Mucinivorans hirudinis
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2816 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060RDV4|A0A060RDV4_9BACT BACON domain-containing protein OS=Mucinivorans hirudinis OX=1433126 GN=BN938_2076 PE=4 SV=1
MM1 pKa = 7.21 YY2 pKa = 8.93 EE3 pKa = 4.31 AYY5 pKa = 10.57 YY6 pKa = 11.31 ADD8 pKa = 3.92 AEE10 pKa = 4.24 RR11 pKa = 11.84 LAVLSGAKK19 pKa = 8.59 STIVDD24 pKa = 4.14 EE25 pKa = 4.6 VPTFYY30 pKa = 11.13 VAFEE34 pKa = 5.13 DD35 pKa = 4.45 ISVLMDD41 pKa = 3.98 RR42 pKa = 11.84 LSKK45 pKa = 11.03 ADD47 pKa = 3.39 VAVCISEE54 pKa = 4.22 MQDD57 pKa = 2.63 SDD59 pKa = 4.28 GNFVPTINYY68 pKa = 9.65 EE69 pKa = 4.03 EE70 pKa = 4.37 EE71 pKa = 3.97
Molecular weight: 7.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.935
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A060R6D5|A0A060R6D5_9BACT HNHc domain-containing protein OS=Mucinivorans hirudinis OX=1433126 GN=BN938_0387 PE=4 SV=1
MM1 pKa = 7.11 FTTTLSILKK10 pKa = 8.63 TAFRR14 pKa = 11.84 EE15 pKa = 4.47 SINCSRR21 pKa = 11.84 NGYY24 pKa = 9.8 QMANKK29 pKa = 8.89 KK30 pKa = 10.18 RR31 pKa = 11.84 KK32 pKa = 9.15 IGRR35 pKa = 11.84 FSTWRR40 pKa = 11.84 YY41 pKa = 8.01 LALVGVTWLPYY52 pKa = 9.89 RR53 pKa = 11.84 CGSDD57 pKa = 2.77 RR58 pKa = 4.07
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.984
IPC_protein 10.833
Toseland 10.804
ProMoST 10.994
Dawson 10.906
Bjellqvist 10.657
Wikipedia 11.14
Rodwell 11.125
Grimsley 10.965
Solomon 11.038
Lehninger 11.008
Nozaki 10.804
DTASelect 10.643
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.847
Patrickios 10.906
IPC_peptide 11.052
IPC2_peptide 9.853
IPC2.peptide.svr19 8.399
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2816
0
2816
879195
37
2204
312.2
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.552 ± 0.047
1.118 ± 0.017
5.358 ± 0.031
6.567 ± 0.043
4.597 ± 0.029
6.798 ± 0.04
1.676 ± 0.02
7.441 ± 0.038
6.4 ± 0.045
8.903 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.584 ± 0.019
5.038 ± 0.04
3.508 ± 0.023
3.439 ± 0.027
5.129 ± 0.032
6.344 ± 0.036
5.497 ± 0.03
6.783 ± 0.036
1.16 ± 0.017
4.106 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here