Labrenzia sp. OB1
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5016 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161YYJ8|A0A161YYJ8_9RHOB Uncharacterized protein OS=Labrenzia sp. OB1 OX=1561204 GN=OA90_15465 PE=4 SV=1
MM1 pKa = 7.33 QSTGAQGQDD10 pKa = 2.53 TGTAGNGGEE19 pKa = 3.56 IRR21 pKa = 11.84 IYY23 pKa = 9.9 NDD25 pKa = 2.79 SYY27 pKa = 11.64 INYY30 pKa = 9.09 QVGNTSSLAVGMNARR45 pKa = 11.84 SLGGAGDD52 pKa = 3.62 QDD54 pKa = 4.57 NRR56 pKa = 11.84 DD57 pKa = 3.48 NDD59 pKa = 4.42 SNGGTGGNGGSLYY72 pKa = 9.39 FTSDD76 pKa = 3.62 GIAGYY81 pKa = 10.4 DD82 pKa = 3.28 VDD84 pKa = 4.43 GGSNAGFVIYY94 pKa = 10.47 LGEE97 pKa = 4.33 SYY99 pKa = 11.26 GGMGGKK105 pKa = 9.87 QNDD108 pKa = 4.1 SVLSDD113 pKa = 3.23 QRR115 pKa = 11.84 GGQGGSGGAITIVNNSLASFDD136 pKa = 3.9 IGSSSSRR143 pKa = 11.84 FTGMGSGGGFFAKK156 pKa = 10.24 SVGGQGGFNNGNAGAGGAVSITGDD180 pKa = 3.95 LDD182 pKa = 3.66 GSIYY186 pKa = 10.27 WDD188 pKa = 3.49 VTAGGGTGLFGLSGLSQGGDD208 pKa = 3.17 GTTSTDD214 pKa = 3.14 NSDD217 pKa = 3.28 NGGNGGNGGTVTISNGEE234 pKa = 4.07 GLVLDD239 pKa = 3.79 VLGTVGEE246 pKa = 4.38 ISAVLAGKK254 pKa = 9.72 SAGGNGGTGPAEE266 pKa = 3.84 DD267 pKa = 4.71 HH268 pKa = 6.65 YY269 pKa = 11.19 GGSGGTAGAVSLSLNNDD286 pKa = 2.71 GDD288 pKa = 4.18 LAASGDD294 pKa = 4.08 YY295 pKa = 10.9 VYY297 pKa = 11.14 GILGQSIGGEE307 pKa = 4.21 GGDD310 pKa = 4.34 GGDD313 pKa = 3.64 GTALAGTGGGGGFGGNAGNVTVSVDD338 pKa = 2.49 IWAGSVSTNGDD349 pKa = 3.52 YY350 pKa = 11.3 APAIAAHH357 pKa = 6.36 SIGGGGGTGGDD368 pKa = 3.7 FVSVLGGQAGNGGNGGNAGNVLVSLDD394 pKa = 3.77 AQGLTTSGDD403 pKa = 3.26 HH404 pKa = 6.49 SFGIVAQSIAGSGGTGGIDD423 pKa = 3.04 TSTLVGLGGDD433 pKa = 3.91 GAGGGSVGTVTINSSTPITTQGFNALGIIGQSIGGGGGAAGSASGLVSVGGNAQGSSTSDD493 pKa = 2.84 GGTVGISNTEE503 pKa = 3.98 SVVTKK508 pKa = 10.31 GEE510 pKa = 3.92 SSIAILAQSIGGGGGSGGDD529 pKa = 3.21 ATGIAGVGGQGAAGGDD545 pKa = 3.6 GGIIAIPNIGTITTYY560 pKa = 11.27 DD561 pKa = 3.65 DD562 pKa = 3.88 SSPGAVFQSIGGGGGNGGSTLTLSTIASIGIGGSASGGGDD602 pKa = 3.24 GGLLCIDD609 pKa = 3.96 NSAVCGANNSSGTFVPYY626 pKa = 10.63 LSSLASPEE634 pKa = 4.14 DD635 pKa = 4.1 DD636 pKa = 3.07 GDD638 pKa = 4.1 ARR640 pKa = 11.84 VTTIGDD646 pKa = 4.15 FAPGVVAQSVGGGGGNGGSDD666 pKa = 3.45 KK667 pKa = 11.08 NYY669 pKa = 10.06 SALSFFALQMGGSAGAGGAGGTVRR693 pKa = 11.84 FVQDD697 pKa = 3.16 EE698 pKa = 4.46 FAVNTSGAQSPGLIAQSIGGGGGTGGSASYY728 pKa = 11.25 YY729 pKa = 10.41 DD730 pKa = 3.41 ATIGFNAAFIVGGSGGAGGSGGTLNLDD757 pKa = 3.28 LTDD760 pKa = 4.6 AYY762 pKa = 9.92 IATGLITNSTTQSAPNNSVGILAQSIGGGGGSGGAVSASDD802 pKa = 3.52 FVVAAPTGTGVPVAFNYY819 pKa = 9.08 QAAVGGNGANAGNGGSVTVSFDD841 pKa = 3.37 NDD843 pKa = 3.41 SGLTTIGDD851 pKa = 3.84 AAHH854 pKa = 7.04 GIVAQSIGGGGGNGGDD870 pKa = 3.41 ASVLSTTLGDD880 pKa = 3.51 KK881 pKa = 10.2 DD882 pKa = 4.11 TVEE885 pKa = 4.32 VTAGVAFGGGAGNIMSDD902 pKa = 3.83 FNPGYY907 pKa = 10.61 SYY909 pKa = 7.69 TTSGGGAGGSVSVTLGTYY927 pKa = 10.53 GEE929 pKa = 4.29 YY930 pKa = 10.67 TNASGTGATRR940 pKa = 11.84 LTTLAPDD947 pKa = 3.62 GTMLTVGDD955 pKa = 4.21 GAHH958 pKa = 6.48 GVLAQSIGGGGGNGGVGNSNAYY980 pKa = 9.37 AQGGVASLKK989 pKa = 10.69 ADD991 pKa = 3.21 ISLGGQGGSGGNGNSVTVHH1010 pKa = 5.87 NFAGYY1015 pKa = 7.59 TVQTQGSGARR1025 pKa = 11.84 GIVAQSIGGGGGNSQGGTLYY1045 pKa = 10.9 LAANAGGFSGRR1056 pKa = 11.84 LNVGVGATGGSGGEE1070 pKa = 4.13 GGSVYY1075 pKa = 10.52 VEE1077 pKa = 4.16 EE1078 pKa = 4.65 YY1079 pKa = 11.07 GVTEE1083 pKa = 4.3 TFGGDD1088 pKa = 2.87 ADD1090 pKa = 4.9 GIVLQSIGGGGGIGGSLGSDD1110 pKa = 3.07 ASSHH1114 pKa = 6.8 KK1115 pKa = 10.69 ILDD1118 pKa = 3.71 QIGKK1122 pKa = 9.41 IKK1124 pKa = 11.05 NNLSRR1129 pKa = 11.84 LTDD1132 pKa = 3.47 AGSSYY1137 pKa = 11.13 EE1138 pKa = 3.92 LTVDD1142 pKa = 3.58 VGGKK1146 pKa = 9.59 GGNGGDD1152 pKa = 3.64 GGTVKK1157 pKa = 10.58 YY1158 pKa = 9.89 YY1159 pKa = 9.7 QAGRR1163 pKa = 11.84 VATYY1167 pKa = 10.83 GDD1169 pKa = 3.43 WADD1172 pKa = 3.61 GVVLQSIGGGGGQGGSSVATGGDD1195 pKa = 3.09 VTANIEE1201 pKa = 4.18 VSVGGTGGSGGVGGEE1216 pKa = 3.66 AAIWVSGQGSNLVEE1230 pKa = 3.91 THH1232 pKa = 6.75 GYY1234 pKa = 8.56 GAAGLLMQSIGGGGGQGGDD1253 pKa = 3.68 GSDD1256 pKa = 3.59 SQTGTMAIGGDD1267 pKa = 3.25 WGGSGGAGNDD1277 pKa = 3.45 GGSVYY1282 pKa = 10.13 TYY1284 pKa = 10.17 NDD1286 pKa = 2.9 SDD1288 pKa = 3.99 IFINTYY1294 pKa = 10.47 GSDD1297 pKa = 3.53 AAGLIAQAIGGGGGTGGVGSSVEE1320 pKa = 3.77 ATRR1323 pKa = 11.84 ADD1325 pKa = 3.35 GHH1327 pKa = 5.74 EE1328 pKa = 4.34 LSVSVGGSGGVAGDD1342 pKa = 3.52 GGTINLQADD1351 pKa = 3.94 TSIVTLGNRR1360 pKa = 11.84 SHH1362 pKa = 6.57 GVLLQSIGGGGGLGVTGSVGDD1383 pKa = 4.71 DD1384 pKa = 3.16 LDD1386 pKa = 4.06 LAIGGTGGAAGDD1398 pKa = 4.48 GNTVTYY1404 pKa = 10.64 SLTGGTGVATGGIGAHH1420 pKa = 5.56 GVIAQSIGGGGGLGGDD1436 pKa = 3.39 ATGGVLSVLTSGDD1449 pKa = 3.56 NASGGASGNGGTITLDD1465 pKa = 2.76 IAAPIAVTGQDD1476 pKa = 3.39 AIGVVAQSIGGGGGLGGSGTGAFAGVTSSDD1506 pKa = 3.29 GSGTGGDD1513 pKa = 3.55 VTLTISDD1520 pKa = 4.08 KK1521 pKa = 10.96 VSSTGTRR1528 pKa = 11.84 GMGVFAQSQGPDD1540 pKa = 3.1 GNEE1543 pKa = 3.22 QVMITTNSGGQIIGGYY1559 pKa = 8.81 SDD1561 pKa = 3.68 SSSAVMVAGGNGNSLVLNGDD1581 pKa = 3.85 SVVEE1585 pKa = 4.17 SGSYY1589 pKa = 7.51 VTGNDD1594 pKa = 3.11 YY1595 pKa = 11.53 DD1596 pKa = 4.33 DD1597 pKa = 4.67 NYY1599 pKa = 11.19 AVRR1602 pKa = 11.84 YY1603 pKa = 9.17 SGSGTTAEE1611 pKa = 4.21 GAILDD1616 pKa = 3.85 VTVNDD1621 pKa = 3.99 TASLHH1626 pKa = 6.53 GDD1628 pKa = 3.51 VLLEE1632 pKa = 4.23 NADD1635 pKa = 3.64 GNTAGTVTNNSSNTLAGGYY1654 pKa = 9.81 FYY1656 pKa = 11.04 GAHH1659 pKa = 6.15 IVNNGRR1665 pKa = 11.84 FVLNRR1670 pKa = 11.84 EE1671 pKa = 4.06 HH1672 pKa = 7.35 DD1673 pKa = 3.45 TGTTVLTGNFIQSDD1687 pKa = 3.75 AGKK1690 pKa = 10.04 LVADD1694 pKa = 4.21 IDD1696 pKa = 4.43 LNNYY1700 pKa = 9.74 GSDD1703 pKa = 3.44 LFDD1706 pKa = 3.28 IRR1708 pKa = 11.84 GDD1710 pKa = 3.46 ASLDD1714 pKa = 3.3 GTLGVDD1720 pKa = 5.08 AISLLPSRR1728 pKa = 11.84 RR1729 pKa = 11.84 TRR1731 pKa = 11.84 FLTVGGTLSGSLTPQDD1747 pKa = 4.14 SLIFDD1752 pKa = 3.66 YY1753 pKa = 11.05 AINKK1757 pKa = 9.57 IGTDD1761 pKa = 3.04 HH1762 pKa = 6.22 YY1763 pKa = 11.22 LSVEE1767 pKa = 4.23 GADD1770 pKa = 3.59 FTPVDD1775 pKa = 3.9 KK1776 pKa = 10.1 TQMNDD1781 pKa = 3.14 DD1782 pKa = 4.17 QIRR1785 pKa = 11.84 LAKK1788 pKa = 10.14 NLQSIWNLGGNNAFGSLFGAIANEE1812 pKa = 3.75 VDD1814 pKa = 3.31 NAGRR1818 pKa = 11.84 DD1819 pKa = 3.63 YY1820 pKa = 11.71 GDD1822 pKa = 3.52 TLDD1825 pKa = 3.81 EE1826 pKa = 4.34 LRR1828 pKa = 11.84 PRR1830 pKa = 11.84 AALAPAVEE1838 pKa = 4.62 QSFEE1842 pKa = 3.83 MLAFGNSLMSCPVFEE1857 pKa = 5.57 ADD1859 pKa = 4.34 NAFLSEE1865 pKa = 4.92 GSCGWARR1872 pKa = 11.84 LTAATGHH1879 pKa = 5.6 RR1880 pKa = 11.84 QSMGTAPSYY1889 pKa = 10.93 NADD1892 pKa = 2.97 AVTYY1896 pKa = 10.33 TMGAQKK1902 pKa = 10.25 EE1903 pKa = 4.22 IAPGWFVGTAAAFQQSWISQASNSVSGEE1931 pKa = 4.18 GIGGFAGVSLKK1942 pKa = 10.92 HH1943 pKa = 6.57 EE1944 pKa = 4.55 IGDD1947 pKa = 3.71 WTLSGAVSGGYY1958 pKa = 8.95 GQYY1961 pKa = 11.18 DD1962 pKa = 4.02 LDD1964 pKa = 3.94 RR1965 pKa = 11.84 RR1966 pKa = 11.84 ISITNVSEE1974 pKa = 4.48 TVSSSPNVYY1983 pKa = 10.27 NGAIRR1988 pKa = 11.84 ARR1990 pKa = 11.84 IARR1993 pKa = 11.84 TFAATDD1999 pKa = 4.45 FYY2001 pKa = 11.58 AKK2003 pKa = 10.24 PYY2005 pKa = 10.08 VDD2007 pKa = 4.96 LDD2009 pKa = 3.57 AVYY2012 pKa = 11.0 SRR2014 pKa = 11.84 VGEE2017 pKa = 4.09 YY2018 pKa = 10.65 KK2019 pKa = 8.95 EE2020 pKa = 4.02 TGVYY2024 pKa = 10.46 GLEE2027 pKa = 4.66 FDD2029 pKa = 5.73 EE2030 pKa = 4.24 TDD2032 pKa = 2.71 QWNFAVSPSLEE2043 pKa = 4.07 LGSWYY2048 pKa = 9.61 TYY2050 pKa = 11.15 DD2051 pKa = 3.15 SGASLRR2057 pKa = 11.84 FFGRR2061 pKa = 11.84 VGLTVLAKK2069 pKa = 10.57 DD2070 pKa = 3.76 DD2071 pKa = 4.1 WQTSAQFTSAPAGTGKK2087 pKa = 8.61 FTTTLDD2093 pKa = 3.67 HH2094 pKa = 7.02 EE2095 pKa = 5.14 DD2096 pKa = 3.37 VFASIGAGLQLTSIDD2111 pKa = 3.95 GFDD2114 pKa = 4.79 LRR2116 pKa = 11.84 LEE2118 pKa = 3.96 YY2119 pKa = 10.54 DD2120 pKa = 3.39 GRR2122 pKa = 11.84 FSEE2125 pKa = 5.66 KK2126 pKa = 9.5 MATNSGSFRR2135 pKa = 11.84 VSIPFF2140 pKa = 3.65
Molecular weight: 205.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.821
IPC_protein 3.884
Toseland 3.643
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.846
Rodwell 3.706
Grimsley 3.554
Solomon 3.884
Lehninger 3.846
Nozaki 3.986
DTASelect 4.291
Thurlkill 3.694
EMBOSS 3.846
Sillero 4.012
Patrickios 1.71
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A163U730|A0A163U730_9RHOB Uncharacterized protein OS=Labrenzia sp. OB1 OX=1561204 GN=OA90_21190 PE=4 SV=1
MM1 pKa = 7.56 ALKK4 pKa = 10.08 KK5 pKa = 9.9 ICYY8 pKa = 9.65 RR9 pKa = 11.84 LEE11 pKa = 3.99 NRR13 pKa = 11.84 NIACEE18 pKa = 3.83 FGVFGKK24 pKa = 10.0 DD25 pKa = 3.01 CRR27 pKa = 11.84 QVAEE31 pKa = 4.2 KK32 pKa = 10.08 AQRR35 pKa = 11.84 RR36 pKa = 11.84 HH37 pKa = 4.2 RR38 pKa = 11.84 HH39 pKa = 5.64 RR40 pKa = 11.84 YY41 pKa = 7.74 GPSPIMDD48 pKa = 4.39 GNRR51 pKa = 11.84 EE52 pKa = 4.0 RR53 pKa = 11.84 APGDD57 pKa = 3.51 LAEE60 pKa = 4.63 KK61 pKa = 10.52 DD62 pKa = 3.33 IVRR65 pKa = 11.84 EE66 pKa = 3.63 RR67 pKa = 11.84 LIRR70 pKa = 11.84 LFVRR74 pKa = 11.84 RR75 pKa = 11.84 LEE77 pKa = 4.15 VRR79 pKa = 3.29
Molecular weight: 9.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.428
IPC_protein 10.072
Toseland 10.526
ProMoST 10.496
Dawson 10.628
Bjellqvist 10.335
Wikipedia 10.818
Rodwell 10.804
Grimsley 10.672
Solomon 10.745
Lehninger 10.716
Nozaki 10.555
DTASelect 10.321
Thurlkill 10.526
EMBOSS 10.935
Sillero 10.57
Patrickios 10.57
IPC_peptide 10.745
IPC2_peptide 9.545
IPC2.peptide.svr19 8.632
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5016
0
5016
1582622
33
4135
315.5
34.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.237 ± 0.044
0.893 ± 0.01
5.952 ± 0.032
6.101 ± 0.039
3.996 ± 0.025
8.495 ± 0.039
1.952 ± 0.018
5.452 ± 0.023
3.665 ± 0.026
10.213 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.523 ± 0.016
2.886 ± 0.02
4.844 ± 0.028
3.181 ± 0.021
6.345 ± 0.038
5.844 ± 0.027
5.477 ± 0.028
7.369 ± 0.024
1.276 ± 0.013
2.299 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here