Bovine respiratory syncytial virus (strain A51908) (BRS)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus; Bovine orthopneumovirus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P62648-2|GLYC-2_BRSVA Isoform of P62648 Isoform Secreted glycoprotein G of Major surface glycoprotein G OS=Bovine respiratory syncytial virus (strain A51908) OX=11247 GN=G PE=1 SV=1
MM1 pKa = 7.39NNSNIIIFPEE11 pKa = 4.59KK12 pKa = 9.62YY13 pKa = 8.38PCSISSLLIKK23 pKa = 10.5DD24 pKa = 3.88EE25 pKa = 4.14NDD27 pKa = 3.36VFVLSHH33 pKa = 6.36QNVLDD38 pKa = 4.04CLQFQYY44 pKa = 10.28PYY46 pKa = 11.7NMYY49 pKa = 10.05SQNHH53 pKa = 5.47MLDD56 pKa = 4.25DD57 pKa = 4.7IYY59 pKa = 9.89WTSQEE64 pKa = 4.6LIEE67 pKa = 4.95DD68 pKa = 3.77VLKK71 pKa = 10.59ILHH74 pKa = 6.63LSGISINKK82 pKa = 7.87YY83 pKa = 8.85VIYY86 pKa = 10.84VLVLL90 pKa = 3.28

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q65694|NS1_BRSVA Non-structural protein 1 OS=Bovine respiratory syncytial virus (strain A51908) OX=11247 GN=1C PE=2 SV=1
MM1 pKa = 7.56SNHH4 pKa = 4.32THH6 pKa = 5.99HH7 pKa = 7.36PKK9 pKa = 10.56FKK11 pKa = 8.79TLKK14 pKa = 9.66RR15 pKa = 11.84AWKK18 pKa = 8.2ASKK21 pKa = 10.57YY22 pKa = 10.19FIVGLSCLYY31 pKa = 10.63KK32 pKa = 10.77FNLKK36 pKa = 10.52SLVQTALTSLAMITLTSLVITAIIYY61 pKa = 10.23ISVGNAKK68 pKa = 10.16AKK70 pKa = 7.59PTSKK74 pKa = 9.21PTTQQTQQPQNHH86 pKa = 6.19TPLLPTEE93 pKa = 4.81HH94 pKa = 6.05NHH96 pKa = 6.54KK97 pKa = 9.28STHH100 pKa = 5.95TSTQSTTLSQPPNIDD115 pKa = 3.38TTSGTTYY122 pKa = 9.98GHH124 pKa = 7.56PINRR128 pKa = 11.84TQNRR132 pKa = 11.84KK133 pKa = 9.07IKK135 pKa = 10.34SQSTPLATRR144 pKa = 11.84KK145 pKa = 10.21LPINPLEE152 pKa = 4.39SNPPEE157 pKa = 3.73NHH159 pKa = 5.94QDD161 pKa = 3.42HH162 pKa = 6.87NNSQTLPHH170 pKa = 6.35VPCSTCEE177 pKa = 3.91GNPACSPLCQIGLEE191 pKa = 4.29RR192 pKa = 11.84APSRR196 pKa = 11.84APTITLKK203 pKa = 10.68KK204 pKa = 9.96APKK207 pKa = 9.76PKK209 pKa = 8.31TTKK212 pKa = 10.46KK213 pKa = 8.12PTKK216 pKa = 6.53TTIYY220 pKa = 10.39HH221 pKa = 5.41RR222 pKa = 11.84TSPEE226 pKa = 3.5AKK228 pKa = 10.14LQTKK232 pKa = 10.23KK233 pKa = 9.31NTATPQQGILSSPEE247 pKa = 3.74HH248 pKa = 5.42QTNQSTTQISQHH260 pKa = 4.92TSII263 pKa = 5.06

Molecular weight: 28.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

1

12

4710

73

2162

392.5

44.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.268 ± 0.663

1.847 ± 0.258

4.756 ± 0.476

4.968 ± 0.742

3.397 ± 0.449

4.034 ± 0.504

2.76 ± 0.387

8.896 ± 0.488

7.558 ± 0.275

10.849 ± 1.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.269

7.197 ± 0.285

4.013 ± 0.718

3.461 ± 0.515

3.652 ± 0.256

8.174 ± 0.553

7.877 ± 1.146

5.074 ± 0.685

0.786 ± 0.224

4.183 ± 0.572
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski