Nesterenkonia natronophila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Nesterenkonia

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A4F223|A0A3A4F223_9MICC Phospho-sugar mutase OS=Nesterenkonia natronophila OX=2174932 GN=D3250_08490 PE=3 SV=1
MM1 pKa = 7.34AAVGVVVLVVRR12 pKa = 11.84TCSTEE17 pKa = 3.96TEE19 pKa = 4.11PDD21 pKa = 3.3PTEE24 pKa = 4.7LGFPPAPVQEE34 pKa = 4.15QPVLSQATGQAGEE47 pKa = 4.21VPITDD52 pKa = 3.97LVDD55 pKa = 3.85SEE57 pKa = 4.67WVKK60 pKa = 10.08RR61 pKa = 11.84TAEE64 pKa = 3.93RR65 pKa = 11.84AEE67 pKa = 4.02IPHH70 pKa = 6.81RR71 pKa = 11.84AMEE74 pKa = 4.53SYY76 pKa = 10.98AGAAAYY82 pKa = 10.22AEE84 pKa = 4.54LTDD87 pKa = 5.03PDD89 pKa = 4.88CQLGWNTLAGIGQVEE104 pKa = 4.54SLHH107 pKa = 6.09GAFGGSSIGEE117 pKa = 4.41DD118 pKa = 3.52GVVDD122 pKa = 3.93PPIIGVALDD131 pKa = 3.93GSDD134 pKa = 3.44GLMEE138 pKa = 5.74IPDD141 pKa = 4.3TDD143 pKa = 5.45DD144 pKa = 4.2GDD146 pKa = 5.18LDD148 pKa = 6.14GDD150 pKa = 4.49DD151 pKa = 3.59EE152 pKa = 4.42WDD154 pKa = 3.59RR155 pKa = 11.84AVGPMQFIPTTWEE168 pKa = 3.61AFGQDD173 pKa = 3.8GNLSGQANIHH183 pKa = 5.91QYY185 pKa = 11.69DD186 pKa = 3.92DD187 pKa = 3.66AALAAAIYY195 pKa = 10.34LCDD198 pKa = 3.99SGDD201 pKa = 4.23DD202 pKa = 3.77LTEE205 pKa = 4.09DD206 pKa = 4.37RR207 pKa = 11.84GWNDD211 pKa = 4.45AIAAYY216 pKa = 9.52NQSIEE221 pKa = 4.18YY222 pKa = 9.67AHH224 pKa = 7.12DD225 pKa = 3.65VAHH228 pKa = 6.64HH229 pKa = 6.6AEE231 pKa = 4.66HH232 pKa = 6.18YY233 pKa = 10.88LKK235 pKa = 11.0DD236 pKa = 3.59EE237 pKa = 4.28

Molecular weight: 25.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A4F4Y8|A0A3A4F4Y8_9MICC D-2-hydroxyacid dehydrogenase OS=Nesterenkonia natronophila OX=2174932 GN=D3250_02875 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84KK16 pKa = 8.87HH17 pKa = 4.83GFRR20 pKa = 11.84SRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SIISARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84SGRR40 pKa = 11.84QKK42 pKa = 10.95LSAA45 pKa = 4.16

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2262

0

2262

764907

32

2202

338.2

36.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.835 ± 0.056

0.627 ± 0.011

5.821 ± 0.042

6.793 ± 0.055

3.125 ± 0.03

8.417 ± 0.043

2.405 ± 0.023

4.425 ± 0.036

2.472 ± 0.032

10.109 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.018

2.255 ± 0.024

5.282 ± 0.035

3.624 ± 0.028

6.837 ± 0.046

6.094 ± 0.034

5.944 ± 0.031

8.266 ± 0.043

1.438 ± 0.021

2.087 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski