Siphoviridae sp. ct7UA22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WBG7|A0A5Q2WBG7_9CAUD Uncharacterized protein OS=Siphoviridae sp. ct7UA22 OX=2656718 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 5.54LEE4 pKa = 4.31IGSLHH9 pKa = 6.75SGGLVVGVNPLEE21 pKa = 4.16LAPNVFNDD29 pKa = 3.32VANVEE34 pKa = 4.55FTRR37 pKa = 11.84EE38 pKa = 3.82GVRR41 pKa = 11.84PLRR44 pKa = 11.84QPVTRR49 pKa = 11.84NLDD52 pKa = 3.5VPGSRR57 pKa = 11.84LVYY60 pKa = 9.96HH61 pKa = 7.07AVDD64 pKa = 3.3TMVNGGIRR72 pKa = 11.84VVLCFCEE79 pKa = 4.2DD80 pKa = 2.59KK81 pKa = 11.23VYY83 pKa = 11.09ALRR86 pKa = 11.84GSSYY90 pKa = 11.33LDD92 pKa = 3.22VTPAGMLSSKK102 pKa = 9.09EE103 pKa = 3.8WSVTEE108 pKa = 3.76FNGYY112 pKa = 8.47VVACNGKK119 pKa = 8.84NFPYY123 pKa = 10.62YY124 pKa = 10.71YY125 pKa = 10.54DD126 pKa = 3.71FADD129 pKa = 4.13ADD131 pKa = 4.22SVLKK135 pKa = 10.34ILPEE139 pKa = 3.86WPEE142 pKa = 3.52EE143 pKa = 3.8LVPQKK148 pKa = 10.39LSSMSGFLVFMGLVSDD164 pKa = 4.13GTFANQLIVWSDD176 pKa = 3.18AAEE179 pKa = 4.45LGSLPDD185 pKa = 3.53NYY187 pKa = 10.99NWADD191 pKa = 3.42PTSRR195 pKa = 11.84AGFYY199 pKa = 10.48SLPDD203 pKa = 3.49FEE205 pKa = 5.24EE206 pKa = 4.45FVAAVLLNKK215 pKa = 10.29SLIIYY220 pKa = 7.46RR221 pKa = 11.84TNSIYY226 pKa = 10.64EE227 pKa = 3.66MRR229 pKa = 11.84FIGGTLVFSIEE240 pKa = 3.69QRR242 pKa = 11.84FEE244 pKa = 3.87GKK246 pKa = 7.95TLISSKK252 pKa = 10.75AVAAKK257 pKa = 10.38GRR259 pKa = 11.84LHH261 pKa = 5.94YY262 pKa = 10.71CIGEE266 pKa = 4.08NEE268 pKa = 4.6FYY270 pKa = 11.41VHH272 pKa = 7.34DD273 pKa = 4.81GSSEE277 pKa = 3.96QVFDD281 pKa = 3.66VRR283 pKa = 11.84PVSDD287 pKa = 4.08YY288 pKa = 11.53YY289 pKa = 11.57FNTVNPDD296 pKa = 3.29YY297 pKa = 11.02AYY299 pKa = 8.86LTQMVYY305 pKa = 10.58DD306 pKa = 3.79QSQQRR311 pKa = 11.84LHH313 pKa = 5.98ICYY316 pKa = 9.75PSGNDD321 pKa = 3.57TLCTRR326 pKa = 11.84DD327 pKa = 3.54LFFDD331 pKa = 4.82FKK333 pKa = 11.12SNCWSLEE340 pKa = 3.67LLDD343 pKa = 4.65GYY345 pKa = 10.97SYY347 pKa = 10.89IGDD350 pKa = 3.66VFISSTPQLIYY361 pKa = 10.79EE362 pKa = 4.45DD363 pKa = 4.52ANMTFEE369 pKa = 6.26DD370 pKa = 3.9STDD373 pKa = 3.77DD374 pKa = 4.01FNLVYY379 pKa = 10.85DD380 pKa = 3.92KK381 pKa = 11.05FSNNRR386 pKa = 11.84LVYY389 pKa = 9.83FNGSIFQVGGEE400 pKa = 4.33SQVKK404 pKa = 9.31QATLTRR410 pKa = 11.84NLVAYY415 pKa = 6.56TTQDD419 pKa = 2.85TSGTTTVKK427 pKa = 10.64RR428 pKa = 11.84NLQLLITEE436 pKa = 5.16LWPKK440 pKa = 10.31LYY442 pKa = 10.54SGQVQFRR449 pKa = 11.84LGFSEE454 pKa = 4.69TPLGTITWSDD464 pKa = 3.08WVSTNGLDD472 pKa = 4.04RR473 pKa = 11.84LDD475 pKa = 4.21FFLSGLYY482 pKa = 10.25LHH484 pKa = 6.64LQIRR488 pKa = 11.84NEE490 pKa = 4.19GQDD493 pKa = 3.44FTLNGYY499 pKa = 7.56MLQAAPTGKK508 pKa = 9.8IAA510 pKa = 4.71

Molecular weight: 57.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WBI9|A0A5Q2WBI9_9CAUD Uncharacterized protein OS=Siphoviridae sp. ct7UA22 OX=2656718 PE=4 SV=1
MM1 pKa = 6.99VRR3 pKa = 11.84SFHH6 pKa = 6.98ALRR9 pKa = 11.84FSAWQRR15 pKa = 11.84EE16 pKa = 4.24KK17 pKa = 10.86PHH19 pKa = 6.89DD20 pKa = 4.66LGWYY24 pKa = 9.45IKK26 pKa = 10.43GAYY29 pKa = 9.16HH30 pKa = 5.42GQKK33 pKa = 9.7RR34 pKa = 11.84HH35 pKa = 4.86

Molecular weight: 4.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13272

29

1890

241.3

26.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.546 ± 0.514

1.07 ± 0.19

5.998 ± 0.233

6.005 ± 0.258

4.28 ± 0.238

6.299 ± 0.339

1.756 ± 0.182

4.83 ± 0.25

5.432 ± 0.327

9.411 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.171

4.204 ± 0.201

4.913 ± 0.258

4.671 ± 0.403

5.206 ± 0.266

6.201 ± 0.319

6.065 ± 0.33

6.608 ± 0.314

1.386 ± 0.105

3.308 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski