Amino acid dipepetide frequency for Pelagibacter phage HTVC021P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.049AlaAla: 4.049 ± 0.921
0.6AlaCys: 0.6 ± 0.26
5.323AlaAsp: 5.323 ± 0.716
5.623AlaGlu: 5.623 ± 1.019
2.249AlaPhe: 2.249 ± 0.425
5.623AlaGly: 5.623 ± 1.545
0.825AlaHis: 0.825 ± 0.285
5.548AlaIle: 5.548 ± 0.673
6.148AlaLys: 6.148 ± 0.807
4.948AlaLeu: 4.948 ± 0.662
2.099AlaMet: 2.099 ± 0.367
5.923AlaAsn: 5.923 ± 1.429
2.324AlaPro: 2.324 ± 0.473
1.949AlaGln: 1.949 ± 0.499
1.949AlaArg: 1.949 ± 0.368
5.323AlaSer: 5.323 ± 0.738
4.873AlaThr: 4.873 ± 1.24
3.974AlaVal: 3.974 ± 0.663
0.975AlaTrp: 0.975 ± 0.3
2.849AlaTyr: 2.849 ± 0.499
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.183
0.075CysCys: 0.075 ± 0.074
0.375CysAsp: 0.375 ± 0.178
0.525CysGlu: 0.525 ± 0.222
0.375CysPhe: 0.375 ± 0.136
0.45CysGly: 0.45 ± 0.2
0.375CysHis: 0.375 ± 0.152
0.675CysIle: 0.675 ± 0.274
0.9CysLys: 0.9 ± 0.332
0.675CysLeu: 0.675 ± 0.322
0.15CysMet: 0.15 ± 0.102
0.6CysAsn: 0.6 ± 0.268
0.225CysPro: 0.225 ± 0.15
0.3CysGln: 0.3 ± 0.131
0.45CysArg: 0.45 ± 0.189
0.525CysSer: 0.525 ± 0.217
0.9CysThr: 0.9 ± 0.266
0.3CysVal: 0.3 ± 0.144
0.15CysTrp: 0.15 ± 0.13
0.3CysTyr: 0.3 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
3.899AspAla: 3.899 ± 0.771
0.45AspCys: 0.45 ± 0.213
3.524AspAsp: 3.524 ± 0.506
3.524AspGlu: 3.524 ± 0.739
2.399AspPhe: 2.399 ± 0.387
4.423AspGly: 4.423 ± 0.657
0.525AspHis: 0.525 ± 0.163
4.274AspIle: 4.274 ± 0.531
5.323AspLys: 5.323 ± 0.655
5.773AspLeu: 5.773 ± 0.584
1.724AspMet: 1.724 ± 0.381
3.149AspAsn: 3.149 ± 0.546
1.724AspPro: 1.724 ± 0.468
0.975AspGln: 0.975 ± 0.264
3.074AspArg: 3.074 ± 0.656
2.999AspSer: 2.999 ± 0.649
3.974AspThr: 3.974 ± 0.551
3.449AspVal: 3.449 ± 0.583
0.75AspTrp: 0.75 ± 0.217
2.474AspTyr: 2.474 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
5.623GluAla: 5.623 ± 1.009
0.75GluCys: 0.75 ± 0.235
3.749GluAsp: 3.749 ± 0.568
4.798GluGlu: 4.798 ± 0.839
2.249GluPhe: 2.249 ± 0.6
4.348GluGly: 4.348 ± 0.542
1.499GluHis: 1.499 ± 0.374
5.098GluIle: 5.098 ± 0.716
7.422GluLys: 7.422 ± 1.362
5.473GluLeu: 5.473 ± 0.683
1.799GluMet: 1.799 ± 0.386
3.224GluAsn: 3.224 ± 0.578
1.2GluPro: 1.2 ± 0.402
2.849GluGln: 2.849 ± 0.649
2.849GluArg: 2.849 ± 0.604
1.874GluSer: 1.874 ± 0.376
4.423GluThr: 4.423 ± 0.639
3.824GluVal: 3.824 ± 0.634
1.425GluTrp: 1.425 ± 0.443
2.924GluTyr: 2.924 ± 0.576
0.0GluXaa: 0.0 ± 0.0
Phe
1.874PheAla: 1.874 ± 0.401
0.225PheCys: 0.225 ± 0.163
3.074PheAsp: 3.074 ± 0.479
2.249PheGlu: 2.249 ± 0.463
1.35PhePhe: 1.35 ± 0.39
3.374PheGly: 3.374 ± 0.742
0.75PheHis: 0.75 ± 0.304
1.574PheIle: 1.574 ± 0.34
4.573PheLys: 4.573 ± 0.67
3.599PheLeu: 3.599 ± 0.58
1.499PheMet: 1.499 ± 0.267
1.649PheAsn: 1.649 ± 0.399
0.75PhePro: 0.75 ± 0.201
1.425PheGln: 1.425 ± 0.34
1.425PheArg: 1.425 ± 0.289
3.074PheSer: 3.074 ± 0.419
2.024PheThr: 2.024 ± 0.487
2.699PheVal: 2.699 ± 0.511
0.45PheTrp: 0.45 ± 0.188
0.975PheTyr: 0.975 ± 0.325
0.0PheXaa: 0.0 ± 0.0
Gly
3.824GlyAla: 3.824 ± 0.786
0.3GlyCys: 0.3 ± 0.144
3.899GlyAsp: 3.899 ± 0.454
4.348GlyGlu: 4.348 ± 0.679
3.599GlyPhe: 3.599 ± 0.531
3.824GlyGly: 3.824 ± 0.729
1.125GlyHis: 1.125 ± 0.281
3.974GlyIle: 3.974 ± 0.495
5.623GlyLys: 5.623 ± 0.694
4.274GlyLeu: 4.274 ± 0.538
1.499GlyMet: 1.499 ± 0.427
4.723GlyAsn: 4.723 ± 0.802
0.0GlyPro: 0.0 ± 0.0
2.699GlyGln: 2.699 ± 0.429
2.549GlyArg: 2.549 ± 0.534
4.948GlySer: 4.948 ± 0.708
6.148GlyThr: 6.148 ± 1.137
3.824GlyVal: 3.824 ± 0.591
0.9GlyTrp: 0.9 ± 0.24
2.399GlyTyr: 2.399 ± 0.48
0.0GlyXaa: 0.0 ± 0.0
His
1.499HisAla: 1.499 ± 0.368
0.375HisCys: 0.375 ± 0.176
0.9HisAsp: 0.9 ± 0.267
0.975HisGlu: 0.975 ± 0.314
0.825HisPhe: 0.825 ± 0.235
1.2HisGly: 1.2 ± 0.231
0.45HisHis: 0.45 ± 0.191
1.425HisIle: 1.425 ± 0.401
1.35HisLys: 1.35 ± 0.301
1.649HisLeu: 1.649 ± 0.486
0.6HisMet: 0.6 ± 0.216
1.05HisAsn: 1.05 ± 0.212
0.75HisPro: 0.75 ± 0.182
0.6HisGln: 0.6 ± 0.208
0.825HisArg: 0.825 ± 0.276
1.574HisSer: 1.574 ± 0.351
1.425HisThr: 1.425 ± 0.277
0.9HisVal: 0.9 ± 0.31
0.3HisTrp: 0.3 ± 0.134
0.675HisTyr: 0.675 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
5.923IleAla: 5.923 ± 0.557
0.75IleCys: 0.75 ± 0.261
4.798IleAsp: 4.798 ± 0.691
5.023IleGlu: 5.023 ± 0.852
1.949IlePhe: 1.949 ± 0.358
4.124IleGly: 4.124 ± 0.631
1.425IleHis: 1.425 ± 0.378
4.274IleIle: 4.274 ± 0.723
6.298IleLys: 6.298 ± 0.801
4.124IleLeu: 4.124 ± 0.594
1.799IleMet: 1.799 ± 0.379
3.824IleAsn: 3.824 ± 0.766
2.399IlePro: 2.399 ± 0.355
2.324IleGln: 2.324 ± 0.354
2.474IleArg: 2.474 ± 0.393
3.599IleSer: 3.599 ± 0.518
4.648IleThr: 4.648 ± 0.873
3.149IleVal: 3.149 ± 0.412
0.525IleTrp: 0.525 ± 0.2
1.874IleTyr: 1.874 ± 0.325
0.0IleXaa: 0.0 ± 0.0
Lys
6.073LysAla: 6.073 ± 1.077
0.6LysCys: 0.6 ± 0.296
4.948LysAsp: 4.948 ± 0.799
8.022LysGlu: 8.022 ± 1.248
3.449LysPhe: 3.449 ± 0.457
4.573LysGly: 4.573 ± 0.692
2.024LysHis: 2.024 ± 0.397
5.323LysIle: 5.323 ± 0.575
7.797LysLys: 7.797 ± 1.079
6.898LysLeu: 6.898 ± 0.848
2.249LysMet: 2.249 ± 0.51
3.974LysAsn: 3.974 ± 0.774
3.374LysPro: 3.374 ± 0.512
3.074LysGln: 3.074 ± 0.607
4.498LysArg: 4.498 ± 0.818
5.398LysSer: 5.398 ± 0.777
5.248LysThr: 5.248 ± 0.685
5.173LysVal: 5.173 ± 0.814
0.75LysTrp: 0.75 ± 0.238
3.449LysTyr: 3.449 ± 0.661
0.0LysXaa: 0.0 ± 0.0
Leu
5.923LeuAla: 5.923 ± 0.67
0.6LeuCys: 0.6 ± 0.26
4.873LeuAsp: 4.873 ± 0.606
5.848LeuGlu: 5.848 ± 0.869
2.399LeuPhe: 2.399 ± 0.473
3.824LeuGly: 3.824 ± 0.593
1.874LeuHis: 1.874 ± 0.449
3.974LeuIle: 3.974 ± 0.613
7.272LeuLys: 7.272 ± 0.802
6.298LeuLeu: 6.298 ± 1.016
2.174LeuMet: 2.174 ± 0.446
5.173LeuAsn: 5.173 ± 0.526
3.224LeuPro: 3.224 ± 0.485
2.849LeuGln: 2.849 ± 0.458
2.774LeuArg: 2.774 ± 0.549
5.398LeuSer: 5.398 ± 0.686
5.923LeuThr: 5.923 ± 0.56
4.798LeuVal: 4.798 ± 0.638
0.9LeuTrp: 0.9 ± 0.343
2.624LeuTyr: 2.624 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
2.999MetAla: 2.999 ± 0.472
0.375MetCys: 0.375 ± 0.176
1.05MetAsp: 1.05 ± 0.234
1.574MetGlu: 1.574 ± 0.349
0.975MetPhe: 0.975 ± 0.287
1.874MetGly: 1.874 ± 0.278
0.375MetHis: 0.375 ± 0.165
1.35MetIle: 1.35 ± 0.353
2.174MetLys: 2.174 ± 0.389
2.099MetLeu: 2.099 ± 0.635
0.75MetMet: 0.75 ± 0.26
1.125MetAsn: 1.125 ± 0.243
1.275MetPro: 1.275 ± 0.364
1.125MetGln: 1.125 ± 0.302
1.275MetArg: 1.275 ± 0.374
1.949MetSer: 1.949 ± 0.365
1.05MetThr: 1.05 ± 0.279
1.125MetVal: 1.125 ± 0.347
0.45MetTrp: 0.45 ± 0.184
0.75MetTyr: 0.75 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
4.423AsnAla: 4.423 ± 0.823
0.6AsnCys: 0.6 ± 0.216
2.774AsnAsp: 2.774 ± 0.354
3.374AsnGlu: 3.374 ± 0.346
2.774AsnPhe: 2.774 ± 0.424
3.974AsnGly: 3.974 ± 0.732
0.825AsnHis: 0.825 ± 0.258
4.049AsnIle: 4.049 ± 0.813
4.873AsnLys: 4.873 ± 0.62
4.873AsnLeu: 4.873 ± 0.525
1.2AsnMet: 1.2 ± 0.313
4.423AsnAsn: 4.423 ± 0.728
1.799AsnPro: 1.799 ± 0.293
2.024AsnGln: 2.024 ± 0.378
2.849AsnArg: 2.849 ± 0.523
4.348AsnSer: 4.348 ± 1.206
5.473AsnThr: 5.473 ± 0.834
2.999AsnVal: 2.999 ± 0.739
0.3AsnTrp: 0.3 ± 0.146
2.774AsnTyr: 2.774 ± 0.493
0.0AsnXaa: 0.0 ± 0.0
Pro
1.649ProAla: 1.649 ± 0.283
0.3ProCys: 0.3 ± 0.142
1.874ProAsp: 1.874 ± 0.417
2.549ProGlu: 2.549 ± 0.567
1.799ProPhe: 1.799 ± 0.383
0.0ProGly: 0.0 ± 0.0
0.45ProHis: 0.45 ± 0.175
1.649ProIle: 1.649 ± 0.452
2.174ProLys: 2.174 ± 0.416
2.699ProLeu: 2.699 ± 0.506
0.75ProMet: 0.75 ± 0.196
1.649ProAsn: 1.649 ± 0.333
1.275ProPro: 1.275 ± 0.4
1.05ProGln: 1.05 ± 0.296
0.75ProArg: 0.75 ± 0.238
2.999ProSer: 2.999 ± 0.415
2.774ProThr: 2.774 ± 0.441
1.724ProVal: 1.724 ± 0.356
0.0ProTrp: 0.0 ± 0.0
1.05ProTyr: 1.05 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
2.774GlnAla: 2.774 ± 0.52
0.375GlnCys: 0.375 ± 0.17
1.425GlnAsp: 1.425 ± 0.275
2.099GlnGlu: 2.099 ± 0.523
1.425GlnPhe: 1.425 ± 0.35
1.724GlnGly: 1.724 ± 0.366
0.975GlnHis: 0.975 ± 0.284
2.174GlnIle: 2.174 ± 0.438
2.999GlnLys: 2.999 ± 0.551
2.549GlnLeu: 2.549 ± 0.459
1.2GlnMet: 1.2 ± 0.354
1.799GlnAsn: 1.799 ± 0.364
0.9GlnPro: 0.9 ± 0.225
1.499GlnGln: 1.499 ± 0.286
1.275GlnArg: 1.275 ± 0.326
2.774GlnSer: 2.774 ± 0.419
3.149GlnThr: 3.149 ± 0.557
2.024GlnVal: 2.024 ± 0.458
0.675GlnTrp: 0.675 ± 0.249
1.275GlnTyr: 1.275 ± 0.274
0.0GlnXaa: 0.0 ± 0.0
Arg
2.549ArgAla: 2.549 ± 0.407
0.0ArgCys: 0.0 ± 0.0
2.924ArgAsp: 2.924 ± 0.42
2.849ArgGlu: 2.849 ± 0.534
0.975ArgPhe: 0.975 ± 0.296
2.849ArgGly: 2.849 ± 0.535
0.75ArgHis: 0.75 ± 0.278
2.774ArgIle: 2.774 ± 0.668
2.849ArgLys: 2.849 ± 0.487
3.674ArgLeu: 3.674 ± 0.729
1.35ArgMet: 1.35 ± 0.447
2.099ArgAsn: 2.099 ± 0.399
1.275ArgPro: 1.275 ± 0.276
1.125ArgGln: 1.125 ± 0.248
1.499ArgArg: 1.499 ± 0.508
2.249ArgSer: 2.249 ± 0.474
1.799ArgThr: 1.799 ± 0.424
1.574ArgVal: 1.574 ± 0.424
1.125ArgTrp: 1.125 ± 0.36
2.174ArgTyr: 2.174 ± 0.488
0.0ArgXaa: 0.0 ± 0.0
Ser
5.173SerAla: 5.173 ± 0.931
0.525SerCys: 0.525 ± 0.218
2.624SerAsp: 2.624 ± 0.4
4.274SerGlu: 4.274 ± 0.712
2.774SerPhe: 2.774 ± 0.392
5.398SerGly: 5.398 ± 0.872
1.724SerHis: 1.724 ± 0.415
5.398SerIle: 5.398 ± 0.524
5.323SerLys: 5.323 ± 0.696
4.948SerLeu: 4.948 ± 0.639
1.574SerMet: 1.574 ± 0.338
3.974SerAsn: 3.974 ± 0.775
1.649SerPro: 1.649 ± 0.348
2.774SerGln: 2.774 ± 0.563
1.649SerArg: 1.649 ± 0.348
6.223SerSer: 6.223 ± 1.311
6.148SerThr: 6.148 ± 1.114
3.749SerVal: 3.749 ± 0.683
1.35SerTrp: 1.35 ± 0.288
2.174SerTyr: 2.174 ± 0.414
0.0SerXaa: 0.0 ± 0.0
Thr
6.373ThrAla: 6.373 ± 1.272
0.6ThrCys: 0.6 ± 0.294
4.124ThrAsp: 4.124 ± 0.595
4.199ThrGlu: 4.199 ± 0.544
3.149ThrPhe: 3.149 ± 0.676
5.773ThrGly: 5.773 ± 0.674
1.275ThrHis: 1.275 ± 0.297
4.648ThrIle: 4.648 ± 0.507
4.873ThrLys: 4.873 ± 0.536
5.248ThrLeu: 5.248 ± 0.948
1.275ThrMet: 1.275 ± 0.247
5.398ThrAsn: 5.398 ± 1.355
2.399ThrPro: 2.399 ± 0.467
2.624ThrGln: 2.624 ± 0.519
2.249ThrArg: 2.249 ± 0.345
6.448ThrSer: 6.448 ± 1.397
5.923ThrThr: 5.923 ± 1.727
4.798ThrVal: 4.798 ± 1.114
0.75ThrTrp: 0.75 ± 0.215
2.174ThrTyr: 2.174 ± 0.506
0.0ThrXaa: 0.0 ± 0.0
Val
5.173ValAla: 5.173 ± 1.149
0.675ValCys: 0.675 ± 0.23
3.224ValAsp: 3.224 ± 0.455
3.524ValGlu: 3.524 ± 0.604
2.174ValPhe: 2.174 ± 0.359
3.899ValGly: 3.899 ± 0.563
1.35ValHis: 1.35 ± 0.392
3.299ValIle: 3.299 ± 0.502
4.348ValLys: 4.348 ± 0.687
4.498ValLeu: 4.498 ± 0.64
1.35ValMet: 1.35 ± 0.336
3.749ValAsn: 3.749 ± 0.764
1.649ValPro: 1.649 ± 0.311
1.874ValGln: 1.874 ± 0.346
2.099ValArg: 2.099 ± 0.44
4.573ValSer: 4.573 ± 0.553
4.049ValThr: 4.049 ± 0.678
3.599ValVal: 3.599 ± 0.511
0.525ValTrp: 0.525 ± 0.16
1.425ValTyr: 1.425 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
0.825TrpAla: 0.825 ± 0.235
0.075TrpCys: 0.075 ± 0.091
1.125TrpAsp: 1.125 ± 0.235
0.525TrpGlu: 0.525 ± 0.201
0.375TrpPhe: 0.375 ± 0.157
0.75TrpGly: 0.75 ± 0.287
0.0TrpHis: 0.0 ± 0.0
0.9TrpIle: 0.9 ± 0.242
1.125TrpLys: 1.125 ± 0.316
1.574TrpLeu: 1.574 ± 0.313
0.075TrpMet: 0.075 ± 0.087
1.125TrpAsn: 1.125 ± 0.294
0.0TrpPro: 0.0 ± 0.0
0.45TrpGln: 0.45 ± 0.165
0.825TrpArg: 0.825 ± 0.295
0.825TrpSer: 0.825 ± 0.224
1.2TrpThr: 1.2 ± 0.247
0.9TrpVal: 0.9 ± 0.305
0.075TrpTrp: 0.075 ± 0.08
0.375TrpTyr: 0.375 ± 0.205
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.099TyrAla: 2.099 ± 0.379
0.525TyrCys: 0.525 ± 0.185
1.799TyrAsp: 1.799 ± 0.32
1.499TyrGlu: 1.499 ± 0.35
1.35TyrPhe: 1.35 ± 0.326
2.474TyrGly: 2.474 ± 0.454
0.75TyrHis: 0.75 ± 0.214
2.924TyrIle: 2.924 ± 0.509
3.449TyrLys: 3.449 ± 0.591
2.849TyrLeu: 2.849 ± 0.463
0.6TyrMet: 0.6 ± 0.198
2.099TyrAsn: 2.099 ± 0.326
0.975TyrPro: 0.975 ± 0.319
1.499TyrGln: 1.499 ± 0.353
1.125TyrArg: 1.125 ± 0.342
2.324TyrSer: 2.324 ± 0.408
3.149TyrThr: 3.149 ± 0.511
2.474TyrVal: 2.474 ± 0.349
0.75TyrTrp: 0.75 ± 0.25
1.125TyrTyr: 1.125 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (13339 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski