Streptomyces sp. A0642

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6934 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2V1I5|A0A4S2V1I5_9ACTN Flavoprotein oxidoreductase OS=Streptomyces sp. A0642 OX=2563100 GN=E6R60_00210 PE=4 SV=1
MM1 pKa = 7.51SNEE4 pKa = 3.79DD5 pKa = 3.67HH6 pKa = 7.38ADD8 pKa = 3.15QHH10 pKa = 6.35GRR12 pKa = 11.84WEE14 pKa = 4.0PTPQGGEE21 pKa = 3.8YY22 pKa = 10.25DD23 pKa = 3.75AEE25 pKa = 4.03ATAFVHH31 pKa = 6.98LPPEE35 pKa = 4.33DD36 pKa = 3.69LANIPLAAPGQGYY49 pKa = 8.52VPPMILPLTPAAGLDD64 pKa = 3.66PAATGNWVVATPDD77 pKa = 3.36QPDD80 pKa = 3.54EE81 pKa = 4.19QEE83 pKa = 4.53PGAGPAAVHH92 pKa = 6.12WPDD95 pKa = 4.07PNQQQTSYY103 pKa = 10.52GYY105 pKa = 8.53PQASQDD111 pKa = 3.59YY112 pKa = 8.69PDD114 pKa = 5.6PYY116 pKa = 10.41TGTQAATGQWNFAEE130 pKa = 4.13NEE132 pKa = 4.45GPVEE136 pKa = 4.18AAGHH140 pKa = 5.79TGQWTIPVADD150 pKa = 3.97GEE152 pKa = 4.37LPEE155 pKa = 4.33EE156 pKa = 4.39SGEE159 pKa = 4.17FSASALAAGWYY170 pKa = 8.69QDD172 pKa = 3.46RR173 pKa = 11.84TPPATLPGGAPAPWATQEE191 pKa = 4.02PQPAATEE198 pKa = 4.31TPARR202 pKa = 11.84GTDD205 pKa = 3.72LGPLPEE211 pKa = 4.73SAAEE215 pKa = 4.35APAEE219 pKa = 4.31APAEE223 pKa = 4.33APAEE227 pKa = 4.31APAEE231 pKa = 4.17EE232 pKa = 4.99PGADD236 pKa = 3.31PVADD240 pKa = 3.94GTAEE244 pKa = 4.11AVPGTPDD251 pKa = 3.61DD252 pKa = 3.95LAHH255 pKa = 7.16RR256 pKa = 11.84SPAEE260 pKa = 3.66EE261 pKa = 4.17TAPDD265 pKa = 4.12TAPEE269 pKa = 4.38DD270 pKa = 4.08APEE273 pKa = 4.02QPEE276 pKa = 3.91ASAPDD281 pKa = 3.85GVVPDD286 pKa = 5.17DD287 pKa = 4.27AASDD291 pKa = 4.38GPAPDD296 pKa = 5.07DD297 pKa = 4.1AVADD301 pKa = 4.29APPAAPAFDD310 pKa = 4.73LPSEE314 pKa = 4.62HH315 pKa = 7.04PAASYY320 pKa = 10.25VLHH323 pKa = 6.66VNGADD328 pKa = 4.49RR329 pKa = 11.84PVTDD333 pKa = 3.02AWIGEE338 pKa = 4.15SLLYY342 pKa = 10.18VLRR345 pKa = 11.84EE346 pKa = 3.94RR347 pKa = 11.84LGLAGAKK354 pKa = 9.72DD355 pKa = 3.75GCSQGEE361 pKa = 4.12CGACNVQVDD370 pKa = 3.94GRR372 pKa = 11.84LVASCLVPAATAAGSEE388 pKa = 4.08VRR390 pKa = 11.84TVEE393 pKa = 3.9GLAVDD398 pKa = 4.82GEE400 pKa = 4.31PSDD403 pKa = 3.93VQRR406 pKa = 11.84ALADD410 pKa = 3.82CGAVQCGFCIPGMAMTVHH428 pKa = 7.14DD429 pKa = 5.06LLEE432 pKa = 4.83GNHH435 pKa = 6.43APSEE439 pKa = 4.33LEE441 pKa = 3.87TRR443 pKa = 11.84QALCGNLCRR452 pKa = 11.84CSGYY456 pKa = 10.39RR457 pKa = 11.84GVLDD461 pKa = 3.98AVNEE465 pKa = 4.35VIAGRR470 pKa = 11.84EE471 pKa = 3.92AASEE475 pKa = 4.1GAAAPEE481 pKa = 4.71PGSGDD486 pKa = 3.48PDD488 pKa = 3.86GARR491 pKa = 11.84IPHH494 pKa = 5.7QAAPGAGSVQAHH506 pKa = 5.78LQDD509 pKa = 3.62GGMAA513 pKa = 3.92

Molecular weight: 52.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S2UVC2|A0A4S2UVC2_9ACTN Aminoglycoside phosphotransferase OS=Streptomyces sp. A0642 OX=2563100 GN=E6R60_13265 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.5GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6934

0

6934

2322785

18

6264

335.0

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.739 ± 0.047

0.772 ± 0.009

5.942 ± 0.024

5.624 ± 0.033

2.731 ± 0.017

9.701 ± 0.029

2.271 ± 0.016

3.203 ± 0.019

2.2 ± 0.027

10.207 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.014

1.795 ± 0.018

6.088 ± 0.027

2.774 ± 0.019

7.828 ± 0.037

5.198 ± 0.023

6.167 ± 0.032

8.358 ± 0.028

1.519 ± 0.012

2.109 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski