Rhinocladiella mackenziei CBS 650.93

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Rhinocladiella; Rhinocladiella mackenziei

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11378 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2IUW8|A0A0D2IUW8_9EURO Rhinocladiella mackenziei CBS 650.93 unplaced genomic scaffold supercont1.1 whole genome shotgun sequence OS=Rhinocladiella mackenziei CBS 650.93 OX=1442369 GN=Z518_00965 PE=4 SV=1
MM1 pKa = 7.75HH2 pKa = 7.39SFTVLLAVSAFASHH16 pKa = 6.72SLAKK20 pKa = 9.83PIPASDD26 pKa = 3.22VSTKK30 pKa = 10.59KK31 pKa = 10.88GFTVKK36 pKa = 10.25QAIAKK41 pKa = 7.52PFQAGPVVLQKK52 pKa = 9.76TYY54 pKa = 11.11NKK56 pKa = 8.92YY57 pKa = 6.29TTAVPADD64 pKa = 3.91VKK66 pKa = 10.93AAAADD71 pKa = 4.04GSVTATPEE79 pKa = 3.97QYY81 pKa = 10.38DD82 pKa = 3.78AEE84 pKa = 4.38YY85 pKa = 10.32LSPVSIGGQTLNLDD99 pKa = 4.16FDD101 pKa = 4.24TGSADD106 pKa = 2.96LWVFSSEE113 pKa = 4.48LPSSQQSGHH122 pKa = 6.24SIYY125 pKa = 10.89DD126 pKa = 3.41PSKK129 pKa = 10.68SDD131 pKa = 3.2TAKK134 pKa = 10.35EE135 pKa = 3.96LEE137 pKa = 4.22GATWNISYY145 pKa = 11.03GDD147 pKa = 3.67GSGASGNVYY156 pKa = 10.06TDD158 pKa = 3.39TVDD161 pKa = 3.18VGGTTVTGQAVEE173 pKa = 4.31LAAQISAQFQQDD185 pKa = 3.53EE186 pKa = 4.68NNDD189 pKa = 3.65GLLGLAFSSINTVQPDD205 pKa = 3.47QQTTFFDD212 pKa = 3.56TAIQQGVLSQNLFTVDD228 pKa = 3.9LKK230 pKa = 11.06KK231 pKa = 11.08GEE233 pKa = 4.41PGTYY237 pKa = 10.33DD238 pKa = 3.37FGFIDD243 pKa = 4.49DD244 pKa = 4.63SKK246 pKa = 9.85HH247 pKa = 5.86TGDD250 pKa = 3.05ITYY253 pKa = 9.54TPVDD257 pKa = 3.53TANGFWEE264 pKa = 4.71FTGTGYY270 pKa = 11.33AVGDD274 pKa = 3.91GSFQQQSIDD283 pKa = 3.95AIADD287 pKa = 3.46TGTTLLLMDD296 pKa = 5.35DD297 pKa = 5.07SIVSAYY303 pKa = 10.12YY304 pKa = 10.47DD305 pKa = 3.31QVDD308 pKa = 3.61GAKK311 pKa = 10.19YY312 pKa = 10.87DD313 pKa = 3.85NTQGGYY319 pKa = 7.62TFPCSADD326 pKa = 3.45LPDD329 pKa = 3.85FAVGIEE335 pKa = 4.78DD336 pKa = 3.53YY337 pKa = 10.85HH338 pKa = 8.3AVIPGSFMNFAPADD352 pKa = 3.49SSTCYY357 pKa = 10.5GGLQSNEE364 pKa = 4.07GIGFSIYY371 pKa = 11.02GDD373 pKa = 3.29IFLKK377 pKa = 10.87AVFAVFDD384 pKa = 4.03SDD386 pKa = 3.46NTQLGFAPKK395 pKa = 10.41DD396 pKa = 3.43LL397 pKa = 4.54

Molecular weight: 41.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2J7K2|A0A0D2J7K2_9EURO Rhinocladiella mackenziei CBS 650.93 unplaced genomic scaffold supercont1.4 whole genome shotgun sequence OS=Rhinocladiella mackenziei CBS 650.93 OX=1442369 GN=Z518_05877 PE=4 SV=1
MM1 pKa = 7.53ALGRR5 pKa = 11.84RR6 pKa = 11.84TRR8 pKa = 11.84HH9 pKa = 5.74HH10 pKa = 6.8SSGTTVTTTTTTTHH24 pKa = 6.64HH25 pKa = 6.68PPGTKK30 pKa = 9.84VSPSGQVRR38 pKa = 11.84TKK40 pKa = 8.57PTFMQRR46 pKa = 11.84LRR48 pKa = 11.84GNTTPRR54 pKa = 11.84TTTARR59 pKa = 11.84PARR62 pKa = 11.84QRR64 pKa = 11.84RR65 pKa = 11.84WGGTRR70 pKa = 11.84TRR72 pKa = 11.84RR73 pKa = 11.84TPATATTPSRR83 pKa = 11.84KK84 pKa = 8.13TSIGDD89 pKa = 3.66KK90 pKa = 10.84VSGAMLKK97 pKa = 10.78LKK99 pKa = 9.49GTVTRR104 pKa = 11.84RR105 pKa = 11.84PGQKK109 pKa = 9.82AAGTRR114 pKa = 11.84RR115 pKa = 11.84MHH117 pKa = 5.82GTDD120 pKa = 3.0GSGTRR125 pKa = 11.84RR126 pKa = 11.84YY127 pKa = 10.03

Molecular weight: 13.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11378

0

11378

5555827

50

7334

488.3

54.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.257 ± 0.018

1.228 ± 0.008

5.714 ± 0.015

6.164 ± 0.018

3.789 ± 0.013

6.738 ± 0.02

2.446 ± 0.01

5.086 ± 0.015

4.83 ± 0.018

8.968 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.009

3.697 ± 0.01

6.045 ± 0.021

4.05 ± 0.014

6.237 ± 0.017

8.2 ± 0.026

5.979 ± 0.014

6.089 ± 0.014

1.484 ± 0.009

2.777 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski