Amino acid dipepetide frequency for Acinetobacter phage 5W

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.96AlaAla: 6.96 ± 1.143
0.378AlaCys: 0.378 ± 0.177
5.069AlaAsp: 5.069 ± 0.609
5.371AlaGlu: 5.371 ± 0.727
2.648AlaPhe: 2.648 ± 0.47
5.296AlaGly: 5.296 ± 0.74
1.437AlaHis: 1.437 ± 0.341
5.447AlaIle: 5.447 ± 0.764
7.036AlaLys: 7.036 ± 0.713
6.279AlaLeu: 6.279 ± 0.934
2.345AlaMet: 2.345 ± 0.479
3.404AlaAsn: 3.404 ± 0.57
2.572AlaPro: 2.572 ± 0.47
3.329AlaGln: 3.329 ± 0.586
3.253AlaArg: 3.253 ± 0.567
4.615AlaSer: 4.615 ± 0.53
4.085AlaThr: 4.085 ± 0.683
5.523AlaVal: 5.523 ± 0.592
0.757AlaTrp: 0.757 ± 0.3
1.967AlaTyr: 1.967 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.378CysAla: 0.378 ± 0.151
0.303CysCys: 0.303 ± 0.136
0.454CysAsp: 0.454 ± 0.216
1.21CysGlu: 1.21 ± 0.294
0.303CysPhe: 0.303 ± 0.138
0.908CysGly: 0.908 ± 0.255
0.076CysHis: 0.076 ± 0.077
0.303CysIle: 0.303 ± 0.137
0.53CysLys: 0.53 ± 0.182
0.303CysLeu: 0.303 ± 0.157
0.303CysMet: 0.303 ± 0.147
0.53CysAsn: 0.53 ± 0.185
0.227CysPro: 0.227 ± 0.135
0.53CysGln: 0.53 ± 0.208
0.757CysArg: 0.757 ± 0.237
0.151CysSer: 0.151 ± 0.095
0.53CysThr: 0.53 ± 0.2
0.53CysVal: 0.53 ± 0.173
0.151CysTrp: 0.151 ± 0.12
0.303CysTyr: 0.303 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
5.069AspAla: 5.069 ± 0.723
0.53AspCys: 0.53 ± 0.218
3.026AspAsp: 3.026 ± 0.527
4.464AspGlu: 4.464 ± 0.48
2.497AspPhe: 2.497 ± 0.384
4.237AspGly: 4.237 ± 0.626
1.059AspHis: 1.059 ± 0.251
3.858AspIle: 3.858 ± 0.532
4.464AspLys: 4.464 ± 0.505
6.279AspLeu: 6.279 ± 0.618
1.589AspMet: 1.589 ± 0.362
1.589AspAsn: 1.589 ± 0.278
1.967AspPro: 1.967 ± 0.366
2.799AspGln: 2.799 ± 0.396
1.362AspArg: 1.362 ± 0.297
4.085AspSer: 4.085 ± 0.472
3.556AspThr: 3.556 ± 0.526
3.858AspVal: 3.858 ± 0.54
0.681AspTrp: 0.681 ± 0.197
1.891AspTyr: 1.891 ± 0.376
0.0AspXaa: 0.0 ± 0.0
Glu
5.674GluAla: 5.674 ± 0.611
0.151GluCys: 0.151 ± 0.102
2.724GluAsp: 2.724 ± 0.464
4.918GluGlu: 4.918 ± 0.642
3.556GluPhe: 3.556 ± 0.475
4.539GluGly: 4.539 ± 0.495
0.908GluHis: 0.908 ± 0.247
4.539GluIle: 4.539 ± 0.586
5.523GluLys: 5.523 ± 0.815
7.112GluLeu: 7.112 ± 0.715
2.043GluMet: 2.043 ± 0.343
3.253GluAsn: 3.253 ± 0.468
1.589GluPro: 1.589 ± 0.447
3.934GluGln: 3.934 ± 0.553
3.48GluArg: 3.48 ± 0.404
3.556GluSer: 3.556 ± 0.545
3.253GluThr: 3.253 ± 0.555
4.842GluVal: 4.842 ± 0.637
1.059GluTrp: 1.059 ± 0.277
3.404GluTyr: 3.404 ± 0.509
0.0GluXaa: 0.0 ± 0.0
Phe
2.497PheAla: 2.497 ± 0.392
0.53PheCys: 0.53 ± 0.209
2.572PheAsp: 2.572 ± 0.4
3.404PheGlu: 3.404 ± 0.439
2.27PhePhe: 2.27 ± 0.376
3.177PheGly: 3.177 ± 0.512
0.53PheHis: 0.53 ± 0.234
3.48PheIle: 3.48 ± 0.654
3.253PheLys: 3.253 ± 0.453
2.875PheLeu: 2.875 ± 0.668
1.135PheMet: 1.135 ± 0.255
3.48PheAsn: 3.48 ± 0.564
1.135PhePro: 1.135 ± 0.286
1.437PheGln: 1.437 ± 0.333
1.513PheArg: 1.513 ± 0.36
2.875PheSer: 2.875 ± 0.493
1.891PheThr: 1.891 ± 0.353
2.27PheVal: 2.27 ± 0.41
0.303PheTrp: 0.303 ± 0.127
1.362PheTyr: 1.362 ± 0.354
0.0PheXaa: 0.0 ± 0.0
Gly
4.918GlyAla: 4.918 ± 0.753
0.605GlyCys: 0.605 ± 0.228
4.615GlyAsp: 4.615 ± 1.157
3.026GlyGlu: 3.026 ± 0.401
3.177GlyPhe: 3.177 ± 0.558
6.582GlyGly: 6.582 ± 0.755
1.135GlyHis: 1.135 ± 0.346
3.707GlyIle: 3.707 ± 0.492
5.523GlyLys: 5.523 ± 0.713
6.733GlyLeu: 6.733 ± 0.851
2.118GlyMet: 2.118 ± 0.343
3.026GlyAsn: 3.026 ± 0.52
1.059GlyPro: 1.059 ± 0.286
2.724GlyGln: 2.724 ± 0.525
3.329GlyArg: 3.329 ± 0.465
3.707GlySer: 3.707 ± 0.454
3.934GlyThr: 3.934 ± 0.617
4.918GlyVal: 4.918 ± 0.614
0.757GlyTrp: 0.757 ± 0.178
2.875GlyTyr: 2.875 ± 0.546
0.0GlyXaa: 0.0 ± 0.0
His
1.135HisAla: 1.135 ± 0.312
0.303HisCys: 0.303 ± 0.143
0.757HisAsp: 0.757 ± 0.208
1.286HisGlu: 1.286 ± 0.316
1.059HisPhe: 1.059 ± 0.312
1.059HisGly: 1.059 ± 0.227
0.454HisHis: 0.454 ± 0.154
1.664HisIle: 1.664 ± 0.361
0.984HisLys: 0.984 ± 0.278
2.043HisLeu: 2.043 ± 0.383
0.303HisMet: 0.303 ± 0.134
0.832HisAsn: 0.832 ± 0.203
0.908HisPro: 0.908 ± 0.258
0.908HisGln: 0.908 ± 0.279
0.378HisArg: 0.378 ± 0.151
0.681HisSer: 0.681 ± 0.223
0.53HisThr: 0.53 ± 0.214
1.286HisVal: 1.286 ± 0.305
0.303HisTrp: 0.303 ± 0.144
0.454HisTyr: 0.454 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
5.144IleAla: 5.144 ± 0.597
0.605IleCys: 0.605 ± 0.224
4.539IleAsp: 4.539 ± 0.698
5.901IleGlu: 5.901 ± 0.662
2.572IlePhe: 2.572 ± 0.45
5.069IleGly: 5.069 ± 0.669
1.135IleHis: 1.135 ± 0.323
2.951IleIle: 2.951 ± 0.603
5.674IleLys: 5.674 ± 0.688
4.766IleLeu: 4.766 ± 0.673
1.286IleMet: 1.286 ± 0.283
3.556IleAsn: 3.556 ± 0.63
2.345IlePro: 2.345 ± 0.386
3.026IleGln: 3.026 ± 0.492
2.572IleArg: 2.572 ± 0.384
3.556IleSer: 3.556 ± 0.467
3.707IleThr: 3.707 ± 0.484
4.01IleVal: 4.01 ± 0.587
0.681IleTrp: 0.681 ± 0.199
1.967IleTyr: 1.967 ± 0.417
0.0IleXaa: 0.0 ± 0.0
Lys
5.901LysAla: 5.901 ± 0.74
0.984LysCys: 0.984 ± 0.32
3.783LysAsp: 3.783 ± 0.503
6.204LysGlu: 6.204 ± 0.764
3.026LysPhe: 3.026 ± 0.536
4.085LysGly: 4.085 ± 0.412
1.437LysHis: 1.437 ± 0.283
5.069LysIle: 5.069 ± 0.564
5.598LysLys: 5.598 ± 0.813
6.658LysLeu: 6.658 ± 0.721
1.589LysMet: 1.589 ± 0.336
3.177LysAsn: 3.177 ± 0.451
3.48LysPro: 3.48 ± 0.532
4.691LysGln: 4.691 ± 0.539
4.01LysArg: 4.01 ± 0.549
4.539LysSer: 4.539 ± 0.834
4.01LysThr: 4.01 ± 0.584
4.464LysVal: 4.464 ± 0.509
0.908LysTrp: 0.908 ± 0.262
3.48LysTyr: 3.48 ± 0.46
0.0LysXaa: 0.0 ± 0.0
Leu
5.598LeuAla: 5.598 ± 0.793
0.832LeuCys: 0.832 ± 0.227
6.506LeuAsp: 6.506 ± 0.68
6.733LeuGlu: 6.733 ± 0.956
3.253LeuPhe: 3.253 ± 0.536
5.371LeuGly: 5.371 ± 0.572
1.286LeuHis: 1.286 ± 0.258
5.75LeuIle: 5.75 ± 0.936
7.792LeuLys: 7.792 ± 0.951
6.204LeuLeu: 6.204 ± 0.844
1.135LeuMet: 1.135 ± 0.239
4.993LeuAsn: 4.993 ± 0.724
3.404LeuPro: 3.404 ± 0.621
4.464LeuGln: 4.464 ± 0.494
3.329LeuArg: 3.329 ± 0.576
6.885LeuSer: 6.885 ± 0.608
4.766LeuThr: 4.766 ± 0.713
4.993LeuVal: 4.993 ± 0.47
0.832LeuTrp: 0.832 ± 0.234
2.648LeuTyr: 2.648 ± 0.446
0.0LeuXaa: 0.0 ± 0.0
Met
2.27MetAla: 2.27 ± 0.36
0.151MetCys: 0.151 ± 0.106
1.21MetAsp: 1.21 ± 0.258
0.832MetGlu: 0.832 ± 0.238
0.681MetPhe: 0.681 ± 0.179
1.816MetGly: 1.816 ± 0.353
0.378MetHis: 0.378 ± 0.182
0.832MetIle: 0.832 ± 0.225
2.345MetLys: 2.345 ± 0.525
2.194MetLeu: 2.194 ± 0.482
0.832MetMet: 0.832 ± 0.284
1.362MetAsn: 1.362 ± 0.302
1.135MetPro: 1.135 ± 0.318
0.908MetGln: 0.908 ± 0.201
1.059MetArg: 1.059 ± 0.251
2.572MetSer: 2.572 ± 0.461
1.589MetThr: 1.589 ± 0.352
1.513MetVal: 1.513 ± 0.303
0.303MetTrp: 0.303 ± 0.267
0.681MetTyr: 0.681 ± 0.214
0.0MetXaa: 0.0 ± 0.0
Asn
3.026AsnAla: 3.026 ± 0.431
0.605AsnCys: 0.605 ± 0.181
2.799AsnAsp: 2.799 ± 0.559
2.951AsnGlu: 2.951 ± 0.501
1.891AsnPhe: 1.891 ± 0.389
3.934AsnGly: 3.934 ± 0.457
1.21AsnHis: 1.21 ± 0.314
3.102AsnIle: 3.102 ± 0.526
2.724AsnLys: 2.724 ± 0.54
4.161AsnLeu: 4.161 ± 0.557
1.589AsnMet: 1.589 ± 0.389
2.345AsnAsn: 2.345 ± 0.573
2.497AsnPro: 2.497 ± 0.432
2.194AsnGln: 2.194 ± 0.387
2.421AsnArg: 2.421 ± 0.517
3.102AsnSer: 3.102 ± 0.596
3.026AsnThr: 3.026 ± 0.537
2.648AsnVal: 2.648 ± 0.469
0.757AsnTrp: 0.757 ± 0.254
2.043AsnTyr: 2.043 ± 0.352
0.0AsnXaa: 0.0 ± 0.0
Pro
2.572ProAla: 2.572 ± 0.4
0.227ProCys: 0.227 ± 0.127
2.118ProAsp: 2.118 ± 0.395
3.253ProGlu: 3.253 ± 0.621
1.589ProPhe: 1.589 ± 0.251
1.059ProGly: 1.059 ± 0.287
0.681ProHis: 0.681 ± 0.202
2.497ProIle: 2.497 ± 0.4
2.648ProLys: 2.648 ± 0.335
2.421ProLeu: 2.421 ± 0.365
0.681ProMet: 0.681 ± 0.196
1.74ProAsn: 1.74 ± 0.339
2.043ProPro: 2.043 ± 0.367
1.286ProGln: 1.286 ± 0.329
1.135ProArg: 1.135 ± 0.284
1.891ProSer: 1.891 ± 0.397
2.724ProThr: 2.724 ± 0.396
3.556ProVal: 3.556 ± 0.602
0.378ProTrp: 0.378 ± 0.15
1.664ProTyr: 1.664 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
4.539GlnAla: 4.539 ± 0.664
0.227GlnCys: 0.227 ± 0.122
1.589GlnAsp: 1.589 ± 0.358
2.648GlnGlu: 2.648 ± 0.402
1.74GlnPhe: 1.74 ± 0.427
2.572GlnGly: 2.572 ± 0.513
0.832GlnHis: 0.832 ± 0.259
3.329GlnIle: 3.329 ± 0.436
3.404GlnLys: 3.404 ± 0.501
4.464GlnLeu: 4.464 ± 0.627
1.286GlnMet: 1.286 ± 0.28
1.362GlnAsn: 1.362 ± 0.378
1.74GlnPro: 1.74 ± 0.396
2.421GlnGln: 2.421 ± 0.515
2.194GlnArg: 2.194 ± 0.406
3.102GlnSer: 3.102 ± 0.566
2.799GlnThr: 2.799 ± 0.622
2.875GlnVal: 2.875 ± 0.544
0.454GlnTrp: 0.454 ± 0.187
1.589GlnTyr: 1.589 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
3.707ArgAla: 3.707 ± 0.472
0.378ArgCys: 0.378 ± 0.201
2.27ArgAsp: 2.27 ± 0.398
2.497ArgGlu: 2.497 ± 0.373
2.043ArgPhe: 2.043 ± 0.389
2.27ArgGly: 2.27 ± 0.374
0.908ArgHis: 0.908 ± 0.28
3.48ArgIle: 3.48 ± 0.457
3.026ArgLys: 3.026 ± 0.392
4.464ArgLeu: 4.464 ± 0.457
1.135ArgMet: 1.135 ± 0.243
1.513ArgAsn: 1.513 ± 0.292
1.437ArgPro: 1.437 ± 0.3
1.74ArgGln: 1.74 ± 0.293
2.27ArgArg: 2.27 ± 0.409
2.194ArgSer: 2.194 ± 0.312
2.043ArgThr: 2.043 ± 0.408
2.724ArgVal: 2.724 ± 0.389
0.303ArgTrp: 0.303 ± 0.165
2.497ArgTyr: 2.497 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
4.539SerAla: 4.539 ± 0.624
0.454SerCys: 0.454 ± 0.183
4.766SerAsp: 4.766 ± 0.55
3.556SerGlu: 3.556 ± 0.451
2.648SerPhe: 2.648 ± 0.51
4.539SerGly: 4.539 ± 0.587
1.059SerHis: 1.059 ± 0.291
4.237SerIle: 4.237 ± 0.493
4.464SerLys: 4.464 ± 0.521
6.128SerLeu: 6.128 ± 0.671
1.286SerMet: 1.286 ± 0.259
3.48SerAsn: 3.48 ± 0.51
2.27SerPro: 2.27 ± 0.383
2.043SerGln: 2.043 ± 0.376
2.648SerArg: 2.648 ± 0.413
2.875SerSer: 2.875 ± 0.475
3.934SerThr: 3.934 ± 0.516
3.631SerVal: 3.631 ± 0.501
0.605SerTrp: 0.605 ± 0.178
1.589SerTyr: 1.589 ± 0.304
0.0SerXaa: 0.0 ± 0.0
Thr
5.371ThrAla: 5.371 ± 0.859
0.53ThrCys: 0.53 ± 0.196
3.329ThrAsp: 3.329 ± 0.559
3.707ThrGlu: 3.707 ± 0.58
2.497ThrPhe: 2.497 ± 0.426
4.085ThrGly: 4.085 ± 0.644
0.378ThrHis: 0.378 ± 0.156
4.01ThrIle: 4.01 ± 0.497
4.085ThrLys: 4.085 ± 0.539
4.388ThrLeu: 4.388 ± 0.647
1.135ThrMet: 1.135 ± 0.339
3.858ThrAsn: 3.858 ± 0.757
3.026ThrPro: 3.026 ± 0.608
1.74ThrGln: 1.74 ± 0.315
2.118ThrArg: 2.118 ± 0.354
3.329ThrSer: 3.329 ± 0.509
3.934ThrThr: 3.934 ± 0.708
3.556ThrVal: 3.556 ± 0.505
0.53ThrTrp: 0.53 ± 0.177
1.664ThrTyr: 1.664 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
4.766ValAla: 4.766 ± 0.666
0.53ValCys: 0.53 ± 0.155
3.707ValAsp: 3.707 ± 0.51
5.371ValGlu: 5.371 ± 0.626
2.497ValPhe: 2.497 ± 0.505
4.237ValGly: 4.237 ± 0.721
1.059ValHis: 1.059 ± 0.272
4.085ValIle: 4.085 ± 0.624
4.993ValLys: 4.993 ± 0.587
4.766ValLeu: 4.766 ± 0.583
1.816ValMet: 1.816 ± 0.407
3.48ValAsn: 3.48 ± 0.59
2.27ValPro: 2.27 ± 0.457
2.724ValGln: 2.724 ± 0.514
3.102ValArg: 3.102 ± 0.571
4.01ValSer: 4.01 ± 0.414
4.237ValThr: 4.237 ± 0.523
4.161ValVal: 4.161 ± 0.538
0.832ValTrp: 0.832 ± 0.394
2.043ValTyr: 2.043 ± 0.388
0.0ValXaa: 0.0 ± 0.0
Trp
1.059TrpAla: 1.059 ± 0.292
0.076TrpCys: 0.076 ± 0.068
0.605TrpAsp: 0.605 ± 0.202
0.832TrpGlu: 0.832 ± 0.205
0.832TrpPhe: 0.832 ± 0.256
0.454TrpGly: 0.454 ± 0.215
0.53TrpHis: 0.53 ± 0.189
0.908TrpIle: 0.908 ± 0.245
0.757TrpLys: 0.757 ± 0.202
1.286TrpLeu: 1.286 ± 0.267
0.227TrpMet: 0.227 ± 0.148
0.53TrpAsn: 0.53 ± 0.258
0.303TrpPro: 0.303 ± 0.162
0.303TrpGln: 0.303 ± 0.143
0.378TrpArg: 0.378 ± 0.161
0.984TrpSer: 0.984 ± 0.277
0.227TrpThr: 0.227 ± 0.115
0.681TrpVal: 0.681 ± 0.281
0.227TrpTrp: 0.227 ± 0.146
0.378TrpTyr: 0.378 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.724TyrAla: 2.724 ± 0.564
0.378TyrCys: 0.378 ± 0.187
2.421TyrAsp: 2.421 ± 0.383
1.891TyrGlu: 1.891 ± 0.356
1.21TyrPhe: 1.21 ± 0.359
3.177TyrGly: 3.177 ± 0.537
0.832TyrHis: 0.832 ± 0.258
1.816TyrIle: 1.816 ± 0.427
2.27TyrLys: 2.27 ± 0.38
3.253TyrLeu: 3.253 ± 0.553
0.757TyrMet: 0.757 ± 0.205
1.513TyrAsn: 1.513 ± 0.351
0.757TyrPro: 0.757 ± 0.255
1.967TyrGln: 1.967 ± 0.353
1.589TyrArg: 1.589 ± 0.334
2.043TyrSer: 2.043 ± 0.352
2.497TyrThr: 2.497 ± 0.44
2.648TyrVal: 2.648 ± 0.364
0.757TyrTrp: 0.757 ± 0.202
1.135TyrTyr: 1.135 ± 0.279
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (13219 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski