Pelagibacter phage HTVC021P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; unclassified Pelagivirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NT31|A0A4Y1NT31_9CAUD Single-stranded DNA-binding protein OS=Pelagibacter phage HTVC021P OX=2282994 GN=P021_gp17 PE=4 SV=1
MM1 pKa = 7.28NFKK4 pKa = 10.57KK5 pKa = 10.53EE6 pKa = 3.77YY7 pKa = 10.68DD8 pKa = 3.18EE9 pKa = 4.61WLDD12 pKa = 3.59EE13 pKa = 4.03LHH15 pKa = 7.04PLTGTSCNPFSVLLEE30 pKa = 4.34EE31 pKa = 5.27GDD33 pKa = 4.28PIAYY37 pKa = 9.78NCGYY41 pKa = 10.98DD42 pKa = 4.25DD43 pKa = 5.56FLDD46 pKa = 3.69ATGRR50 pKa = 11.84EE51 pKa = 4.06EE52 pKa = 4.72DD53 pKa = 3.37

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NSJ6|A0A4Y1NSJ6_9CAUD Lysozyme OS=Pelagibacter phage HTVC021P OX=2282994 GN=P021_gp60 PE=4 SV=1
MM1 pKa = 7.51PRR3 pKa = 11.84PVRR6 pKa = 11.84KK7 pKa = 9.17WIVRR11 pKa = 11.84LRR13 pKa = 11.84MWYY16 pKa = 9.94ADD18 pKa = 2.97IRR20 pKa = 11.84GHH22 pKa = 6.67HH23 pKa = 6.1GKK25 pKa = 9.84RR26 pKa = 11.84WDD28 pKa = 3.77YY29 pKa = 10.91EE30 pKa = 4.02PSEE33 pKa = 4.74HH34 pKa = 7.05YY35 pKa = 9.52MGRR38 pKa = 11.84NKK40 pKa = 10.16HH41 pKa = 5.59KK42 pKa = 10.78

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13339

40

1261

222.3

24.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.317 ± 0.554

0.877 ± 0.13

5.84 ± 0.172

6.74 ± 0.62

3.861 ± 0.173

6.447 ± 0.309

1.934 ± 0.176

6.53 ± 0.242

8.689 ± 0.649

7.872 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.225

6.072 ± 0.474

2.916 ± 0.169

3.509 ± 0.215

3.846 ± 0.303

6.942 ± 0.472

7.429 ± 0.673

5.705 ± 0.419

1.312 ± 0.131

3.583 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski