Acinetobacter phage 5W

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9YYZ9|A0A6M9YYZ9_9CAUD Uncharacterized protein OS=Acinetobacter phage 5W OX=2726968 PE=4 SV=1
MM1 pKa = 7.27NMLVNKK7 pKa = 10.11PEE9 pKa = 4.19LLCPSFPYY17 pKa = 10.87LDD19 pKa = 3.84MSTDD23 pKa = 3.11IQVEE27 pKa = 4.34GEE29 pKa = 4.31TVYY32 pKa = 11.12FDD34 pKa = 3.37LTYY37 pKa = 10.94GCNVLNCQIKK47 pKa = 11.01AEE49 pKa = 4.29TTYY52 pKa = 9.91DD53 pKa = 3.35TRR55 pKa = 11.84EE56 pKa = 3.92VTDD59 pKa = 3.41QFSGFARR66 pKa = 11.84DD67 pKa = 3.2QEE69 pKa = 4.63YY70 pKa = 10.66EE71 pKa = 4.22VLVVDD76 pKa = 4.39TRR78 pKa = 11.84THH80 pKa = 6.51AVVTDD85 pKa = 3.52KK86 pKa = 11.49DD87 pKa = 4.41GIEE90 pKa = 4.16SPIGLRR96 pKa = 11.84FKK98 pKa = 10.57LTDD101 pKa = 3.55AQVSSLNEE109 pKa = 3.59QLKK112 pKa = 10.81YY113 pKa = 10.35YY114 pKa = 10.73AEE116 pKa = 4.12EE117 pKa = 4.1LADD120 pKa = 3.62EE121 pKa = 4.44EE122 pKa = 4.93LRR124 pKa = 11.84GGG126 pKa = 3.66

Molecular weight: 14.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z2F6|A0A6M9Z2F6_9CAUD Uncharacterized protein OS=Acinetobacter phage 5W OX=2726968 PE=4 SV=1
MM1 pKa = 7.65SDD3 pKa = 3.53YY4 pKa = 11.03RR5 pKa = 11.84VDD7 pKa = 3.32AQVNFDD13 pKa = 3.59EE14 pKa = 4.53MNNRR18 pKa = 11.84VRR20 pKa = 11.84FEE22 pKa = 3.42IRR24 pKa = 11.84RR25 pKa = 11.84TINALTLRR33 pKa = 11.84LQRR36 pKa = 11.84IVQEE40 pKa = 4.75DD41 pKa = 3.95MLSGQRR47 pKa = 11.84LKK49 pKa = 10.94VQSGRR54 pKa = 11.84LRR56 pKa = 11.84GSVSSKK62 pKa = 10.13VDD64 pKa = 3.22EE65 pKa = 5.27DD66 pKa = 3.81KK67 pKa = 11.46DD68 pKa = 4.34SIEE71 pKa = 4.2GTVGAGGALVPYY83 pKa = 10.34APAHH87 pKa = 5.24EE88 pKa = 4.83FGLNGALGVKK98 pKa = 9.89AHH100 pKa = 6.41LRR102 pKa = 11.84TIKK105 pKa = 10.26QAFGRR110 pKa = 11.84PISPVQVNIKK120 pKa = 9.42AHH122 pKa = 4.94SRR124 pKa = 11.84NVRR127 pKa = 11.84FRR129 pKa = 11.84EE130 pKa = 3.81LRR132 pKa = 11.84FMRR135 pKa = 11.84DD136 pKa = 2.74SLDD139 pKa = 3.17IVAKK143 pKa = 10.3IVPKK147 pKa = 10.8NIDD150 pKa = 3.09AAIEE154 pKa = 3.87RR155 pKa = 11.84GIAGGG160 pKa = 3.43

Molecular weight: 17.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

13219

55

1007

216.7

24.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.784 ± 0.523

0.878 ± 0.121

5.885 ± 0.186

7.005 ± 0.3

4.199 ± 0.209

6.846 ± 0.304

1.732 ± 0.162

6.506 ± 0.235

7.709 ± 0.397

8.677 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.145

4.902 ± 0.248

3.616 ± 0.215

4.516 ± 0.264

4.289 ± 0.255

6.075 ± 0.222

5.727 ± 0.328

6.468 ± 0.231

1.157 ± 0.127

3.563 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski