Kocuria soli
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2527 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N3ZRM0|A0A3N3ZRM0_9MICC Uncharacterized protein OS=Kocuria soli OX=2485125 GN=EDL96_04070 PE=4 SV=1
MM1 pKa = 7.75 DD2 pKa = 5.29 HH3 pKa = 7.15 GSGGMAGHH11 pKa = 6.38 NPEE14 pKa = 5.04 GGPPPEE20 pKa = 5.34 GIEE23 pKa = 4.04 TAPEE27 pKa = 3.44 PTYY30 pKa = 10.55 PVDD33 pKa = 3.52 STVVLTADD41 pKa = 4.42 HH42 pKa = 6.44 MPGMAGSEE50 pKa = 4.03 ATITGAFDD58 pKa = 3.03 TTAYY62 pKa = 9.79 SVSYY66 pKa = 9.71 TPTDD70 pKa = 3.11 GGEE73 pKa = 3.88 PVVDD77 pKa = 4.16 HH78 pKa = 6.48 KK79 pKa = 10.95 WVVHH83 pKa = 6.32 EE84 pKa = 4.16 EE85 pKa = 4.2 LEE87 pKa = 4.74 DD88 pKa = 4.12 PGEE91 pKa = 4.36 APLPAGTEE99 pKa = 4.1 VVLNADD105 pKa = 4.44 HH106 pKa = 6.43 MPGMDD111 pKa = 3.35 GAEE114 pKa = 3.98 ATIEE118 pKa = 4.15 SSTQEE123 pKa = 3.85 TVYY126 pKa = 10.49 MVDD129 pKa = 3.23 TTIDD133 pKa = 3.39 GMEE136 pKa = 4.15 MTNHH140 pKa = 5.01 KK141 pKa = 9.85 WFVEE145 pKa = 4.26 SEE147 pKa = 4.22 LQPAEE152 pKa = 3.95
Molecular weight: 16.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.884
IPC_protein 3.834
Toseland 3.656
ProMoST 3.935
Dawson 3.795
Bjellqvist 3.986
Wikipedia 3.681
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.062
Thurlkill 3.694
EMBOSS 3.706
Sillero 3.948
Patrickios 0.985
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A3N3ZS15|A0A3N3ZS15_9MICC BCCT family transporter OS=Kocuria soli OX=2485125 GN=EDL96_07165 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 10.04 KK16 pKa = 8.86 HH17 pKa = 4.36 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.82 GRR40 pKa = 11.84 ASLSAA45 pKa = 3.83
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2527
0
2527
857169
32
1974
339.2
36.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.121 ± 0.055
0.644 ± 0.013
6.251 ± 0.045
6.234 ± 0.045
2.922 ± 0.032
8.916 ± 0.043
2.24 ± 0.022
3.93 ± 0.033
2.338 ± 0.033
9.814 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.074 ± 0.021
2.205 ± 0.024
5.496 ± 0.034
3.363 ± 0.027
7.119 ± 0.051
5.713 ± 0.032
6.187 ± 0.033
8.986 ± 0.044
1.521 ± 0.02
1.927 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here