Amino acid dipepetide frequency for Siphoviridae sp. ct7UA22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.268AlaAla: 9.268 ± 1.459
0.754AlaCys: 0.754 ± 0.253
5.199AlaAsp: 5.199 ± 0.625
5.877AlaGlu: 5.877 ± 0.755
3.391AlaPhe: 3.391 ± 0.473
6.631AlaGly: 6.631 ± 0.794
2.185AlaHis: 2.185 ± 0.326
4.898AlaIle: 4.898 ± 0.677
4.672AlaLys: 4.672 ± 0.516
9.946AlaLeu: 9.946 ± 0.757
3.014AlaMet: 3.014 ± 0.458
3.692AlaAsn: 3.692 ± 0.704
6.179AlaPro: 6.179 ± 0.902
5.049AlaGln: 5.049 ± 1.194
5.124AlaArg: 5.124 ± 0.618
4.596AlaSer: 4.596 ± 0.752
5.124AlaThr: 5.124 ± 0.569
5.953AlaVal: 5.953 ± 0.584
1.055AlaTrp: 1.055 ± 0.221
2.863AlaTyr: 2.863 ± 0.389
0.0AlaXaa: 0.0 ± 0.0
Cys
0.754CysAla: 0.754 ± 0.281
0.603CysCys: 0.603 ± 0.323
0.377CysAsp: 0.377 ± 0.175
0.527CysGlu: 0.527 ± 0.222
0.754CysPhe: 0.754 ± 0.29
0.754CysGly: 0.754 ± 0.25
0.226CysHis: 0.226 ± 0.121
1.055CysIle: 1.055 ± 0.289
0.754CysLys: 0.754 ± 0.258
0.301CysLeu: 0.301 ± 0.148
0.226CysMet: 0.226 ± 0.139
0.301CysAsn: 0.301 ± 0.141
0.377CysPro: 0.377 ± 0.161
0.527CysGln: 0.527 ± 0.191
0.829CysArg: 0.829 ± 0.307
0.678CysSer: 0.678 ± 0.228
0.377CysThr: 0.377 ± 0.162
0.678CysVal: 0.678 ± 0.217
0.226CysTrp: 0.226 ± 0.122
0.377CysTyr: 0.377 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
4.37AspAla: 4.37 ± 0.422
0.904AspCys: 0.904 ± 0.307
3.014AspAsp: 3.014 ± 0.567
3.391AspGlu: 3.391 ± 0.533
4.823AspPhe: 4.823 ± 0.656
3.918AspGly: 3.918 ± 0.555
0.829AspHis: 0.829 ± 0.334
3.692AspIle: 3.692 ± 0.575
3.014AspLys: 3.014 ± 0.459
6.48AspLeu: 6.48 ± 0.7
1.959AspMet: 1.959 ± 0.371
2.411AspAsn: 2.411 ± 0.475
3.165AspPro: 3.165 ± 0.614
1.432AspGln: 1.432 ± 0.277
2.411AspArg: 2.411 ± 0.479
2.713AspSer: 2.713 ± 0.534
4.446AspThr: 4.446 ± 0.549
4.144AspVal: 4.144 ± 0.498
0.904AspTrp: 0.904 ± 0.244
1.884AspTyr: 1.884 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
5.877GluAla: 5.877 ± 0.75
0.603GluCys: 0.603 ± 0.271
3.994GluAsp: 3.994 ± 0.461
3.768GluGlu: 3.768 ± 0.74
3.165GluPhe: 3.165 ± 0.537
3.542GluGly: 3.542 ± 0.445
1.055GluHis: 1.055 ± 0.302
2.637GluIle: 2.637 ± 0.569
3.994GluLys: 3.994 ± 0.796
6.782GluLeu: 6.782 ± 0.626
1.658GluMet: 1.658 ± 0.297
1.507GluAsn: 1.507 ± 0.335
1.733GluPro: 1.733 ± 0.392
3.165GluGln: 3.165 ± 0.434
2.939GluArg: 2.939 ± 0.502
3.391GluSer: 3.391 ± 0.314
3.843GluThr: 3.843 ± 0.492
3.994GluVal: 3.994 ± 0.799
1.206GluTrp: 1.206 ± 0.235
1.206GluTyr: 1.206 ± 0.225
0.0GluXaa: 0.0 ± 0.0
Phe
3.466PheAla: 3.466 ± 0.597
0.452PheCys: 0.452 ± 0.184
3.617PheAsp: 3.617 ± 0.35
2.637PheGlu: 2.637 ± 0.355
2.185PhePhe: 2.185 ± 0.364
3.692PheGly: 3.692 ± 0.593
1.13PheHis: 1.13 ± 0.244
2.035PheIle: 2.035 ± 0.488
1.206PheLys: 1.206 ± 0.359
4.295PheLeu: 4.295 ± 0.639
0.904PheMet: 0.904 ± 0.219
2.562PheAsn: 2.562 ± 0.478
1.733PhePro: 1.733 ± 0.309
1.959PheGln: 1.959 ± 0.277
2.487PheArg: 2.487 ± 0.363
1.884PheSer: 1.884 ± 0.429
2.261PheThr: 2.261 ± 0.432
1.733PheVal: 1.733 ± 0.42
0.452PheTrp: 0.452 ± 0.19
2.11PheTyr: 2.11 ± 0.428
0.0PheXaa: 0.0 ± 0.0
Gly
5.877GlyAla: 5.877 ± 0.649
0.452GlyCys: 0.452 ± 0.198
2.562GlyAsp: 2.562 ± 0.399
3.542GlyGlu: 3.542 ± 0.483
3.24GlyPhe: 3.24 ± 0.506
4.295GlyGly: 4.295 ± 0.717
1.13GlyHis: 1.13 ± 0.255
3.24GlyIle: 3.24 ± 0.348
3.768GlyLys: 3.768 ± 0.554
7.46GlyLeu: 7.46 ± 0.851
2.035GlyMet: 2.035 ± 0.39
3.165GlyAsn: 3.165 ± 0.552
1.808GlyPro: 1.808 ± 0.349
2.788GlyGln: 2.788 ± 0.329
2.562GlyArg: 2.562 ± 0.443
3.843GlySer: 3.843 ± 0.687
3.768GlyThr: 3.768 ± 0.767
4.069GlyVal: 4.069 ± 0.561
0.754GlyTrp: 0.754 ± 0.286
2.637GlyTyr: 2.637 ± 0.47
0.0GlyXaa: 0.0 ± 0.0
His
1.356HisAla: 1.356 ± 0.3
0.151HisCys: 0.151 ± 0.099
1.206HisAsp: 1.206 ± 0.284
1.13HisGlu: 1.13 ± 0.261
0.829HisPhe: 0.829 ± 0.233
0.904HisGly: 0.904 ± 0.341
0.075HisHis: 0.075 ± 0.065
0.377HisIle: 0.377 ± 0.143
0.829HisLys: 0.829 ± 0.291
2.487HisLeu: 2.487 ± 0.414
0.377HisMet: 0.377 ± 0.111
0.904HisAsn: 0.904 ± 0.306
1.356HisPro: 1.356 ± 0.352
0.452HisGln: 0.452 ± 0.191
0.98HisArg: 0.98 ± 0.317
1.206HisSer: 1.206 ± 0.29
0.98HisThr: 0.98 ± 0.349
0.754HisVal: 0.754 ± 0.175
0.226HisTrp: 0.226 ± 0.13
0.98HisTyr: 0.98 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
5.651IleAla: 5.651 ± 0.556
0.904IleCys: 0.904 ± 0.27
3.24IleAsp: 3.24 ± 0.554
2.562IleGlu: 2.562 ± 0.397
1.281IlePhe: 1.281 ± 0.277
2.411IleGly: 2.411 ± 0.427
0.452IleHis: 0.452 ± 0.165
1.582IleIle: 1.582 ± 0.325
2.185IleLys: 2.185 ± 0.474
3.994IleLeu: 3.994 ± 0.443
0.829IleMet: 0.829 ± 0.214
2.261IleAsn: 2.261 ± 0.411
3.315IlePro: 3.315 ± 0.515
2.11IleGln: 2.11 ± 0.439
2.713IleArg: 2.713 ± 0.411
3.089IleSer: 3.089 ± 0.378
3.165IleThr: 3.165 ± 0.487
4.295IleVal: 4.295 ± 0.613
0.452IleTrp: 0.452 ± 0.187
1.808IleTyr: 1.808 ± 0.439
0.0IleXaa: 0.0 ± 0.0
Lys
5.501LysAla: 5.501 ± 0.868
0.603LysCys: 0.603 ± 0.203
3.542LysAsp: 3.542 ± 0.528
2.713LysGlu: 2.713 ± 0.397
1.507LysPhe: 1.507 ± 0.239
3.315LysGly: 3.315 ± 0.433
0.904LysHis: 0.904 ± 0.247
2.11LysIle: 2.11 ± 0.334
2.863LysLys: 2.863 ± 0.52
4.521LysLeu: 4.521 ± 0.549
1.507LysMet: 1.507 ± 0.345
2.035LysAsn: 2.035 ± 0.397
3.542LysPro: 3.542 ± 0.678
2.863LysGln: 2.863 ± 0.551
3.089LysArg: 3.089 ± 0.428
3.843LysSer: 3.843 ± 0.457
3.918LysThr: 3.918 ± 0.5
4.144LysVal: 4.144 ± 0.593
0.98LysTrp: 0.98 ± 0.243
0.829LysTyr: 0.829 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
10.097LeuAla: 10.097 ± 1.07
1.507LeuCys: 1.507 ± 0.411
7.234LeuAsp: 7.234 ± 0.89
4.747LeuGlu: 4.747 ± 0.653
3.692LeuPhe: 3.692 ± 0.546
6.631LeuGly: 6.631 ± 1.034
2.487LeuHis: 2.487 ± 0.703
3.768LeuIle: 3.768 ± 0.591
4.747LeuLys: 4.747 ± 0.886
7.083LeuLeu: 7.083 ± 0.789
2.261LeuMet: 2.261 ± 0.411
3.994LeuAsn: 3.994 ± 0.988
4.596LeuPro: 4.596 ± 0.606
3.843LeuGln: 3.843 ± 0.554
6.104LeuArg: 6.104 ± 0.746
7.912LeuSer: 7.912 ± 0.712
4.37LeuThr: 4.37 ± 0.513
4.596LeuVal: 4.596 ± 0.704
1.206LeuTrp: 1.206 ± 0.236
3.24LeuTyr: 3.24 ± 0.523
0.0LeuXaa: 0.0 ± 0.0
Met
2.939MetAla: 2.939 ± 0.471
0.301MetCys: 0.301 ± 0.164
1.432MetAsp: 1.432 ± 0.28
1.055MetGlu: 1.055 ± 0.287
1.206MetPhe: 1.206 ± 0.344
1.356MetGly: 1.356 ± 0.412
0.377MetHis: 0.377 ± 0.157
0.904MetIle: 0.904 ± 0.247
2.637MetLys: 2.637 ± 0.448
2.713MetLeu: 2.713 ± 0.374
0.377MetMet: 0.377 ± 0.169
1.13MetAsn: 1.13 ± 0.269
1.055MetPro: 1.055 ± 0.36
1.658MetGln: 1.658 ± 0.382
1.582MetArg: 1.582 ± 0.28
3.165MetSer: 3.165 ± 0.607
1.582MetThr: 1.582 ± 0.385
1.658MetVal: 1.658 ± 0.391
0.301MetTrp: 0.301 ± 0.148
0.678MetTyr: 0.678 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
4.069AsnAla: 4.069 ± 0.612
0.377AsnCys: 0.377 ± 0.179
1.884AsnAsp: 1.884 ± 0.402
2.637AsnGlu: 2.637 ± 0.404
1.507AsnPhe: 1.507 ± 0.299
2.863AsnGly: 2.863 ± 0.69
0.377AsnHis: 0.377 ± 0.154
2.487AsnIle: 2.487 ± 0.409
2.336AsnLys: 2.336 ± 0.453
4.446AsnLeu: 4.446 ± 0.623
1.281AsnMet: 1.281 ± 0.264
1.808AsnAsn: 1.808 ± 0.349
2.713AsnPro: 2.713 ± 0.468
2.035AsnGln: 2.035 ± 0.383
2.035AsnArg: 2.035 ± 0.361
2.261AsnSer: 2.261 ± 0.475
2.185AsnThr: 2.185 ± 0.37
2.487AsnVal: 2.487 ± 0.463
0.829AsnTrp: 0.829 ± 0.225
1.432AsnTyr: 1.432 ± 0.268
0.0AsnXaa: 0.0 ± 0.0
Pro
4.898ProAla: 4.898 ± 0.905
0.226ProCys: 0.226 ± 0.153
3.165ProAsp: 3.165 ± 0.451
4.823ProGlu: 4.823 ± 0.9
1.356ProPhe: 1.356 ± 0.236
1.432ProGly: 1.432 ± 0.255
0.603ProHis: 0.603 ± 0.218
1.658ProIle: 1.658 ± 0.357
3.089ProLys: 3.089 ± 0.527
4.22ProLeu: 4.22 ± 0.581
1.206ProMet: 1.206 ± 0.24
2.185ProAsn: 2.185 ± 0.318
1.281ProPro: 1.281 ± 0.353
3.843ProGln: 3.843 ± 0.896
1.884ProArg: 1.884 ± 0.343
3.24ProSer: 3.24 ± 0.461
3.843ProThr: 3.843 ± 0.57
4.672ProVal: 4.672 ± 0.779
0.301ProTrp: 0.301 ± 0.113
1.206ProTyr: 1.206 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
4.973GlnAla: 4.973 ± 1.169
0.226GlnCys: 0.226 ± 0.118
2.487GlnAsp: 2.487 ± 0.388
3.165GlnGlu: 3.165 ± 0.69
1.507GlnPhe: 1.507 ± 0.328
3.089GlnGly: 3.089 ± 0.484
0.452GlnHis: 0.452 ± 0.149
2.411GlnIle: 2.411 ± 0.3
1.507GlnLys: 1.507 ± 0.279
3.692GlnLeu: 3.692 ± 0.613
1.658GlnMet: 1.658 ± 0.377
2.411GlnAsn: 2.411 ± 0.454
1.959GlnPro: 1.959 ± 0.54
3.24GlnGln: 3.24 ± 0.849
2.637GlnArg: 2.637 ± 0.398
3.24GlnSer: 3.24 ± 0.691
3.089GlnThr: 3.089 ± 0.505
2.336GlnVal: 2.336 ± 0.436
0.527GlnTrp: 0.527 ± 0.249
2.11GlnTyr: 2.11 ± 0.402
0.0GlnXaa: 0.0 ± 0.0
Arg
3.994ArgAla: 3.994 ± 0.522
0.452ArgCys: 0.452 ± 0.207
2.637ArgAsp: 2.637 ± 0.39
3.014ArgGlu: 3.014 ± 0.557
1.808ArgPhe: 1.808 ± 0.301
3.391ArgGly: 3.391 ± 0.511
0.829ArgHis: 0.829 ± 0.22
3.24ArgIle: 3.24 ± 0.422
3.315ArgLys: 3.315 ± 0.584
5.425ArgLeu: 5.425 ± 0.931
1.356ArgMet: 1.356 ± 0.382
2.035ArgAsn: 2.035 ± 0.566
2.637ArgPro: 2.637 ± 0.424
1.959ArgGln: 1.959 ± 0.335
2.939ArgArg: 2.939 ± 0.423
3.089ArgSer: 3.089 ± 0.534
3.542ArgThr: 3.542 ± 0.583
3.617ArgVal: 3.617 ± 0.514
0.904ArgTrp: 0.904 ± 0.223
1.884ArgTyr: 1.884 ± 0.363
0.0ArgXaa: 0.0 ± 0.0
Ser
5.049SerAla: 5.049 ± 0.802
0.678SerCys: 0.678 ± 0.24
4.672SerAsp: 4.672 ± 0.521
3.843SerGlu: 3.843 ± 0.472
2.939SerPhe: 2.939 ± 0.519
4.069SerGly: 4.069 ± 0.803
1.13SerHis: 1.13 ± 0.24
3.014SerIle: 3.014 ± 0.463
3.165SerLys: 3.165 ± 0.467
5.651SerLeu: 5.651 ± 0.705
1.733SerMet: 1.733 ± 0.337
2.336SerAsn: 2.336 ± 0.482
2.788SerPro: 2.788 ± 0.354
2.336SerGln: 2.336 ± 0.486
3.315SerArg: 3.315 ± 0.399
3.617SerSer: 3.617 ± 0.568
3.014SerThr: 3.014 ± 0.532
4.973SerVal: 4.973 ± 0.667
1.432SerTrp: 1.432 ± 0.326
2.261SerTyr: 2.261 ± 0.42
0.0SerXaa: 0.0 ± 0.0
Thr
5.802ThrAla: 5.802 ± 0.697
0.527ThrCys: 0.527 ± 0.216
3.165ThrAsp: 3.165 ± 0.363
3.843ThrGlu: 3.843 ± 0.383
2.336ThrPhe: 2.336 ± 0.49
3.692ThrGly: 3.692 ± 0.682
0.98ThrHis: 0.98 ± 0.291
2.788ThrIle: 2.788 ± 0.37
4.37ThrLys: 4.37 ± 0.71
6.028ThrLeu: 6.028 ± 0.617
1.281ThrMet: 1.281 ± 0.326
2.035ThrAsn: 2.035 ± 0.414
3.768ThrPro: 3.768 ± 0.717
2.939ThrGln: 2.939 ± 0.461
2.788ThrArg: 2.788 ± 0.427
3.315ThrSer: 3.315 ± 0.612
4.747ThrThr: 4.747 ± 0.7
3.617ThrVal: 3.617 ± 0.583
0.829ThrTrp: 0.829 ± 0.185
1.808ThrTyr: 1.808 ± 0.44
0.0ThrXaa: 0.0 ± 0.0
Val
6.556ValAla: 6.556 ± 0.723
0.678ValCys: 0.678 ± 0.222
3.089ValAsp: 3.089 ± 0.52
3.617ValGlu: 3.617 ± 0.532
3.391ValPhe: 3.391 ± 0.523
3.692ValGly: 3.692 ± 0.577
1.206ValHis: 1.206 ± 0.372
4.069ValIle: 4.069 ± 0.679
3.542ValLys: 3.542 ± 0.583
4.898ValLeu: 4.898 ± 0.459
2.261ValMet: 2.261 ± 0.331
3.391ValAsn: 3.391 ± 0.482
3.089ValPro: 3.089 ± 0.43
2.713ValGln: 2.713 ± 0.484
3.466ValArg: 3.466 ± 0.487
3.768ValSer: 3.768 ± 0.539
4.521ValThr: 4.521 ± 0.747
5.501ValVal: 5.501 ± 0.669
0.603ValTrp: 0.603 ± 0.177
2.035ValTyr: 2.035 ± 0.391
0.0ValXaa: 0.0 ± 0.0
Trp
1.432TrpAla: 1.432 ± 0.304
0.0TrpCys: 0.0 ± 0.0
1.206TrpAsp: 1.206 ± 0.367
1.281TrpGlu: 1.281 ± 0.276
0.904TrpPhe: 0.904 ± 0.25
0.301TrpGly: 0.301 ± 0.126
0.452TrpHis: 0.452 ± 0.202
0.377TrpIle: 0.377 ± 0.155
0.754TrpLys: 0.754 ± 0.294
1.055TrpLeu: 1.055 ± 0.222
0.377TrpMet: 0.377 ± 0.157
0.301TrpAsn: 0.301 ± 0.134
0.527TrpPro: 0.527 ± 0.215
0.829TrpGln: 0.829 ± 0.207
0.829TrpArg: 0.829 ± 0.252
1.206TrpSer: 1.206 ± 0.327
0.377TrpThr: 0.377 ± 0.15
0.829TrpVal: 0.829 ± 0.234
0.301TrpTrp: 0.301 ± 0.161
0.527TrpTyr: 0.527 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.542TyrAla: 3.542 ± 0.372
0.301TyrCys: 0.301 ± 0.15
2.261TyrAsp: 2.261 ± 0.427
1.884TyrGlu: 1.884 ± 0.318
0.98TyrPhe: 0.98 ± 0.239
3.014TyrGly: 3.014 ± 0.494
0.678TyrHis: 0.678 ± 0.188
1.959TyrIle: 1.959 ± 0.344
1.582TyrLys: 1.582 ± 0.327
2.637TyrLeu: 2.637 ± 0.487
1.733TyrMet: 1.733 ± 0.35
1.582TyrAsn: 1.582 ± 0.39
1.507TyrPro: 1.507 ± 0.337
0.98TyrGln: 0.98 ± 0.314
1.356TyrArg: 1.356 ± 0.28
1.959TyrSer: 1.959 ± 0.4
1.507TyrThr: 1.507 ± 0.339
2.035TyrVal: 2.035 ± 0.397
0.377TyrTrp: 0.377 ± 0.203
1.206TyrTyr: 1.206 ± 0.327
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13272 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski