.974 ± 0.092
3.639LysTyr: 3.639 ± 0.167
0.0LysXaa: 0.0 ± 0.0
Leu
4.782LeuAla: 4.782 ± 0.191
2.718LeuCys: 2.718 ± 0.189
4.242LeuAsp: 4.242 ± 0.178
8.209LeuGlu: 8.209 ± 0.303
5.419LeuPhe: 5.419 ± 0.21
5.384LeuGly: 5.384 ± 0.243
1.567LeuHis: 1.567 ± 0.128
2.851LeuIle: 2.851 ± 0.191
6.066LeuLys: 6.066 ± 0.235
8.846LeuLeu: 8.846 ± 0.315
1.727LeuMet: 1.727 ± 0.133
2.763LeuAsn: 2.763 ± 0.154
4.543LeuPro: 4.543 ± 0.215
3.356LeuGln: 3.356 ± 0.297
5.038LeuArg: 5.038 ± 0.219
7.916LeuSer: 7.916 ± 0.319
3.259LeuThr: 3.259 ± 0.164
6.136LeuVal: 6.136 ± 0.267
1.789LeuTrp: 1.789 ± 0.126
2.417LeuTyr: 2.417 ± 0.158
0.0LeuXaa: 0.0 ± 0.0
Met
1.125MetAla: 1.125 ± 0.096
0.531MetCys: 0.531 ± 0.075
1.098MetAsp: 1.098 ± 0.097
2.161MetGlu: 2.161 ± 0.138
1.133MetPhe: 1.133 ± 0.108
1.337MetGly: 1.337 ± 0.131
0.425MetHis: 0.425 ± 0.059
0.646MetIle: 0.646 ± 0.07
1.567MetLys: 1.567 ± 0.121
1.488MetLeu: 1.488 ± 0.112
0.584MetMet: 0.584 ± 0.068
1.133MetAsn: 1.133 ± 0.1
0.576MetPro: 0.576 ± 0.066
0.965MetGln: 0.965 ± 0.09
0.912MetArg: 0.912 ± 0.088
2.169MetSer: 2.169 ± 0.144
1.275MetThr: 1.275 ± 0.116
1.063MetVal: 1.063 ± 0.076
0.239MetTrp: 0.239 ± 0.049
0.664MetTyr: 0.664 ± 0.069
0.0MetXaa: 0.0 ± 0.0
Asn
2.125AsnAla: 2.125 ± 0.146
0.877AsnCys: 0.877 ± 0.1
1.39AsnAsp: 1.39 ± 0.107
2.639AsnGlu: 2.639 ± 0.147
2.63AsnPhe: 2.63 ± 0.16
3.117AsnGly: 3.117 ± 0.192
0.673AsnHis: 0.673 ± 0.078
3.471AsnIle: 3.471 ± 0.174
3.232AsnLys: 3.232 ± 0.202
3.214AsnLeu: 3.214 ± 0.136
0.868AsnMet: 0.868 ± 0.084
1.532AsnAsn: 1.532 ± 0.135
2.125AsnPro: 2.125 ± 0.167
0.744AsnGln: 0.744 ± 0.104
1.55AsnArg: 1.55 ± 0.125
2.975AsnSer: 2.975 ± 0.18
2.223AsnThr: 2.223 ± 0.194
2.825AsnVal: 2.825 ± 0.188
0.505AsnTrp: 0.505 ± 0.062
1.523AsnTyr: 1.523 ± 0.094
0.0AsnXaa: 0.0 ± 0.0
Pro
1.364ProAla: 1.364 ± 0.117
0.744ProCys: 0.744 ± 0.082
2.143ProAsp: 2.143 ± 0.158
4.126ProGlu: 4.126 ± 0.179
1.984ProPhe: 1.984 ± 0.111
2.231ProGly: 2.231 ± 0.133
0.762ProHis: 0.762 ± 0.086
1.984ProIle: 1.984 ± 0.14
3.427ProLys: 3.427 ± 0.231
3.161ProLeu: 3.161 ± 0.155
0.753ProMet: 0.753 ± 0.083
1.842ProAsn: 1.842 ± 0.139
1.753ProPro: 1.753 ± 0.185
1.452ProGln: 1.452 ± 0.114
2.107ProArg: 2.107 ± 0.135
2.692ProSer: 2.692 ± 0.163
1.939ProThr: 1.939 ± 0.149
2.205ProVal: 2.205 ± 0.156
0.584ProTrp: 0.584 ± 0.082
1.328ProTyr: 1.328 ± 0.115
0.0ProXaa: 0.0 ± 0.0
Gln
1.576GlnAla: 1.576 ± 0.129
0.39GlnCys: 0.39 ± 0.059
1.408GlnAsp: 1.408 ± 0.106
3.135GlnGlu: 3.135 ± 0.178
0.974GlnPhe: 0.974 ± 0.093
1.833GlnGly: 1.833 ± 0.185
0.593GlnHis: 0.593 ± 0.073
1.736GlnIle: 1.736 ± 0.126
4.41GlnLys: 4.41 ± 0.182
2.161GlnLeu: 2.161 ± 0.148
0.983GlnMet: 0.983 ± 0.093
1.762GlnAsn: 1.762 ± 0.11
0.85GlnPro: 0.85 ± 0.093
1.107GlnGln: 1.107 ± 0.132
2.382GlnArg: 2.382 ± 0.161
1.62GlnSer: 1.62 ± 0.11
2.107GlnThr: 2.107 ± 0.147
2.409GlnVal: 2.409 ± 0.195
0.46GlnTrp: 0.46 ± 0.05
1.054GlnTyr: 1.054 ± 0.105
0.0GlnXaa: 0.0 ± 0.0
Arg
2.479ArgAla: 2.479 ± 0.167
1.178ArgCys: 1.178 ± 0.12
2.896ArgAsp: 2.896 ± 0.177
5.49ArgGlu: 5.49 ± 0.225
2.285ArgPhe: 2.285 ± 0.142
2.922ArgGly: 2.922 ± 0.175
1.222ArgHis: 1.222 ± 0.099
3.126ArgIle: 3.126 ± 0.202
5.464ArgLys: 5.464 ± 0.228
4.976ArgLeu: 4.976 ± 0.231
1.266ArgMet: 1.266 ± 0.123
2.639ArgAsn: 2.639 ± 0.166
1.674ArgPro: 1.674 ± 0.135
2.116ArgGln: 2.116 ± 0.126
3.055ArgArg: 3.055 ± 0.162
2.904ArgSer: 2.904 ± 0.172
2.86ArgThr: 2.86 ± 0.178
4.064ArgVal: 4.064 ± 0.199
0.629ArgTrp: 0.629 ± 0.079
1.771ArgTyr: 1.771 ± 0.115
0.0ArgXaa: 0.0 ± 0.0
Ser
3.391SerAla: 3.391 ± 0.181
2.249SerCys: 2.249 ± 0.15
3.524SerAsp: 3.524 ± 0.178
5.703SerGlu: 5.703 ± 0.215
4.959SerPhe: 4.959 ± 0.232
5.278SerGly: 5.278 ± 0.236
1.452SerHis: 1.452 ± 0.128
3.276SerIle: 3.276 ± 0.148
5.402SerLys: 5.402 ± 0.239
7.589SerLeu: 7.589 ± 0.246
1.257SerMet: 1.257 ± 0.107
2.568SerAsn: 2.568 ± 0.169
2.772SerPro: 2.772 ± 0.185
2.665SerGln: 2.665 ± 0.146
3.471SerArg: 3.471 ± 0.19
6.615SerSer: 6.615 ± 0.292
3.276SerThr: 3.276 ± 0.186
4.879SerVal: 4.879 ± 0.185
1.257SerTrp: 1.257 ± 0.103
2.019SerTyr: 2.019 ± 0.152
0.0SerXaa: 0.0 ± 0.0
Thr
2.267ThrAla: 2.267 ± 0.162
1.426ThrCys: 1.426 ± 0.123
2.285ThrAsp: 2.285 ± 0.167
3.551ThrGlu: 3.551 ± 0.177
2.913ThrPhe: 2.913 ± 0.145
3.285ThrGly: 3.285 ± 0.215
0.779ThrHis: 0.779 ± 0.081
2.515ThrIle: 2.515 ± 0.166
4.799ThrLys: 4.799 ± 0.243
5.021ThrLeu: 5.021 ± 0.251
1.125ThrMet: 1.125 ± 0.099
2.453ThrAsn: 2.453 ± 0.232
2.462ThrPro: 2.462 ± 0.174
1.576ThrGln: 1.576 ± 0.115
3.073ThrArg: 3.073 ± 0.168
3.383ThrSer: 3.383 ± 0.172
3.17ThrThr: 3.17 ± 0.191
3.161ThrVal: 3.161 ± 0.164
0.638ThrTrp: 0.638 ± 0.077
1.514ThrTyr: 1.514 ± 0.112
0.0ThrXaa: 0.0 ± 0.0
Val
3.374ValAla: 3.374 ± 0.22
1.957ValCys: 1.957 ± 0.161
3.073ValAsp: 3.073 ± 0.173
4.746ValGlu: 4.746 ± 0.237
4.427ValPhe: 4.427 ± 0.206
3.117ValGly: 3.117 ± 0.213
1.452ValHis: 1.452 ± 0.117
3.179ValIle: 3.179 ± 0.157
4.304ValLys: 4.304 ± 0.191
6.88ValLeu: 6.88 ± 0.289
1.231ValMet: 1.231 ± 0.101
1.957ValAsn: 1.957 ± 0.132
3.205ValPro: 3.205 ± 0.187
2.178ValGln: 2.178 ± 0.126
3.188ValArg: 3.188 ± 0.173
6.136ValSer: 6.136 ± 0.219
3.055ValThr: 3.055 ± 0.196
4.861ValVal: 4.861 ± 0.212
1.018ValTrp: 1.018 ± 0.09
2.462ValTyr: 2.462 ± 0.155
0.0ValXaa: 0.0 ± 0.0
Trp
0.593TrpAla: 0.593 ± 0.069
0.407TrpCys: 0.407 ± 0.071
0.832TrpAsp: 0.832 ± 0.083
1.231TrpGlu: 1.231 ± 0.12
1.063TrpPhe: 1.063 ± 0.098
0.549TrpGly: 0.549 ± 0.088
0.275TrpHis: 0.275 ± 0.05
0.735TrpIle: 0.735 ± 0.088
1.558TrpLys: 1.558 ± 0.132
1.16TrpLeu: 1.16 ± 0.105
0.372TrpMet: 0.372 ± 0.057
0.7TrpAsn: 0.7 ± 0.088
0.31TrpPro: 0.31 ± 0.046
0.434TrpGln: 0.434 ± 0.057
0.965TrpArg: 0.965 ± 0.093
1.204TrpSer: 1.204 ± 0.107
0.806TrpThr: 0.806 ± 0.088
0.797TrpVal: 0.797 ± 0.094
0.275TrpTrp: 0.275 ± 0.049
0.682TrpTyr: 0.682 ± 0.086
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.019TyrAla: 2.019 ± 0.135
0.797TyrCys: 0.797 ± 0.082
1.966TyrAsp: 1.966 ± 0.149
2.444TyrGlu: 2.444 ± 0.151
2.116TyrPhe: 2.116 ± 0.15
2.612TyrGly: 2.612 ± 0.156
0.593TyrHis: 0.593 ± 0.068
1.744TyrIle: 1.744 ± 0.136
2.276TyrLys: 2.276 ± 0.139
2.577TyrLeu: 2.577 ± 0.15
0.62TyrMet: 0.62 ± 0.078
1.381TyrAsn: 1.381 ± 0.114
1.222TyrPro: 1.222 ± 0.107
1.151TyrGln: 1.151 ± 0.084
1.585TyrArg: 1.585 ± 0.112
2.612TyrSer: 2.612 ± 0.142
1.798TyrThr: 1.798 ± 0.127
1.939TyrVal: 1.939 ± 0.128
0.646TyrTrp: 0.646 ± 0.068
0.956TyrTyr: 0.956 ± 0.094
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 484 proteins (112932 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski