Bactrocera dorsalis (Oriental fruit fly) (Dacus dorsalis)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13409 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I9V8K5|A0A6I9V8K5_BACDO probable cytochrome P450 12a5 mitochondrial OS=Bactrocera dorsalis OX=27457 GN=LOC105227637 PE=3 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.51 LLTVFVLLAAIVAALGDD19 pKa = 4.05 VPVNPDD25 pKa = 3.74 DD26 pKa = 5.49 SNVSTGDD33 pKa = 3.11 STTIAGTAVPDD44 pKa = 4.5 DD45 pKa = 4.77 SNTSASSNLLASTDD59 pKa = 3.34 TGTITSEE66 pKa = 3.91 PTTVPTGEE74 pKa = 4.3 TTSVATSEE82 pKa = 4.58 GTTSTSSPATTTVEE96 pKa = 3.88 PTTTVTTDD104 pKa = 2.87 EE105 pKa = 4.45 TTSVATSEE113 pKa = 4.14 EE114 pKa = 4.65 TTTVTTDD121 pKa = 3.15 EE122 pKa = 4.43 TASTSSPTTATTSSATSEE140 pKa = 4.39 TSSTTPSTTTTVAPSEE156 pKa = 4.4 TTPNTDD162 pKa = 2.71 EE163 pKa = 4.39 TTDD166 pKa = 3.2 ATTDD170 pKa = 3.14 EE171 pKa = 4.71 TTNEE175 pKa = 4.04 TTNATTNGTAASTATPDD192 pKa = 3.17 PTTPGNTTTVAPSEE206 pKa = 4.4 TTPNTDD212 pKa = 2.71 EE213 pKa = 4.39 TTDD216 pKa = 3.25 ATTDD220 pKa = 2.92 ATTNSTAASTVTPDD234 pKa = 3.25 TTPVQNGSTEE244 pKa = 3.96 APSANKK250 pKa = 8.92 LQCYY254 pKa = 9.43 ACEE257 pKa = 5.32 GDD259 pKa = 4.56 DD260 pKa = 4.16 CDD262 pKa = 4.0 EE263 pKa = 4.28 KK264 pKa = 10.83 TVINCPDD271 pKa = 3.83 DD272 pKa = 3.6 GRR274 pKa = 11.84 AYY276 pKa = 10.44 DD277 pKa = 4.44 VEE279 pKa = 4.44 DD280 pKa = 3.6 TSKK283 pKa = 11.2 SLSEE287 pKa = 3.96 NAAEE291 pKa = 5.17 ILLQNYY297 pKa = 9.48 RR298 pKa = 11.84 SVARR302 pKa = 11.84 LEE304 pKa = 3.98 LADD307 pKa = 3.62 VKK309 pKa = 11.11 GYY311 pKa = 10.9 ACYY314 pKa = 9.91 RR315 pKa = 11.84 VQITDD320 pKa = 3.7 NDD322 pKa = 3.91 DD323 pKa = 3.28 KK324 pKa = 11.46 ALKK327 pKa = 9.64 LGCITIPYY335 pKa = 8.49 GQSACEE341 pKa = 3.75 AVRR344 pKa = 11.84 NEE346 pKa = 4.13 LNITTTADD354 pKa = 3.18 SDD356 pKa = 3.67 EE357 pKa = 4.76 CLACQTDD364 pKa = 4.21 LCNGSAIAKK373 pKa = 9.95 LSVAVLLSVLLAKK386 pKa = 10.44 FLFF389 pKa = 4.42
Molecular weight: 39.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.579
IPC_protein 3.605
Toseland 3.376
ProMoST 3.757
Dawson 3.592
Bjellqvist 3.745
Wikipedia 3.516
Rodwell 3.427
Grimsley 3.287
Solomon 3.579
Lehninger 3.541
Nozaki 3.706
DTASelect 3.923
Thurlkill 3.427
EMBOSS 3.528
Sillero 3.719
Patrickios 1.151
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.689
Protein with the highest isoelectric point:
>tr|A0A6I9V844|A0A6I9V844_BACDO Gustatory receptor OS=Bactrocera dorsalis OX=27457 GN=LOC105223070 PE=3 SV=1
MM1 pKa = 7.55 PRR3 pKa = 11.84 NFFMFLFGVALLVVLITLAQAQPPTTRR30 pKa = 11.84 LISTTRR36 pKa = 11.84 RR37 pKa = 11.84 PTVRR41 pKa = 11.84 PTSTRR46 pKa = 11.84 RR47 pKa = 11.84 PIPPTRR53 pKa = 11.84 RR54 pKa = 11.84 PVPTRR59 pKa = 11.84 PRR61 pKa = 11.84 RR62 pKa = 11.84 PSPTRR67 pKa = 11.84 RR68 pKa = 11.84 PTPKK72 pKa = 9.87 RR73 pKa = 11.84 RR74 pKa = 11.84 PSS76 pKa = 3.03
Molecular weight: 8.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.403
IPC_protein 13.013
Toseland 13.173
ProMoST 13.671
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.656
Rodwell 12.705
Grimsley 13.217
Solomon 13.671
Lehninger 13.583
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.427
IPC_peptide 13.685
IPC2_peptide 12.676
IPC2.peptide.svr19 9.316
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11182
2227
13409
8119786
36
9118
605.5
67.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.297 ± 0.023
1.806 ± 0.015
5.222 ± 0.015
6.436 ± 0.026
3.533 ± 0.018
5.597 ± 0.025
2.584 ± 0.013
5.467 ± 0.017
6.012 ± 0.023
8.759 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.279 ± 0.008
5.616 ± 0.018
5.015 ± 0.025
4.94 ± 0.028
5.132 ± 0.017
8.247 ± 0.033
6.363 ± 0.023
5.749 ± 0.014
0.91 ± 0.007
3.029 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here